Of the Subic Bay samples, four sera were potentially positive on ELISA: three from Acerodon jubatus (s9, s21, s57), and one from Pteropus vampyrus (s53). Three (s9, s21, s57) were also positive on Western blot (Table 3). One sample (s57) showed a stronger response to EBOV than to RESTV antigen (Fig. 4). All samples and swabs were negative for RESTV RNA on qPCR.
Table 3 Positive serologic findings in 61 flying-foxesa screened for anti-RESTV antibodies by ELISA and Western blot
Bat/sample ID Species Locality ELISA Western blot
s9  A. jubatus Subic Bay + +
s21  A. jubatus Subic Bay + +
s53  P. vampyrus Tala +
s57  A. jubatus Subic Bay + +b
aComprising 56 A. jubatus and 5 P. vampyrus from two locations
bs57 showed a stronger reactivity to EBOV than to RESTV
Fig. 4 Western blot analysis. Recombinant nucleoproteins from RESTV (rN) and EBOV (zN) were used to probe for reactivity in four ELISA positive sera (s9, s21, s53 and s57) and one ELISA negative serum (s14). Anti-His tag monoclonal antibody (H) was used as a positive control