PMC:3589482 / 14837-15779
Annnotations
bionlp-st-ge-2016-spacy-parsed
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T8253 | 941-942 | . | denotes | . |
T8252 | 940-941 | -RRB- | denotes | ) |
T8251 | 934-940 | JJ | denotes | sc-158 |
T8250 | 933-934 | -LRB- | denotes | ( |
T8249 | 931-932 | NN | denotes | ε |
T8248 | 930-931 | : | denotes | - |
T8247 | 920-930 | JJ | denotes | anti-C/EBP |
T8246 | 916-919 | CC | denotes | and |
T8245 | 914-915 | -RRB- | denotes | ) |
T8244 | 908-914 | JJ | denotes | sc-151 |
T8243 | 907-908 | -LRB- | denotes | ( |
T8242 | 905-906 | NN | denotes | δ |
T8241 | 904-905 | : | denotes | - |
T8240 | 894-904 | JJ | denotes | anti-C/EBP |
T8239 | 892-893 | , | denotes | , |
T8238 | 891-892 | -RRB- | denotes | ) |
T8237 | 885-891 | JJ | denotes | sc-150 |
T8236 | 884-885 | -LRB- | denotes | ( |
T8235 | 882-883 | NN | denotes | β |
T8234 | 881-882 | : | denotes | - |
T8233 | 871-881 | JJ | denotes | anti-C/EBP |
T8232 | 869-870 | , | denotes | , |
T8231 | 868-869 | -RRB- | denotes | ) |
T8230 | 863-868 | JJ | denotes | sc-61 |
T8229 | 862-863 | -LRB- | denotes | ( |
T8228 | 860-861 | NN | denotes | α |
T8227 | 859-860 | : | denotes | - |
T8226 | 849-859 | JJ | denotes | anti-C/EBP |
T8225 | 847-848 | : | denotes | : |
T8224 | 843-847 | NNP | denotes | Cruz |
T8223 | 837-842 | NNP | denotes | Santa |
T8222 | 832-836 | IN | denotes | from |
T8221 | 822-831 | VBN | denotes | purchased |
T8220 | 817-821 | VBD | denotes | were |
T8219 | 806-816 | NNS | denotes | Antibodies |
T8218 | 804-805 | . | denotes | . |
T8217 | 796-804 | NN | denotes | antibody |
T8216 | 790-795 | NNP | denotes | C/EBP |
T8215 | 781-789 | JJ | denotes | specific |
T8214 | 778-780 | IN | denotes | of |
T8213 | 775-777 | NN | denotes | µg |
T8212 | 773-774 | CD | denotes | 4 |
T8211 | 768-772 | IN | denotes | with |
T8210 | 764-767 | NN | denotes | ice |
T8209 | 761-763 | IN | denotes | on |
T8208 | 757-760 | NNS | denotes | hrs |
T8207 | 755-756 | CD | denotes | 2 |
T8206 | 741-754 | JJ | denotes | pre-incubated |
T8205 | 736-740 | VBD | denotes | were |
T8204 | 727-735 | NNS | denotes | extracts |
T8203 | 719-726 | JJ | denotes | nuclear |
T8202 | 717-718 | , | denotes | , |
T8201 | 711-717 | NNS | denotes | assays |
T8200 | 700-710 | JJ | denotes | supershift |
T8199 | 696-699 | IN | denotes | For |
T8198 | 694-695 | . | denotes | . |
T8197 | 688-694 | NNP | denotes | 10V/cm |
T8196 | 685-687 | IN | denotes | at |
T8195 | 681-684 | NNP | denotes | TBE |
T8194 | 678-680 | CD | denotes | 1x |
T8193 | 675-677 | IN | denotes | in |
T8192 | 673-674 | -RRB- | denotes | ) |
T8191 | 672-673 | -RRB- | denotes | ) |
T8190 | 665-672 | NNP | denotes | Bio-Rad |
T8189 | 664-665 | -LRB- | denotes | ( |
T8188 | 649-663 | NN | denotes | bis-acrylamide |
T8187 | 648-649 | : | denotes | : |
T8186 | 638-648 | NN | denotes | acrylamide |
T8185 | 636-637 | CD | denotes | 1 |
T8184 | 635-636 | NN | denotes | ∶ |
T8183 | 633-635 | CD | denotes | 29 |
T8182 | 632-633 | -LRB- | denotes | ( |
T8181 | 627-631 | NNS | denotes | gels |
T8180 | 612-626 | NN | denotes | polyacrylamide |
T8179 | 610-611 | NN | denotes | % |
T8178 | 609-610 | CD | denotes | 5 |
T8177 | 606-608 | IN | denotes | on |
T8176 | 594-605 | NN | denotes | temperature |
T8175 | 589-593 | NN | denotes | room |
T8174 | 586-588 | IN | denotes | at |
T8173 | 570-585 | NN | denotes | electrophoresis |
T8172 | 567-569 | IN | denotes | by |
T8171 | 558-566 | VBN | denotes | resolved |
T8170 | 553-557 | VBD | denotes | were |
T8169 | 544-552 | NNS | denotes | products |
T8168 | 535-543 | NN | denotes | reaction |
T8167 | 527-534 | VBG | denotes | Binding |
T8166 | 525-526 | . | denotes | . |
T8165 | 514-525 | NN | denotes | temperature |
T8164 | 509-513 | NN | denotes | room |
T8163 | 506-508 | IN | denotes | at |
T8162 | 498-505 | NNS | denotes | minutes |
T8161 | 495-497 | CD | denotes | 20 |
T8160 | 491-494 | IN | denotes | for |
T8159 | 481-490 | VBN | denotes | performed |
T8158 | 476-480 | VBD | denotes | were |
T8157 | 470-475 | NN | denotes | probe |
T8156 | 456-469 | VBN | denotes | radiolabelled |
T8155 | 453-455 | IN | denotes | of |
T8154 | 451-452 | -RRB- | denotes | ) |
T8153 | 449-451 | NN | denotes | ng |
T8152 | 445-448 | CD | denotes | 0.2 |
T8151 | 442-444 | TO | denotes | to |
T8150 | 437-441 | CD | denotes | 0.05 |
T8149 | 436-437 | -LRB- | denotes | ( |
T8148 | 432-435 | NN | denotes | cpm |
T8147 | 425-431 | CD | denotes | 20,000 |
T8146 | 422-424 | TO | denotes | to |
T8145 | 415-421 | CD | denotes | 10,000 |
T8144 | 411-414 | CC | denotes | and |
T8143 | 402-410 | NNS | denotes | extracts |
T8142 | 394-401 | JJ | denotes | nuclear |
T8141 | 391-393 | IN | denotes | of |
T8140 | 388-390 | NN | denotes | µg |
T8139 | 386-387 | CD | denotes | 6 |
T8107 | 282-292 | VBG | denotes | containing |
T8106 | 280-281 | -RRB- | denotes | ) |
T8105 | 278-280 | NN | denotes | µl |
