Figure 4  AJUBA Acts as a Tumor Suppressor in ESCC
(A) Immunoblotting validation of truncated AJUBA proteins in two identified ESCC tumors.
(B–F) The effect of wild-type and altered AJUBA on cell growth, cell migration, and cell invasion, as monitored by MTT assay (C), colony-formation assay (D), and cell-migration and cell-invasion assays in an xCELLigence RTCA DP system (E and F). The presence of wild-type AJUBA or AJUBA altered by p.Gln353∗ (p.Q353X) or p.Val264fs∗ (p.V264fs) in KYSE30 and KYSE150 cells was confirmed by immunoblotting with anti-AJUBA antibody, which recognizes the N terminus of AJUBA (B). These cell lines all have a low level of endogenous AJUBA.
Data represent the mean ± SD; three independent experiments were performed in triplicate. Statistical analysis was performed with a two-sided t test. ∗∗p < 0.01, ∗p < 0.05.