Kaptin Immunolocalization Studies
(A and B) Flag-kaptin colocalized with F-actin-rich foci at the cell body and in growth cones (examples of both are marked by arrow heads in A) in DIV5 rat hippocampal neurons transfected at DIV3. For clarity, the anti-MAP2 immunostaining was omitted from the merged images. Scale bars represent 10 μm.
(C and D) Endogenous kaptin immunostained together with the presynaptic marker bassoon at DIV14 (C) and with the postsynaptic marker Shank2 at DIV24 (D). Puncta enriched with anti-kaptin immunoreactivity (marked by arrow heads) were rich in F-actin, as shown by fluorescently labeled phalloidin. (C) Furthermore, they were usually contacted by presynapses. The scale bar represents 5 μm. (D) Postsynapses marked by Shank2 were largely positive for both F-actin and anti-kaptin immunolabeling (arrow heads). For clarity, the anti-F-actin staining was omitted from the merged image in (D). High-magnification images are shown. The scale bar represents 2.5 μm.