Table 1  Media used in studies of influenza A virus viability versus RH.
Study  Media  Salt content  Protein content  Other components  Reference
Hemmes et al. 1960  1 part allantoic fluid and one part2% Difco peptone  ∼2.3 g L−1 (0.4 g L−1 K+, 0.9 g L−1 Na+,0.9 g L−1 Cl−)a  10 g L−1 peptone  -b  [8], [43]
Harper 1961  Allantoic fluid diluted 1∶8 or 1∶10 incasein McIlvaine’s buffer (pH 7.2)  ∼2.2 g L−1 (mainly Na2HPO4) a  ∼1.9 g L−1 mainly casein  -b  [13], [43], [44]
Shechmeister 1950  Allantoic fluid in 0.1 M Sorensen’sphosphate buffer (pH 7.1)  19.6 g L−1 (8.09 g L−1 NaH2PO4, 9.51 gL−1 Na2HPO4)  ∼1 g L−1 in allantoicfluid  -b  [15], [43]
Schaffer et al. 1976  MEM  9.88 g L−1 (6.8 g L−1 NaCl, 2.2 g L−1NaHCO3, and others)  0  Amino acids, vitamins,glucose, others  [14], [43], [45]
MEM+0.1% BSA  9.88 g L−1  1 g L−1  Same as above
Allantoic fluid  ∼4.7 g L−1 (0.8 g L−1 K+, 1.8 g L−1 Na+,1.8 g L−1 Cl−)a  ∼1 g L−1
This work  PBS (pH 7.2)  9.55 g L−1 (8 g L−1 NaCl, 0.2 g L−1 KCl,1.15 g L−1 Na2HPO4, 0.2 g L−1 KH2PO4)  0  None
PBS+5% fetal calf serum (FCS)  Same as above  ∼3.5 g L−1  Other componentsfrom FCS
Dulbecco's modified Eaglemedium (DMEM)  10.92 g L−1 (6.4 g L−1 NaCl, 3.7 g L−1NaHCO3)  0.42 g L−1  Amino acids, vitamins,glucose, others
DMEM+5%FCS  Same as above  ∼3.9 g L−1  Same as above
a  Estimated;
b  Detailed composition of allantoic fluid is unknown.