T8104 | 275-277 | CD | denotes | 25 |
T8103 | 274-275 | -LRB- | denotes | ( |
T8102 | 264-273 | NNS | denotes | reactions |
T8101 | 256-263 | JJ | denotes | binding |
T8100 | 252-255 | NNP | denotes | DNA |
T8099 | 250-251 | . | denotes | . |
T8098 | 249-250 | NNP | denotes | ] |
T8097 | 247-249 | CD | denotes | 41 |
T8096 | 246-247 | NNP | denotes | [ |
T8095 | 239-245 | NN | denotes | method |
T8094 | 233-238 | NN | denotes | lysis |
T8093 | 223-232 | NN | denotes | detergent |
T8092 | 219-222 | DT | denotes | the |
T8091 | 216-218 | IN | denotes | by |
T8090 | 207-215 | VBN | denotes | prepared |
T8089 | 202-206 | VBD | denotes | were |
T8088 | 193-201 | NNS | denotes | extracts |
T8087 | 185-192 | JJ | denotes | nuclear |
T8086 | 179-184 | CD | denotes | 264.7 |
T8085 | 175-178 | NNP | denotes | RAW |
T8084 | 173-174 | . | denotes | . |
T8083 | 170-173 | NNP | denotes | ATP |
T8082 | 169-170 | : | denotes | - |
T8081 | 168-169 | NNP | denotes | ] |
T8080 | 163-168 | NNP | denotes | γ-32P |
T8079 | 162-163 | NNP | denotes | [ |
T8078 | 158-161 | CC | denotes | and |
T8077 | 151-157 | NN | denotes | kinase |
T8076 | 136-150 | NN | denotes | polynucleotide |
T8075 | 133-135 | CD | denotes | T4 |
T8074 | 127-132 | VBG | denotes | using |
T8073 | 118-126 | JJ | denotes | prepared |
T8072 | 113-117 | VBD | denotes | were |
T8071 | 106-112 | NNS | denotes | assays |
T8070 | 100-105 | NN | denotes | shift |
T8069 | 96-99 | NN | denotes | gel |
T8068 | 92-95 | IN | denotes | for |
T8067 | 85-91 | NNS | denotes | probes |
T8066 | 69-84 | NN | denotes | oligonucleotide |
T8065 | 53-68 | JJ | denotes | double-stranded |
T8064 | 51-52 | , | denotes | , |
T8063 | 38-51 | NNP | denotes | Radiolabelled |
T8062 | 31-37 | NNP | denotes | Assays |
T8061 | 25-30 | NNP | denotes | Shift |
T8060 | 16-24 | NNP | denotes | Mobility |
T8059 | 0-15 | NNP | denotes | Electrophoretic |
T8138 | 384-385 | , | denotes | , |
T8137 | 383-384 | -RRB- | denotes | ) |
T8136 | 378-383 | JJ | denotes | dI-dC |
T8135 | 377-378 | -LRB- | denotes | ( |
T8134 | 372-376 | NN | denotes | poly |
T8133 | 369-371 | NN | denotes | µg |
T8132 | 367-368 | CD | denotes | 1 |
T8131 | 365-366 | , | denotes | , |
T8130 | 355-365 | NN | denotes | Ficoll-400 |
T8129 | 353-354 | NN | denotes | % |
T8128 | 351-353 | CD | denotes | 20 |
T8127 | 349-350 | , | denotes | , |
T8126 | 341-349 | NN | denotes | glycerol |
T8125 | 339-340 | NN | denotes | % |
T8124 | 337-339 | CD | denotes | 30 |
T8123 | 335-336 | , | denotes | , |
T8122 | 332-335 | NNP | denotes | DTT |
T8121 | 329-331 | NNP | denotes | mM |
T8120 | 327-328 | CD | denotes | 2 |
T8119 | 325-326 | , | denotes | , |
T8118 | 322-325 | NNP | denotes | BSA |
T8117 | 319-321 | NN | denotes | ml |
T8116 | 318-319 | NN | denotes | / |
T8115 | 316-318 | NN | denotes | µg |
T8114 | 313-315 | CD | denotes | 10 |
T8113 | 311-312 | , | denotes | , |
T8112 | 308-311 | CD | denotes | 7.9 |
T8111 | 305-307 | NNP | denotes | pH |
T8110 | 299-304 | NNP | denotes | HEPES |
T8109 | 296-298 | NNP | denotes | mM |
T8108 | 293-295 | CD | denotes | 10 |
R5781 | T8059 | T8063 | compound | Electrophoretic,Radiolabelled |
R5782 | T8060 | T8061 | compound | Mobility,Shift |
R5783 | T8061 | T8063 | compound | Shift,Radiolabelled |
R5784 | T8062 | T8063 | compound | Assays,Radiolabelled |
R5785 | T8063 | T8063 | ROOT | Radiolabelled,Radiolabelled |
R5786 | T8064 | T8063 | punct | ",",Radiolabelled |
R5787 | T8065 | T8067 | amod | double-stranded,probes |
R5788 | T8066 | T8067 | compound | oligonucleotide,probes |
R5789 | T8067 | T8073 | nsubjpass | probes,prepared |
R5790 | T8068 | T8067 | prep | for,probes |
R5791 | T8069 | T8070 | compound | gel,shift |
R5792 | T8070 | T8071 | compound | shift,assays |
R5793 | T8071 | T8068 | pobj | assays,for |
R5794 | T8072 | T8073 | auxpass | were,prepared |
R5795 | T8073 | T8063 | npadvmod | prepared,Radiolabelled |
R5796 | T8074 | T8073 | advcl | using,prepared |
R5797 | T8075 | T8077 | nummod | T4,kinase |
R5798 | T8076 | T8077 | compound | polynucleotide,kinase |
R5799 | T8077 | T8074 | dobj | kinase,using |
R5800 | T8078 | T8077 | cc | and,kinase |
R5801 | T8079 | T8083 | compound | [,ATP |
R5802 | T8080 | T8083 | compound | γ-32P,ATP |
R5803 | T8081 | T8083 | compound | ],ATP |
R5804 | T8082 | T8083 | punct | -,ATP |
R5805 | T8083 | T8077 | conj | ATP,kinase |
R5806 | T8084 | T8073 | punct | .,prepared |
R5807 | T8085 | T8090 | nsubjpass | RAW,prepared |
R5808 | T8086 | T8088 | nummod | 264.7,extracts |
R5809 | T8087 | T8088 | amod | nuclear,extracts |
R5810 | T8088 | T8090 | nsubjpass | extracts,prepared |
R5811 | T8089 | T8090 | auxpass | were,prepared |
R5812 | T8090 | T8090 | ROOT | prepared,prepared |
R5813 | T8091 | T8090 | agent | by,prepared |
R5814 | T8092 | T8095 | det | the,method |
R5815 | T8093 | T8094 | compound | detergent,lysis |
R5816 | T8094 | T8095 | compound | lysis,method |
