(A) Venn diagram showing the overview of 151 genes resulting from a DAPPLE analysis of 336 unique genes. A similar diagram of DAPPLE input genes is shown in Figure S5. For the DAPPLE analysis, we compiled the following lists of genes: (1) 113 genes identified from our de novo CNVs by NETBAG; (2) 122 genes with de novo LoF SNVs from four published exome sequencing studies;7–10 (3) 31 genes with hemizygous LoF SNVs on the X chromosome of male ASD subjects and not observed in male control subjects;16 and (4) 92 ASD-implicated genes previously described as autosomal dominant, X-linked dominant, or X-linked recessive in males.18