PMC:4385186 / 14037-15197 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4385186","sourcedb":"PMC","sourceid":"4385186","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4385186","text":"Tumor and matched normal tissues of ESCC individuals were cut into pieces in PBS, swollen in 65 mmol/l KCl for 5 min at 37°C, fixed in cold acetic acid and methanol for 5 min at 4°C, dropped onto slides, and dried at room temperature. For interphase fluorescence in situ hybridization (FISH) analysis, slides were stained with Cytocell enumeration probes against chromosomal region 5q, CBX8 (chr17: 77,768,175–77,770,915), or CBX4 (MIM 603079; chr17: 77,806,954–77,813,213). These probes were conjugated with fluorescein isothiocyanate (FITC) or Cy3.5 (Rainbow Scientific). Probes against chromosomal region 5q or TMC8 (MIM 605829; located near the CBX4 and CBX8 regions) were used as controls for verification of focal CNAs of CBX4 or CBX8. Staining was carried out according to the manufacturer’s protocol. FISH samples were viewed with a fully automated, upright Zeiss Axio-ImagerZ.1 microscope with a 20× objective and DAPI, FITC, and Rhodamine filter cubes. Images were produced with the AxioCam MRm CCD camera and the Axiovision v.4.5 software suite. p values were calculated with a two-sample test for equality of proportions with continuity correction.","tracks":[]}