Id |
Subject |
Object |
Predicate |
Lexical cue |
T10440 |
8-10 |
IN |
denotes |
of |
T10441 |
11-21 |
NN |
denotes |
vertebrate |
T10442 |
22-26 |
NN |
denotes |
TACC |
T10443 |
27-32 |
NNS |
denotes |
cDNAs |
T10444 |
32-297 |
sentence |
denotes |
The rabbit TACC3 was cloned by rt-PCR using the "TACC4" specific primer T4RACE2 [9] (5'-cccgaactgctccaggtaatcgatctc-3') and a consensus primer, T3con2, designed to the region encompassing the vertebrate TACC3 initiator methionine (5'-tatgagtctgcaggtcttaaacgac-3'). |
T10445 |
33-36 |
DT |
denotes |
The |
T10446 |
44-49 |
NN |
denotes |
TACC3 |
T10447 |
37-43 |
NN |
denotes |
rabbit |
T10448 |
54-60 |
VBN |
denotes |
cloned |
T10449 |
50-53 |
VBD |
denotes |
was |
T10450 |
61-63 |
IN |
denotes |
by |
T10451 |
64-66 |
NN |
denotes |
rt |
T10452 |
67-70 |
NN |
denotes |
PCR |
T10453 |
66-67 |
HYPH |
denotes |
- |
T10454 |
71-76 |
VBG |
denotes |
using |
T10455 |
77-80 |
DT |
denotes |
the |
T10456 |
98-104 |
NN |
denotes |
primer |
T10457 |
81-82 |
`` |
denotes |
" |
T10458 |
89-97 |
JJ |
denotes |
specific |
T10459 |
82-87 |
NN |
denotes |
TACC4 |
T10460 |
87-88 |
'' |
denotes |
" |
T10461 |
105-112 |
NN |
denotes |
T4RACE2 |
T10462 |
113-114 |
-LRB- |
denotes |
[ |
T10463 |
114-115 |
CD |
denotes |
9 |
T10464 |
115-116 |
-RRB- |
denotes |
] |
T10465 |
117-118 |
-LRB- |
denotes |
( |
T10466 |
121-148 |
NN |
denotes |
cccgaactgctccaggtaatcgatctc |
T10467 |
118-119 |
CD |
denotes |
5 |
T10468 |
119-120 |
SYM |
denotes |
' |
T10469 |
120-121 |
HYPH |
denotes |
- |
T10470 |
148-149 |
HYPH |
denotes |
- |
T10471 |
149-150 |
CD |
denotes |
3 |
T10472 |
150-151 |
SYM |
denotes |
' |
T10473 |
151-152 |
-RRB- |
denotes |
) |
T10474 |
153-156 |
CC |
denotes |
and |
T10475 |
157-158 |
DT |
denotes |
a |
T10476 |
169-175 |
NN |
denotes |
primer |
T10477 |
159-168 |
NN |
denotes |
consensus |
T10478 |
175-177 |
, |
denotes |
, |
T10479 |
177-183 |
NN |
denotes |
T3con2 |
T10480 |
183-185 |
, |
denotes |
, |
T10481 |
185-193 |
VBN |
denotes |
designed |
T10482 |
194-196 |
IN |
denotes |
to |
T10483 |
197-200 |
DT |
denotes |
the |
T10484 |
201-207 |
NN |
denotes |
region |
T10485 |
208-220 |
VBG |
denotes |
encompassing |
T10486 |
221-224 |
DT |
denotes |
the |
T10487 |
242-251 |
NN |
denotes |
initiator |
T10488 |
225-235 |
NN |
denotes |
vertebrate |
T10489 |
236-241 |
NN |
denotes |
TACC3 |
T10490 |
252-262 |
NN |
denotes |
methionine |
T10491 |
263-264 |
-LRB- |
denotes |
( |
T10492 |
267-292 |
NN |
denotes |
tatgagtctgcaggtcttaaacgac |
T10493 |
264-265 |
CD |
denotes |
5 |
T10494 |
265-266 |
SYM |
denotes |
' |
T10495 |
266-267 |
HYPH |
denotes |
- |
T10496 |
292-293 |
HYPH |
denotes |
- |
T10497 |
293-294 |
CD |
denotes |
3 |
T10498 |
294-295 |
SYM |
denotes |
' |
T10499 |
295-296 |
-RRB- |
denotes |
) |
T10500 |
296-297 |
. |
denotes |
. |
T10501 |
297-683 |
sentence |
denotes |
For cloning the mouse Tacc1X cDNA, the primers used were based upon the genomic sequence reported, and the sequence of the IMAGE cDNA clone 4933429K08: T1XF (5'-ccatgttcagtcattggcaggtc-3'), T1XF2 (5'-ctgcagaaccaacagttcaag-3'), T1XR1 (5'-agatctgtgacatcacagctc-3'), T1XR2 (5'-ctcgagtcagttagtcttatccagctt-3'), BB617F (5'-accaccaacttgagtacctg-3') and BB617R (5'-gtatcttgaactgttggttctg-3'). |
T10502 |
298-301 |
IN |
denotes |
For |
T10503 |
355-360 |
VBN |
denotes |
based |
T10504 |
302-309 |
VBG |
denotes |
cloning |
T10505 |
310-313 |
DT |
denotes |
the |
T10506 |
327-331 |
NN |
denotes |
cDNA |
T10507 |
314-319 |
NN |
denotes |
mouse |
T10508 |
320-326 |
NN |
denotes |
Tacc1X |
T10509 |
331-333 |
, |
denotes |
, |
T10510 |
333-336 |
DT |
denotes |
the |
T10511 |
337-344 |
NNS |
denotes |
primers |
T10512 |
345-349 |
VBN |
denotes |
used |
T10513 |
350-354 |
VBD |
denotes |
were |
T10514 |
361-365 |
IN |
denotes |
upon |
T10515 |
366-369 |
DT |
denotes |
the |
T10516 |
378-386 |
NN |
denotes |
sequence |
T10517 |
370-377 |
JJ |
denotes |
genomic |
T10518 |
387-395 |
VBN |
denotes |
reported |
T10519 |
395-397 |
, |
denotes |
, |
T10520 |
397-400 |
CC |
denotes |
and |
T10521 |
401-404 |
DT |
denotes |
the |
T10522 |
405-413 |
NN |
denotes |
sequence |
T10523 |
414-416 |
IN |
denotes |
of |
T10524 |
417-420 |
DT |
denotes |
the |
T10525 |
432-437 |
NN |
denotes |
clone |
T10526 |
421-426 |
NN |
denotes |
IMAGE |
T10527 |
427-431 |
NN |
denotes |
cDNA |
T10528 |
438-448 |
NN |
denotes |
4933429K08 |
T10529 |
448-450 |
: |
denotes |
: |
T10530 |
450-454 |
NN |
denotes |
T1XF |
T10531 |
455-456 |
-LRB- |
denotes |
( |
T10532 |
459-482 |
NN |
denotes |
ccatgttcagtcattggcaggtc |
T10533 |
456-457 |
CD |
denotes |
5 |
T10534 |
457-458 |
SYM |
denotes |
' |
T10535 |
458-459 |
HYPH |
denotes |
- |
T10536 |
482-483 |
HYPH |
denotes |
- |
T10537 |
483-484 |
CD |
denotes |