R5817 | T8095 | T8091 | pobj | method,by |
R5818 | T8096 | T8098 | nmod | [,] |
R5819 | T8097 | T8098 | nummod | 41,] |
R5820 | T8098 | T8095 | appos | ],method |
R5821 | T8099 | T8090 | punct | .,prepared |
R5822 | T8100 | T8159 | nsubjpass | DNA,performed |
R5823 | T8101 | T8102 | amod | binding,reactions |
R5824 | T8102 | T8100 | dobj | reactions,DNA |
R5825 | T8103 | T8102 | punct | (,reactions |
R5826 | T8104 | T8105 | nummod | 25,µl |
R5827 | T8105 | T8102 | appos | µl,reactions |
R5828 | T8106 | T8105 | punct | ),µl |
R5829 | T8107 | T8105 | acl | containing,µl |
R5830 | T8108 | T8111 | nummod | 10,pH |
R5831 | T8109 | T8111 | compound | mM,pH |
R5832 | T8110 | T8111 | compound | HEPES,pH |
R5833 | T8111 | T8107 | dobj | pH,containing |
R5834 | T8112 | T8111 | nummod | 7.9,pH |
R5835 | T8113 | T8111 | punct | ",",pH |
R5836 | T8114 | T8118 | nummod | 10,BSA |
R5837 | T8115 | T8118 | amod | µg,BSA |
R5838 | T8116 | T8118 | nmod | /,BSA |
R5839 | T8117 | T8118 | compound | ml,BSA |
R5840 | T8118 | T8111 | appos | BSA,pH |
R5841 | T8119 | T8111 | punct | ",",pH |
R5842 | T8120 | T8122 | nummod | 2,DTT |
R5843 | T8121 | T8122 | compound | mM,DTT |
R5844 | T8122 | T8111 | conj | DTT,pH |
R5845 | T8123 | T8122 | punct | ",",DTT |
R5846 | T8124 | T8125 | nummod | 30,% |
R5847 | T8125 | T8126 | compound | %,glycerol |
R5848 | T8126 | T8122 | conj | glycerol,DTT |
R5849 | T8127 | T8126 | punct | ",",glycerol |
R5850 | T8128 | T8129 | nummod | 20,% |
R5851 | T8129 | T8130 | compound | %,Ficoll-400 |
R5852 | T8130 | T8126 | appos | Ficoll-400,glycerol |
R5853 | T8131 | T8130 | punct | ",",Ficoll-400 |
R5854 | T8132 | T8134 | nummod | 1,poly |
R5855 | T8133 | T8134 | compound | µg,poly |
R5856 | T8134 | T8159 | nsubjpass | poly,performed |
R5857 | T8135 | T8136 | punct | (,dI-dC |
R5858 | T8136 | T8134 | appos | dI-dC,poly |
R5859 | T8137 | T8136 | punct | ),dI-dC |
R5860 | T8138 | T8134 | punct | ",",poly |
R5861 | T8139 | T8140 | nummod | 6,µg |
R5862 | T8140 | T8134 | appos | µg,poly |
R5863 | T8141 | T8140 | prep | of,µg |
R5864 | T8142 | T8143 | amod | nuclear,extracts |
R5865 | T8143 | T8141 | pobj | extracts,of |
R5866 | T8144 | T8140 | cc | and,µg |
R5867 | T8145 | T8147 | quantmod | "10,000","20,000" |
R5868 | T8146 | T8147 | quantmod | to,"20,000" |
R5869 | T8147 | T8148 | nummod | "20,000",cpm |
R5870 | T8148 | T8140 | conj | cpm,µg |
R5871 | T8149 | T8153 | punct | (,ng |
R5872 | T8150 | T8152 | quantmod | 0.05,0.2 |
R5873 | T8151 | T8152 | quantmod | to,0.2 |
R5874 | T8152 | T8153 | nummod | 0.2,ng |
R5875 | T8153 | T8148 | appos | ng,cpm |
R5876 | T8154 | T8153 | punct | ),ng |
R5877 | T8155 | T8148 | prep | of,cpm |
R5878 | T8156 | T8157 | amod | radiolabelled,probe |
R5879 | T8157 | T8155 | pobj | probe,of |
R5880 | T8158 | T8159 | auxpass | were,performed |
R5881 | T8159 | T8159 | ROOT | performed,performed |
R5882 | T8160 | T8159 | prep | for,performed |
R5883 | T8161 | T8162 | nummod | 20,minutes |
R5884 | T8162 | T8160 | pobj | minutes,for |
R5885 | T8163 | T8162 | prep | at,minutes |
R5886 | T8164 | T8165 | compound | room,temperature |
R5887 | T8165 | T8163 | pobj | temperature,at |
R5888 | T8166 | T8159 | punct | .,performed |
R5889 | T8167 | T8169 | amod | Binding,products |
R5890 | T8168 | T8169 | compound | reaction,products |
R5891 | T8169 | T8171 | nsubjpass | products,resolved |
R5892 | T8170 | T8171 | auxpass | were,resolved |
R5893 | T8171 | T8171 | ROOT | resolved,resolved |
R5894 | T8172 | T8171 | agent | by,resolved |
R5895 | T8173 | T8172 | pobj | electrophoresis,by |
R5896 | T8174 | T8171 | prep | at,resolved |
R5897 | T8175 | T8176 | compound | room,temperature |
R5898 | T8176 | T8174 | pobj | temperature,at |
R5899 | T8177 | T8171 | prep | on,resolved |
R5900 | T8178 | T8179 | nummod | 5,% |
R5901 | T8179 | T8181 | compound | %,gels |
R5902 | T8180 | T8181 | compound | polyacrylamide,gels |
R5903 | T8181 | T8177 | pobj | gels,on |
R5904 | T8182 | T8181 | punct | (,gels |
R5905 | T8183 | T8184 | nummod | 29,∶ |
R5906 | T8184 | T8181 | appos | ∶,gels |
R5907 | T8185 | T8184 | nummod | 1,∶ |
R5908 | T8186 | T8186 | ROOT | acrylamide,acrylamide |
R5909 | T8187 | T8186 | punct | :,acrylamide |
R5910 | T8188 | T8186 | appos | bis-acrylamide,acrylamide |
R5911 | T8189 | T8190 | punct | (,Bio-Rad |
R5912 | T8190 | T8188 | appos | Bio-Rad,bis-acrylamide |
R5913 | T8191 | T8188 | punct | ),bis-acrylamide |
R5914 | T8192 | T8186 | punct | ),acrylamide |
R5915 | T8193 | T8186 | prep | in,acrylamide |
R5916 | T8194 | T8195 | nummod | 1x,TBE |
R5917 | T8195 | T8193 | pobj | TBE,in |
R5918 | T8196 | T8186 | prep | at,acrylamide |
R5919 | T8197 | T8196 | pobj | 10V/cm,at |
R5920 | T8198 | T8186 | punct | .,acrylamide |
R5921 | T8199 | T8205 | prep | For,were |
R5922 | T8200 | T8201 | amod | supershift,assays |
R5923 | T8201 | T8199 | pobj | assays,For |
R5924 | T8202 | T8205 | punct | ",",were |
