PMC:4157146 / 10531-10955 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4157146","sourcedb":"PMC","sourceid":"4157146","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4157146","text":"Zebrafish Microinjections\nIn vitro transcribed mRNAs were generated from linearized plasmid templates using the mMessage mMachine SP6 transcription kit (Ambion #AM1340) from pCS2-ccdc151 plasmid. Translation-blocking morpholinos for ccdc151 (5′-AGACCGACGTGCCGGGCATTATATA-3′) were designed by Gene Tools, and mRNAs were diluted in 10 mg/ml Phenol Red and injected in 500 pl drops into the yolks of 1- to 4-cell-stage embryos.","divisions":[{"label":"title","span":{"begin":0,"end":25}}],"tracks":[]}