In this study, droplets of 1 µL, rather than smaller aerosols [42], were used to simulate the interplay of humidity, droplet evaporation, solute concentrations in the droplet, and virus viability. It takes ∼10 min for such droplets to dry out completely at ∼50% RH, considerably longer than for much smaller droplets (e.g., <1 s for a respiratory droplet 20 µm in diameter [17]). The legitimacy of extrapolating our results to aerosols expelled from human respiratory tract thus depends on whether the dynamics of evaporation are critical for IAV decay. Our results in model media are comparable to those conducted in aerosols [8], [13]. Harper [13] recovered 66–126% of IAV in aerosols 1 s after spraying, when evaporation was completed. The high recoveries indicate that the effect of the evaporation process itself on viral decay is negligible. Even if it were critical, such an effect was controlled for when comparing viral decay in the same type of medium versus RH, since all the droplets experienced a similar rate of evaporation. Nevertheless, verifying these results in aerosols is warranted.