PMC:4573257 / 30977-32203 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4573257","sourcedb":"PMC","sourceid":"4573257","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4573257","text":"Mitochondrial Localization of TRMT5\n(A) Localization of TRMT5 by immunofluorescence in HeLa cells following the transient expression of a C-terminal FLAG-tagged TRMT5 construct (TRMT5-FLAG). Cell nuclei were stained with DAPI (top left). TRMT5-FLAG was detected by an anti-FLAG antibody (top right). We detected mitochondria by targeting a known mitochondrial protein, TOM20, with an anti-TOM20 antibody (bottom left). We digitally merged the preceding images, and signal overlap between TRMT5-FLAG and TOM20 appears yellow (bottom right).\n(B) Localization of TRMT5 in sub-cellular fractions. HeLa cells were fractionated into debris (D, lane 2), cytosol (C, lane 3), and mitochondria (C, lanes 4–6). The mitochondrial fraction was treated with 25 μg/ml proteinase K in the absence (C, lane 5) or presence (C, lane 6) of 1% Triton X-100. The fractions were analyzed by western blotting with antibodies against TRMT5 (HPA000943, Sigma). The distribution of TRMT5 was compared with that of the following marker proteins: mtSSB1 (mitochondrial matrix), TOM22 (mitochondrial outer membrane), GAPDH (cytosol), and Histone H4 (nucleus). Abbreviations are as follows: T, total cell lysate; fl, full-length TOM22; tr, truncated TOM22.","divisions":[{"label":"p","span":{"begin":0,"end":35}},{"label":"p","span":{"begin":36,"end":539}}],"tracks":[]}