R5925 | T8203 | T8204 | amod | nuclear,extracts |
R5926 | T8204 | T8205 | nsubj | extracts,were |
R5927 | T8205 | T8205 | ROOT | were,were |
R5928 | T8206 | T8205 | acomp | pre-incubated,were |
R5929 | T8207 | T8208 | nummod | 2,hrs |
R5930 | T8208 | T8206 | dobj | hrs,pre-incubated |
R5931 | T8209 | T8206 | prep | on,pre-incubated |
R5932 | T8210 | T8209 | pobj | ice,on |
R5933 | T8211 | T8206 | prep | with,pre-incubated |
R5934 | T8212 | T8213 | nummod | 4,µg |
R5935 | T8213 | T8211 | pobj | µg,with |
R5936 | T8214 | T8213 | prep | of,µg |
R5937 | T8215 | T8217 | amod | specific,antibody |
R5938 | T8216 | T8217 | compound | C/EBP,antibody |
R5939 | T8217 | T8214 | pobj | antibody,of |
R5940 | T8218 | T8205 | punct | .,were |
R5941 | T8219 | T8221 | nsubjpass | Antibodies,purchased |
R5942 | T8220 | T8221 | auxpass | were,purchased |
R5943 | T8221 | T8221 | ROOT | purchased,purchased |
R5944 | T8222 | T8221 | prep | from,purchased |
R5945 | T8223 | T8224 | compound | Santa,Cruz |
R5946 | T8224 | T8222 | pobj | Cruz,from |
R5947 | T8225 | T8228 | punct | :,α |
R5948 | T8226 | T8228 | amod | anti-C/EBP,α |
R5949 | T8227 | T8228 | punct | -,α |
R5950 | T8228 | T8249 | nmod | α,ε |
R5951 | T8229 | T8230 | punct | (,sc-61 |
R5952 | T8230 | T8228 | parataxis | sc-61,α |
R5953 | T8231 | T8230 | punct | ),sc-61 |
R5954 | T8232 | T8228 | punct | ",",α |
R5955 | T8233 | T8235 | amod | anti-C/EBP,β |
R5956 | T8234 | T8235 | punct | -,β |
R5957 | T8235 | T8228 | appos | β,α |
R5958 | T8236 | T8237 | punct | (,sc-150 |
R5959 | T8237 | T8235 | appos | sc-150,β |
R5960 | T8238 | T8237 | punct | ),sc-150 |
R5961 | T8239 | T8235 | punct | ",",β |
R5962 | T8240 | T8242 | amod | anti-C/EBP,δ |
R5963 | T8241 | T8242 | punct | -,δ |
R5964 | T8242 | T8228 | appos | δ,α |
R5965 | T8243 | T8244 | punct | (,sc-151 |
R5966 | T8244 | T8242 | appos | sc-151,δ |
R5967 | T8245 | T8244 | punct | ),sc-151 |
R5968 | T8246 | T8244 | cc | and,sc-151 |
R5969 | T8247 | T8249 | amod | anti-C/EBP,ε |
R5970 | T8248 | T8249 | punct | -,ε |
R5971 | T8249 | T8221 | npadvmod | ε,purchased |
R5972 | T8250 | T8251 | punct | (,sc-158 |
R5973 | T8251 | T8249 | appos | sc-158,ε |
R5974 | T8252 | T8251 | punct | ),sc-158 |
R5975 | T8253 | T8221 | punct | .,purchased |
pmc-enju-pas
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T8010 | 940-941 | -RRB- | denotes | ) |
T8009 | 934-940 | NN | denotes | sc-158 |
T8008 | 933-934 | -LRB- | denotes | ( |
T8007 | 920-932 | JJ | denotes | anti-C/EBP-ε |
T8006 | 916-919 | CC | denotes | and |
T8005 | 914-915 | -RRB- | denotes | ) |
T8004 | 908-914 | NN | denotes | sc-151 |
T8003 | 907-908 | -LRB- | denotes | ( |
T8002 | 894-906 | JJ | denotes | anti-C/EBP-δ |
T8001 | 892-893 | -COMMA- | denotes | , |
T8000 | 891-892 | -RRB- | denotes | ) |
T7999 | 885-891 | NN | denotes | sc-150 |
T7998 | 884-885 | -LRB- | denotes | ( |
T7997 | 871-883 | JJ | denotes | anti-C/EBP-β |
T7996 | 869-870 | -COMMA- | denotes | , |
T7995 | 868-869 | -RRB- | denotes | ) |
T7994 | 863-868 | NN | denotes | sc-61 |
T7993 | 862-863 | -LRB- | denotes | ( |
T7992 | 849-861 | JJ | denotes | anti-C/EBP-α |
T7991 | 847-848 | -COLON- | denotes | : |
T7990 | 843-847 | NNP | denotes | Cruz |
T7989 | 837-842 | NNP | denotes | Santa |
T7988 | 832-836 | IN | denotes | from |
T7987 | 822-831 | VB | denotes | purchased |
T7986 | 817-821 | VB | denotes | were |
T7985 | 806-816 | NN | denotes | Antibodies |
T7984 | 796-804 | NN | denotes | antibody |
T7983 | 790-795 | NN | denotes | C/EBP |
T7982 | 781-789 | JJ | denotes | specific |
T7981 | 778-780 | IN | denotes | of |
T7980 | 775-777 | NN | denotes | µg |
T7979 | 773-774 | CD | denotes | 4 |
T7978 | 768-772 | IN | denotes | with |
T7977 | 764-767 | NN | denotes | ice |
T7976 | 761-763 | IN | denotes | on |
T7975 | 757-760 | NN | denotes | hrs |
T7974 | 755-756 | CD | denotes | 2 |
T7973 | 741-754 | JJ | denotes | pre-incubated |
T7972 | 736-740 | VB | denotes | were |
T7971 | 727-735 | NN | denotes | extracts |
T7970 | 719-726 | JJ | denotes | nuclear |
T7969 | 717-718 | -COMMA- | denotes | , |
T7968 | 711-717 | NN | denotes | assays |
T7967 | 700-710 | NN | denotes | supershift |
T7966 | 696-699 | IN | denotes | For |
T7965 | 688-694 | NN | denotes | 10V/cm |
T7964 | 685-687 | IN | denotes | at |
T7963 | 681-684 | NN | denotes | TBE |
T7962 | 678-680 | NN | denotes | 1x |
T7961 | 675-677 | IN | denotes | in |
T7960 | 673-674 | -RRB- | denotes | ) |
T7959 | 672-673 | -RRB- | denotes | ) |
T7958 | 665-672 | NN | denotes | Bio-Rad |
T7957 | 664-665 | -LRB- | denotes | ( |
T7956 | 649-663 | NN | denotes | bis-acrylamide |
T7955 | 648-649 | -COLON- | denotes | : |
T7954 | 638-648 | NN | denotes | acrylamide |
T7953 | 633-637 | CD | denotes | 29∶1 |
T7952 | 632-633 | -LRB- | denotes | ( |
T7951 | 627-631 | NN | denotes | gels |
T7950 | 612-626 | NN | denotes | polyacrylamide |
T7949 | 610-611 | NN | denotes | % |
