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PMC:3342329 / 29830-31727 JSONTXT

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pmc-enju-pas

Id Subject Object Predicate Lexical cue
T14927 1387-1396 NN denotes detection
T14926 1383-1386 DT denotes the
T14925 1379-1382 IN denotes For
T14924 1369-1377 NN denotes antibody
T14923 1361-1368 JJ denotes primary
T14922 1357-1360 DT denotes the
T14921 1348-1356 VB denotes omitting
T14920 1345-1347 IN denotes by
T14919 1333-1344 NN denotes experiments
T14918 1329-1332 DT denotes all
T14917 1326-1328 IN denotes in
T14916 1317-1325 VB denotes included
T14915 1313-1316 VB denotes was
T14914 1305-1312 NN denotes control
T14913 1296-1304 JJ denotes negative
T14912 1294-1295 DT denotes A
T14911 1291-1292 -RRB- denotes )
T14910 1286-1291 NNP denotes Japan
T14909 1284-1285 -COMMA- denotes ,
T14908 1279-1284 NNP denotes Tokyo
T14907 1277-1278 -COMMA- denotes ,
T14906 1270-1277 NNP denotes Olympus
T14905 1268-1269 -COLON- denotes ;
T14904 1251-1268 NN denotes magnification×200
T14903 1250-1251 -LRB- denotes (
T14902 1239-1249 NN denotes microscope
T14901 1233-1238 JJ denotes light
T14900 1231-1232 DT denotes a
T14899 1225-1230 IN denotes under
T14898 1214-1224 NN denotes evaluation
T14897 1210-1213 CC denotes and
T14896 1208-1209 -COMMA- denotes ,
T14895 1198-1208 JJ denotes aqua-mount
T14894 1195-1197 IN denotes in
T14893 1186-1194 VB denotes mounting
T14892 1184-1185 -COMMA- denotes ,
T14891 1173-1184 NN denotes hematoxylin
T14890 1168-1172 IN denotes with
T14889 1152-1167 VB denotes counterstaining
T14888 1149-1151 IN denotes by
T14887 1140-1148 VB denotes followed
T14886 1138-1139 -COMMA- denotes ,
T14885 1130-1138 NN denotes peroxide
T14884 1126-1129 CC denotes and
T14883 1124-1125 -RRB- denotes )
T14882 1121-1124 NN denotes DAB
T14881 1120-1121 -LRB- denotes (
T14880 1103-1119 NN denotes diaminobenzidine
T14879 1098-1102 IN denotes with
T14878 1088-1097 VB denotes developed
T14877 1084-1087 VB denotes was
T14876 1075-1083 NN denotes reaction
T14875 1064-1074 NN denotes peroxidase
T14874 1060-1063 DT denotes the
T14873 1056-1059 CC denotes and
T14872 1049-1055 VB denotes washed
T14871 1044-1048 VB denotes were
T14870 1037-1043 NN denotes slides
T14869 1033-1036 DT denotes The
T14868 1030-1031 -RRB- denotes )
T14867 1027-1030 NNP denotes USA
T14866 1025-1026 -COMMA- denotes ,
T14865 1023-1025 NNP denotes CA
T14864 1021-1022 -COMMA- denotes ,
T14863 1011-1021 NNP denotes Burlingame
T14862 1009-1010 -COMMA- denotes ,
T14861 997-1009 NNP denotes Laboratories
T14860 990-996 NNP denotes Vector
T14859 988-989 -COLON- denotes ;
T14858 985-988 NN denotes kit
T14857 981-984 NN denotes ABC
T14856 980-981 -LRB- denotes (
T14855 972-979 NN denotes complex
T14854 961-971 NN denotes peroxidase
T14853 954-960 NN denotes biotin
T14852 947-953 NN denotes avidin
T14851 943-946 DT denotes the
T14850 940-942 IN denotes of
T14849 931-939 NN denotes addition
T14848 927-930 DT denotes the
T14847 924-926 IN denotes by
T14846 915-923 VB denotes followed
T14845 913-914 -COMMA- denotes ,
T14844 910-913 NNP denotes PBS
T14843 907-909 IN denotes in
T14842 900-906 VB denotes washed
T14841 895-899 VB denotes were
T14840 888-894 NN denotes slides
T14839 884-887 DT denotes The
T14838 881-882 NN denotes h
T14837 879-880 CD denotes 2
T14836 875-878 IN denotes for
T14835 864-874 NN denotes antibodies
T14834 852-863 JJ denotes anti-rabbit
T14833 848-851 CC denotes and
T14832 837-847 JJ denotes anti-mouse
T14831 824-836 VB denotes biotinylated
T14830 819-823 IN denotes with
T14829 809-818 VB denotes incubated
T14828 805-808 CC denotes and
T14827 801-804 NNP denotes PBS
T14826 798-800 IN denotes in
T14825 793-797 DT denotes each
T14824 789-792 NN denotes min
T14823 787-788 CD denotes 5
T14822 783-786 IN denotes for
T14821 777-782 NN denotes times
T14820 771-776 CD denotes three
T14819 764-770 VB denotes washed
T14818 759-763 VB denotes were
T14817 752-758 NN denotes slides
T14816 748-751 DT denotes the
T14815 746-747 -COMMA- denotes ,
T14814 736-746 RB denotes Thereafter
T14813 723-734 NN denotes temperature
T14812 718-722 NN denotes room
T14811 715-717 IN denotes at
T14810 713-714 NN denotes h
T14809 711-712 CD denotes 4
T14808 707-710 IN denotes for
T14807 701-706 NN denotes serum
T14806 692-700 VB denotes blocking
T14805 689-691 IN denotes in
T14804 678-688 NN denotes antibodies
T14803 667-677 JJ denotes monoclonal
T14802 665-666 -RRB- denotes )
T14801 657-665 NN denotes dilution
T14800 651-656 CD denotes 1∶200
T14799 649-650 -COMMA- denotes ,
T14798 645-649 NN denotes PCNA
T14797 644-645 -LRB- denotes (
T14796 636-643 NN denotes antigen
T14795 628-635 JJ denotes nuclear
T14794 623-627 NN denotes cell
T14793 609-622 VB denotes proliferating
T14792 598-608 JJ denotes anti-mouse
T14791 592-597 NN denotes mouse
T14790 584-591 JJ denotes primary
T14789 579-583 IN denotes with
T14788 569-578 VB denotes incubated
T14787 565-568 CC denotes and
T14786 563-564 -COMMA- denotes ,
T14785 556-563 VB denotes blotted
T14784 554-555 -COMMA- denotes ,
T14783 551-554 NNP denotes PBS
T14782 548-550 IN denotes in
T14781 540-547 VB denotes diluted
T14780 534-539 NN denotes serum
T14779 528-533 NN denotes horse
T14778 521-527 JJ denotes normal
T14777 519-520 NN denotes %
T14776 518-519 CD denotes 3
T14775 513-517 IN denotes with
T14774 509-512 NN denotes min
T14773 506-508 CD denotes 30
T14772 502-505 IN denotes for
T14771 494-501 VB denotes blocked
T14770 489-493 VB denotes were
T14769 480-488 NN denotes sections
T14768 476-479 DT denotes The
T14767 471-474 NN denotes min
T14766 469-470 CD denotes 5
T14765 465-468 IN denotes for
T14764 461-464 NNP denotes PBS
T14763 458-460 IN denotes in
T14762 449-457 VB denotes clearing
T14761 446-448 IN denotes by
T14760 437-445 VB denotes followed
T14759 435-436 -COMMA- denotes ,
T14758 432-435 NN denotes min
T14757 429-431 CD denotes 15
T14756 425-428 IN denotes for
T14755 416-424 NN denotes methanol
T14754 413-415 IN denotes in
T14753 404-412 NN denotes peroxide
T14752 395-403 NN denotes hydrogen
T14751 393-394 NN denotes %
T14750 392-393 CD denotes 3
T14749 389-391 IN denotes in
T14748 378-388 NN denotes incubation
T14747 375-377 IN denotes by
T14746 366-374 VB denotes quenched
T14745 362-365 VB denotes was
T14744 353-361 NN denotes activity
T14743 342-352 NN denotes peroxidase
T14742 331-341 JJ denotes Endogenous
T14741 320-329 NN denotes retrieval
T14740 312-319 NN denotes antigen
T14739 298-311 JJ denotes heat-mediated
T14738 295-297 TO denotes to
T14737 285-294 VB denotes subjected
T14736 280-284 RB denotes then
T14735 276-279 CC denotes and
T14734 274-275 -COMMA- denotes ,
T14733 269-274 NN denotes water
T14732 259-268 JJ denotes distilled
T14731 256-258 IN denotes in
T14730 249-255 VB denotes washed
T14729 247-248 -COMMA- denotes ,
T14728 237-247 VB denotes rehydrated
T14727 233-236 CC denotes and
T14726 218-232 VB denotes deparaffinized
T14725 213-217 VB denotes were
T14724 204-212 NN denotes sections
T14723 186-203 JJ denotes paraffin-embedded
T14722 182-185 DT denotes The
T14721 160-180 NN denotes immunohistochemistry
T14720 157-159 IN denotes by
T14719 148-156 VB denotes analyzed
T14718 144-147 CC denotes and
T14717 142-143 NN denotes E
T14716 141-142 CC denotes &
T14715 140-141 NN denotes H
T14714 135-139 IN denotes with
T14713 127-134 VB denotes stained
T14712 122-126 VB denotes were
T15022 1895-1896 -RRB- denotes )
T15021 1885-1895 NN denotes microscope
T15020 1881-1884 NN denotes DAS
T15019 1880-1881 -LRB- denotes (
T15018 1869-1879 NN denotes microscopy
T15017 1856-1868 NN denotes fluorescence
T15016 1852-1855 IN denotes for
T15015 1838-1851 JJ denotes cover-slipped
T15014 1834-1837 CC denotes and
T15013 1832-1833 -COMMA- denotes ,
T15012 1826-1832 NN denotes slides
T15011 1823-1825 IN denotes on
T15010 1815-1822 VB denotes mounted
T15009 1813-1814 -COMMA- denotes ,
T15008 1807-1813 VB denotes rinsed
T15007 1802-1806 VB denotes were
T15006 1793-1801 NN denotes sections
T15005 1789-1792 DT denotes the
T15004 1787-1788 -COMMA- denotes ,
T15003 1780-1787 NNP denotes Finally
T15002 1774-1779 NNP denotes 37°C.
T15001 1771-1773 IN denotes at
T15000 1767-1770 NN denotes min
T14999 1764-1766 CD denotes 15
T14998 1760-1763 IN denotes for
T14997 1758-1759 -RRB- denotes )
T14996 1754-1758 NN denotes DAPI
T14995 1753-1754 -LRB- denotes (
T14994 1726-1752 NN denotes 6-diamidino-2-phenylindole
T14993 1725-1726 -COMMA- denotes ,
