PMC:4504098 / 8192-9232 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4504098","sourcedb":"PMC","sourceid":"4504098","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4504098","text":"Of the Subic Bay samples, four sera were potentially positive on ELISA: three from Acerodon jubatus (s9, s21, s57), and one from Pteropus vampyrus (s53). Three (s9, s21, s57) were also positive on Western blot (Table 3). One sample (s57) showed a stronger response to EBOV than to RESTV antigen (Fig. 4). All samples and swabs were negative for RESTV RNA on qPCR.\nTable 3 Positive serologic findings in 61 flying-foxesa screened for anti-RESTV antibodies by ELISA and Western blot\nBat/sample ID Species Locality ELISA Western blot\ns9 A. jubatus Subic Bay + +\ns21 A. jubatus Subic Bay + +\ns53 P. vampyrus Tala +\ns57 A. jubatus Subic Bay + +b\naComprising 56 A. jubatus and 5 P. vampyrus from two locations\nbs57 showed a stronger reactivity to EBOV than to RESTV\nFig. 4 Western blot analysis. Recombinant nucleoproteins from RESTV (rN) and EBOV (zN) were used to probe for reactivity in four ELISA positive sera (s9, s21, s53 and s57) and one ELISA negative serum (s14). Anti-His tag monoclonal antibody (H) was used as a positive control\n","divisions":[{"label":"table-wrap","span":{"begin":364,"end":764}},{"label":"label","span":{"begin":364,"end":371}},{"label":"caption","span":{"begin":372,"end":480}},{"label":"p","span":{"begin":372,"end":480}},{"label":"table","span":{"begin":481,"end":644}},{"label":"tr","span":{"begin":481,"end":530}},{"label":"th","span":{"begin":481,"end":494}},{"label":"th","span":{"begin":495,"end":502}},{"label":"th","span":{"begin":503,"end":511}},{"label":"th","span":{"begin":512,"end":517}},{"label":"th","span":{"begin":518,"end":530}},{"label":"tr","span":{"begin":531,"end":559}},{"label":"td","span":{"begin":531,"end":533}},{"label":"td","span":{"begin":534,"end":545}},{"label":"td","span":{"begin":546,"end":555}},{"label":"td","span":{"begin":556,"end":557}},{"label":"td","span":{"begin":558,"end":559}},{"label":"tr","span":{"begin":560,"end":589}},{"label":"td","span":{"begin":560,"end":563}},{"label":"td","span":{"begin":564,"end":575}},{"label":"td","span":{"begin":576,"end":585}},{"label":"td","span":{"begin":586,"end":587}},{"label":"td","span":{"begin":588,"end":589}},{"label":"tr","span":{"begin":590,"end":613}},{"label":"td","span":{"begin":590,"end":593}},{"label":"td","span":{"begin":594,"end":606}},{"label":"td","span":{"begin":607,"end":611}},{"label":"td","span":{"begin":612,"end":613}},{"label":"tr","span":{"begin":614,"end":644}},{"label":"td","span":{"begin":614,"end":617}},{"label":"td","span":{"begin":618,"end":629}},{"label":"td","span":{"begin":630,"end":639}},{"label":"td","span":{"begin":640,"end":641}},{"label":"td","span":{"begin":642,"end":644}},{"label":"table-wrap-foot","span":{"begin":645,"end":764}},{"label":"p","span":{"begin":645,"end":708}},{"label":"p","span":{"begin":708,"end":764}},{"label":"label","span":{"begin":765,"end":771}}],"tracks":[]}