T7948 | 609-610 | CD | denotes | 5 |
T7947 | 606-608 | IN | denotes | on |
T7946 | 594-605 | NN | denotes | temperature |
T7945 | 589-593 | NN | denotes | room |
T7944 | 586-588 | IN | denotes | at |
T7943 | 570-585 | NN | denotes | electrophoresis |
T7942 | 567-569 | IN | denotes | by |
T7941 | 558-566 | VB | denotes | resolved |
T7940 | 553-557 | VB | denotes | were |
T7939 | 544-552 | NN | denotes | products |
T7938 | 535-543 | NN | denotes | reaction |
T7937 | 527-534 | NN | denotes | Binding |
T7936 | 514-525 | NN | denotes | temperature |
T7935 | 509-513 | NN | denotes | room |
T7934 | 506-508 | IN | denotes | at |
T7933 | 498-505 | NN | denotes | minutes |
T7932 | 495-497 | CD | denotes | 20 |
T7931 | 491-494 | IN | denotes | for |
T7930 | 481-490 | VB | denotes | performed |
T7929 | 476-480 | VB | denotes | were |
T7928 | 470-475 | NN | denotes | probe |
T7927 | 456-469 | VB | denotes | radiolabelled |
T7926 | 453-455 | IN | denotes | of |
T7925 | 451-452 | -RRB- | denotes | ) |
T7924 | 449-451 | NN | denotes | ng |
T7923 | 445-448 | CD | denotes | 0.2 |
T7922 | 442-444 | TO | denotes | to |
T7921 | 437-441 | CD | denotes | 0.05 |
T7920 | 436-437 | -LRB- | denotes | ( |
T7919 | 432-435 | NN | denotes | cpm |
T7918 | 425-431 | CD | denotes | 20,000 |
T7917 | 422-424 | TO | denotes | to |
T7916 | 415-421 | CD | denotes | 10,000 |
T7915 | 411-414 | CC | denotes | and |
T7914 | 402-410 | NN | denotes | extracts |
T7913 | 394-401 | JJ | denotes | nuclear |
T7912 | 391-393 | IN | denotes | of |
T7911 | 388-390 | NN | denotes | µg |
T7910 | 386-387 | CD | denotes | 6 |
T7909 | 384-385 | -COMMA- | denotes | , |
T7908 | 383-384 | -RRB- | denotes | ) |
T7907 | 378-383 | NN | denotes | dI-dC |
T7906 | 377-378 | -LRB- | denotes | ( |
T7905 | 372-376 | NN | denotes | poly |
T7904 | 369-371 | NN | denotes | µg |
T7903 | 367-368 | CD | denotes | 1 |
T7902 | 365-366 | -COMMA- | denotes | , |
T7901 | 355-365 | NN | denotes | Ficoll-400 |
T7900 | 353-354 | NN | denotes | % |
T7899 | 351-353 | CD | denotes | 20 |
T7898 | 349-350 | -COMMA- | denotes | , |
T7897 | 341-349 | NN | denotes | glycerol |
T7896 | 339-340 | NN | denotes | % |
T7895 | 337-339 | CD | denotes | 30 |
T7894 | 335-336 | -COMMA- | denotes | , |
T7893 | 332-335 | NN | denotes | DTT |
T7892 | 329-331 | NN | denotes | mM |
T7891 | 327-328 | CD | denotes | 2 |
T7890 | 325-326 | -COMMA- | denotes | , |
T7889 | 322-325 | NNP | denotes | BSA |
T7888 | 316-321 | NN | denotes | µg/ml |
T7887 | 313-315 | CD | denotes | 10 |
T7886 | 311-312 | -COMMA- | denotes | , |
T7885 | 308-311 | CD | denotes | 7.9 |
T7884 | 305-307 | NN | denotes | pH |
T7883 | 299-304 | NN | denotes | HEPES |
T7882 | 296-298 | NN | denotes | mM |
T7881 | 293-295 | CD | denotes | 10 |
T7880 | 282-292 | VB | denotes | containing |
T7879 | 280-281 | -RRB- | denotes | ) |
T7878 | 278-280 | NN | denotes | µl |
T7877 | 275-277 | CD | denotes | 25 |
T7876 | 274-275 | -LRB- | denotes | ( |
T7875 | 264-273 | NN | denotes | reactions |
T7874 | 256-263 | NN | denotes | binding |
T7873 | 252-255 | NN | denotes | DNA |
T7872 | 249-250 | -RRB- | denotes | ] |
T7871 | 247-249 | CD | denotes | 41 |
T7870 | 246-247 | -LRB- | denotes | [ |
T7869 | 239-245 | NN | denotes | method |
T7868 | 233-238 | NN | denotes | lysis |
T7867 | 223-232 | JJ | denotes | detergent |
T7866 | 219-222 | DT | denotes | the |
T7865 | 216-218 | IN | denotes | by |
T7864 | 207-215 | VB | denotes | prepared |
T7863 | 202-206 | VB | denotes | were |
T7862 | 193-201 | NN | denotes | extracts |
T7861 | 185-192 | JJ | denotes | nuclear |
T7860 | 179-184 | CD | denotes | 264.7 |
T7859 | 175-178 | NN | denotes | RAW |
T7858 | 169-173 | NN | denotes | -ATP |
T7857 | 168-169 | -RRB- | denotes | ] |
T7856 | 163-168 | NN | denotes | γ-32P |
T7855 | 162-163 | -LRB- | denotes | [ |
T7854 | 158-161 | CC | denotes | and |
T7853 | 151-157 | NN | denotes | kinase |
T7852 | 136-150 | NN | denotes | polynucleotide |
T7851 | 133-135 | NN | denotes | T4 |
T7850 | 127-132 | VB | denotes | using |
T7849 | 118-126 | VB | denotes | prepared |
T7848 | 113-117 | VB | denotes | were |
T7847 | 106-112 | NN | denotes | assays |
T7846 | 100-105 | NN | denotes | shift |
T7845 | 96-99 | NN | denotes | gel |
T7844 | 92-95 | IN | denotes | for |
T7843 | 85-91 | NN | denotes | probes |
T7842 | 69-84 | NN | denotes | oligonucleotide |
T7841 | 53-68 | JJ | denotes | double-stranded |
T7840 | 51-52 | -COMMA- | denotes | , |
T7839 | 38-51 | VB | denotes | Radiolabelled |
T7838 | 31-37 | NN | denotes | Assays |
T7837 | 25-30 | NN | denotes | Shift |
T7836 | 16-24 | NN | denotes | Mobility |
T7835 | 0-15 | JJ | denotes | Electrophoretic |
R5604 | T7838 | T7835 | arg1Of | Assays,Electrophoretic |
R5605 | T7838 | T7836 | arg1Of | Assays,Mobility |
R5606 | T7838 | T7837 | arg1Of | Assays,Shift |
R5607 | T7843 | T7839 | arg1Of | probes,Radiolabelled |