T14992 1723-1725 NN denotes 4′
T14991 1718-1722 IN denotes with
T14990 1708-1717 VB denotes incubated
T14989 1703-1707 VB denotes were
T14988 1694-1702 NN denotes sections
T14987 1690-1693 DT denotes the
T14986 1688-1689 -COMMA- denotes ,
T14985 1684-1688 RB denotes Next
T14984 1670-1682 NN denotes instructions
T14983 1655-1669 NN denotes manufacturer’s
T14982 1651-1654 DT denotes the
T14981 1648-1650 TO denotes to
T14980 1638-1647 VB denotes according
T14979 1636-1637 -COMMA- denotes ,
T14978 1632-1636 DT denotes each
T14977 1628-1631 NN denotes min
T14976 1626-1627 CD denotes 5
T14975 1622-1625 IN denotes for
T14974 1618-1621 NNP denotes PBS
T14973 1616-1617 NN denotes M
T14972 1612-1615 CD denotes 0.1
T14971 1609-1611 IN denotes in
T14970 1603-1608 NN denotes times
T14969 1597-1602 CD denotes three
T14968 1590-1596 VB denotes washed
T14967 1585-1589 RB denotes then
T14966 1581-1584 CC denotes and
T14965 1579-1580 -COMMA- denotes ,
T14964 1575-1579 NN denotes 37°C
T14963 1572-1574 IN denotes at
T14962 1570-1571 NN denotes h
T14961 1568-1569 CD denotes 1
T14960 1564-1567 IN denotes for
T14959 1562-1563 -RRB- denotes )
T14958 1560-1562 NN denotes µl
T14957 1557-1559 CD denotes 60
T14956 1556-1557 -LRB- denotes (
T14955 1547-1555 NN denotes solution
T14954 1540-1546 NN denotes enzyme
T14953 1536-1539 CC denotes and
T14952 1534-1535 -RRB- denotes )
T14951 1532-1534 NN denotes µl
T14950 1528-1531 CD denotes 540
T14949 1527-1528 -LRB- denotes (
T14948 1518-1526 NN denotes solution
T14947 1509-1517 NN denotes labeling
T14946 1505-1508 DT denotes the
T14945 1502-1504 IN denotes of
T14944 1494-1501 NN denotes mixture
T14943 1492-1493 DT denotes a
T14942 1489-1491 IN denotes in
T14941 1479-1488 VB denotes incubated
T14940 1474-1478 VB denotes were
T14939 1465-1473 NN denotes sections
T14938 1447-1464 JJ denotes paraffin-embedded
T14937 1443-1446 DT denotes the
T14936 1441-1442 -COMMA- denotes ,
T14935 1434-1441 NN denotes tissues
T14934 1428-1433 NN denotes tumor
T14933 1424-1427 DT denotes the
T14932 1421-1423 IN denotes in
T14931 1415-1420 NN denotes death
T14930 1410-1414 NN denotes cell
T14929 1400-1409 JJ denotes apoptotic
T14928 1397-1399 IN denotes of
T14711 120-121 -RRB- denotes )
T14710 115-120 JJ denotes thick
T14709 112-114 NN denotes µm
T14708 110-111 CD denotes 4
T14707 109-110 -LRB- denotes (
T14706 100-108 NN denotes Sections
T14705 87-98 NN denotes examination
T14704 83-86 IN denotes for
T14703 74-82 NN denotes paraffin
T14702 71-73 IN denotes in
T14701 62-70 VB denotes embedded
T14700 58-61 CC denotes and
T14699 49-57 NN denotes formalin
T14698 46-48 IN denotes in
T14697 40-45 VB denotes fixed
T14696 35-39 VB denotes were
T14695 25-34 NN denotes specimens
T14694 21-24 DT denotes All
T14693 0-20 NN denotes Immunohistochemistry
R10220 T14695 T14694 arg1Of specimens,All
R10221 T14695 T14696 arg1Of specimens,were
R10222 T14695 T14697 arg2Of specimens,fixed
R10223 T14695 T14701 arg2Of specimens,embedded
R10224 T14697 T14698 arg1Of fixed,in
R10225 T14697 T14700 arg1Of fixed,and
R10226 T14699 T14698 arg2Of formalin,in
R10227 T14700 T14696 arg2Of and,were
R10228 T14701 T14700 arg2Of embedded,and
R10229 T14701 T14702 arg1Of embedded,in
R10230 T14703 T14702 arg2Of paraffin,in
R10231 T14703 T14704 arg1Of paraffin,for
R10232 T14705 T14704 arg2Of examination,for
R10233 T14706 T14707 arg1Of Sections,(
R10234 T14706 T14712 arg1Of Sections,were
R10235 T14706 T14713 arg2Of Sections,stained
R10236 T14706 T14719 arg2Of Sections,analyzed
R10237 T14709 T14707 arg2Of µm,(
R10238 T14709 T14708 arg1Of µm,4
R10239 T14709 T14710 arg1Of µm,thick
R10240 T14711 T14707 arg3Of ),(
R10241 T14713 T14714 arg1Of stained,with
R10242 T14713 T14718 arg1Of stained,and
R10243 T14717 T14714 arg2Of E,with
R10244 T14717 T14715 arg1Of E,H
R10245 T14717 T14716 arg1Of E,&
R10246 T14718 T14712 arg2Of and,were
R10247 T14719 T14718 arg2Of analyzed,and
R10248 T14721 T14719 arg1Of immunohistochemistry,analyzed
R10249 T14721 T14720 arg2Of immunohistochemistry,by
R10250 T14724 T14722 arg1Of sections,The
R10251 T14724 T14723 arg1Of sections,paraffin-embedded
R10252 T14724 T14725 arg1Of sections,were
R10253 T14724 T14726 arg2Of sections,deparaffinized
R10254 T14724 T14728 arg2Of sections,rehydrated
R10255 T14724 T14730 arg2Of sections,washed
R10256 T14724 T14737 arg2Of sections,subjected
R10257 T14726 T14727 arg1Of deparaffinized,and
R10258 T14727 T14729 arg1Of and,","
R10259 T14728 T14727 arg2Of rehydrated,and
R10260 T14729 T14735 arg1Of ",",and
R10261 T14730 T14729 arg2Of washed,","
R10262 T14730 T14731 arg1Of washed,in
R10263 T14733 T14731 arg2Of water,in
R10264 T14733 T14732 arg1Of water,distilled
R10265 T14735 T14725 arg2Of and,were
R10266 T14735 T14734 arg1Of and,","
R10267 T14737 T14735 arg2Of subjected,and
R10268 T14737 T14736 arg1Of subjected,then
R10269 T14737 T14738 arg1Of subjected,to
R10270 T14741 T14738 arg2Of retrieval,to
R10271 T14741 T14739 arg1Of retrieval,heat-mediated
R10272 T14741 T14740 arg1Of retrieval,antigen
R10273 T14744 T14742 arg1Of activity,Endogenous
R10274 T14744 T14743 arg1Of activity,peroxidase
R10275 T14744 T14745 arg1Of activity,was
R10276 T14744 T14746 arg2Of activity,quenched
R10277 T14746 T14745 arg2Of quenched,was
R10278 T14746 T14759 arg1Of quenched,","
R10279 T14746 T14760 modOf quenched,followed
R10280 T14748 T14746 arg1Of incubation,quenched
R10281 T14748 T14747 arg2Of incubation,by
R10282 T14748 T14749 arg1Of incubation,in
R10283 T14753 T14749 arg2Of peroxide,in
R10284 T14753 T14750 arg1Of peroxide,3
R10285 T14753 T14751 arg1Of peroxide,%
R10286 T14753 T14752 arg1Of peroxide,hydrogen
R10287 T14753 T14754 arg1Of peroxide,in
R10288 T14753 T14756 arg1Of peroxide,for
R10289 T14755 T14754 arg2Of methanol,in
R10290 T14758 T14756 arg2Of min,for
R10291 T14758 T14757 arg1Of min,15
R10292 T14760 T14761 arg1Of followed,by
R10293 T14762 T14761 arg2Of clearing,by
R10294 T14762 T14763 arg1Of clearing,in
R10295 T14762 T14765 arg1Of clearing,for
R10296 T14764 T14763 arg2Of PBS,in
R10297 T14767 T14765 arg2Of min,for
R10298 T14767 T14766 arg1Of min,5
R10299 T14769 T14768 arg1Of sections,The
R10300 T14769 T14770 arg1Of sections,were
R10301 T14769 T14771 arg2Of sections,blocked
R10302 T14769 T14788 arg2Of sections,incubated
R10303 T14771 T14772 arg1Of blocked,for
R10304 T14771 T14787 arg1Of blocked,and
R10305 T14774 T14772 arg2Of min,for
R10306 T14774 T14773 arg1Of min,30
R10307 T14774 T14775 arg1Of min,with
R10308 T14776 T14777 arg1Of 3,%
R10309 T14780 T14775 arg2Of serum,with
R10310 T14780 T14776 arg1Of serum,3
R10311 T14780 T14778 arg1Of serum,normal
R10312 T14780 T14779 arg1Of serum,horse
R10313 T14780 T14781 arg2Of serum,diluted
R10314 T14781 T14782 arg1Of diluted,in
R10315 T14783 T14782 arg2Of PBS,in
R10316 T14783 T14784 arg1Of PBS,","
R10317 T14783 T14785 arg2Of PBS,blotted
R10318 T14787 T14770 arg2Of and,were
R10319 T14787 T14786 arg1Of and,","
R10320 T14788 T14787 arg2Of incubated,and
R10321 T14788 T14789 arg1Of incubated,with
R10322 T14791 T14792 arg2Of mouse,anti-mouse
R10323 T14796 T14792 arg3Of antigen,anti-mouse
R10324 T14796 T14793 arg1Of antigen,proliferating
R10325 T14796 T14794 arg1Of antigen,cell
R10326 T14796 T14795 arg1Of antigen,nuclear
R10327 T14796 T14797 arg1Of antigen,(
R10328 T14798 T14797 arg2Of PCNA,(
R10329 T14798 T14799 arg1Of PCNA,","
R10330 T14801 T14799 arg2Of dilution,","
R10331 T14801 T14800 arg1Of dilution,1∶200
R10332 T14802 T14797 arg3Of ),(
R10333 T14804 T14789 arg2Of antibodies,with
R10334 T14804 T14790 arg1Of antibodies,primary
R10335 T14804 T14791 arg1Of antibodies,mouse
R10336 T14804 T14803 arg1Of antibodies,monoclonal
R10337 T14804 T14805 arg1Of antibodies,in
R10338 T14806 T14805 arg2Of blocking,in
R10339 T14806 T14808 arg1Of blocking,for
R10340 T14806 T14811 arg1Of blocking,at
R10341 T14807 T14806 arg2Of serum,blocking
R10342 T14810 T14808 arg2Of h,for
R10343 T14810 T14809 arg1Of h,4
R10344 T14813 T14811 arg2Of temperature,at
R10345 T14813 T14812 arg1Of temperature,room
R10346 T14817 T14816 arg1Of slides,the
R10347 T14817 T14818 arg1Of slides,were
R10348 T14817 T14819 arg2Of slides,washed
R10349 T14817 T14829 arg2Of slides,incubated
R10350 T14819 T14821 arg1Of washed,times
R10351 T14819 T14822 arg1Of washed,for
R10352 T14819 T14828 arg1Of washed,and
R10353 T14821 T14820 arg1Of times,three
R10354 T14824 T14822 arg2Of min,for
R10355 T14824 T14823 arg1Of min,5
R10356 T14824 T14825 arg1Of min,each
R10357 T14824 T14826 arg1Of min,in
