Table 2 qPCR results on original and archived PAHC duplicate oropharangeal swabs from five pools screening potentially positivea
Pool Animal ID Species Location Original sample pool Ct Original sample: individual Ct Duplicate sample: individual Ct
2 U95  C. brachyotis Puning Cave 42.0/ND 41.2/ND
U96  Pt. jagori Puning Cave
U97  Pt. jagori Puning Cave
U98  Pt. jagori Puning Cave
U99  Pt. jagori Puning Cave
3 R1  M. australis Biak na Bato 43.0/ND
R2  M. australis Biak na Bato
R3  M. australis Biak na Bato
R4  M. australis Biak na Bato 39.3/ND
R5  M. australis Biak na Bato
4 T67  M. australis Biak na Bato 40.6/41.9b
T68  M. australis Biak na Bato
T69  M. schreibersii c Biak na Bato 33.6d
T70  M. schreibersii c Biak na Bato 37.7d 39.9/ND
T71  M. schreibersii c Biak na Bato 32.9d 40.1/ND
T72  M. schreibersii e Biak na Bato
T73  M. schreibersii e Biak na Bato
T74  M. schreibersii e Biak na Bato 40.5/ND
T75  M. schreibersii e Biak na Bato
T76  M. schreibersii e Biak na Bato
6 T56  Ch. plicata Biak na Bato 39.7/40.1b
T57  Ch. plicata Biak na Bato
T58  Ch. plicata Biak na Bato
T59  Ch. plicata Biak na Bato
T60  M. schreibersii Biak na Bato
12 U21  M. australis Biak na Bato 40.2/ND 42.2/ND
U22  M. australis Biak na Bato
U23  Ch. plicata Biak na Bato
U24  Ch. plicata Biak na Bato
U25  Ch. plicata Biak na Bato
aAll samples were tested in duplicate. Positive samples were confirmed in triplicate
bPools 4 and 6 had repeatable results on the original pooled sample
cT69, T70 and T71 yielded a product on hemi-nested PCR whose sequence differed by one nucleotide from a pig isolate in Bulacan province
dMean value of the duplicates
eAdditional M. schreibersii samples from a pool which tested negative in the original round