R5608 | T7843 | T7841 | arg1Of | probes,double-stranded |
R5609 | T7843 | T7842 | arg1Of | probes,oligonucleotide |
R5610 | T7843 | T7844 | arg1Of | probes,for |
R5611 | T7843 | T7848 | arg1Of | probes,were |
R5612 | T7843 | T7849 | arg2Of | probes,prepared |
R5613 | T7847 | T7844 | arg2Of | assays,for |
R5614 | T7847 | T7845 | arg1Of | assays,gel |
R5615 | T7847 | T7846 | arg1Of | assays,shift |
R5616 | T7849 | T7839 | modOf | prepared,Radiolabelled |
R5617 | T7849 | T7840 | arg1Of | prepared,"," |
R5618 | T7849 | T7848 | arg2Of | prepared,were |
R5619 | T7849 | T7850 | modOf | prepared,using |
R5620 | T7853 | T7851 | arg1Of | kinase,T4 |
R5621 | T7853 | T7852 | arg1Of | kinase,polynucleotide |
R5622 | T7853 | T7854 | arg1Of | kinase,and |
R5623 | T7854 | T7850 | arg2Of | and,using |
R5624 | T7856 | T7855 | arg2Of | γ-32P,[ |
R5625 | T7857 | T7855 | arg3Of | ],[ |
R5626 | T7858 | T7854 | arg2Of | -ATP,and |
R5627 | T7858 | T7855 | arg1Of | -ATP,[ |
R5628 | T7862 | T7859 | arg1Of | extracts,RAW |
R5629 | T7862 | T7860 | arg1Of | extracts,264.7 |
R5630 | T7862 | T7861 | arg1Of | extracts,nuclear |
R5631 | T7862 | T7863 | arg1Of | extracts,were |
R5632 | T7862 | T7864 | arg2Of | extracts,prepared |
R5633 | T7864 | T7863 | arg2Of | prepared,were |
R5634 | T7864 | T7870 | arg1Of | prepared,[ |
R5635 | T7869 | T7864 | arg1Of | method,prepared |
R5636 | T7869 | T7865 | arg2Of | method,by |
R5637 | T7869 | T7866 | arg1Of | method,the |
R5638 | T7869 | T7867 | arg1Of | method,detergent |
R5639 | T7869 | T7868 | arg1Of | method,lysis |
R5640 | T7871 | T7870 | arg2Of | 41,[ |
R5641 | T7872 | T7870 | arg3Of | ],[ |
R5642 | T7875 | T7873 | arg1Of | reactions,DNA |
R5643 | T7875 | T7874 | arg1Of | reactions,binding |
R5644 | T7875 | T7876 | arg1Of | reactions,( |
R5645 | T7875 | T7880 | arg1Of | reactions,containing |
R5646 | T7875 | T7890 | arg1Of | reactions,"," |
R5647 | T7875 | T7909 | arg1Of | reactions,"," |
R5648 | T7878 | T7876 | arg2Of | µl,( |
R5649 | T7878 | T7877 | arg1Of | µl,25 |
R5650 | T7879 | T7876 | arg3Of | ),( |
R5651 | T7881 | T7882 | arg1Of | 10,mM |
R5652 | T7885 | T7886 | arg1Of | 7.9,"," |
R5653 | T7888 | T7886 | arg2Of | µg/ml,"," |
R5654 | T7888 | T7887 | arg1Of | µg/ml,10 |
R5655 | T7889 | T7880 | arg2Of | BSA,containing |
R5656 | T7889 | T7881 | arg1Of | BSA,10 |
R5657 | T7889 | T7883 | arg1Of | BSA,HEPES |
R5658 | T7889 | T7884 | arg1Of | BSA,pH |
R5659 | T7889 | T7885 | arg1Of | BSA,7.9 |
R5660 | T7889 | T7888 | arg1Of | BSA,µg/ml |
R5661 | T7891 | T7892 | arg1Of | 2,mM |
R5662 | T7893 | T7891 | arg1Of | DTT,2 |
R5663 | T7893 | T7894 | arg1Of | DTT,"," |
R5664 | T7894 | T7898 | arg1Of | ",","," |
R5665 | T7895 | T7896 | arg1Of | 30,% |
R5666 | T7897 | T7894 | arg2Of | glycerol,"," |
R5667 | T7897 | T7895 | arg1Of | glycerol,30 |
R5668 | T7898 | T7902 | arg1Of | ",","," |
R5669 | T7899 | T7900 | arg1Of | 20,% |
R5670 | T7901 | T7898 | arg2Of | Ficoll-400,"," |
R5671 | T7901 | T7899 | arg1Of | Ficoll-400,20 |
R5672 | T7902 | T7890 | arg2Of | ",","," |
R5673 | T7905 | T7902 | arg2Of | poly,"," |
R5674 | T7905 | T7903 | arg1Of | poly,1 |
R5675 | T7905 | T7904 | arg1Of | poly,µg |
R5676 | T7905 | T7906 | arg1Of | poly,( |
R5677 | T7907 | T7906 | arg2Of | dI-dC,( |
R5678 | T7908 | T7906 | arg3Of | ),( |
R5679 | T7909 | T7929 | arg1Of | ",",were |
R5680 | T7909 | T7930 | arg2Of | ",",performed |
R5681 | T7911 | T7910 | arg1Of | µg,6 |
R5682 | T7911 | T7912 | arg1Of | µg,of |
R5683 | T7911 | T7915 | arg1Of | µg,and |
R5684 | T7914 | T7912 | arg2Of | extracts,of |
R5685 | T7914 | T7913 | arg1Of | extracts,nuclear |
R5686 | T7915 | T7909 | arg2Of | and,"," |
R5687 | T7915 | T7926 | arg1Of | and,of |
R5688 | T7916 | T7917 | arg1Of | "10,000",to |
R5689 | T7918 | T7917 | arg2Of | "20,000",to |
R5690 | T7919 | T7915 | arg2Of | cpm,and |
R5691 | T7919 | T7916 | arg1Of | cpm,"10,000" |
R5692 | T7919 | T7920 | arg1Of | cpm,( |
R5693 | T7921 | T7922 | arg1Of | 0.05,to |
R5694 | T7923 | T7922 | arg2Of | 0.2,to |
R5695 | T7924 | T7920 | arg2Of | ng,( |
R5696 | T7924 | T7921 | arg1Of | ng,0.05 |
R5697 | T7925 | T7920 | arg3Of | ),( |
R5698 | T7928 | T7926 | arg2Of | probe,of |
R5699 | T7928 | T7927 | arg2Of | probe,radiolabelled |
R5700 | T7930 | T7929 | arg2Of | performed,were |
R5701 | T7930 | T7931 | arg1Of | performed,for |
R5702 | T7933 | T7931 | arg2Of | minutes,for |
R5703 | T7933 | T7932 | arg1Of | minutes,20 |
R5704 | T7933 | T7934 | arg1Of | minutes,at |
R5705 | T7936 | T7934 | arg2Of | temperature,at |
R5706 | T7936 | T7935 | arg1Of | temperature,room |
R5707 | T7939 | T7937 | arg1Of | products,Binding |
R5708 | T7939 | T7938 | arg1Of | products,reaction |
R5709 | T7939 | T7940 | arg1Of | products,were |
R5710 | T7939 | T7941 | arg2Of | products,resolved |
R5711 | T7941 | T7940 | arg2Of | resolved,were |
R5712 | T7941 | T7942 | arg1Of | resolved,by |
R5713 | T7943 | T7942 | arg2Of | electrophoresis,by |
