Consistent with previous studies,2,70 the diversity of ancestry profiles of 23andMe African Americans reveal that individuals comprise the full range from 0% to 100% African ancestry, but, further, that there are differences in estimates of ancestry proportions among regions. Namely, we find differences between states that were slave-holding and those that were “free” at the time of the US Civil War. Reflected in these ancestry patterns are migration routes, such as the trans-Atlantic slave trade that brought Africans through important Southern seaports (as documented online in American FactFinder and American Community Survey Summary File). The small sample sizes from some areas of the US, including parts of the Midwest and Mountain regions, reflects the lower population density of African Americans residing in these regions (see the “The Black Populations” Census Brief).