Poc1b morphants display the typical ciliopathy phenotypes previously described by Pearson et al.,15 including pericardial edema, small eyes, pigment mislocalization, and a shortened and curved body axis (Figure S4A). Small eyes were already observed in larvae treated with 2 ng MO and became more frequent as the dose increased (Figure 4B; Figure S4B). At a dose of 6 ng MO, smaller eyes occurred in 39.8% of morphants and were on average significantly smaller than wild-type eyes (Figure 4B; Figure S4B), and 92.5% of morphants displayed one or more of the phenotypes described above without higher mortality than in controls. Specificity of the MO knockdown was previously verified with a second MO targeting the 5′ splice site of poc1b exon 2, which resulted in 59.1% of morphants with small eyes (Figure S4B).