3 |
T10538 |
484-485 |
SYM |
denotes |
' |
T10539 |
485-486 |
-RRB- |
denotes |
) |
T10540 |
486-488 |
, |
denotes |
, |
T10541 |
488-493 |
NN |
denotes |
T1XF2 |
T10542 |
494-495 |
-LRB- |
denotes |
( |
T10543 |
498-519 |
NN |
denotes |
ctgcagaaccaacagttcaag |
T10544 |
495-496 |
CD |
denotes |
5 |
T10545 |
496-497 |
SYM |
denotes |
' |
T10546 |
497-498 |
HYPH |
denotes |
- |
T10547 |
519-520 |
HYPH |
denotes |
- |
T10548 |
520-521 |
CD |
denotes |
3 |
T10549 |
521-522 |
SYM |
denotes |
' |
T10550 |
522-523 |
-RRB- |
denotes |
) |
T10551 |
523-525 |
, |
denotes |
, |
T10552 |
525-530 |
NN |
denotes |
T1XR1 |
T10553 |
531-532 |
-LRB- |
denotes |
( |
T10554 |
535-556 |
NN |
denotes |
agatctgtgacatcacagctc |
T10555 |
532-533 |
CD |
denotes |
5 |
T10556 |
533-534 |
SYM |
denotes |
' |
T10557 |
534-535 |
HYPH |
denotes |
- |
T10558 |
556-557 |
HYPH |
denotes |
- |
T10559 |
557-558 |
CD |
denotes |
3 |
T10560 |
558-559 |
SYM |
denotes |
' |
T10561 |
559-560 |
-RRB- |
denotes |
) |
T10562 |
560-562 |
, |
denotes |
, |
T10563 |
562-567 |
NN |
denotes |
T1XR2 |
T10564 |
568-569 |
-LRB- |
denotes |
( |
T10565 |
572-599 |
NN |
denotes |
ctcgagtcagttagtcttatccagctt |
T10566 |
569-570 |
CD |
denotes |
5 |
T10567 |
570-571 |
SYM |
denotes |
' |
T10568 |
571-572 |
HYPH |
denotes |
- |
T10569 |
599-600 |
HYPH |
denotes |
- |
T10570 |
600-601 |
CD |
denotes |
3 |
T10571 |
601-602 |
SYM |
denotes |
' |
T10572 |
602-603 |
-RRB- |
denotes |
) |
T10573 |
603-605 |
, |
denotes |
, |
T10574 |
605-611 |
NN |
denotes |
BB617F |
T10575 |
612-613 |
-LRB- |
denotes |
( |
T10576 |
616-636 |
NN |
denotes |
accaccaacttgagtacctg |
T10577 |
613-614 |
CD |
denotes |
5 |
T10578 |
614-615 |
SYM |
denotes |
' |
T10579 |
615-616 |
HYPH |
denotes |
- |
T10580 |
636-637 |
HYPH |
denotes |
- |
T10581 |
637-638 |
CD |
denotes |
3 |
T10582 |
638-639 |
SYM |
denotes |
' |
T10583 |
639-640 |
-RRB- |
denotes |
) |
T10584 |
641-644 |
CC |
denotes |
and |
T10585 |
645-651 |
NN |
denotes |
BB617R |
T10586 |
652-653 |
-LRB- |
denotes |
( |
T10587 |
656-678 |
NN |
denotes |
gtatcttgaactgttggttctg |
T10588 |
653-654 |
CD |
denotes |
5 |
T10589 |
654-655 |
SYM |
denotes |
' |
T10590 |
655-656 |
HYPH |
denotes |
- |
T10591 |
678-679 |
HYPH |
denotes |
- |
T10592 |
679-680 |
CD |
denotes |
3 |
T10593 |
680-681 |
SYM |
denotes |
' |
T10594 |
681-682 |
-RRB- |
denotes |
) |
T10595 |
682-683 |
. |
denotes |
. |
T10596 |
683-890 |
sentence |
denotes |
For analysis of TACC1 splice variants, the forward primers used were located in exon 1b: EF (5'-gagagatgcgaaatcagcg-3'), Exon 1d: X3F (5'-agtcaaagaaggcatctgcag-3'), Exon 1a: 1DF (5'-ccaagttctgcgccatggg-3'). |
T10597 |
684-687 |
IN |
denotes |
For |
T10598 |
753-760 |
VBN |
denotes |
located |
T10599 |
688-696 |
NN |
denotes |
analysis |
T10600 |
697-699 |
IN |
denotes |
of |
T10601 |
700-705 |
NN |
denotes |
TACC1 |
T10602 |
713-721 |
NNS |
denotes |
variants |
T10603 |
706-712 |
NN |
denotes |
splice |
T10604 |
721-723 |
, |
denotes |
, |
T10605 |
723-726 |
DT |
denotes |
the |
T10606 |
735-742 |
NNS |
denotes |
primers |
T10607 |
727-734 |
JJ |
denotes |
forward |
T10608 |
743-747 |
VBN |
denotes |
used |
T10609 |
748-752 |
VBD |
denotes |
were |
T10610 |
761-763 |
IN |
denotes |
in |
T10611 |
764-768 |
NN |
denotes |
exon |
T10612 |
769-771 |
CD |
denotes |
1b |
T10613 |
771-773 |
: |
denotes |
: |
T10614 |
773-775 |
NN |
denotes |
EF |
T10615 |
780-799 |
NN |
denotes |
gagagatgcgaaatcagcg |
T10616 |
776-777 |
-LRB- |
denotes |
( |
T10617 |
777-778 |
CD |
denotes |
5 |
T10618 |
778-779 |
SYM |
denotes |
' |
T10619 |
779-780 |
HYPH |
denotes |
- |
T10620 |
799-800 |
HYPH |
denotes |
- |
T10621 |
800-801 |
CD |
denotes |
3 |
T10622 |
801-802 |
SYM |
denotes |
' |
T10623 |
802-803 |
-RRB- |
denotes |
) |
T10624 |
803-805 |
, |
denotes |
, |
T10625 |
805-809 |
NN |
denotes |
Exon |
T10626 |
810-812 |
CD |
denotes |
1d |
T10627 |
812-814 |
: |
denotes |
: |
T10628 |
814-817 |
NN |
denotes |
X3F |
T10629 |
822-843 |
NN |
denotes |
agtcaaagaaggcatctgcag |
T10630 |
818-819 |
-LRB- |
denotes |
( |
T10631 |
819-820 |
CD |
denotes |
5 |
T10632 |
820-821 |
SYM |
denotes |
' |
T10633 |
821-822 |
HYPH |
denotes |
- |
T10634 |
843-844 |
HYPH |
denotes |
- |
T10635 |
844-845 |
CD |
denotes |
3 |
T10636 |
845-846 |
SYM |
denotes |
' |
T10637 |
846-847 |
-RRB- |
denotes |
) |
T10638 |
847-849 |
, |
denotes |
, |
T10639 |
849-853 |
NN |
denotes |
Exon |
T10640 |
854-856 |
CD |
denotes |
1a |
T10641 |
856-858 |
: |
denotes |
: |
T10642 |
858-861 |
NN |
denotes |
1DF |
T10643 |
866-885 |
NN |
denotes |
ccaagttctgcgccatggg |
T10644 |
862-863 |
-LRB- |
denotes |
( |
T10645 |
863-864 |
CD |
denotes |
5 |
T10646 |
864-865 |
SYM |
denotes |
' |
T10647 |
865-866 |
HYPH |
denotes |
- |
T10648 |
