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post-transfection, cells were lysed in lysis buffer of Dual Light Luciferase System (Tropix, Bedford, MA, USA) and the luciferase and β-galactosidase activities were evaluated by using Dual Light Luciferase System (Tropix) in a bioluminometer (Turner Biosystem, Sunnyvale, CA, USA). 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{"project":"GO-BP","denotations":[{"id":"T5504","span":{"begin":289,"end":291},"obj":"http://purl.obolibrary.org/obo/GO_0033968"},{"id":"T5508","span":{"begin":55,"end":60},"obj":"http://purl.obolibrary.org/obo/GO_0019835"},{"id":"T5510","span":{"begin":150,"end":176},"obj":"http://purl.obolibrary.org/obo/GO_0004565"},{"id":"T5511","span":{"begin":331,"end":358},"obj":"http://purl.obolibrary.org/obo/GO_0004565"},{"id":"T5512","span":{"begin":150,"end":176},"obj":"http://purl.obolibrary.org/obo/GO_0004336"},{"id":"T5513","span":{"begin":331,"end":358},"obj":"http://purl.obolibrary.org/obo/GO_0004336"},{"id":"T5514","span":{"begin":150,"end":176},"obj":"http://purl.obolibrary.org/obo/GO_1903771"},{"id":"T5515","span":{"begin":331,"end":358},"obj":"http://purl.obolibrary.org/obo/GO_1903771"},{"id":"T5516","span":{"begin":150,"end":176},"obj":"http://purl.obolibrary.org/obo/GO_1903770"},{"id":"T5517","span":{"begin":331,"end":358},"obj":"http://purl.obolibrary.org/obo/GO_1903770"},{"id":"T5518","span":{"begin":150,"end":176},"obj":"http://purl.obolibrary.org/obo/GO_0033920"},{"id":"T5519","span":{"begin":331,"end":358},"obj":"http://purl.obolibrary.org/obo/GO_0033920"},{"id":"T5520","span":{"begin":150,"end":176},"obj":"http://purl.obolibrary.org/obo/GO_0033929"},{"id":"T5521","span":{"begin":331,"end":358},"obj":"http://purl.obolibrary.org/obo/GO_0033929"},{"id":"T5522","span":{"begin":150,"end":176},"obj":"http://purl.obolibrary.org/obo/GO_0033930"},{"id":"T5523","span":{"begin":331,"end":358},"obj":"http://purl.obolibrary.org/obo/GO_0033930"},{"id":"T5524","span":{"begin":320,"end":339},"obj":"http://purl.obolibrary.org/obo/GO_0045289"},{"id":"T5525","span":{"begin":320,"end":339},"obj":"http://purl.obolibrary.org/obo/GO_0047077"},{"id":"T5526","span":{"begin":320,"end":339},"obj":"http://purl.obolibrary.org/obo/GO_0047712"},{"id":"T5527","span":{"begin":320,"end":339},"obj":"http://purl.obolibrary.org/obo/GO_0050248"},{"id":"T5528","span":{"begin":320,"end":339},"obj":"http://purl.obolibrary.org/obo/GO_0050397"},{"id":"T5972","span":{"begin":408,"end":424},"obj":"http://purl.obolibrary.org/obo/GO_0016246"}],"text":"orty-eight-hour post-transfection, cells were lysed in lysis buffer of Dual Light Luciferase System (Tropix, Bedford, MA, USA) and the luciferase and β-galactosidase activities were evaluated by using Dual Light Luciferase System (Tropix) in a bioluminometer (Turner Biosystem, Sunnyvale, CA, USA). The ratio of firefly luciferase activity to β-galactosidase activity was expressed as relative light units.\n\nRNA interference\nJurkat or U937 cells "}

