Figure 4  Knockdown of eEF1A increases CSFV growth. (A) CSFV genome copies in eEF1A knockdown cells. PK-15 cells transduced with the lenti-sheEF1A or lenti-NC for 24 h were infected with the CSFV Shimen strain at a multiplicity of infection (MOI) of 0.1 for 48 h or 72 h. The CSFV genome copy numbers were assessed using a real-time RT-PCR assay as described previously [25]. (B) CSFV titers in eEF1A-knocked down cells. Virus titers in the supernatant were determined at 48 and 72 h post-infection (hpi) and expressed as 50% tissue culture infective doses (TCID50)/mL. Error bars represent the standard deviations of the means from three independent experiments. p-values were indicated above the bars. (C) Knockdown of eEF1A by lentivirus-mediated shRNAs increased expression of the CSFV Npro protein. The endogenous eEF1A and the CSFV Npro protein were detected by Western blotting using an anti-eEF1A monoclonal antibody (MAb) and an anti-Npro polyclonal antibody (PAb), respectively.