PMC:4385186 / 5612-6212 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4385186","sourcedb":"PMC","sourceid":"4385186","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4385186","text":"For WGS, genomic DNAs extracted from 14 tumors and matched normal tissues were randomly fragmented and purified. Standard paired-end adaptors were ligated according to the manufacturer’s (Illumina) protocol. Adaptor-ligated fragments were purified with preparatory gel electrophoresis, and identical bands were excised, resulting in two libraries per sample with inserts averaging 500 bp. Four lanes of each of the resulting WGS libraries were subjected to WGS on an Illumina HiSeq 2000. Target depth (65× for tumors and normal samples) and at least 30× haploid coverage were achieved in all samples.","tracks":[]}