Figure 3  Overexpression of eEF1A suppresses the CSFV growth. (A and B) Western blotting analysis of overexpressed EGFP-eEF1A and CSFV Npro. PK-15 cells were transduced with the lentivirus expressing EGFP-eEF1A or EGFP, followed by infection with CSFV at a multiplicity of infection (MOI) of 0.1. Protein expression was examined by Western blotting at 48 (A) and 72 h (B) post-infection (hpi) using a rabbit anti-eEF1A monoclonal antibody (MAb) (1:2000) and a mouse anti-Npro polyclonal antibody (PAb) (1:500) (produced in-house). β-Tubulin served as an internal control; (C) Real-time RT-PCR analysis of CSFV genomic copy numbers. The culture supernatant of the CSFV-infected cells at 48 and 72 hpi were harvested and evaluated for the viral genomic copy numbers by real-time RT-PCR as described previously [25]. (D) CSFV titers in eEF1A-overexpressed cells. Virus titers in the supernatant were determined at 48 and 72 hpi and expressed as 50% tissue culture infective doses (TCID50)/mL. Error bars represent the standard deviations of the means from three independent experiments. p-values were indicated above the bars.
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