Figure 2 ODN 2216 induces an antiviral gene expression profile in PBMCs of adult cats. (A-D) mRNA expression factors of the indicated genes were measured in PBMCs isolated from fourteen cats belonging to the indicated age groups and stimulated with ODN 2216 for 24 h. (E) mRNA expression factors of the indicated IFNα subtype genes were measured in PBMCs isolated from four adult cats (group 2) and stimulated with ODN 2216 for 24 h. (F) mRNA levels of TLR9 were measured by real-time qPCR in unstimulated PBMCs of all four groups of cats and normalized to the expression of a feline housekeeping gene (GAPDH). (G) Relative TLR9 mRNA expression factors were measured in ODN 2216 stimulated PBMCs from cats of all four groups. *p < 0.05, Perf = perforin, GranB = granzyme B.