{"project":"GO-MF","denotations":[{"id":"T5530","span":{"begin":289,"end":291},"obj":"http://purl.obolibrary.org/obo/GO_0033968"},{"id":"T5531","span":{"begin":150,"end":176},"obj":"http://purl.obolibrary.org/obo/GO_0004565"},{"id":"T5532","span":{"begin":331,"end":358},"obj":"http://purl.obolibrary.org/obo/GO_0004565"},{"id":"T5533","span":{"begin":150,"end":176},"obj":"http://purl.obolibrary.org/obo/GO_0004336"},{"id":"T5534","span":{"begin":331,"end":358},"obj":"http://purl.obolibrary.org/obo/GO_0004336"},{"id":"T5535","span":{"begin":150,"end":176},"obj":"http://purl.obolibrary.org/obo/GO_0033920"},{"id":"T5536","span":{"begin":331,"end":358},"obj":"http://purl.obolibrary.org/obo/GO_0033920"},{"id":"T5537","span":{"begin":150,"end":176},"obj":"http://purl.obolibrary.org/obo/GO_0033929"},{"id":"T5538","span":{"begin":331,"end":358},"obj":"http://purl.obolibrary.org/obo/GO_0033929"},{"id":"T5539","span":{"begin":150,"end":176},"obj":"http://purl.obolibrary.org/obo/GO_0033930"},{"id":"T5540","span":{"begin":331,"end":358},"obj":"http://purl.obolibrary.org/obo/GO_0033930"},{"id":"T5541","span":{"begin":320,"end":339},"obj":"http://purl.obolibrary.org/obo/GO_0045289"},{"id":"T5542","span":{"begin":320,"end":339},"obj":"http://purl.obolibrary.org/obo/GO_0047077"},{"id":"T5543","span":{"begin":320,"end":339},"obj":"http://purl.obolibrary.org/obo/GO_0047712"},{"id":"T5544","span":{"begin":320,"end":339},"obj":"http://purl.obolibrary.org/obo/GO_0050248"},{"id":"T5545","span":{"begin":320,"end":339},"obj":"http://purl.obolibrary.org/obo/GO_0050397"}],"text":"orty-eight-hour post-transfection, cells were lysed in lysis buffer of Dual Light Luciferase System (Tropix, Bedford, MA, USA) and the luciferase and β-galactosidase activities were evaluated by using Dual Light Luciferase System (Tropix) in a bioluminometer (Turner Biosystem, Sunnyvale, CA, USA). The ratio of firefly luciferase activity to β-galactosidase activity was expressed as relative light units.\n\nRNA interference\nJurkat or U937 cells "}

{"project":"GO-CC","denotations":[{"id":"T5550","span":{"begin":35,"end":40},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T5969","span":{"begin":440,"end":445},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"orty-eight-hour post-transfection, cells were lysed in lysis buffer of Dual Light Luciferase System (Tropix, Bedford, MA, USA) and the luciferase and β-galactosidase activities were evaluated by using Dual Light Luciferase System (Tropix) in a bioluminometer (Turner Biosystem, Sunnyvale, CA, USA). The ratio of firefly luciferase activity to β-galactosidase activity was expressed as relative light units.\n\nRNA interference\nJurkat or U937 cells "}

{"project":"sentences","denotations":[{"id":"T4914","span":{"begin":299,"end":406},"obj":"Sentence"},{"id":"T5677","span":{"begin":408,"end":424},"obj":"Sentence"},{"id":"T49","span":{"begin":299,"end":406},"obj":"Sentence"},{"id":"T50","span":{"begin":408,"end":424},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"orty-eight-hour post-transfection, cells were lysed in lysis buffer of Dual Light Luciferase System (Tropix, Bedford, MA, USA) and the luciferase and β-galactosidase activities were evaluated by using Dual Light Luciferase System (Tropix) in a bioluminometer (Turner Biosystem, Sunnyvale, CA, USA). The ratio of firefly luciferase activity to β-galactosidase activity was expressed as relative light units.\n\nRNA interference\nJurkat or U937 cells "}

{"project":"events-check-again","denotations":[{"id":"T5631","span":{"begin":82,"end":92},"obj":"Protein"},{"id":"T5632","span":{"begin":135,"end":145},"obj":"Protein"},{"id":"T5633","span":{"begin":150,"end":165},"obj":"Protein"},{"id":"T5634","span":{"begin":320,"end":330},"obj":"Protein"},{"id":"T5635","span":{"begin":343,"end":358},"obj":"Protein"}],"text":"orty-eight-hour post-transfection, cells were lysed in lysis buffer of Dual Light Luciferase System (Tropix, Bedford, MA, USA) and the luciferase and β-galactosidase activities were evaluated by using Dual Light Luciferase System (Tropix) in a bioluminometer (Turner Biosystem, Sunnyvale, CA, USA). The ratio of firefly luciferase activity to β-galactosidase activity was expressed as relative light units.\n\nRNA interference\nJurkat or U937 cells "}

