cDNAs encoding human wild-type and variant (p.Gln67del and p.Arg106Pro) proteome of centriole 1B (POC1B, previously Pix1) were cloned in pCS2+/DEST with the use of Gateway Technology (Life Technologies), linearized, and used as templates for in vitro transcription. mRNAs were prepared with the mMESSAGE mMACHINE Kit (Life Technologies) according to the manufacturer’s instructions. A dose of 100 pg mRNA was injected with the MOs as described above.