885-886 |
HYPH |
denotes |
- |
T10649 |
886-887 |
CD |
denotes |
3 |
T10650 |
887-888 |
SYM |
denotes |
' |
T10651 |
888-889 |
-RRB- |
denotes |
) |
T10652 |
889-890 |
. |
denotes |
. |
T10653 |
890-1149 |
sentence |
denotes |
The reverse primers used were: Exon 1: X1R (5'-ggatttggtctcggcttgcgaatc-3'), Exon 2: BX647R (5'-cttgtgattcttggcttttgg-3'), Exon 3: 6SPR (5'-gtcatcgcctcgtcctggagggc-3'), Exon 4a: 1DR (5'-aatttcacttgttcagtagtc-3'), Exon 5: 128R (5'-cctgcttctgaggatgaaaacgc-3'). |
T10654 |
891-894 |
DT |
denotes |
The |
T10655 |
903-910 |
NNS |
denotes |
primers |
T10656 |
895-902 |
JJ |
denotes |
reverse |
T10657 |
916-920 |
VBD |
denotes |
were |
T10658 |
911-915 |
VBN |
denotes |
used |
T10659 |
920-922 |
: |
denotes |
: |
T10660 |
922-926 |
NN |
denotes |
Exon |
T10661 |
927-928 |
CD |
denotes |
1 |
T10662 |
928-930 |
: |
denotes |
: |
T10663 |
930-933 |
NN |
denotes |
X1R |
T10664 |
938-962 |
NN |
denotes |
ggatttggtctcggcttgcgaatc |
T10665 |
934-935 |
-LRB- |
denotes |
( |
T10666 |
935-936 |
CD |
denotes |
5 |
T10667 |
936-937 |
SYM |
denotes |
' |
T10668 |
937-938 |
HYPH |
denotes |
- |
T10669 |
962-963 |
HYPH |
denotes |
- |
T10670 |
963-964 |
CD |
denotes |
3 |
T10671 |
964-965 |
SYM |
denotes |
' |
T10672 |
965-966 |
-RRB- |
denotes |
) |
T10673 |
966-968 |
, |
denotes |
, |
T10674 |
968-972 |
NN |
denotes |
Exon |
T10675 |
973-974 |
CD |
denotes |
2 |
T10676 |
974-976 |
: |
denotes |
: |
T10677 |
976-982 |
NN |
denotes |
BX647R |
T10678 |
987-1008 |
NN |
denotes |
cttgtgattcttggcttttgg |
T10679 |
983-984 |
-LRB- |
denotes |
( |
T10680 |
984-985 |
CD |
denotes |
5 |
T10681 |
985-986 |
SYM |
denotes |
' |
T10682 |
986-987 |
HYPH |
denotes |
- |
T10683 |
1008-1009 |
HYPH |
denotes |
- |
T10684 |
1009-1010 |
CD |
denotes |
3 |
T10685 |
1010-1011 |
SYM |
denotes |
' |
T10686 |
1011-1012 |
-RRB- |
denotes |
) |
T10687 |
1012-1014 |
, |
denotes |
, |
T10688 |
1014-1018 |
NN |
denotes |
Exon |
T10689 |
1019-1020 |
CD |
denotes |
3 |
T10690 |
1020-1022 |
: |
denotes |
: |
T10691 |
1022-1026 |
NN |
denotes |
6SPR |
T10692 |
1031-1054 |
NN |
denotes |
gtcatcgcctcgtcctggagggc |
T10693 |
1027-1028 |
-LRB- |
denotes |
( |
T10694 |
1028-1029 |
CD |
denotes |
5 |
T10695 |
1029-1030 |
SYM |
denotes |
' |
T10696 |
1030-1031 |
HYPH |
denotes |
- |
T10697 |
1054-1055 |
HYPH |
denotes |
- |
T10698 |
1055-1056 |
CD |
denotes |
3 |
T10699 |
1056-1057 |
SYM |
denotes |
' |
T10700 |
1057-1058 |
-RRB- |
denotes |
) |
T10701 |
1058-1060 |
, |
denotes |
, |
T10702 |
1060-1064 |
NN |
denotes |
Exon |
T10703 |
1065-1067 |
NN |
denotes |
4a |
T10704 |
1067-1069 |
: |
denotes |
: |
T10705 |
1069-1072 |
NN |
denotes |
1DR |
T10706 |
1077-1098 |
NN |
denotes |
aatttcacttgttcagtagtc |
T10707 |
1073-1074 |
-LRB- |
denotes |
( |
T10708 |
1074-1075 |
CD |
denotes |
5 |
T10709 |
1075-1076 |
SYM |
denotes |
' |
T10710 |
1076-1077 |
HYPH |
denotes |
- |
T10711 |
1098-1099 |
HYPH |
denotes |
- |
T10712 |
1099-1100 |
CD |
denotes |
3 |
T10713 |
1100-1101 |
SYM |
denotes |
' |
T10714 |
1101-1102 |
-RRB- |
denotes |
) |
T10715 |
1102-1104 |
, |
denotes |
, |
T10716 |
1104-1108 |
NN |
denotes |
Exon |
T10717 |
1109-1110 |
CD |
denotes |
5 |
T10718 |
1110-1112 |
: |
denotes |
: |
T10719 |
1112-1116 |
NN |
denotes |
128R |
T10720 |
1121-1144 |
NN |
denotes |
cctgcttctgaggatgaaaacgc |
T10721 |
1117-1118 |
-LRB- |
denotes |
( |
T10722 |
1118-1119 |
CD |
denotes |
5 |
T10723 |
1119-1120 |
SYM |
denotes |
' |
T10724 |
1120-1121 |
HYPH |
denotes |
- |
T10725 |
1144-1145 |
HYPH |
denotes |
- |
T10726 |
1145-1146 |
CD |
denotes |
3 |
T10727 |
1146-1147 |
SYM |
denotes |
' |
T10728 |
1147-1148 |
-RRB- |
denotes |
) |
T10729 |
1148-1149 |
. |
denotes |
. |
T10730 |
1149-1282 |
sentence |
denotes |
Rabbit brain poly A+ mRNA, mouse testis and human brain total RNA were obtained from BD Bioscience Clontech (Palo Alto, CA, U.S.A.). |
T10731 |
1150-1156 |
NN |
denotes |
Rabbit |
T10732 |
1157-1162 |
NN |
denotes |
brain |
T10733 |
1171-1175 |
NN |
denotes |
mRNA |
T10734 |
1163-1167 |
NN |
denotes |
poly |
T10735 |
1168-1170 |
NN |
denotes |
A+ |
T10736 |
1221-1229 |
VBN |
denotes |
obtained |
T10737 |
1175-1177 |
, |
denotes |
, |
T10738 |
1177-1182 |
NN |
denotes |
mouse |
T10739 |
1183-1189 |
NN |
denotes |
testis |
T10740 |
1190-1193 |
CC |
denotes |
and |
T10741 |
1194-1199 |
JJ |
denotes |
human |
T10742 |
1200-1205 |
NN |
denotes |
brain |
T10743 |
1212-1215 |
NN |
denotes |
RNA |
T10744 |
1206-1211 |
JJ |
denotes |
total |
T10745 |
1216-1220 |
VBD |
denotes |
were |
T10746 |
1230-1234 |
IN |
denotes |
from |
T10747 |
1235-1237 |
NNP |
denotes |
BD |
T10748 |
1249-1257 |
NNP |
denotes |
Clontech |
T10749 |
1238-1248 |
NNP |
denotes |
Bioscience |
T10750 |
1258-1259 |
-LRB- |
denotes |
( |
T10751 |