R10358 T14827 T14826 arg2Of PBS,in
R10359 T14828 T14814 arg1Of and,Thereafter
R10360 T14828 T14815 arg1Of and,","
R10361 T14828 T14818 arg2Of and,were
R10362 T14829 T14828 arg2Of incubated,and
R10363 T14829 T14830 arg1Of incubated,with
R10364 T14832 T14833 arg1Of anti-mouse,and
R10365 T14834 T14833 arg2Of anti-rabbit,and
R10366 T14835 T14830 arg2Of antibodies,with
R10367 T14835 T14831 arg2Of antibodies,biotinylated
R10368 T14835 T14832 arg1Of antibodies,anti-mouse
R10369 T14835 T14834 arg1Of antibodies,anti-rabbit
R10370 T14835 T14836 arg1Of antibodies,for
R10371 T14838 T14836 arg2Of h,for
R10372 T14838 T14837 arg1Of h,2
R10373 T14840 T14839 arg1Of slides,The
R10374 T14840 T14841 arg1Of slides,were
R10375 T14840 T14842 arg2Of slides,washed
R10376 T14842 T14841 arg2Of washed,were
R10377 T14842 T14843 arg1Of washed,in
R10378 T14844 T14843 arg2Of PBS,in
R10379 T14844 T14845 arg1Of PBS,","
R10380 T14844 T14846 arg2Of PBS,followed
R10381 T14844 T14856 arg1Of PBS,(
R10382 T14849 T14846 arg1Of addition,followed
R10383 T14849 T14847 arg2Of addition,by
R10384 T14849 T14848 arg1Of addition,the
R10385 T14849 T14850 arg1Of addition,of
R10386 T14855 T14850 arg2Of complex,of
R10387 T14855 T14851 arg1Of complex,the
R10388 T14855 T14852 arg1Of complex,avidin
R10389 T14855 T14853 arg1Of complex,biotin
R10390 T14855 T14854 arg1Of complex,peroxidase
R10391 T14858 T14857 arg1Of kit,ABC
R10392 T14858 T14859 arg1Of kit,;
R10393 T14859 T14856 arg2Of ;,(
R10394 T14859 T14866 arg1Of ;,","
R10395 T14859 T14867 arg1Of ;,USA
R10396 T14861 T14860 arg1Of Laboratories,Vector
R10397 T14861 T14862 arg1Of Laboratories,","
R10398 T14862 T14864 arg1Of ",",","
R10399 T14863 T14862 arg2Of Burlingame,","
R10400 T14864 T14859 arg2Of ",",;
R10401 T14865 T14864 arg2Of CA,","
R10402 T14868 T14856 arg3Of ),(
R10403 T14870 T14869 arg1Of slides,The
R10404 T14870 T14871 arg1Of slides,were
R10405 T14870 T14872 arg2Of slides,washed
R10406 T14872 T14871 arg2Of washed,were
R10407 T14872 T14873 arg1Of washed,and
R10408 T14876 T14874 arg1Of reaction,the
R10409 T14876 T14875 arg1Of reaction,peroxidase
R10410 T14876 T14877 arg1Of reaction,was
R10411 T14876 T14878 arg2Of reaction,developed
R10412 T14878 T14873 arg2Of developed,and
R10413 T14878 T14877 arg2Of developed,was
R10414 T14878 T14879 arg1Of developed,with
R10415 T14878 T14886 arg1Of developed,","
R10416 T14878 T14887 modOf developed,followed
R10417 T14880 T14881 arg1Of diaminobenzidine,(
R10418 T14880 T14884 arg1Of diaminobenzidine,and
R10419 T14882 T14881 arg2Of DAB,(
R10420 T14883 T14881 arg3Of ),(
R10421 T14884 T14879 arg2Of and,with
R10422 T14885 T14884 arg2Of peroxide,and
R10423 T14889 T14890 arg1Of counterstaining,with
R10424 T14889 T14892 arg1Of counterstaining,","
R10425 T14889 T14893 modOf counterstaining,mounting
R10426 T14889 T14897 arg1Of counterstaining,and
R10427 T14891 T14890 arg2Of hematoxylin,with
R10428 T14893 T14894 arg1Of mounting,in
R10429 T14895 T14894 arg2Of aqua-mount,in
R10430 T14897 T14887 arg1Of and,followed
R10431 T14897 T14888 arg2Of and,by
R10432 T14897 T14896 arg1Of and,","
R10433 T14897 T14899 arg1Of and,under
R10434 T14898 T14897 arg2Of evaluation,and
R10435 T14902 T14899 arg2Of microscope,under
R10436 T14902 T14900 arg1Of microscope,a
R10437 T14902 T14901 arg1Of microscope,light
R10438 T14902 T14903 arg1Of microscope,(
R10439 T14904 T14903 arg2Of magnification×200,(
R10440 T14904 T14905 arg1Of magnification×200,;
R10441 T14906 T14905 arg2Of Olympus,;
R10442 T14906 T14907 arg1Of Olympus,","
R10443 T14908 T14907 arg2Of Tokyo,","
R10444 T14908 T14909 arg1Of Tokyo,","
R10445 T14910 T14909 arg2Of Japan,","
R10446 T14911 T14903 arg3Of ),(
R10447 T14914 T14912 arg1Of control,A
R10448 T14914 T14913 arg1Of control,negative
R10449 T14914 T14915 arg1Of control,was
R10450 T14914 T14916 arg2Of control,included
R10451 T14914 T14921 arg1Of control,omitting
R10452 T14916 T14915 arg2Of included,was
R10453 T14916 T14917 arg1Of included,in
R10454 T14916 T14920 arg1Of included,by
R10455 T14919 T14917 arg2Of experiments,in
R10456 T14919 T14918 arg1Of experiments,all
R10457 T14921 T14920 arg2Of omitting,by
R10458 T14924 T14921 arg2Of antibody,omitting
R10459 T14924 T14922 arg1Of antibody,the
R10460 T14924 T14923 arg1Of antibody,primary
R10461 T14927 T14925 arg2Of detection,For
R10462 T14927 T14926 arg1Of detection,the
R10463 T14927 T14928 arg1Of detection,of
R10464 T14927 T14932 arg1Of detection,in
R10465 T14931 T14928 arg2Of death,of
R10466 T14931 T14929 arg1Of death,apoptotic
R10467 T14931 T14930 arg1Of death,cell
R10468 T14935 T14932 arg2Of tissues,in
R10469 T14935 T14933 arg1Of tissues,the
R10470 T14935 T14934 arg1Of tissues,tumor
R10471 T14939 T14937 arg1Of sections,the
R10472 T14939 T14938 arg1Of sections,paraffin-embedded
R10473 T14939 T14940 arg1Of sections,were
R10474 T14939 T14941 arg2Of sections,incubated
R10475 T14939 T14968 arg1Of sections,washed
R10476 T14941 T14940 arg2Of incubated,were
R10477 T14941 T14942 arg1Of incubated,in
R10478 T14941 T14966 arg1Of incubated,and
R10479 T14944 T14945 arg1Of mixture,of
R10480 T14944 T14953 arg1Of mixture,and
R10481 T14948 T14945 arg2Of solution,of
R10482 T14948 T14946 arg1Of solution,the
R10483 T14948 T14947 arg1Of solution,labeling
R10484 T14948 T14949 arg1Of solution,(
R10485 T14951 T14949 arg2Of µl,(
R10486 T14951 T14950 arg1Of µl,540
R10487 T14952 T14949 arg3Of ),(
R10488 T14953 T14942 arg2Of and,in
R10489 T14953 T14943 arg1Of and,a
R10490 T14955 T14953 arg2Of solution,and
R10491 T14955 T14954 arg1Of solution,enzyme
R10492 T14955 T14956 arg1Of solution,(
R10493 T14955 T14960 arg1Of solution,for
R10494 T14958 T14956 arg2Of µl,(
R10495 T14958 T14957 arg1Of µl,60
R10496 T14959 T14956 arg3Of ),(
R10497 T14962 T14960 arg2Of h,for
R10498 T14962 T14961 arg1Of h,1
R10499 T14962 T14963 arg1Of h,at
R10500 T14964 T14963 arg2Of 37°C,at
R10501 T14966 T14925 arg1Of and,For
R10502 T14966 T14936 arg1Of and,","
R10503 T14966 T14965 arg1Of and,","
R10504 T14968 T14966 arg2Of washed,and
R10505 T14968 T14967 arg1Of washed,then
R10506 T14968 T14970 arg1Of washed,times
R10507 T14968 T14971 arg1Of washed,in
R10508 T14968 T14975 arg1Of washed,for
R10509 T14968 T14979 arg1Of washed,","
R10510 T14968 T14980 arg1Of washed,according
R10511 T14970 T14969 arg1Of times,three
R10512 T14972 T14973 arg1Of 0.1,M
R10513 T14974 T14971 arg2Of PBS,in
R10514 T14974 T14972 arg1Of PBS,0.1
R10515 T14977 T14975 arg2Of min,for
R10516 T14977 T14976 arg1Of min,5
R10517 T14978 T14968 arg2Of each,washed
R10518 T14981 T14980 arg2Of to,according
R10519 T14984 T14981 arg2Of instructions,to
R10520 T14984 T14982 arg1Of instructions,the
R10521 T14984 T14983 arg1Of instructions,manufacturer’s
R10522 T14988 T14987 arg1Of sections,the
R10523 T14988 T14989 arg1Of sections,were
R10524 T14988 T14990 arg2Of sections,incubated
R10525 T14988 T15010 arg2Of sections,mounted
R10526 T14988 T15015 arg1Of sections,cover-slipped
R10527 T14990 T14989 arg2Of incubated,were
R10528 T14990 T14991 arg1Of incubated,with
R10529 T14990 T14993 arg1Of incubated,","
R10530 T14992 T14991 arg2Of 4′,with
R10531 T14993 T14985 arg1Of ",",Next
R10532 T14993 T14986 arg1Of ",",","
R10533 T14993 T15010 modOf ",",mounted
R10534 T14993 T15015 modOf ",",cover-slipped
R10535 T14994 T14995 arg1Of 6-diamidino-2-phenylindole,(
R10536 T14994 T14998 arg1Of 6-diamidino-2-phenylindole,for
R10537 T14994 T15001 arg1Of 6-diamidino-2-phenylindole,at
R10538 T14994 T15007 arg1Of 6-diamidino-2-phenylindole,were
R10539 T14994 T15008 arg2Of 6-diamidino-2-phenylindole,rinsed
R10540 T14996 T14995 arg2Of DAPI,(
R10541 T14997 T14995 arg3Of ),(
R10542 T15000 T14998 arg2Of min,for
R10543 T15000 T14999 arg1Of min,15
R10544 T15003 T15001 arg2Of Finally,at
R10545 T15003 T15002 arg1Of Finally,37°C.
R10546 T15003 T15004 arg1Of Finally,","
R10547 T15006 T15004 arg2Of sections,","
R10548 T15006 T15005 arg1Of sections,the
R10549 T15008 T14993 arg2Of rinsed,","
R10550 T15008 T15007 arg2Of rinsed,were
R10551 T15008 T15009 arg1Of rinsed,","
R10552 T15010 T15011 arg1Of mounted,on
R10553 T15010 T15014 arg1Of mounted,and
R10554 T15012 T15011 arg2Of slides,on
R10555 T15014 T15013 arg1Of and,","
R10556 T15015 T15014 arg2Of cover-slipped,and
R10557 T15015 T15016 arg1Of cover-slipped,for
R10558 T15018 T15016 arg2Of microscopy,for
R10559 T15018 T15017 arg1Of microscopy,fluorescence
R10560 T15018 T15019 arg1Of microscopy,(
R10561 T15021 T15019 arg2Of microscope,(
R10562 T15021 T15020 arg1Of microscope,DAS
R10563 T15022 T15019 arg3Of ),(

