Figure 2  Optimization of screening parameters: (A) Maximizing sensitivity through the effect of motion, incubation time, ligand spotting concentration, and the number of panned cells using a cRGDyK array and a mixture of M21 and M21-L cells. Shown are mean ± SD for each data point from 4 randomly chosen spots on the slide. (B) Maximizing specificity through the use of serum or competing receptor-negative cells.