10.1371/journal.pone.0061634.g003 Figure 3 Effects of PROP pre-incubation on 5-HT modulated parameters in mussel haemocytes incubated in vitro. (A) cAMP content; (B) relative PKA activities; (C) relative ABCB mRNA expression; (D) relative 5-HT1 mRNA expression. All biological endpoints were assessed after 1 h of exposure to 1 µM 5-HT in the presence/absence of a 15-min pre-incubation with 100 µM PROP. Data are derived from three independent experiments (mean ± SEM); *p<0.05 between pairs of samples. Basal PKA activity  =  1.12±0.17 nmol/min/mg protein.