LCLs were seeded at a density of 550,000 cells/ml 24 hr before nucleofection. Amaxa’s Cell Line 96-well Nucleofector Kit SF (Lonza) was used to perform the transfection. Cells were centrifuged at 90 × g for 10 min at room temperature and resuspended at a concentration of 1,000,000 cells in 20 μl of SF/supplement solution (included in SF Kit Lonza Catalog #V4SC2096) and 2 μM final concentration of AllStars negative Control siRNA labeled with Alexa Fluor 488 (QIAGEN) or a pool of siRNA (QIAGEN) (Table S1). The cells were nucleofected using Amaxa’s DN-100 program. Cells were allowed to rest for 10 min before the addition of prewarmed (in 37°C water bath for a minimum of 20 min) RPMI media and then another 5 min after the addition of warm RPMI media. Cells were then plated for protein harvest. Cells were harvested at 24 and 48 hr postnucleofection for protein measurement by MWAs. Protein levels were quantitated as above with three technical replicates per individual per time point and normalized within an individual across time points to the relative β-actin protein levels. Percentage knockdown was then calculated by dividing the relative targeted protein levels in the targeted siRNA sample by those in the scrambled siRNA control sample for each time point. A knockdown was declared significant if protein levels were reduced after knockdown by greater than two times the percentage standard error (p < 0.05).