10.1371/journal.pone.0061634.g006 Figure 6  Effects of H89 pre-incubation on dbcAMP induced stimulation of PKA activity and ABCB mRNA expression in mussel haemocytes incubated in vitro.
(A) relative PKA activities; (B) relative ABCB mRNA expression. Both biological endpoints were assessed after 4 h of exposure to 100 µM dbcAMP in the presence/absence of a 30-min pre-incubation with 10 µM H89. Data are derived from three independent experiments (mean±SEM); *p<0.05 between pairs of samples. Basal PKA activity = 1.12±0.17 nmol/min/mg protein.