UBERON-AE

Id Subject Object Predicate Lexical cue
T14659 1434-1441 http://purl.obolibrary.org/obo/UBERON_0000479 denotes tissues
T14658 701-706 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T14657 534-539 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum

GO-BP

Id Subject Object Predicate Lexical cue
T14678 1415-1420 http://purl.obolibrary.org/obo/GO_0016265 denotes death
T14677 1410-1420 http://purl.obolibrary.org/obo/GO_0008219 denotes cell death
T14676 1400-1420 http://purl.obolibrary.org/obo/GO_0006915 denotes apoptotic cell death
T14675 1387-1414 http://purl.obolibrary.org/obo/GO_0043654 denotes detection of apoptotic cell
T14674 1023-1025 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T14673 1064-1083 http://purl.obolibrary.org/obo/GO_0004601 denotes peroxidase reaction
T14672 342-361 http://purl.obolibrary.org/obo/GO_0004601 denotes peroxidase activity

GO-MF

Id Subject Object Predicate Lexical cue
T14692 1023-1025 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T14691 1369-1377 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T14690 864-874 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T14689 678-688 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T14688 1064-1083 http://purl.obolibrary.org/obo/GO_0004601 denotes peroxidase reaction
T14687 342-361 http://purl.obolibrary.org/obo/GO_0004601 denotes peroxidase activity

GO-CC

Id Subject Object Predicate Lexical cue
T14686 1369-1377 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T14685 864-874 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T14684 678-688 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T14683 1369-1377 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T14682 864-874 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T14681 678-688 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T14680 1410-1414 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T14679 623-627 http://purl.obolibrary.org/obo/GO_0005623 denotes cell

sentences

Id Subject Object Predicate Lexical cue
T14671 1684-1897 Sentence denotes Next, the sections were incubated with 4′,6-diamidino-2-phenylindole (DAPI) for 15 min at 37°C. Finally, the sections were rinsed, mounted on slides, and cover-slipped for fluorescence microscopy (DAS microscope).
T14670 1379-1683 Sentence denotes For the detection of apoptotic cell death in the tumor tissues, the paraffin-embedded sections were incubated in a mixture of the labeling solution (540 µl) and enzyme solution (60 µl) for 1 h at 37°C, and then washed three times in 0.1 M PBS for 5 min each, according to the manufacturer’s instructions.
T14669 1294-1378 Sentence denotes A negative control was included in all experiments by omitting the primary antibody.
T14668 1033-1293 Sentence denotes The slides were washed and the peroxidase reaction was developed with diaminobenzidine (DAB) and peroxide, followed by counterstaining with hematoxylin, mounting in aqua-mount, and evaluation under a light microscope (magnification×200; Olympus, Tokyo, Japan).
T14667 884-1032 Sentence denotes The slides were washed in PBS, followed by the addition of the avidin biotin peroxidase complex (ABC kit; Vector Laboratories, Burlingame, CA, USA).
T14666 736-883 Sentence denotes Thereafter, the slides were washed three times for 5 min each in PBS and incubated with biotinylated anti-mouse and anti-rabbit antibodies for 2 h.
T14665 476-735 Sentence denotes The sections were blocked for 30 min with 3% normal horse serum diluted in PBS, blotted, and incubated with primary mouse anti-mouse proliferating cell nuclear antigen (PCNA, 1∶200 dilution) monoclonal antibodies in blocking serum for 4 h at room temperature.
T14664 331-475 Sentence denotes Endogenous peroxidase activity was quenched by incubation in 3% hydrogen peroxide in methanol for 15 min, followed by clearing in PBS for 5 min.
T14663 182-330 Sentence denotes The paraffin-embedded sections were deparaffinized and rehydrated, washed in distilled water, and then subjected to heat-mediated antigen retrieval.
T14662 100-181 Sentence denotes Sections (4 µm thick) were stained with H&E and analyzed by immunohistochemistry.
T14661 21-99 Sentence denotes All specimens were fixed in formalin and embedded in paraffin for examination.
T14660 0-20 Sentence denotes Immunohistochemistry
T226 0-20 Sentence denotes Immunohistochemistry
T227 21-99 Sentence denotes All specimens were fixed in formalin and embedded in paraffin for examination.
T228 100-181 Sentence denotes Sections (4 µm thick) were stained with H&E and analyzed by immunohistochemistry.
T229 182-330 Sentence denotes The paraffin-embedded sections were deparaffinized and rehydrated, washed in distilled water, and then subjected to heat-mediated antigen retrieval.
T230 331-475 Sentence denotes Endogenous peroxidase activity was quenched by incubation in 3% hydrogen peroxide in methanol for 15 min, followed by clearing in PBS for 5 min.
T231 476-735 Sentence denotes The sections were blocked for 30 min with 3% normal horse serum diluted in PBS, blotted, and incubated with primary mouse anti-mouse proliferating cell nuclear antigen (PCNA, 1∶200 dilution) monoclonal antibodies in blocking serum for 4 h at room temperature.
T232 736-883 Sentence denotes Thereafter, the slides were washed three times for 5 min each in PBS and incubated with biotinylated anti-mouse and anti-rabbit antibodies for 2 h.
T233 884-1032 Sentence denotes The slides were washed in PBS, followed by the addition of the avidin biotin peroxidase complex (ABC kit; Vector Laboratories, Burlingame, CA, USA).
T234 1033-1293 Sentence denotes The slides were washed and the peroxidase reaction was developed with diaminobenzidine (DAB) and peroxide, followed by counterstaining with hematoxylin, mounting in aqua-mount, and evaluation under a light microscope (magnification×200; Olympus, Tokyo, Japan).
T235 1294-1378 Sentence denotes A negative control was included in all experiments by omitting the primary antibody.
T236 1379-1683 Sentence denotes For the detection of apoptotic cell death in the tumor tissues, the paraffin-embedded sections were incubated in a mixture of the labeling solution (540 µl) and enzyme solution (60 µl) for 1 h at 37°C, and then washed three times in 0.1 M PBS for 5 min each, according to the manufacturer’s instructions.
T237 1684-1779 Sentence denotes Next, the sections were incubated with 4′,6-diamidino-2-phenylindole (DAPI) for 15 min at 37°C.
T238 1780-1897 Sentence denotes Finally, the sections were rinsed, mounted on slides, and cover-slipped for fluorescence microscopy (DAS microscope).

