(D–F) Localizations of GFP-kaptin, GFP-kaptin1–258, and GFP-kaptinp.Met241_Gln246dup (green in merges) in COS-7 cells counterstained with phalloidin (red in merged images). Note that whereas wild-type kaptin was distributed in the cytosol and accumulated at F-actin-rich lamellipodia (D), both alterations showed accumulations at perinuclear regions (E and F).