Target gene Primer sequence Length of amplicon GenBank accession no. Abcg5 Forward: 5'-AGGTCATGATGCTAGATGAGC-3' 260 bp AH011511 Reverse: 5'-CAAAGGGATTGGAATGTTCAG-3' Abcg8 Forward: 5'-CTCCTCGGAAAGTGACAACAG-3' 190 bp AH011518 Reverse: 5'-TAGATTTCGGATGCCCAGCTC-3' Hmgr Forward: 5'-CCGGCAACAACAAGATCTGTG-3' 114 bp BB664708 Reverse: 5'-ATGTACAGGATGGCGATGCA-3' Cyp7a1 Forward: 5'-CAGGGAGATGCTCTGTGTTCA-3' 121 bp NM_007824 Reverse: 5'-AGGCATACATCCCTTCCGTGA-3' Abca1 Forward: 5'-CCCAGAGCAAAAAGCGACTC-3' 89 bp NM_013454 Reverse: 5'-GGTCATCATCACTTTGGTCCTTG-3' Mdr2 (Abcb4) Forward: 5'-GCAGCGAGAAACGGAACAG-3' 64 bp NM_008830 Reverse: 5'-GGTTGCTGATGCTGCCTAGTT-3' LXRα Forward: 5'-GCTCTGCTCATTGCCATCAG-3' 79 bp AF085745 Reverse: 5'-TGTTGCAGCCTCTCTACTTGGA-3' Srebp-1c Forward: 5'-GGAGCCATGGATTGCACATT-3' 103 bp BI656094 Reverse: 5'-CCTGTCTCACCCCCAGCATA-3' Srebp-2 Forward: 5'-CTGCAGCCTCAAGTGCAAAG-3' 119 bp AF374267 Reverse: 5'-CAGTGTGCCATTGGCTGTCT-3' Cyclophilin Forward: 5'-AAGTTCCATCGTGTCATCAAGGAC-3' 173 bp M60456 Reverse: 5'-CCATTGGTGTCTTTGCCTGC-3'