bionlp-st-ge-2016-test-ihmc

Id Subject Object Predicate Lexical cue
T15412 1294-1377 Positive_regulation denotes A negative control was included in all experiments by omitting the primary antibody
T15411 280-329 Binding denotes then subjected to heat-mediated antigen retrieval
T15410 1540-1563 Entity denotes enzyme solution (60 µl)
T15409 49-57 Entity denotes formalin
T15408 657-665 Entity denotes dilution
T15407 940-979 Protein denotes of the avidin biotin peroxidase complex
T15406 628-650 Protein denotes nuclear antigen (PCNA,
T15405 667-688 Protein denotes monoclonal antibodies
T15404 940-979 Entity denotes of the avidin biotin peroxidase complex
T15403 1130-1139 Entity denotes peroxide,
T15402 1738-1759 Entity denotes 2-phenylindole (DAPI)
T15401 186-203 Entity denotes paraffin-embedded
T15400 711-714 Entity denotes 4 h
T15399 1540-1546 Protein denotes enzyme
T15398 312-319 Entity denotes antigen
T15397 1357-1377 Entity denotes the primary antibody
T15396 940-979 Entity denotes of the avidin biotin peroxidase complex
T15395 1173-1184 Entity denotes hematoxylin
T15394 1064-1074 Protein denotes peroxidase
T15393 1568-1580 Entity denotes 1 h at 37°C,
T15392 1754-1758 Entity denotes DAPI
T15391 74-82 Entity denotes paraffin
T15390 1492-1563 Entity denotes a mixture of the labeling solution (540 µl) and enzyme solution (60 µl)
T15389 1447-1464 Entity denotes paraffin-embedded
T15388 1881-1884 Entity denotes DAS
T15387 864-874 Entity denotes antibodies
T15386 342-352 Protein denotes peroxidase
T15385 623-656 Entity denotes cell nuclear antigen (PCNA, 1∶200
T15384 1505-1535 Entity denotes the labeling solution (540 µl)
T15383 961-971 Protein denotes peroxidase
T15382 645-649 Protein denotes PCNA
T15381 416-424 Entity denotes methanol
T15380 1198-1202 Entity denotes aqua
T15379 881-882 Entity denotes h
T15378 392-424 Entity denotes 3% hydrogen peroxide in methanol

