Figure 5  Validation of the yeast microarray by real-time rtPCR in HEK293T cells. (A) Gene expression 12 h post-transfection in human HEK293T cells of three selected genes. (B) Gene expression 12, 20, and 24 h post-transfection with either wtDFNA5 or mutant DFNA5. UCP2, uncoupling protein 2 (mitochondrial, proton carrier); TM7SF2, transmembrane 7 superfamily member 2; VPS33B, vacuolar protein sorting 33 homolog. Light gray, wtDFNA5; Dark gray, mutDFNA5. *p < 0.05. CNRQ, calibrated normalized relative quantities.