Figure 3  RPO26 and RPO31 are dosage suppressors of tgs1Δ cold sensitivity. DTS1 (8.6 kb) and DTS2 (12.4 kb) are the two genomic inserts in the 2-μ URA3 plasmids that were isolated in the dosage suppressor screen for reversal of tgs1Δ cs growth. Individual genes with complete ORFs within the DTS1 and DTS2 inserts including PZF1, RPO26, MLC2, SKI3, AZF1, RPO31, TRS33, and YOR114w were cloned into a 2-μ URA3 vector. These plasmids, an empty CEN URA3 vector (negative control), a CEN URA3 plasmid containing wild-type TGS1 (positive control), and the plasmids containing DTS1 or DTS2 were transformed into tgs1Δ cells. Ura+ transformants were selected and grown at 30° in liquid medium lacking uracil. The cultures were adjusted to an A600 of 0.1 and aliquots of serial 10-fold dilutions were spotted on agar medium lacking uracil. The plates were photographed after incubation for 7 d at 18°.