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T15351 1764-1766 CD denotes 15
T15377 1896-1897 . denotes .
T15376 1895-1896 -RRB- denotes )
T15375 1885-1895 NN denotes microscope
T15374 1881-1884 NNP denotes DAS
T15373 1880-1881 -LRB- denotes (
T15372 1869-1879 NN denotes microscopy
T15371 1856-1868 NN denotes fluorescence
T15370 1852-1855 IN denotes for
T15369 1838-1851 JJ denotes cover-slipped
T15368 1834-1837 CC denotes and
T15367 1832-1833 , denotes ,
T15366 1826-1832 NNS denotes slides
T15365 1823-1825 IN denotes on
T15364 1815-1822 VBN denotes mounted
T15363 1813-1814 , denotes ,
T15362 1807-1813 VBN denotes rinsed
T15361 1802-1806 VBD denotes were
T15360 1793-1801 NNS denotes sections
T15359 1789-1792 DT denotes the
T15358 1787-1788 , denotes ,
T15357 1780-1787 RB denotes Finally
T15356 1777-1779 NNP denotes C.
T15355 1776-1777 CD denotes °
T15354 1774-1776 CD denotes 37
T15353 1771-1773 IN denotes at
T15352 1767-1770 NN denotes min
T15350 1760-1763 IN denotes for
T15349 1758-1759 -RRB- denotes )
T15348 1754-1758 NNP denotes DAPI
T15347 1753-1754 -LRB- denotes (
T15346 1728-1752 NN denotes diamidino-2-phenylindole
T15345 1727-1728 : denotes -
T15344 1725-1727 SYM denotes ,6
T15343 1724-1725 CD denotes ′
T15342 1723-1724 CD denotes 4
T15341 1718-1722 IN denotes with
T15340 1708-1717 VBN denotes incubated
T15339 1703-1707 VBD denotes were
T15338 1694-1702 NNS denotes sections
T15337 1690-1693 DT denotes the
T15336 1688-1689 , denotes ,
T15335 1684-1688 JJ denotes Next
T15334 1682-1683 . denotes .
T15333 1670-1682 NNS denotes instructions
T15332 1655-1667 NN denotes manufacturer
T15331 1651-1654 DT denotes the
T15330 1648-1650 TO denotes to
T15329 1638-1647 VBG denotes according
T15328 1636-1637 , denotes ,
T15327 1632-1636 DT denotes each
T15326 1628-1631 NN denotes min
T15325 1626-1627 CD denotes 5
T15324 1622-1625 IN denotes for
T15323 1618-1621 NNP denotes PBS
T15322 1616-1617 NNP denotes M
T15321 1612-1615 CD denotes 0.1
T15320 1609-1611 IN denotes in
T15319 1603-1608 NNS denotes times
T15318 1597-1602 CD denotes three
T15317 1590-1596 VBD denotes washed
T15316 1585-1589 RB denotes then
T15315 1581-1584 CC denotes and
T15314 1579-1580 , denotes ,
T15313 1578-1579 NNP denotes C
T15312 1577-1578 CD denotes °
T15311 1575-1577 CD denotes 37
T15310 1572-1574 IN denotes at
T15309 1570-1571 NN denotes h
T15308 1568-1569 CD denotes 1
T15307 1564-1567 IN denotes for
T15306 1562-1563 -RRB- denotes )
T15305 1560-1562 NN denotes µl
T15304 1557-1559 CD denotes 60
T15303 1556-1557 -LRB- denotes (
T15302 1547-1555 NN denotes solution
T15301 1540-1546 VB denotes enzyme
T15300 1536-1539 CC denotes and
T15299 1534-1535 -RRB- denotes )
T15298 1532-1534 NN denotes µl
T15297 1528-1531 CD denotes 540
T15296 1527-1528 -LRB- denotes (
T15295 1518-1526 NN denotes solution
T15294 1509-1517 VBG denotes labeling
T15293 1505-1508 DT denotes the
T15292 1502-1504 IN denotes of
T15291 1494-1501 NN denotes mixture
T15290 1492-1493 DT denotes a
T15289 1489-1491 IN denotes in
T15288 1479-1488 VBN denotes incubated
T15287 1474-1478 VBD denotes were
T15286 1465-1473 NNS denotes sections
T15285 1447-1464 JJ denotes paraffin-embedded
T15284 1443-1446 DT denotes the
T15283 1441-1442 , denotes ,
T15282 1434-1441 NNS denotes tissues
T15281 1428-1433 NN denotes tumor
T15280 1424-1427 DT denotes the
T15279 1421-1423 IN denotes in
T15278 1415-1420 NN denotes death
T15277 1410-1414 NN denotes cell
T15276 1400-1409 JJ denotes apoptotic
T15275 1397-1399 IN denotes of
T15274 1387-1396 NN denotes detection
T15273 1383-1386 DT denotes the
T15272 1379-1382 IN denotes For
T15271 1377-1378 . denotes .
T15270 1369-1377 NN denotes antibody
T15269 1361-1368 JJ denotes primary
T15268 1357-1360 DT denotes the
T15267 1348-1356 VBG denotes omitting
T15266 1345-1347 IN denotes by
T15265 1333-1344 NNS denotes experiments
T15264 1329-1332 DT denotes all
T15263 1326-1328 IN denotes in
T15262 1317-1325 VBN denotes included
T15261 1313-1316 VBD denotes was
T15260 1305-1312 NN denotes control
T15259 1296-1304 JJ denotes negative
T15258 1294-1295 DT denotes A
T15257 1292-1293 . denotes .
T15256 1291-1292 -RRB- denotes )
T15255 1286-1291 NNP denotes Japan
T15254 1284-1285 , denotes ,
T15253 1279-1284 NNP denotes Tokyo
T15252 1277-1278 , denotes ,
T15251 1270-1277 NNP denotes Olympus
T15250 1268-1269 : denotes ;
T15249 1265-1268 CD denotes 200
T15248 1264-1265 RB denotes ×
T15247 1251-1264 NN denotes magnification
T15117 528-533 NN denotes horse
T15116 521-527 JJ denotes normal
T15115 519-520 NN denotes %
T15114 518-519 CD denotes 3
T15113 513-517 IN denotes with
T15112 509-512 NN denotes min
T15111 506-508 CD denotes 30
T15110 502-505 IN denotes for
T15109 494-501 VBN denotes blocked
T15108 489-493 VBD denotes were
T15107 480-488 NNS denotes sections
T15106 476-479 DT denotes The
T15105 474-475 . denotes .
T15104 471-474 NN denotes min
T15103 469-470 CD denotes 5
T15102 465-468 IN denotes for
T15101 461-464 NNP denotes PBS
T15100 458-460 IN denotes in
T15099 449-457 VBG denotes clearing
T15098 446-448 IN denotes by
T15097 437-445 VBN denotes followed
T15096 435-436 , denotes ,
T15095 432-435 NN denotes min
T15094 429-431 CD denotes 15
T15093 425-428 IN denotes for
T15092 416-424 NN denotes methanol
T15091 413-415 IN denotes in
T15090 404-412 NN denotes peroxide
T15089 395-403 NN denotes hydrogen
T15088 393-394 NN denotes %
T15087 392-393 CD denotes 3
T15086 389-391 IN denotes in
T15085 378-388 NN denotes incubation
T15084 375-377 IN denotes by
T15083 366-374 VBN denotes quenched
T15082 362-365 VBD denotes was
T15081 353-361 NN denotes activity
T15080 342-352 NN denotes peroxidase
T15079 331-341 JJ denotes Endogenous
T15078 329-330 . denotes .
T15077 320-329 NN denotes retrieval
T15076 312-319 NN denotes antigen
T15075 298-311 JJ denotes heat-mediated
T15074 295-297 TO denotes to
T15073 285-294 VBN denotes subjected
T15072 280-284 RB denotes then
T15071 276-279 CC denotes and
T15070 274-275 , denotes ,
T15069 269-274 NN denotes water
T15068 259-268 JJ denotes distilled
T15067 256-258 IN denotes in
T15066 249-255 VBN denotes washed
T15065 247-248 , denotes ,
T15064 237-247 VBN denotes rehydrated
T15063 233-236 CC denotes and
T15062 218-232 VBN denotes deparaffinized
T15061 213-217 VBD denotes were
T15060 204-212 NNS denotes sections
T15059 186-203 JJ denotes paraffin-embedded
T15058 182-185 DT denotes The
T15057 180-181 . denotes .
T15056 160-180 NN denotes immunohistochemistry
T15055 157-159 IN denotes by
T15054 148-156 VBN denotes analyzed
T15053 144-147 CC denotes and
T15052 140-143 NNP denotes H&E
T15051 135-139 IN denotes with
T15050 127-134 VBN denotes stained
T15049 122-126 VBD denotes were
T15048 120-121 -RRB- denotes )
T15047 115-120 JJ denotes thick
T15046 112-114 NN denotes µm
T15045 110-111 CD denotes 4
T15044 109-110 -LRB- denotes (
T15043 100-108 NNS denotes Sections
T15042 98-99 . denotes .
T15041 87-98 NN denotes examination
T15040 83-86 IN denotes for
T15039 74-82 NN denotes paraffin
T15038 71-73 IN denotes in
T15037 62-70 VBN denotes embedded
T15036 58-61 CC denotes and
T15035 49-57 NN denotes formalin
T15034 46-48 IN denotes in
T15033 40-45 VBN denotes fixed
T15032 35-39 VBD denotes were
T15031 25-34 NNS denotes specimens
T15030 21-24 NNP denotes All
T15029 0-20 NNP denotes Immunohistochemistry
T15246 1250-1251 -LRB- denotes (
T15245 1239-1249 NN denotes microscope
T15244 1233-1238 JJ denotes light
T15243 1231-1232 DT denotes a
T15242 1225-1230 IN denotes under
T15241 1214-1224 NN denotes evaluation
T15240 1210-1213 CC denotes and
T15239 1208-1209 , denotes ,
T15238 1198-1208 JJ denotes aqua-mount
T15237 1195-1197 IN denotes in
T15236 1186-1194 VBG denotes mounting
T15235 1184-1185 , denotes ,
T15234 1173-1184 NN denotes hematoxylin
T15233 1168-1172 IN denotes with
T15232 1152-1167 VBG denotes counterstaining
T15231 1149-1151 IN denotes by
T15230 1140-1148 VBN denotes followed
T15229 1138-1139 , denotes ,
T15228 1130-1138 NN denotes peroxide
T15227 1126-1129 CC denotes and
T15226 1124-1125 -RRB- denotes )
T15225 1121-1124 NNP denotes DAB
T15224 1120-1121 -LRB- denotes (
T15199 981-984 NNP denotes ABC
T15198 980-981 -LRB- denotes (
T15197 972-979 NN denotes complex
T15196 961-971 NN denotes peroxidase
T15195 954-960 NN denotes biotin
T15194 947-953 NN denotes avidin
T15193 943-946 DT denotes the
T15192 940-942 IN denotes of
T15191 931-939 NN denotes addition
T15190 927-930 DT denotes the
T15189 924-926 IN denotes by
T15188 915-923 VBN denotes followed
T15187 913-914 , denotes ,
T15186 910-913 NNP denotes PBS
T15185 907-909 IN denotes in
T15184 900-906 VBN denotes washed
T15183 895-899 VBD denotes were
