(D) Separation and detection of RT-PEx reactions on HeLa cells-derived RNA following a 6-day siRNA-mediated depletion of TRMT5. Two different siRNA were used (HSS126475 and HSS126476, Life Technologies). Untransfected cells and cells transfected with siRNA against GFP (Dsc-1001, Lonza) were used as controls. Quantification values displayed as per (B).