1259-1263 |
NNP |
denotes |
Palo |
T10752 |
1264-1268 |
NNP |
denotes |
Alto |
T10753 |
1268-1270 |
, |
denotes |
, |
T10754 |
1270-1272 |
NNP |
denotes |
CA |
T10755 |
1272-1274 |
, |
denotes |
, |
T10756 |
1274-1280 |
NNP |
denotes |
U.S.A. |
T10757 |
1280-1281 |
-RRB- |
denotes |
) |
T10758 |
1281-1282 |
. |
denotes |
. |
T10759 |
1282-1452 |
sentence |
denotes |
Reverse transcription and PCR was performed as previously described, using either 1 μg of total RNA or 50 ng of poly A+ mRNA as template for first strand cDNA synthesis. |
T10760 |
1283-1290 |
JJ |
denotes |
Reverse |
T10761 |
1291-1304 |
NN |
denotes |
transcription |
T10762 |
1317-1326 |
VBN |
denotes |
performed |
T10763 |
1305-1308 |
CC |
denotes |
and |
T10764 |
1309-1312 |
NN |
denotes |
PCR |
T10765 |
1313-1316 |
VBD |
denotes |
was |
T10766 |
1327-1329 |
IN |
denotes |
as |
T10767 |
1341-1350 |
VBN |
denotes |
described |
T10768 |
1330-1340 |
RB |
denotes |
previously |
T10769 |
1350-1352 |
, |
denotes |
, |
T10770 |
1352-1357 |
VBG |
denotes |
using |
T10771 |
1358-1364 |
CC |
denotes |
either |
T10772 |
1367-1369 |
NN |
denotes |
μg |
T10773 |
1365-1366 |
CD |
denotes |
1 |
T10774 |
1370-1372 |
IN |
denotes |
of |
T10775 |
1373-1378 |
JJ |
denotes |
total |
T10776 |
1379-1382 |
NN |
denotes |
RNA |
T10777 |
1383-1385 |
CC |
denotes |
or |
T10778 |
1386-1388 |
CD |
denotes |
50 |
T10779 |
1389-1391 |
NNS |
denotes |
ng |
T10780 |
1392-1394 |
IN |
denotes |
of |
T10781 |
1395-1399 |
NN |
denotes |
poly |
T10782 |
1400-1402 |
NN |
denotes |
A+ |
T10783 |
1403-1407 |
NN |
denotes |
mRNA |
T10784 |
1408-1410 |
IN |
denotes |
as |
T10785 |
1411-1419 |
NN |
denotes |
template |
T10786 |
1420-1423 |
IN |
denotes |
for |
T10787 |
1424-1429 |
JJ |
denotes |
first |
T10788 |
1430-1436 |
NN |
denotes |
strand |
T10789 |
1437-1441 |
NN |
denotes |
cDNA |
T10790 |
1442-1451 |
NN |
denotes |
synthesis |
T10791 |
1451-1452 |
. |
denotes |
. |
T10792 |
1452-1568 |
sentence |
denotes |
PCR products were cloned into pCR2.1 (Invitrogen, Carlsbad CA, U.S.A.) and transformed into InvαF' competent cells. |
T10793 |
1453-1456 |
NN |
denotes |
PCR |
T10794 |
1457-1465 |
NNS |
denotes |
products |
T10795 |
1471-1477 |
VBN |
denotes |
cloned |
T10796 |
1466-1470 |
VBD |
denotes |
were |
T10797 |
1478-1482 |
IN |
denotes |
into |
T10798 |
1483-1489 |
NN |
denotes |
pCR2.1 |
T10799 |
1490-1491 |
-LRB- |
denotes |
( |
T10800 |
1491-1501 |
NNP |
denotes |
Invitrogen |
T10801 |
1501-1503 |
, |
denotes |
, |
T10802 |
1503-1511 |
NNP |
denotes |
Carlsbad |
T10803 |
1512-1514 |
NNP |
denotes |
CA |
T10804 |
1514-1516 |
, |
denotes |
, |
T10805 |
1516-1522 |
NNP |
denotes |
U.S.A. |
T10806 |
1522-1523 |
-RRB- |
denotes |
) |
T10807 |
1524-1527 |
CC |
denotes |
and |
T10808 |
1528-1539 |
VBN |
denotes |
transformed |
T10809 |
1540-1544 |
IN |
denotes |
into |
T10810 |
1545-1550 |
NN |
denotes |
InvαF |
T10811 |
1552-1561 |
JJ |
denotes |
competent |
T10812 |
1550-1551 |
POS |
denotes |
' |
T10813 |
1562-1567 |
NNS |
denotes |
cells |
T10814 |
1567-1568 |
. |
denotes |
. |
T10815 |
1568-1662 |
sentence |
denotes |
Plasmid inserts were sequenced by the Roswell Park Cancer Institute Biopolymer Core Facility. |
T10816 |
1569-1576 |
NN |
denotes |
Plasmid |
T10817 |
1577-1584 |
NNS |
denotes |
inserts |
T10818 |
1590-1599 |
VBN |
denotes |
sequenced |
T10819 |
1585-1589 |
VBD |
denotes |
were |
T10820 |
1600-1602 |
IN |
denotes |
by |
T10821 |
1603-1606 |
DT |
denotes |
the |
T10822 |
1653-1661 |
NNP |
denotes |
Facility |
T10823 |
1607-1614 |
NNP |
denotes |
Roswell |
T10824 |
1615-1619 |
NNP |
denotes |
Park |
T10825 |
1627-1636 |
NNP |
denotes |
Institute |
T10826 |
1620-1626 |
NNP |
denotes |
Cancer |
T10827 |
1637-1647 |
NNP |
denotes |
Biopolymer |
T10828 |
1648-1652 |
NNP |
denotes |
Core |
T10829 |
1661-1662 |
. |
denotes |
. |
T10439 |
0-7 |
NN |
denotes |
Cloning |
R6688 |
T10440 |
T10439 |
prep |
of,Cloning |
R6689 |
T10441 |
T10442 |
compound |
vertebrate,TACC |
R6690 |
T10442 |
T10443 |
compound |
TACC,cDNAs |
R6691 |
T10443 |
T10440 |
pobj |
cDNAs,of |
R6692 |
T10445 |
T10446 |
det |
The,TACC3 |
R6693 |
T10446 |
T10448 |
nsubjpass |
TACC3,cloned |
R6694 |
T10447 |
T10446 |
compound |
rabbit,TACC3 |
R6695 |
T10449 |
T10448 |
auxpass |
was,cloned |
R6696 |
T10450 |
T10448 |
prep |
by,cloned |
R6697 |
T10451 |
T10452 |
compound |
rt,PCR |
R6698 |
T10452 |
T10450 |
pobj |
PCR,by |
R6699 |
T10453 |
T10452 |
punct |
-,PCR |
R6700 |
T10454 |
T10448 |
advcl |
using,cloned |
R6701 |
T10455 |
T10456 |
det |
the,primer |
R6702 |
T10456 |
T10454 |
dobj |
primer,using |
R6703 |
T10457 |
T10458 |
punct |
"""",specific |
R6704 |
T10458 |
T10456 |
amod |
specific,primer |
R6705 |
T10459 |
T10458 |
npadvmod |
TACC4,specific |
R6706 |