R5714 | T7943 | T7944 | arg1Of | electrophoresis,at |
R5715 | T7946 | T7944 | arg2Of | temperature,at |
R5716 | T7946 | T7945 | arg1Of | temperature,room |
R5717 | T7946 | T7947 | arg1Of | temperature,on |
R5718 | T7946 | T7961 | arg1Of | temperature,in |
R5719 | T7948 | T7949 | arg1Of | 5,% |
R5720 | T7951 | T7947 | arg2Of | gels,on |
R5721 | T7951 | T7948 | arg1Of | gels,5 |
R5722 | T7951 | T7950 | arg1Of | gels,polyacrylamide |
R5723 | T7951 | T7952 | arg1Of | gels,( |
R5724 | T7954 | T7953 | arg1Of | acrylamide,29∶1 |
R5725 | T7954 | T7955 | arg1Of | acrylamide,: |
R5726 | T7955 | T7952 | arg2Of | :,( |
R5727 | T7956 | T7955 | arg2Of | bis-acrylamide,: |
R5728 | T7956 | T7957 | arg1Of | bis-acrylamide,( |
R5729 | T7958 | T7957 | arg2Of | Bio-Rad,( |
R5730 | T7959 | T7957 | arg3Of | ),( |
R5731 | T7960 | T7952 | arg3Of | ),( |
R5732 | T7963 | T7961 | arg2Of | TBE,in |
R5733 | T7963 | T7962 | arg1Of | TBE,1x |
R5734 | T7963 | T7964 | arg1Of | TBE,at |
R5735 | T7965 | T7964 | arg2Of | 10V/cm,at |
R5736 | T7968 | T7966 | arg2Of | assays,For |
R5737 | T7968 | T7967 | arg1Of | assays,supershift |
R5738 | T7971 | T7970 | arg1Of | extracts,nuclear |
R5739 | T7971 | T7972 | arg1Of | extracts,were |
R5740 | T7972 | T7966 | arg1Of | were,For |
R5741 | T7972 | T7969 | arg1Of | were,"," |
R5742 | T7974 | T7973 | arg1Of | 2,pre-incubated |
R5743 | T7975 | T7972 | arg2Of | hrs,were |
R5744 | T7975 | T7974 | arg1Of | hrs,2 |
R5745 | T7975 | T7976 | arg1Of | hrs,on |
R5746 | T7977 | T7976 | arg2Of | ice,on |
R5747 | T7977 | T7978 | arg1Of | ice,with |
R5748 | T7980 | T7978 | arg2Of | µg,with |
R5749 | T7980 | T7979 | arg1Of | µg,4 |
R5750 | T7980 | T7981 | arg1Of | µg,of |
R5751 | T7984 | T7981 | arg2Of | antibody,of |
R5752 | T7984 | T7982 | arg1Of | antibody,specific |
R5753 | T7984 | T7983 | arg1Of | antibody,C/EBP |
R5754 | T7985 | T7986 | arg1Of | Antibodies,were |
R5755 | T7985 | T7987 | arg2Of | Antibodies,purchased |
R5756 | T7987 | T7986 | arg2Of | purchased,were |
R5757 | T7987 | T7988 | arg1Of | purchased,from |
R5758 | T7990 | T7988 | arg2Of | Cruz,from |
R5759 | T7990 | T7989 | arg1Of | Cruz,Santa |
R5760 | T7990 | T7991 | arg1Of | Cruz,: |
R5761 | T7992 | T7993 | arg1Of | anti-C/EBP-α,( |
R5762 | T7992 | T7996 | arg1Of | anti-C/EBP-α,"," |
R5763 | T7994 | T7993 | arg2Of | sc-61,( |
R5764 | T7995 | T7993 | arg3Of | ),( |
R5765 | T7996 | T8001 | arg1Of | ",","," |
R5766 | T7997 | T7996 | arg2Of | anti-C/EBP-β,"," |
R5767 | T7997 | T7998 | arg1Of | anti-C/EBP-β,( |
R5768 | T7999 | T7998 | arg2Of | sc-150,( |
R5769 | T8000 | T7998 | arg3Of | ),( |
R5770 | T8001 | T7991 | arg2Of | ",",: |
R5771 | T8002 | T8003 | arg1Of | anti-C/EBP-δ,( |
R5772 | T8002 | T8006 | arg1Of | anti-C/EBP-δ,and |
R5773 | T8004 | T8003 | arg2Of | sc-151,( |
R5774 | T8005 | T8003 | arg3Of | ),( |
R5775 | T8006 | T8001 | arg2Of | and,"," |
R5776 | T8007 | T8006 | arg2Of | anti-C/EBP-ε,and |
R5777 | T8007 | T8008 | arg1Of | anti-C/EBP-ε,( |
R5778 | T8009 | T8008 | arg2Of | sc-158,( |
R5779 | T8010 | T8008 | arg3Of | ),( |
bionlp-st-ge-2016-test-proteins
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T7825 | 133-157 | Protein | denotes | T4 polynucleotide kinase |
bionlp-st-ge-2016-uniprot
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T8012 | 876-883 | http://www.uniprot.org/uniprot/P28033 | denotes | C/EBP-β |
GO-BP
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T7826 | 233-238 | http://purl.obolibrary.org/obo/GO_0019835 | denotes | lysis |
GO-MF
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T7830 | 796-804 | http://purl.obolibrary.org/obo/GO_0003823 | denotes | antibody |
T7829 | 527-534 | http://purl.obolibrary.org/obo/GO_0005488 | denotes | Binding |
T7828 | 256-263 | http://purl.obolibrary.org/obo/GO_0005488 | denotes | binding |
T7827 | 252-263 | http://purl.obolibrary.org/obo/GO_0003677 | denotes | DNA binding |
GO-CC
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T7832 | 796-804 | http://purl.obolibrary.org/obo/GO_0042571 | denotes | antibody |
T7831 | 796-804 | http://purl.obolibrary.org/obo/GO_0019815 | denotes | antibody |
sentences
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T7823 | 806-942 | Sentence | denotes | Antibodies were purchased from Santa Cruz: anti-C/EBP-α (sc-61), anti-C/EBP-β (sc-150), anti-C/EBP-δ (sc-151) and anti-C/EBP-ε (sc-158). |
T7822 | 696-805 | Sentence | denotes | For supershift assays, nuclear extracts were pre-incubated 2 hrs on ice with 4 µg of specific C/EBP antibody. |
T7821 | 527-695 | Sentence | denotes | Binding reaction products were resolved by electrophoresis at room temperature on 5% polyacrylamide gels (29∶1 acrylamide:bis-acrylamide (Bio-Rad)) in 1x TBE at 10V/cm. |
T7820 | 252-526 | Sentence | denotes | DNA binding reactions (25 µl) containing 10 mM HEPES pH 7.9, 10 µg/ml BSA, 2 mM DTT, 30% glycerol, 20% Ficoll-400, 1 µg poly (dI-dC), 6 µg of nuclear extracts and 10,000 to 20,000 cpm (0.05 to 0.2 ng) of radiolabelled probe were performed for 20 minutes at room temperature. |