T15182 888-894 NNS denotes slides
T15181 884-887 DT denotes The
T15180 882-883 . denotes .
T15179 881-882 NN denotes h
T15178 879-880 CD denotes 2
T15177 875-878 IN denotes for
T15176 864-874 NNS denotes antibodies
T15175 852-863 JJ denotes anti-rabbit
T15174 848-851 CC denotes and
T15173 837-847 NN denotes anti-mouse
T15172 824-836 JJ denotes biotinylated
T15171 819-823 IN denotes with
T15170 809-818 VBN denotes incubated
T15169 805-808 CC denotes and
T15168 801-804 NNP denotes PBS
T15167 798-800 IN denotes in
T15166 793-797 DT denotes each
T15165 789-792 NN denotes min
T15164 787-788 CD denotes 5
T15163 783-786 IN denotes for
T15162 777-782 NNS denotes times
T15161 771-776 CD denotes three
T15160 764-770 VBN denotes washed
T15159 759-763 VBD denotes were
T15158 752-758 NNS denotes slides
T15157 748-751 DT denotes the
T15156 746-747 , denotes ,
T15155 736-746 RB denotes Thereafter
T15154 734-735 . denotes .
T15153 723-734 NN denotes temperature
T15152 718-722 NN denotes room
T15151 715-717 IN denotes at
T15150 713-714 NN denotes h
T15149 711-712 CD denotes 4
T15148 707-710 IN denotes for
T15147 701-706 NN denotes serum
T15146 692-700 VBG denotes blocking
T15145 689-691 IN denotes in
T15144 678-688 NNS denotes antibodies
T15143 667-677 JJ denotes monoclonal
T15142 665-666 -RRB- denotes )
T15141 657-665 NN denotes dilution
T15140 653-656 CD denotes 200
T15139 652-653 CD denotes ∶
T15138 651-652 CD denotes 1
T15137 649-650 , denotes ,
T15136 645-649 NNP denotes PCNA
T15135 644-645 -LRB- denotes (
T15134 636-643 NN denotes antigen
T15133 628-635 JJ denotes nuclear
T15132 623-627 NN denotes cell
T15131 609-622 VBG denotes proliferating
T15130 598-608 JJ denotes anti-mouse
T15129 592-597 NN denotes mouse
T15128 584-591 JJ denotes primary
T15127 579-583 IN denotes with
T15125 565-568 CC denotes and
T15124 563-564 , denotes ,
T15123 556-563 VBD denotes blotted
T15122 554-555 , denotes ,
T15121 551-554 NNP denotes PBS
T15120 548-550 IN denotes in
T15119 540-547 VBN denotes diluted
T15118 534-539 NN denotes serum
T15223 1103-1119 NN denotes diaminobenzidine
T15222 1098-1102 IN denotes with
T15221 1088-1097 VBN denotes developed
T15220 1084-1087 VBD denotes was
T15219 1075-1083 NN denotes reaction
T15218 1064-1074 JJ denotes peroxidase
T15217 1060-1063 DT denotes the
T15216 1056-1059 CC denotes and
T15215 1049-1055 VBN denotes washed
T15214 1044-1048 VBD denotes were
T15213 1037-1043 NNS denotes slides
T15212 1033-1036 DT denotes The
T15211 1031-1032 . denotes .
T15210 1030-1031 -RRB- denotes )
T15209 1027-1030 NNP denotes USA
T15208 1025-1026 , denotes ,
T15207 1023-1025 NNP denotes CA
T15206 1021-1022 , denotes ,
T15205 1011-1021 NNP denotes Burlingame
T15204 1009-1010 , denotes ,
T15203 997-1009 NNPS denotes Laboratories
T15202 990-996 NNP denotes Vector
T15201 988-989 : denotes ;
T15200 985-988 NN denotes kit
T15126 569-578 VBN denotes incubated
R10850 T15315 T15288 cc and,incubated
R10851 T15316 T15317 advmod then,washed
R10564 T15029 T15033 nsubjpass Immunohistochemistry,fixed
R10565 T15030 T15031 det All,specimens
R10566 T15031 T15033 nsubjpass specimens,fixed
R10567 T15032 T15033 auxpass were,fixed
R10568 T15033 T15033 ROOT fixed,fixed
R10569 T15034 T15033 prep in,fixed
R10570 T15035 T15034 pobj formalin,in
R10571 T15036 T15033 cc and,fixed
R10572 T15037 T15033 conj embedded,fixed
R10573 T15038 T15037 prep in,embedded
R10574 T15039 T15038 pobj paraffin,in
R10575 T15040 T15037 prep for,embedded
R10576 T15041 T15040 pobj examination,for
R10577 T15042 T15033 punct .,fixed
R10578 T15043 T15050 nsubjpass Sections,stained
R10579 T15044 T15043 punct (,Sections
R10580 T15045 T15046 nummod 4,µm
R10581 T15046 T15047 npadvmod µm,thick
R10582 T15047 T15043 appos thick,Sections
R10583 T15048 T15043 punct ),Sections
R10584 T15049 T15050 auxpass were,stained
R10585 T15050 T15050 ROOT stained,stained
R10586 T15051 T15050 prep with,stained
R10587 T15052 T15051 pobj H&E,with
R10588 T15053 T15050 cc and,stained
R10589 T15054 T15050 conj analyzed,stained
R10590 T15055 T15054 agent by,analyzed
R10591 T15056 T15055 pobj immunohistochemistry,by
R10592 T15057 T15050 punct .,stained
R10593 T15058 T15060 det The,sections
R10594 T15059 T15060 amod paraffin-embedded,sections
R10595 T15060 T15062 nsubjpass sections,deparaffinized
R10596 T15061 T15062 auxpass were,deparaffinized
R10597 T15062 T15062 ROOT deparaffinized,deparaffinized
R10598 T15063 T15062 cc and,deparaffinized
R10599 T15064 T15062 conj rehydrated,deparaffinized
R10600 T15065 T15064 punct ",",rehydrated
R10601 T15066 T15064 conj washed,rehydrated
R10602 T15067 T15066 prep in,washed
R10603 T15068 T15069 amod distilled,water
R10604 T15069 T15067 pobj water,in
R10605 T15070 T15066 punct ",",washed
R10606 T15071 T15066 cc and,washed
R10607 T15072 T15073 advmod then,subjected
R10608 T15073 T15066 conj subjected,washed
R10609 T15074 T15073 prep to,subjected
R10610 T15075 T15077 amod heat-mediated,retrieval
R10611 T15076 T15077 compound antigen,retrieval
R10612 T15077 T15074 pobj retrieval,to
R10613 T15078 T15062 punct .,deparaffinized
R10614 T15079 T15081 amod Endogenous,activity
R10615 T15080 T15081 compound peroxidase,activity
R10616 T15081 T15083 nsubjpass activity,quenched
R10617 T15082 T15083 auxpass was,quenched
R10618 T15083 T15083 ROOT quenched,quenched
R10619 T15084 T15083 agent by,quenched
R10620 T15085 T15084 pobj incubation,by
R10621 T15086 T15083 prep in,quenched
R10622 T15087 T15088 nummod 3,%
R10623 T15088 T15090 compound %,peroxide
R10624 T15089 T15090 compound hydrogen,peroxide
R10625 T15090 T15086 pobj peroxide,in
R10626 T15091 T15083 prep in,quenched
R10627 T15092 T15091 pobj methanol,in
R10628 T15093 T15083 prep for,quenched
R10629 T15094 T15095 nummod 15,min
R10630 T15095 T15093 pobj min,for
R10631 T15096 T15095 punct ",",min
R10632 T15097 T15083 advcl followed,quenched
R10633 T15098 T15097 prep by,followed
R10634 T15099 T15098 pcomp clearing,by
R10635 T15100 T15099 prep in,clearing
R10636 T15101 T15100 pobj PBS,in
R10637 T15102 T15099 prep for,clearing
R10638 T15103 T15104 nummod 5,min
R10639 T15104 T15102 pobj min,for
R10640 T15105 T15083 punct .,quenched
R10641 T15106 T15107 det The,sections
R10642 T15107 T15109 nsubjpass sections,blocked
R10643 T15108 T15109 auxpass were,blocked
R10644 T15109 T15109 ROOT blocked,blocked
R10645 T15110 T15109 prep for,blocked
R10646 T15111 T15112 nummod 30,min
R10647 T15112 T15110 pobj min,for
R10648 T15113 T15112 prep with,min
R10649 T15114 T15115 nummod 3,%
R10650 T15115 T15123 compound %,blotted
R10651 T15116 T15118 amod normal,serum
R10652 T15117 T15118 compound horse,serum
R10653 T15118 T15123 nsubj serum,blotted
R10654 T15119 T15118 acl diluted,serum
R10655 T15120 T15119 prep in,diluted
R10656 T15121 T15120 pobj PBS,in
R10657 T15122 T15118 punct ",",serum
R10658 T15123 T15109 advcl blotted,blocked
R10659 T15124 T15123 punct ",",blotted
R10660 T15125 T15123 cc and,blotted
R10661 T15126 T15123 conj incubated,blotted
R10662 T15127 T15126 prep with,incubated
R10663 T15128 T15129 amod primary,mouse
R10664 T15129 T15134 nmod mouse,antigen
R10665 T15130 T15134 amod anti-mouse,antigen
R10666 T15131 T15134 amod proliferating,antigen
R10667 T15132 T15134 compound cell,antigen
R10668 T15133 T15134 amod nuclear,antigen
R10669 T15134 T15127 pobj antigen,with
R10670 T15135 T15134 punct (,antigen
R10671 T15136 T15134 appos PCNA,antigen
R10672 T15137 T15136 punct ",",PCNA
R10673 T15138 T15140 nummod 1,200
R10674 T15139 T15140 nummod ∶,200
R10675 T15140 T15141 npadvmod 200,dilution
R10676 T15141 T15136 appos dilution,PCNA
R10677 T15142 T15136 punct ),PCNA
R10678 T15143 T15144 amod monoclonal,antibodies
R10679 T15144 T15134 appos antibodies,antigen
R10680 T15145 T15126 prep in,incubated
R10681 T15146 T15145 pcomp blocking,in
R10682 T15147 T15146 dobj serum,blocking
R10683 T15148 T15146 prep for,blocking
R10684 T15149 T15150 nummod 4,h
R10685 T15150 T15148 pobj h,for
R10686 T15151 T15150 prep at,h
R10687 T15152 T15153 compound room,temperature
R10688 T15153 T15151 pobj temperature,at
R10689 T15154 T15109 punct .,blocked
R10690 T15155 T15160 advmod Thereafter,washed
R10691 T15156 T15160 punct ",",washed
R10692 T15157 T15158 det the,slides
R10693 T15158 T15160 nsubjpass slides,washed
R10694 T15159 T15160 auxpass were,washed
R10695 T15160 T15160 ROOT washed,washed
R10696 T15161 T15162 nummod three,times
R10697 T15162 T15160 npadvmod times,washed
R10698 T15163 T15160 prep for,washed
R10699 T15164 T15165 nummod 5,min
R10700 T15165 T15163 pobj min,for
R10701 T15166 T15170 nsubj each,incubated
R10702 T15167 T15166 prep in,each
R10703 T15168 T15167 pobj PBS,in
R10704 T15169 T15166 cc and,each