T10460 |
T10458 |
punct |
"""",specific |
R6707 |
T10461 |
T10456 |
appos |
T4RACE2,primer |
R6708 |
T10462 |
T10463 |
punct |
[,9 |
R6709 |
T10463 |
T10456 |
parataxis |
9,primer |
R6710 |
T10464 |
T10463 |
punct |
],9 |
R6711 |
T10465 |
T10466 |
punct |
(,cccgaactgctccaggtaatcgatctc |
R6712 |
T10466 |
T10456 |
parataxis |
cccgaactgctccaggtaatcgatctc,primer |
R6713 |
T10467 |
T10466 |
nummod |
5,cccgaactgctccaggtaatcgatctc |
R6714 |
T10468 |
T10467 |
punct |
',5 |
R6715 |
T10469 |
T10466 |
punct |
-,cccgaactgctccaggtaatcgatctc |
R6716 |
T10470 |
T10466 |
punct |
-,cccgaactgctccaggtaatcgatctc |
R6717 |
T10471 |
T10466 |
nummod |
3,cccgaactgctccaggtaatcgatctc |
R6718 |
T10472 |
T10466 |
punct |
',cccgaactgctccaggtaatcgatctc |
R6719 |
T10473 |
T10466 |
punct |
),cccgaactgctccaggtaatcgatctc |
R6720 |
T10474 |
T10456 |
cc |
and,primer |
R6721 |
T10475 |
T10476 |
det |
a,primer |
R6722 |
T10476 |
T10456 |
conj |
primer,primer |
R6723 |
T10477 |
T10476 |
compound |
consensus,primer |
R6724 |
T10478 |
T10476 |
punct |
", ",primer |
R6725 |
T10479 |
T10476 |
appos |
T3con2,primer |
R6726 |
T10480 |
T10476 |
punct |
", ",primer |
R6727 |
T10481 |
T10476 |
acl |
designed,primer |
R6728 |
T10482 |
T10481 |
prep |
to,designed |
R6729 |
T10483 |
T10484 |
det |
the,region |
R6730 |
T10484 |
T10482 |
pobj |
region,to |
R6731 |
T10485 |
T10484 |
acl |
encompassing,region |
R6732 |
T10486 |
T10487 |
det |
the,initiator |
R6733 |
T10487 |
T10485 |
dobj |
initiator,encompassing |
R6734 |
T10488 |
T10487 |
compound |
vertebrate,initiator |
R6735 |
T10489 |
T10487 |
compound |
TACC3,initiator |
R6736 |
T10490 |
T10487 |
appos |
methionine,initiator |
R6737 |
T10491 |
T10492 |
punct |
(,tatgagtctgcaggtcttaaacgac |
R6738 |
T10492 |
T10487 |
parataxis |
tatgagtctgcaggtcttaaacgac,initiator |
R6739 |
T10493 |
T10492 |
nummod |
5,tatgagtctgcaggtcttaaacgac |
R6740 |
T10494 |
T10493 |
punct |
',5 |
R6741 |
T10495 |
T10492 |
punct |
-,tatgagtctgcaggtcttaaacgac |
R6742 |
T10496 |
T10492 |
punct |
-,tatgagtctgcaggtcttaaacgac |
R6743 |
T10497 |
T10492 |
nummod |
3,tatgagtctgcaggtcttaaacgac |
R6744 |
T10498 |
T10492 |
punct |
',tatgagtctgcaggtcttaaacgac |
R6745 |
T10499 |
T10492 |
punct |
),tatgagtctgcaggtcttaaacgac |
R6746 |
T10500 |
T10448 |
punct |
.,cloned |
R6747 |
T10502 |
T10503 |
prep |
For,based |
R6748 |
T10504 |
T10502 |
pcomp |
cloning,For |
R6749 |
T10505 |
T10506 |
det |
the,cDNA |
R6750 |
T10506 |
T10504 |
dobj |
cDNA,cloning |
R6751 |
T10507 |
T10508 |
compound |
mouse,Tacc1X |
R6752 |
T10508 |
T10506 |
compound |
Tacc1X,cDNA |
R6753 |
T10509 |
T10503 |
punct |
", ",based |
R6754 |
T10510 |
T10511 |
det |
the,primers |
R6755 |
T10511 |
T10503 |
nsubjpass |
primers,based |
R6756 |
T10512 |
T10511 |
acl |
used,primers |
R6757 |
T10513 |
T10503 |
auxpass |
were,based |
R6758 |
T10514 |
T10503 |
prep |
upon,based |
R6759 |
T10515 |
T10516 |
det |
the,sequence |
R6760 |
T10516 |
T10514 |
pobj |
sequence,upon |
R6761 |
T10517 |
T10516 |
amod |
genomic,sequence |
R6762 |
T10518 |
T10516 |
acl |
reported,sequence |
R6763 |
T10519 |
T10516 |
punct |
", ",sequence |
R6764 |
T10520 |
T10516 |
cc |
and,sequence |
R6765 |
T10521 |
T10522 |
det |
the,sequence |
R6766 |
T10522 |
T10516 |
conj |
sequence,sequence |
R6767 |
T10523 |
T10522 |
prep |
of,sequence |
R6768 |
T10524 |
T10525 |
det |
the,clone |
R6769 |
T10525 |
T10523 |
pobj |
clone,of |
R6770 |
T10526 |
T10525 |
compound |
IMAGE,clone |
R6771 |
T10527 |
T10525 |
compound |
cDNA,clone |
R6772 |
T10528 |
T10525 |
appos |
4933429K08,clone |
R6773 |
T10529 |
T10522 |
punct |
: ,sequence |
R6774 |
T10530 |
T10522 |
appos |
T1XF,sequence |
R6775 |
T10531 |
T10532 |
punct |
(,ccatgttcagtcattggcaggtc |
R6776 |
T10532 |
T10530 |
parataxis |
ccatgttcagtcattggcaggtc,T1XF |
R6777 |
T10533 |
T10532 |
nummod |
5,ccatgttcagtcattggcaggtc |
R6778 |
T10534 |
T10533 |
punct |
',5 |
R6779 |
T10535 |
T10532 |
punct |
-,ccatgttcagtcattggcaggtc |
R6780 |
T10536 |
T10532 |
punct |
-,ccatgttcagtcattggcaggtc |
R6781 |
T10537 |
T10532 |
nummod |
3,ccatgttcagtcattggcaggtc |
R6782 |
T10538 |
T10532 |
punct |
',ccatgttcagtcattggcaggtc |
R6783 |
T10539 |
T10532 |
punct |
),ccatgttcagtcattggcaggtc |
R6784 |
T10540 |
T10530 |
punct |
", ",T1XF |
R6785 |
T10541 |
T10530 |
conj |
T1XF2,T1XF |
R6786 |
T10542 |
T10543 |
punct |
(,ctgcagaaccaacagttcaag |
R6787 |
T10543 |
T10541 |
parataxis |
ctgcagaaccaacagttcaag,T1XF2 |
R6788 |
T10544 |
T10543 |
nummod |
5,ctgcagaaccaacagttcaag |
R6789 |
T10545 |
T10544 |
punct |
',5 |
R6790 |
T10546 |
T10543 |
punct |
-,ctgcagaaccaacagttcaag |
R6791 |
T10547 |
T10543 |
punct |
-,ctgcagaaccaacagttcaag |
R6792 |
T10548 |
T10543 |
nummod |
3,ctgcagaaccaacagttcaag |
R6793 |
T10549 |
T10543 |
punct |
',ctgcagaaccaacagttcaag |
R6794 |
T10550 |