T7819 | 175-251 | Sentence | denotes | RAW 264.7 nuclear extracts were prepared by the detergent lysis method [41]. |
T7818 | 38-174 | Sentence | denotes | Radiolabelled, double-stranded oligonucleotide probes for gel shift assays were prepared using T4 polynucleotide kinase and [γ-32P]-ATP. |
T7817 | 0-37 | Sentence | denotes | Electrophoretic Mobility Shift Assays |
T91 | 0-37 | Sentence | denotes | Electrophoretic Mobility Shift Assays |
T92 | 38-174 | Sentence | denotes | Radiolabelled, double-stranded oligonucleotide probes for gel shift assays were prepared using T4 polynucleotide kinase and [γ-32P]-ATP. |
T93 | 175-251 | Sentence | denotes | RAW 264.7 nuclear extracts were prepared by the detergent lysis method [41]. |
T94 | 252-526 | Sentence | denotes | DNA binding reactions (25 µl) containing 10 mM HEPES pH 7.9, 10 µg/ml BSA, 2 mM DTT, 30% glycerol, 20% Ficoll-400, 1 µg poly (dI-dC), 6 µg of nuclear extracts and 10,000 to 20,000 cpm (0.05 to 0.2 ng) of radiolabelled probe were performed for 20 minutes at room temperature. |
T95 | 527-695 | Sentence | denotes | Binding reaction products were resolved by electrophoresis at room temperature on 5% polyacrylamide gels (29∶1 acrylamide:bis-acrylamide (Bio-Rad)) in 1x TBE at 10V/cm. |
T96 | 696-805 | Sentence | denotes | For supershift assays, nuclear extracts were pre-incubated 2 hrs on ice with 4 µg of specific C/EBP antibody. |
T97 | 806-942 | Sentence | denotes | Antibodies were purchased from Santa Cruz: anti-C/EBP-α (sc-61), anti-C/EBP-β (sc-150), anti-C/EBP-δ (sc-151) and anti-C/EBP-ε (sc-158). |
simple1
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T7834 | 133-157 | Protein | denotes | T4 polynucleotide kinase |
BioNLP16_DUT
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T8011 | 133-157 | Protein | denotes | T4 polynucleotide kinase |
BioNLP16_Messiy
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T8015 | 133-157 | Protein | denotes | T4 polynucleotide kinase |
DLUT931
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T8013 | 133-157 | Protein | denotes | T4 polynucleotide kinase |
bionlp-st-ge-2016-test-ihmc
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T8058 | 806-847 | Binding | denotes | Antibodies were purchased from Santa Cruz |
T8057 | 764-804 | Entity | denotes | ice with 4 µg of specific C/EBP antibody |
T8056 | 175-201 | Entity | denotes | RAW 264.7 nuclear extracts |
T8055 | 432-435 | Protein | denotes | cpm |
T8054 | 337-349 | Entity | denotes | 30% glycerol |
T8053 | 394-410 | Entity | denotes | nuclear extracts |
T8052 | 653-663 | Entity | denotes | acrylamide |
T8051 | 293-311 | Entity | denotes | 10 mM HEPES pH 7.9 |
T8050 | 741-804 | Protein | denotes | pre-incubated 2 hrs on ice with 4 µg of specific C/EBP antibody |
T8049 | 837-842 | Protein | denotes | Santa |
T8048 | 118-157 | Protein | denotes | prepared using T4 polynucleotide kinase |
T8047 | 894-915 | Protein | denotes | anti-C/EBP-δ (sc-151) |
T8046 | 719-735 | Entity | denotes | nuclear extracts |
T8045 | 790-795 | Protein | denotes | C/EBP |
T8044 | 53-84 | Entity | denotes | double-stranded oligonucleotide |
T8043 | 700-717 | Entity | denotes | supershift assays |
T8042 | 636-648 | Entity | denotes | 1 acrylamide |
T8041 | 806-816 | Entity | denotes | Antibodies |
T8040 | 367-384 | Entity | denotes | 1 µg poly (dI-dC) |
T8039 | 649-663 | Protein | denotes | bis-acrylamide |
T8038 | 612-626 | Entity | denotes | polyacrylamide |
T8037 | 133-135 | Entity | denotes | T4 |
T8036 | 778-804 | Entity | denotes | of specific C/EBP antibody |
T8035 | 322-350 | Entity | denotes | BSA, 2 mM DTT, 30% glycerol, |
T8034 | 649-663 | Entity | denotes | bis-acrylamide |
T8033 | 609-631 | Entity | denotes | 5% polyacrylamide gels |
T8032 | 773-804 | Entity | denotes | 4 µg of specific C/EBP antibody |
T8031 | 169-173 | Entity | denotes | -ATP |
T8030 | 216-250 | Entity | denotes | by the detergent lysis method [41] |
R5780 | T8041 | T8058 | themeOf | Antibodies,Antibodies were purchased from Santa Cruz |
bionlp-st-ge-2016-test-tees
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T8022 | 920-932 | Protein | denotes | anti-C/EBP-ε |
T8021 | 894-906 | Protein | denotes | anti-C/EBP-δ |
T8020 | 871-883 | Protein | denotes | anti-C/EBP-β |
T8019 | 849-861 | Protein | denotes | anti-C/EBP-α |
T8018 | 790-804 | Protein | denotes | C/EBP antibody |
T8017 | 669-672 | Protein | denotes | Rad |
T8016 | 133-157 | Protein | denotes | T4 polynucleotide kinase |
testone
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T7814 | 133-157 | Protein | denotes | T4 polynucleotide kinase |
test3
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T7816 | 133-157 | Protein | denotes | T4 polynucleotide kinase |
T7815 | 133-157 | Protein | denotes | T4 polynucleotide kinase |