qRT-PCR Cells were treated as described for Western blots. Total RNA was isolated using ZR Miniprep kit (Zymo Research). 1 µg of RNA was converted to cDNA using random primers (M-MuLV cDNA kit, New England Biolabs). 1 µL of cDNA was used with 500 pM primers in qPCR reactions. Reactions were performs using SYBRGreen 2×Master mix (SABiosciences) in a LightCycler480 (Roche).