T10543 |
punct |
),ctgcagaaccaacagttcaag |
R6795 |
T10551 |
T10541 |
punct |
", ",T1XF2 |
R6796 |
T10552 |
T10541 |
conj |
T1XR1,T1XF2 |
R6797 |
T10553 |
T10554 |
punct |
(,agatctgtgacatcacagctc |
R6798 |
T10554 |
T10552 |
parataxis |
agatctgtgacatcacagctc,T1XR1 |
R6799 |
T10555 |
T10554 |
nummod |
5,agatctgtgacatcacagctc |
R6800 |
T10556 |
T10555 |
punct |
',5 |
R6801 |
T10557 |
T10554 |
punct |
-,agatctgtgacatcacagctc |
R6802 |
T10558 |
T10554 |
punct |
-,agatctgtgacatcacagctc |
R6803 |
T10559 |
T10554 |
nummod |
3,agatctgtgacatcacagctc |
R6804 |
T10560 |
T10554 |
punct |
',agatctgtgacatcacagctc |
R6805 |
T10561 |
T10554 |
punct |
),agatctgtgacatcacagctc |
R6806 |
T10562 |
T10552 |
punct |
", ",T1XR1 |
R6807 |
T10563 |
T10552 |
conj |
T1XR2,T1XR1 |
R6808 |
T10564 |
T10565 |
punct |
(,ctcgagtcagttagtcttatccagctt |
R6809 |
T10565 |
T10563 |
parataxis |
ctcgagtcagttagtcttatccagctt,T1XR2 |
R6810 |
T10566 |
T10565 |
nummod |
5,ctcgagtcagttagtcttatccagctt |
R6811 |
T10567 |
T10566 |
punct |
',5 |
R6812 |
T10568 |
T10565 |
punct |
-,ctcgagtcagttagtcttatccagctt |
R6813 |
T10569 |
T10565 |
punct |
-,ctcgagtcagttagtcttatccagctt |
R6814 |
T10570 |
T10565 |
nummod |
3,ctcgagtcagttagtcttatccagctt |
R6815 |
T10571 |
T10565 |
punct |
',ctcgagtcagttagtcttatccagctt |
R6816 |
T10572 |
T10565 |
punct |
),ctcgagtcagttagtcttatccagctt |
R6817 |
T10573 |
T10563 |
punct |
", ",T1XR2 |
R6818 |
T10574 |
T10563 |
conj |
BB617F,T1XR2 |
R6819 |
T10575 |
T10576 |
punct |
(,accaccaacttgagtacctg |
R6820 |
T10576 |
T10574 |
parataxis |
accaccaacttgagtacctg,BB617F |
R6821 |
T10577 |
T10576 |
nummod |
5,accaccaacttgagtacctg |
R6822 |
T10578 |
T10577 |
punct |
',5 |
R6823 |
T10579 |
T10576 |
punct |
-,accaccaacttgagtacctg |
R6824 |
T10580 |
T10576 |
punct |
-,accaccaacttgagtacctg |
R6825 |
T10581 |
T10576 |
nummod |
3,accaccaacttgagtacctg |
R6826 |
T10582 |
T10576 |
punct |
',accaccaacttgagtacctg |
R6827 |
T10583 |
T10576 |
punct |
),accaccaacttgagtacctg |
R6828 |
T10584 |
T10574 |
cc |
and,BB617F |
R6829 |
T10585 |
T10574 |
conj |
BB617R,BB617F |
R6830 |
T10586 |
T10587 |
punct |
(,gtatcttgaactgttggttctg |
R6831 |
T10587 |
T10585 |
parataxis |
gtatcttgaactgttggttctg,BB617R |
R6832 |
T10588 |
T10587 |
nummod |
5,gtatcttgaactgttggttctg |
R6833 |
T10589 |
T10588 |
punct |
',5 |
R6834 |
T10590 |
T10587 |
punct |
-,gtatcttgaactgttggttctg |
R6835 |
T10591 |
T10587 |
punct |
-,gtatcttgaactgttggttctg |
R6836 |
T10592 |
T10587 |
nummod |
3,gtatcttgaactgttggttctg |
R6837 |
T10593 |
T10587 |
punct |
',gtatcttgaactgttggttctg |
R6838 |
T10594 |
T10587 |
punct |
),gtatcttgaactgttggttctg |
R6839 |
T10595 |
T10503 |
punct |
.,based |
R6840 |
T10597 |
T10598 |
prep |
For,located |
R6841 |
T10599 |
T10597 |
pobj |
analysis,For |
R6842 |
T10600 |
T10599 |
prep |
of,analysis |
R6843 |
T10601 |
T10602 |
compound |
TACC1,variants |
R6844 |
T10602 |
T10600 |
pobj |
variants,of |
R6845 |
T10603 |
T10602 |
compound |
splice,variants |
R6846 |
T10604 |
T10598 |
punct |
", ",located |
R6847 |
T10605 |
T10606 |
det |
the,primers |
R6848 |
T10606 |
T10598 |
nsubjpass |
primers,located |
R6849 |
T10607 |
T10606 |
amod |
forward,primers |
R6850 |
T10608 |
T10606 |
acl |
used,primers |
R6851 |
T10609 |
T10598 |
auxpass |
were,located |
R6852 |
T10610 |
T10598 |
prep |
in,located |
R6853 |
T10611 |
T10610 |
pobj |
exon,in |
R6854 |
T10612 |
T10611 |
nummod |
1b,exon |
R6855 |
T10613 |
T10611 |
punct |
: ,exon |
R6856 |
T10614 |
T10615 |
dep |
EF,gagagatgcgaaatcagcg |
R6857 |
T10615 |
T10611 |
parataxis |
gagagatgcgaaatcagcg,exon |
R6858 |
T10616 |
T10615 |
punct |
(,gagagatgcgaaatcagcg |
R6859 |
T10617 |
T10615 |
nummod |
5,gagagatgcgaaatcagcg |
R6860 |
T10618 |
T10617 |
punct |
',5 |
R6861 |
T10619 |
T10615 |
punct |
-,gagagatgcgaaatcagcg |
R6862 |
T10620 |
T10615 |
punct |
-,gagagatgcgaaatcagcg |
R6863 |
T10621 |
T10615 |
nummod |
3,gagagatgcgaaatcagcg |
R6864 |
T10622 |
T10615 |
punct |
',gagagatgcgaaatcagcg |
R6865 |
T10623 |
T10615 |
punct |
),gagagatgcgaaatcagcg |
R6866 |
T10624 |
T10611 |
punct |
", ",exon |
R6867 |
T10625 |
T10611 |
appos |
Exon,exon |
R6868 |
T10626 |
T10625 |
nummod |
1d,Exon |
R6869 |
T10627 |
T10625 |
punct |
: ,Exon |
R6870 |
T10628 |
T10629 |
dep |
X3F,agtcaaagaaggcatctgcag |
R6871 |
T10629 |
T10625 |
parataxis |
agtcaaagaaggcatctgcag,Exon |
R6872 |
T10630 |
T10629 |
punct |
(,agtcaaagaaggcatctgcag |
R6873 |
T10631 |
T10629 |
nummod |
5,agtcaaagaaggcatctgcag |
R6874 |
T10632 |
T10631 |
punct |
',5 |
R6875 |
T10633 |
T10629 |
punct |
-,agtcaaagaaggcatctgcag |
R6876 |
T10634 |
T10629 |
punct |
-,agtcaaagaaggcatctgcag |
R6877 |
T10635 |
T10629 |
nummod |
3,agtcaaagaaggcatctgcag |
R6878 |
T10636 |
T10629 |
punct |
',agtcaaagaaggcatctgcag |
R6879 |
T10637 |
T10629 |
punct |
),agtcaaagaaggcatctgcag |
