In conclusion, our results suggest that neuritin13–21, neuritin121–129 and neuritin4–12 might be capable of inducing HLA-A2.1-restricted CD8+ CTL, which would be lethal for neuritin and HLA-A2.1 positive cells. Therefore, identification of neuritin peptide would contribute to the design of epitope-based vaccine for astrocytomas immunotherapy.