(D) Air-dried tracheal airway epithelia from wild-type and Ccdc151Snbl mutant mice costained for acetylated α-tubulin (green) and the ODA component DNAH5 (red) were visualized by immunofluorescence microscopy. Nuclei were stained with DAPI (blue). In control mice, DNAH5 localized to the axonemes stained with acetylated α-tubulin (top panels), but in Ccdc151Snbl mutant airway epithelia, DNAH5 is undetectable in the ciliary axonemes (bottom panels) Scale bars represent 10 μm.