R6880 |
T10638 |
T10611 |
punct |
", ",exon |
R6881 |
T10639 |
T10611 |
appos |
Exon,exon |
R6882 |
T10640 |
T10639 |
nummod |
1a,Exon |
R6883 |
T10641 |
T10639 |
punct |
: ,Exon |
R6884 |
T10642 |
T10643 |
dep |
1DF,ccaagttctgcgccatggg |
R6885 |
T10643 |
T10639 |
parataxis |
ccaagttctgcgccatggg,Exon |
R6886 |
T10644 |
T10643 |
punct |
(,ccaagttctgcgccatggg |
R6887 |
T10645 |
T10643 |
nummod |
5,ccaagttctgcgccatggg |
R6888 |
T10646 |
T10645 |
punct |
',5 |
R6889 |
T10647 |
T10643 |
punct |
-,ccaagttctgcgccatggg |
R6890 |
T10648 |
T10643 |
punct |
-,ccaagttctgcgccatggg |
R6891 |
T10649 |
T10643 |
nummod |
3,ccaagttctgcgccatggg |
R6892 |
T10650 |
T10643 |
punct |
',ccaagttctgcgccatggg |
R6893 |
T10651 |
T10643 |
punct |
),ccaagttctgcgccatggg |
R6894 |
T10652 |
T10598 |
punct |
.,located |
R6895 |
T10654 |
T10655 |
det |
The,primers |
R6896 |
T10655 |
T10657 |
nsubj |
primers,were |
R6897 |
T10656 |
T10655 |
amod |
reverse,primers |
R6898 |
T10658 |
T10655 |
acl |
used,primers |
R6899 |
T10659 |
T10657 |
punct |
: ,were |
R6900 |
T10660 |
T10657 |
attr |
Exon,were |
R6901 |
T10661 |
T10660 |
nummod |
1,Exon |
R6902 |
T10662 |
T10660 |
punct |
: ,Exon |
R6903 |
T10663 |
T10664 |
dep |
X1R,ggatttggtctcggcttgcgaatc |
R6904 |
T10664 |
T10660 |
parataxis |
ggatttggtctcggcttgcgaatc,Exon |
R6905 |
T10665 |
T10664 |
punct |
(,ggatttggtctcggcttgcgaatc |
R6906 |
T10666 |
T10664 |
nummod |
5,ggatttggtctcggcttgcgaatc |
R6907 |
T10667 |
T10666 |
punct |
',5 |
R6908 |
T10668 |
T10664 |
punct |
-,ggatttggtctcggcttgcgaatc |
R6909 |
T10669 |
T10664 |
punct |
-,ggatttggtctcggcttgcgaatc |
R6910 |
T10670 |
T10664 |
nummod |
3,ggatttggtctcggcttgcgaatc |
R6911 |
T10671 |
T10664 |
punct |
',ggatttggtctcggcttgcgaatc |
R6912 |
T10672 |
T10664 |
punct |
),ggatttggtctcggcttgcgaatc |
R6913 |
T10673 |
T10660 |
punct |
", ",Exon |
R6914 |
T10674 |
T10660 |
appos |
Exon,Exon |
R6915 |
T10675 |
T10674 |
nummod |
2,Exon |
R6916 |
T10676 |
T10674 |
punct |
: ,Exon |
R6917 |
T10677 |
T10678 |
dep |
BX647R,cttgtgattcttggcttttgg |
R6918 |
T10678 |
T10674 |
parataxis |
cttgtgattcttggcttttgg,Exon |
R6919 |
T10679 |
T10678 |
punct |
(,cttgtgattcttggcttttgg |
R6920 |
T10680 |
T10678 |
nummod |
5,cttgtgattcttggcttttgg |
R6921 |
T10681 |
T10680 |
punct |
',5 |
R6922 |
T10682 |
T10678 |
punct |
-,cttgtgattcttggcttttgg |
R6923 |
T10683 |
T10678 |
punct |
-,cttgtgattcttggcttttgg |
R6924 |
T10684 |
T10678 |
nummod |
3,cttgtgattcttggcttttgg |
R6925 |
T10685 |
T10678 |
punct |
',cttgtgattcttggcttttgg |
R6926 |
T10686 |
T10678 |
punct |
),cttgtgattcttggcttttgg |
R6927 |
T10687 |
T10660 |
punct |
", ",Exon |
R6928 |
T10688 |
T10660 |
appos |
Exon,Exon |
R6929 |
T10689 |
T10688 |
nummod |
3,Exon |
R6930 |
T10690 |
T10688 |
punct |
: ,Exon |
R6931 |
T10691 |
T10692 |
dep |
6SPR,gtcatcgcctcgtcctggagggc |
R6932 |
T10692 |
T10688 |
parataxis |
gtcatcgcctcgtcctggagggc,Exon |
R6933 |
T10693 |
T10692 |
punct |
(,gtcatcgcctcgtcctggagggc |
R6934 |
T10694 |
T10692 |
nummod |
5,gtcatcgcctcgtcctggagggc |
R6935 |
T10695 |
T10694 |
punct |
',5 |
R6936 |
T10696 |
T10692 |
punct |
-,gtcatcgcctcgtcctggagggc |
R6937 |
T10697 |
T10692 |
punct |
-,gtcatcgcctcgtcctggagggc |
R6938 |
T10698 |
T10692 |
nummod |
3,gtcatcgcctcgtcctggagggc |
R6939 |
T10699 |
T10692 |
punct |
',gtcatcgcctcgtcctggagggc |
R6940 |
T10700 |
T10692 |
punct |
),gtcatcgcctcgtcctggagggc |
R6941 |
T10701 |
T10660 |
punct |
", ",Exon |
R6942 |
T10702 |
T10703 |
compound |
Exon,4a |
R6943 |
T10703 |
T10660 |
appos |
4a,Exon |
R6944 |
T10704 |
T10703 |
punct |
: ,4a |
R6945 |
T10705 |
T10706 |
dep |
1DR,aatttcacttgttcagtagtc |
R6946 |
T10706 |
T10703 |
parataxis |
aatttcacttgttcagtagtc,4a |
R6947 |
T10707 |
T10706 |
punct |
(,aatttcacttgttcagtagtc |
R6948 |
T10708 |
T10706 |
nummod |
5,aatttcacttgttcagtagtc |
R6949 |
T10709 |
T10708 |
punct |
',5 |
R6950 |
T10710 |
T10706 |
punct |
-,aatttcacttgttcagtagtc |
R6951 |
T10711 |
T10706 |
punct |
-,aatttcacttgttcagtagtc |
R6952 |
T10712 |
T10706 |
nummod |
3,aatttcacttgttcagtagtc |
R6953 |
T10713 |
T10706 |
punct |
',aatttcacttgttcagtagtc |
R6954 |
T10714 |
T10706 |
punct |
),aatttcacttgttcagtagtc |
R6955 |
T10715 |
T10660 |
punct |
", ",Exon |
R6956 |
T10716 |
T10660 |
appos |
Exon,Exon |
R6957 |
T10717 |
T10716 |
nummod |
5,Exon |
R6958 |
T10718 |
T10716 |
punct |
: ,Exon |
R6959 |
T10719 |
T10720 |
dep |
128R,cctgcttctgaggatgaaaacgc |
R6960 |
T10720 |
T10716 |
parataxis |
cctgcttctgaggatgaaaacgc,Exon |
R6961 |
T10721 |
T10720 |
punct |
(,cctgcttctgaggatgaaaacgc |
R6962 |
T10722 |
T10720 |
nummod |
5,cctgcttctgaggatgaaaacgc |
R6963 |
T10723 |
T10722 |
punct |
',5 |
R6964 |
T10724 |
T10720 |
punct |
-,cctgcttctgaggatgaaaacgc |
R6965 |
T10725 |
T10720 |
punct |
-,cctgcttctgaggatgaaaacgc |
R6966 |
T10726 |
T10720 |
nummod |