{"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T5647","span":{"begin":76,"end":92},"obj":"Protein"},{"id":"T5648","span":{"begin":135,"end":145},"obj":"Protein"},{"id":"T5649","span":{"begin":150,"end":165},"obj":"Protein"},{"id":"T5650","span":{"begin":206,"end":222},"obj":"Protein"},{"id":"T5651","span":{"begin":320,"end":330},"obj":"Protein"},{"id":"T5652","span":{"begin":343,"end":358},"obj":"Protein"}],"text":"orty-eight-hour post-transfection, cells were lysed in lysis buffer of Dual Light Luciferase System (Tropix, Bedford, MA, USA) and the luciferase and β-galactosidase activities were evaluated by using Dual Light Luciferase System (Tropix) in a bioluminometer (Turner Biosystem, Sunnyvale, CA, USA). The ratio of firefly luciferase activity to β-galactosidase activity was expressed as relative light units.\n\nRNA interference\nJurkat or U937 cells "}

{"project":"test2","denotations":[{"id":"T4884","span":{"begin":82,"end":92},"obj":"Protein"},{"id":"T4885","span":{"begin":135,"end":145},"obj":"Protein"},{"id":"T4886","span":{"begin":150,"end":165},"obj":"Protein"},{"id":"T4887","span":{"begin":320,"end":330},"obj":"Protein"},{"id":"T4888","span":{"begin":343,"end":358},"obj":"Protein"},{"id":"T4889","span":{"begin":372,"end":381},"obj":"Gene_expression"}],"text":"orty-eight-hour post-transfection, cells were lysed in lysis buffer of Dual Light Luciferase System (Tropix, Bedford, MA, USA) and the luciferase and β-galactosidase activities were evaluated by using Dual Light Luciferase System (Tropix) in a bioluminometer (Turner Biosystem, Sunnyvale, CA, USA). The ratio of firefly luciferase activity to β-galactosidase activity was expressed as relative light units.\n\nRNA interference\nJurkat or U937 cells "}

{"project":"bionlp-st-ge-2016-reference","denotations":[{"id":"T4901","span":{"begin":82,"end":92},"obj":"Protein"},{"id":"T4902","span":{"begin":135,"end":145},"obj":"Protein"},{"id":"T4903","span":{"begin":150,"end":165},"obj":"Protein"},{"id":"T4904","span":{"begin":320,"end":330},"obj":"Protein"},{"id":"T4905","span":{"begin":343,"end":358},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"orty-eight-hour post-transfection, cells were lysed in lysis buffer of Dual Light Luciferase System (Tropix, Bedford, MA, USA) and the luciferase and β-galactosidase activities were evaluated by using Dual Light Luciferase System (Tropix) in a bioluminometer (Turner Biosystem, Sunnyvale, CA, USA). The ratio of firefly luciferase activity to β-galactosidase activity was expressed as relative light units.\n\nRNA interference\nJurkat or U937 cells "}

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T5194","span":{"begin":82,"end":92},"obj":"P08659"},{"id":"T5195","span":{"begin":135,"end":145},"obj":"P08659"},{"id":"T5196","span":{"begin":212,"end":222},"obj":"P08659"},{"id":"T5197","span":{"begin":320,"end":330},"obj":"P08659"},{"id":"T5198","span":{"begin":385,"end":393},"obj":"Q04864"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"orty-eight-hour post-transfection, cells were lysed in lysis buffer of Dual Light Luciferase System (Tropix, Bedford, MA, USA) and the luciferase and β-galactosidase activities were evaluated by using Dual Light Luciferase System (Tropix) in a bioluminometer (Turner Biosystem, Sunnyvale, CA, USA). The ratio of firefly luciferase activity to β-galactosidase activity was expressed as relative light units.\n\nRNA interference\nJurkat or U937 cells "}