Gene Enzyme Essentiality Subsystem MG YE MA-133-4 Ribose-5-phosphate isomerase E E Carbohydrate metabolism MA-090-485 UTP-glucose-1-phosphate uridylyltransferase E E MA-101-200 Mannose-6-phosphate isomerase E E MA-120-141 Phosphoacetylglucosamine mutase E E MA-184-380 Acetyl-CoA carboxylase E E MA-072-196 Argininosuccinate lyase E NE Amino acid metabolism MA-213-50 Adenylosuccinate synthase E E MA-213-539 Adenylosuccinate lyase E E MA-326-6 Aspartate carbamoyltransferase E E MA-334-434 Phosphoribosylanthranilate isomerase E NE MA-120-148 2-Acetolactate methylmutase E NE MA-090-434 Dihydroxy-acid dehydratase E NE MA-139-331 Homoaconitate hydratase E NE MA-323-77 Saccharopine dehydrogenase E NE MA-326-106 Ornithine carbamoyltransferase E NE MA-101-393 Imidazole-4-carboxamide isomerase E NE MA-184-558 Imidazoleglycerol-phosphate dehydratase E NE MA-090-452 Glutamine amidotransferase:cyclase E NE MA-320-159 3-dehydroquinate synthase E NE MA-139-157 Chorismate synthase E NE MA-073-62 3-deoxy-7-phosphoheptulonate synthase E NE MA-213-547 Tryptophan synthase E NE MA-297-40 Chorismate mutase E NE MA-120-157 Thioredoxin reductase E E MA-173-30 Purine-nucleoside phosphorylase E E Nucleotide metabolism MA-213-65 IMP dehydrogenase E E MA-120-261 Nucleoside diphosphate kinase E E MA-139-347 Guanylate kinase E E MA-120-138 Ribonucleoside-diphosphate reductase E E MA-055-211 Ribonucleoside-diphosphate reductase E E MA-323-58 Ribonucleoside-diphosphate reductase E E MA-334-356 Thymidylate synthase E E MA-153-455 3(2),5-bisphosphate nucleotidase E NE MA-111-23 phosphoadenylyl-sulfate reductase E NE Energy metabolism MA-182-360 6,7-dimethyl-8-ribityllumazine synthase E E Cofactors and vitamins MA-184-368 Riboflavin synthase E E MA-210-311 Aspartate dehydrogenase E E MA-055-340 Fatty-acyl-CoA synthase E E Lipid metabolism MA-162-131 Palmitoyl-protein thioesterase E E MA-334-239 Very-long-chain enoyl-CoA reductase E E