Prolongation of Rho activation in the GDI-integrated model is dependent on K mGAP/GDI and the GAP concentration. Rho activation dynamics were simulated at various K mGEF/GDI values (A), K mGAP/GDI values (B), GEF concentration (C), and GAP concentrations (D) in the GDI-integrated model. The activation levels of GTPases were expressed as the concentration of GTP-Rho/Effector complex.