PMC:4573257 / 19734-20543 JSONTXT

{"project":"2_test","denotations":[{"id":"26189817-23313956-2053347","span":{"begin":305,"end":307},"obj":"23313956"}],"text":"In order to confirm that defects in TRMT5 are the cause of the aberrant modification of G37 in mt-tRNALeu(CUN), we transduced the 65205, 73901, and control primary fibroblasts with a wild-type copy of TRMT5 cDNA by using a lentiviral vector (pLenti 6.3/V5 TOPO, Life Technologies) as previously described.37 The transduction had no noticeable effect on the G37 modification status of mt-tRNALeu(CUN) in the control-cell line (C2-T, Figure 5A). However, in transduced 73901 fibroblasts, the expression of wild-type TRMT5 was found to reverse the hypomodification effect observed in fibroblasts from the affected individuals, resulting in m1G37 amounts matching those of the control individual (73901-T, Figure 5A). We were unable to recover any viable cells from subject 65205 after the transduction procedure."}