Figure 3  Subcellular Localization of Wild-Type and Variant POC1B in hTERT-RPE1 Cells
Localizations of wild-type mRFP-POC1B (A), mRFP-POC1B-p.Gln67del (B), and mRFP-POC1B-p.Arg106Pro (C) (all in red). Additional images are shown in Figure S2. Cilia were counterstained with the basal body and cilium marker GT335 (green) and transition-zone marker RPGRIP1L (cyan). Wild-type mRFP-POC1B showed cytosolic localization with enrichment at the basal body region, as seen in the magnifications in the insets. Both variants showed similar cytosolic localization but lacked enrichment at the base of the cilium. In all pictures, nuclei were stained with DAPI (blue). Scale bars represent 10 μm.