3,cctgcttctgaggatgaaaacgc |
R6967 |
T10727 |
T10720 |
punct |
',cctgcttctgaggatgaaaacgc |
R6968 |
T10728 |
T10720 |
punct |
),cctgcttctgaggatgaaaacgc |
R6969 |
T10729 |
T10657 |
punct |
.,were |
R6970 |
T10731 |
T10732 |
compound |
Rabbit,brain |
R6971 |
T10732 |
T10733 |
compound |
brain,mRNA |
R6972 |
T10733 |
T10736 |
nsubjpass |
mRNA,obtained |
R6973 |
T10734 |
T10733 |
compound |
poly,mRNA |
R6974 |
T10735 |
T10733 |
compound |
A+,mRNA |
R6975 |
T10737 |
T10733 |
punct |
", ",mRNA |
R6976 |
T10738 |
T10739 |
compound |
mouse,testis |
R6977 |
T10739 |
T10733 |
conj |
testis,mRNA |
R6978 |
T10740 |
T10739 |
cc |
and,testis |
R6979 |
T10741 |
T10742 |
amod |
human,brain |
R6980 |
T10742 |
T10743 |
nmod |
brain,RNA |
R6981 |
T10743 |
T10739 |
conj |
RNA,testis |
R6982 |
T10744 |
T10743 |
amod |
total,RNA |
R6983 |
T10745 |
T10736 |
auxpass |
were,obtained |
R6984 |
T10746 |
T10736 |
prep |
from,obtained |
R6985 |
T10747 |
T10748 |
compound |
BD,Clontech |
R6986 |
T10748 |
T10746 |
pobj |
Clontech,from |
R6987 |
T10749 |
T10748 |
compound |
Bioscience,Clontech |
R6988 |
T10750 |
T10748 |
punct |
(,Clontech |
R6989 |
T10751 |
T10752 |
compound |
Palo,Alto |
R6990 |
T10752 |
T10748 |
appos |
Alto,Clontech |
R6991 |
T10753 |
T10752 |
punct |
", ",Alto |
R6992 |
T10754 |
T10752 |
npadvmod |
CA,Alto |
R6993 |
T10755 |
T10752 |
punct |
", ",Alto |
R6994 |
T10756 |
T10752 |
npadvmod |
U.S.A.,Alto |
R6995 |
T10757 |
T10748 |
punct |
),Clontech |
R6996 |
T10758 |
T10736 |
punct |
.,obtained |
R6997 |
T10760 |
T10761 |
amod |
Reverse,transcription |
R6998 |
T10761 |
T10762 |
nsubjpass |
transcription,performed |
R6999 |
T10763 |
T10761 |
cc |
and,transcription |
R7000 |
T10764 |
T10761 |
conj |
PCR,transcription |
R7001 |
T10765 |
T10762 |
auxpass |
was,performed |
R7002 |
T10766 |
T10767 |
mark |
as,described |
R7003 |
T10767 |
T10762 |
advcl |
described,performed |
R7004 |
T10768 |
T10767 |
advmod |
previously,described |
R7005 |
T10769 |
T10762 |
punct |
", ",performed |
R7006 |
T10770 |
T10762 |
advcl |
using,performed |
R7007 |
T10771 |
T10772 |
preconj |
either,μg |
R7008 |
T10772 |
T10770 |
dobj |
μg,using |
R7009 |
T10773 |
T10772 |
nummod |
1,μg |
R7010 |
T10774 |
T10772 |
prep |
of,μg |
R7011 |
T10775 |
T10776 |
amod |
total,RNA |
R7012 |
T10776 |
T10774 |
pobj |
RNA,of |
R7013 |
T10777 |
T10772 |
cc |
or,μg |
R7014 |
T10778 |
T10779 |
nummod |
50,ng |
R7015 |
T10779 |
T10772 |
conj |
ng,μg |
R7016 |
T10780 |
T10779 |
prep |
of,ng |
R7017 |
T10781 |
T10782 |
compound |
poly,A+ |
R7018 |
T10782 |
T10783 |
compound |
A+,mRNA |
R7019 |
T10783 |
T10780 |
pobj |
mRNA,of |
R7020 |
T10784 |
T10770 |
prep |
as,using |
R7021 |
T10785 |
T10784 |
pobj |
template,as |
R7022 |
T10786 |
T10785 |
prep |
for,template |
R7023 |
T10787 |
T10788 |
amod |
first,strand |
R7024 |
T10788 |
T10789 |
compound |
strand,cDNA |
R7025 |
T10789 |
T10790 |
compound |
cDNA,synthesis |
R7026 |
T10790 |
T10786 |
pobj |
synthesis,for |
R7027 |
T10791 |
T10762 |
punct |
.,performed |
R7028 |
T10793 |
T10794 |
compound |
PCR,products |
R7029 |
T10794 |
T10795 |
nsubjpass |
products,cloned |
R7030 |
T10796 |
T10795 |
auxpass |
were,cloned |
R7031 |
T10797 |
T10795 |
prep |
into,cloned |
R7032 |
T10798 |
T10797 |
pobj |
pCR2.1,into |
R7033 |
T10799 |
T10800 |
punct |
(,Invitrogen |
R7034 |
T10800 |
T10798 |
parataxis |
Invitrogen,pCR2.1 |
R7035 |
T10801 |
T10800 |
punct |
", ",Invitrogen |
R7036 |
T10802 |
T10800 |
npadvmod |
Carlsbad,Invitrogen |
R7037 |
T10803 |
T10800 |
npadvmod |
CA,Invitrogen |
R7038 |
T10804 |
T10800 |
punct |
", ",Invitrogen |
R7039 |
T10805 |
T10800 |
npadvmod |
U.S.A.,Invitrogen |
R7040 |
T10806 |
T10800 |
punct |
),Invitrogen |
R7041 |
T10807 |
T10795 |
cc |
and,cloned |
R7042 |
T10808 |
T10795 |
conj |
transformed,cloned |
R7043 |
T10809 |
T10808 |
prep |
into,transformed |
R7044 |
T10810 |
T10811 |
poss |
InvαF,competent |
R7045 |
T10811 |
T10813 |
amod |
competent,cells |
R7046 |
T10812 |
T10810 |
case |
',InvαF |
R7047 |
T10813 |
T10809 |
pobj |
cells,into |
R7048 |
T10814 |
T10795 |
punct |
.,cloned |
R7049 |
T10816 |
T10817 |
compound |
Plasmid,inserts |
R7050 |
T10817 |
T10818 |
nsubjpass |
inserts,sequenced |
R7051 |
T10819 |
T10818 |
auxpass |
were,sequenced |
R7052 |
T10820 |
T10818 |
agent |
by,sequenced |
R7053 |
T10821 |
T10822 |
det |
the,Facility |
R7054 |
T10822 |
T10820 |
pobj |
Facility,by |
R7055 |
T10823 |
T10824 |
compound |
Roswell,Park |
R7056 |
T10824 |
T10825 |
compound |
Park,Institute |
R7057 |
T10825 |
T10822 |
compound |
Institute,Facility |
R7058 |
T10826 |
T10825 |
compound |
Cancer,Institute |
R7059 |
T10827 |
T10828 |
compound |
Biopolymer,Core |
R7060 |
T10828 |
T10822 |
compound |
Core,Facility |
R7061 |
T10829 |
T10818 |
punct |
.,sequenced |