R10705 T15170 T15160 advcl incubated,washed
R10706 T15171 T15170 prep with,incubated
R10707 T15172 T15173 amod biotinylated,anti-mouse
R10708 T15173 T15171 pobj anti-mouse,with
R10709 T15174 T15173 cc and,anti-mouse
R10710 T15175 T15176 amod anti-rabbit,antibodies
R10711 T15176 T15173 conj antibodies,anti-mouse
R10712 T15177 T15170 prep for,incubated
R10713 T15178 T15179 nummod 2,h
R10714 T15179 T15177 pobj h,for
R10715 T15180 T15160 punct .,washed
R10716 T15181 T15182 det The,slides
R10717 T15182 T15184 nsubjpass slides,washed
R10718 T15183 T15184 auxpass were,washed
R10719 T15184 T15203 ccomp washed,Laboratories
R10720 T15185 T15184 prep in,washed
R10721 T15186 T15185 pobj PBS,in
R10722 T15187 T15184 punct ",",washed
R10723 T15188 T15184 advcl followed,washed
R10724 T15189 T15188 agent by,followed
R10725 T15190 T15191 det the,addition
R10726 T15191 T15189 pobj addition,by
R10727 T15192 T15191 prep of,addition
R10728 T15193 T15197 det the,complex
R10729 T15194 T15197 nmod avidin,complex
R10730 T15195 T15197 nmod biotin,complex
R10731 T15196 T15197 compound peroxidase,complex
R10732 T15197 T15200 amod complex,kit
R10733 T15198 T15200 punct (,kit
R10734 T15199 T15200 compound ABC,kit
R10735 T15200 T15192 pobj kit,of
R10736 T15201 T15203 punct ;,Laboratories
R10737 T15202 T15203 compound Vector,Laboratories
R10738 T15203 T15203 ROOT Laboratories,Laboratories
R10739 T15204 T15203 punct ",",Laboratories
R10740 T15205 T15203 conj Burlingame,Laboratories
R10741 T15206 T15205 punct ",",Burlingame
R10742 T15207 T15205 conj CA,Burlingame
R10743 T15208 T15207 punct ",",CA
R10744 T15209 T15207 conj USA,CA
R10745 T15210 T15205 punct ),Burlingame
R10746 T15211 T15203 punct .,Laboratories
R10747 T15212 T15213 det The,slides
R10748 T15213 T15215 nsubjpass slides,washed
R10749 T15214 T15215 auxpass were,washed
R10750 T15215 T15215 ROOT washed,washed
R10751 T15216 T15215 cc and,washed
R10752 T15217 T15219 det the,reaction
R10753 T15218 T15219 amod peroxidase,reaction
R10754 T15219 T15221 nsubjpass reaction,developed
R10755 T15220 T15221 auxpass was,developed
R10756 T15221 T15215 conj developed,washed
R10757 T15222 T15221 prep with,developed
R10758 T15223 T15222 pobj diaminobenzidine,with
R10759 T15224 T15225 punct (,DAB
R10760 T15225 T15223 appos DAB,diaminobenzidine
R10761 T15226 T15225 punct ),DAB
R10762 T15227 T15223 cc and,diaminobenzidine
R10763 T15228 T15223 conj peroxide,diaminobenzidine
R10764 T15229 T15221 punct ",",developed
R10765 T15230 T15221 advcl followed,developed
R10766 T15231 T15221 agent by,developed
R10767 T15232 T15231 pcomp counterstaining,by
R10768 T15233 T15232 prep with,counterstaining
R10769 T15234 T15233 pobj hematoxylin,with
R10770 T15235 T15232 punct ",",counterstaining
R10771 T15236 T15232 conj mounting,counterstaining
R10772 T15237 T15236 prep in,mounting
R10773 T15238 T15237 pobj aqua-mount,in
R10774 T15239 T15236 punct ",",mounting
R10775 T15240 T15236 cc and,mounting
R10776 T15241 T15236 conj evaluation,mounting
R10777 T15242 T15241 prep under,evaluation
R10778 T15243 T15245 det a,microscope
R10779 T15244 T15245 amod light,microscope
R10780 T15245 T15242 pobj microscope,under
R10781 T15246 T15248 punct (,×
R10782 T15247 T15248 nsubj magnification,×
R10783 T15248 T15241 parataxis ×,evaluation
R10784 T15249 T15248 npadvmod 200,×
R10785 T15250 T15248 punct ;,×
R10786 T15251 T15248 conj Olympus,×
R10787 T15252 T15251 punct ",",Olympus
R10788 T15253 T15251 conj Tokyo,Olympus
R10789 T15254 T15253 punct ",",Tokyo
R10790 T15255 T15253 conj Japan,Tokyo
R10791 T15256 T15253 punct ),Tokyo
R10792 T15257 T15221 punct .,developed
R10793 T15258 T15260 det A,control
R10794 T15259 T15260 amod negative,control
R10795 T15260 T15262 nsubjpass control,included
R10796 T15261 T15262 auxpass was,included
R10797 T15262 T15262 ROOT included,included
R10798 T15263 T15262 prep in,included
R10799 T15264 T15265 det all,experiments
R10800 T15265 T15263 pobj experiments,in
R10801 T15266 T15262 prep by,included
R10802 T15267 T15266 pcomp omitting,by
R10803 T15268 T15270 det the,antibody
R10804 T15269 T15270 amod primary,antibody
R10805 T15270 T15267 dobj antibody,omitting
R10806 T15271 T15262 punct .,included
R10807 T15272 T15288 prep For,incubated
R10808 T15273 T15274 det the,detection
R10809 T15274 T15272 pobj detection,For
R10810 T15275 T15274 prep of,detection
R10811 T15276 T15278 amod apoptotic,death
R10812 T15277 T15278 compound cell,death
R10813 T15278 T15275 pobj death,of
R10814 T15279 T15274 prep in,detection
R10815 T15280 T15282 det the,tissues
R10816 T15281 T15282 compound tumor,tissues
R10817 T15282 T15279 pobj tissues,in
R10818 T15283 T15288 punct ",",incubated
R10819 T15284 T15286 det the,sections
R10820 T15285 T15286 amod paraffin-embedded,sections
R10821 T15286 T15288 nsubjpass sections,incubated
R10822 T15287 T15288 auxpass were,incubated
R10823 T15288 T15288 ROOT incubated,incubated
R10824 T15289 T15288 prep in,incubated
R10825 T15290 T15291 det a,mixture
R10826 T15291 T15289 pobj mixture,in
R10827 T15292 T15291 prep of,mixture
R10828 T15293 T15295 det the,solution
R10829 T15294 T15295 amod labeling,solution
R10830 T15295 T15292 pobj solution,of
R10831 T15296 T15295 punct (,solution
R10832 T15297 T15298 nummod 540,µl
R10833 T15298 T15295 appos µl,solution
R10834 T15299 T15295 punct ),solution
R10835 T15300 T15288 cc and,incubated
R10836 T15301 T15302 compound enzyme,solution
R10837 T15302 T15288 conj solution,incubated
R10838 T15303 T15305 punct (,µl
R10839 T15304 T15305 nummod 60,µl
R10840 T15305 T15302 appos µl,solution
R10841 T15306 T15302 punct ),solution
R10842 T15307 T15302 prep for,solution
R10843 T15308 T15309 nummod 1,h
R10844 T15309 T15307 pobj h,for
R10845 T15310 T15302 prep at,solution
R10846 T15311 T15312 nummod 37,°
R10847 T15312 T15310 pobj °,at
R10848 T15313 T15302 npadvmod C,solution
R10849 T15314 T15302 punct ",",solution
R10852 T15317 T15288 conj washed,incubated
R10853 T15318 T15319 nummod three,times
R10854 T15319 T15317 npadvmod times,washed
R10855 T15320 T15317 prep in,washed
R10856 T15321 T15323 nummod 0.1,PBS
R10857 T15322 T15323 compound M,PBS
R10858 T15323 T15320 pobj PBS,in
R10859 T15324 T15317 prep for,washed
R10860 T15325 T15326 nummod 5,min
R10861 T15326 T15324 pobj min,for
R10862 T15327 T15326 appos each,min
R10863 T15328 T15317 punct ",",washed
R10864 T15329 T15317 prep according,washed
R10865 T15330 T15329 prep to,according
R10866 T15331 T15332 det the,manufacturer
R10867 T15332 T15333 poss manufacturer,instructions
R10868 T15333 T15330 pobj instructions,to
R10869 T15334 T15288 punct .,incubated
R10870 T15335 T15340 advmod Next,incubated
R10871 T15336 T15340 punct ",",incubated
R10872 T15337 T15338 det the,sections
R10873 T15338 T15340 nsubjpass sections,incubated
R10874 T15339 T15340 auxpass were,incubated
R10875 T15340 T15340 ROOT incubated,incubated
R10876 T15341 T15340 prep with,incubated
R10877 T15342 T15343 nummod 4,′
R10878 T15343 T15341 pobj ′,with
R10879 T15344 T15346 punct ",6",diamidino-2-phenylindole
R10880 T15345 T15346 punct -,diamidino-2-phenylindole
R10881 T15346 T15346 ROOT diamidino-2-phenylindole,diamidino-2-phenylindole
R10882 T15347 T15348 punct (,DAPI
R10883 T15348 T15346 appos DAPI,diamidino-2-phenylindole
R10884 T15349 T15346 punct ),diamidino-2-phenylindole
R10885 T15350 T15346 prep for,diamidino-2-phenylindole
R10886 T15351 T15352 nummod 15,min
R10887 T15352 T15350 pobj min,for
R10888 T15353 T15346 prep at,diamidino-2-phenylindole
R10889 T15354 T15355 nummod 37,°
R10890 T15355 T15353 pobj °,at
R10891 T15356 T15353 pobj C.,at
R10892 T15357 T15362 advmod Finally,rinsed
R10893 T15358 T15362 punct ",",rinsed
R10894 T15359 T15360 det the,sections
R10895 T15360 T15362 nsubjpass sections,rinsed
R10896 T15361 T15362 auxpass were,rinsed
R10897 T15362 T15362 ROOT rinsed,rinsed
R10898 T15363 T15362 punct ",",rinsed
R10899 T15364 T15362 conj mounted,rinsed
R10900 T15365 T15364 prep on,mounted
R10901 T15366 T15365 pobj slides,on
R10902 T15367 T15365 punct ",",on
R10903 T15368 T15364 cc and,mounted
R10904 T15369 T15364 conj cover-slipped,mounted
R10905 T15370 T15369 prep for,cover-slipped
R10906 T15371 T15372 compound fluorescence,microscopy
R10907 T15372 T15370 pobj microscopy,for
R10908 T15373 T15375 punct (,microscope
R10909 T15374 T15375 compound DAS,microscope
R10910 T15375 T15372 appos microscope,microscopy
R10911 T15376 T15372 punct ),microscopy
R10912 T15377 T15362 punct .,rinsed

bionlp-st-ge-2016-test-tees

Id Subject Object Predicate Lexical cue
T15025 1064-1074 Protein denotes peroxidase
T15024 947-971 Protein denotes avidin biotin peroxidase
T15023 331-352 Protein denotes Endogenous peroxidase