Figure 4  GDI enables extremely long-term retention of the activation state of Rho GTPases. Simulation of Rho activation dynamics at K mGAP/GDI = 0.01, 01, and 1.0 μM in the presence of various free GDI concentrations (0–2.4 μM) in the GDI-integrated model. A) 600 min after stimulation. B) 12,000 min after stimulation. The activation levels of GTPases were expressed as the concentration of GTP-Rho/Effector complex.