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PMC:3333881 / 20559-21007 JSONTXT

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pmc-enju-pas

Id Subject Object Predicate Lexical cue
T24419 0-7 JJ denotes Nuclear
T24420 8-11 CC denotes and
T24421 12-21 JJ denotes cytosolic
T24422 22-30 NN denotes extracts
T24423 31-35 VB denotes were
T24424 36-44 VB denotes prepared
T24425 45-48 IN denotes for
T24426 49-56 JJ denotes further
T24427 57-66 NN denotes analysis.
T24428 67-68 -LRB- denotes (
T24429 68-69 NN denotes A
T24430 69-70 -RRB- denotes )
T24431 71-78 JJ denotes Nuclear
T24432 79-87 NN denotes extracts
T24433 88-92 VB denotes were
T24434 93-101 VB denotes analysed
T24435 102-105 IN denotes for
T24436 106-109 DT denotes the
T24437 110-113 NN denotes p65
T24438 113-114 -COMMA- denotes ,
T24439 115-118 NN denotes p50
T24440 119-122 CC denotes and
T24441 123-131 NN denotes FLAG-Tat
T24442 132-139 NN denotes binding
T24443 140-142 TO denotes to
T24444 143-146 DT denotes the
T24445 147-152 NN denotes NF-κB
T24446 153-168 JJ denotes double-stranded
T24447 169-184 NN denotes oligonucleotide
T24448 185-190 VB denotes using
T24449 191-194 DT denotes the
T24450 195-200 NN denotes NF-κB
T24451 201-214 NN denotes Transcription
T24452 215-221 NN denotes Factor
T24453 222-227 NN denotes ELISA
T24454 228-233 NN denotes assay
T24455 234-237 NN denotes kit
T24456 238-239 -LRB- denotes (
T24457 239-245 NN denotes Cayman
T24458 245-246 -RRB- denotes )
T24459 246-247 -LRB- denotes .
T24460 248-249 NN denotes (
T24461 249-250 -RRB- denotes B
T24462 250-257 JJ denotes ) Nucle
T24463 257-260 CC denotes ar
T24464 260-269 JJ denotes and cytos
T24465 269-277 NN denotes olic ext
T24466 277-278 -LRB- denotes r
T24467 278-283 NN denotes acts
T24468 283-284 -RRB- denotes (
T24469 284-288 VB denotes 20 µ
T24470 288-297 VB denotes g) were s
T24471 297-299 IN denotes ep
T24472 299-301 CD denotes ar
T24473 301-302 NN denotes a
T24474 302-310 NN denotes ted by 1
T24475 310-313 CC denotes 2%
T24476 313-321 VB denotes SDS–PAGE
T24477 322-324 IN denotes an
T24478 324-331 JJ denotes d analy
T24479 331-339 NN denotes sed by w
T24480 339-344 VB denotes ester
T24481 344-347 DT denotes n b
T24482 347-355 JJ denotes lotting
T24483 355-356 -COMMA- denotes u
T24484 356-365 JJ denotes sing the
T24485 365-366 -COMMA- denotes a
T24486 366-378 JJ denotes nti-p65, ant
T24487 378-380 NN denotes i-
T24488 380-381 -COMMA- denotes F
T24489 381-391 JJ denotes LAG, anti-
T24490 391-394 CC denotes His
T24491 394-412 JJ denotes tone H1, anti-IκB-
T24492 412-422 NN denotes α and anti
R19419 T24419 T24420 arg1Of Nuclear,and
R19420 T24421 T24420 arg2Of cytosolic,and
R19421 T24422 T24419 arg1Of extracts,Nuclear
R19422 T24422 T24421 arg1Of extracts,cytosolic
R19423 T24422 T24423 arg1Of extracts,were
R19424 T24422 T24424 arg2Of extracts,prepared
R19425 T24424 T24423 arg2Of prepared,were
R19426 T24424 T24425 arg1Of prepared,for
R19427 T24427 T24425 arg2Of analysis.,for
R19428 T24427 T24426 arg1Of analysis.,further
R19429 T24427 T24433 modOf analysis.,were
R19430 T24429 T24428 arg2Of A,(
R19431 T24430 T24428 arg3Of ),(
R19432 T24432 T24428 arg1Of extracts,(
R19433 T24432 T24431 arg1Of extracts,Nuclear
R19434 T24432 T24433 arg1Of extracts,were
R19435 T24432 T24434 arg2Of extracts,analysed
R19436 T24434 T24433 arg2Of analysed,were
R19437 T24434 T24435 arg1Of analysed,for
R19438 T24437 T24438 arg1Of p65,","
R19439 T24438 T24440 arg1Of ",",and
R19440 T24439 T24438 arg2Of p50,","
R19441 T24441 T24440 arg2Of FLAG-Tat,and
R19442 T24442 T24435 arg2Of binding,for
R19443 T24442 T24436 arg1Of binding,the
R19444 T24442 T24437 arg1Of binding,p65
R19445 T24442 T24439 arg1Of binding,p50
R19446 T24442 T24441 arg1Of binding,FLAG-Tat
R19447 T24442 T24443 arg1Of binding,to
R19448 T24447 T24443 arg2Of oligonucleotide,to
R19449 T24447 T24444 arg1Of oligonucleotide,the
R19450 T24447 T24445 arg1Of oligonucleotide,NF-κB
R19451 T24447 T24446 arg1Of oligonucleotide,double-stranded
R19452 T24447 T24448 arg1Of oligonucleotide,using
R19453 T24455 T24449 arg1Of kit,the
R19454 T24455 T24450 arg1Of kit,NF-κB
R19455 T24455 T24451 arg1Of kit,Transcription
R19456 T24455 T24452 arg1Of kit,Factor
R19457 T24455 T24453 arg1Of kit,ELISA
R19458 T24455 T24454 arg1Of kit,assay
R19459 T24455 T24456 arg1Of kit,(
R19460 T24455 T24469 modOf kit,20 µ
R19461 T24455 T24480 arg1Of kit,ester
R19462 T24455 T24488 arg1Of kit,F
R19463 T24457 T24456 arg2Of Cayman,(
R19464 T24458 T24456 arg3Of ),(
R19465 T24460 T24459 arg2Of (,.
R19466 T24461 T24459 arg3Of B,.
R19467 T24462 T24463 arg1Of ) Nucle,ar
R19468 T24464 T24463 arg2Of and cytos,ar
R19469 T24465 T24459 arg1Of olic ext,.
R19470 T24465 T24462 arg1Of olic ext,) Nucle
R19471 T24465 T24464 arg1Of olic ext,and cytos
R19472 T24465 T24466 arg1Of olic ext,r
R19473 T24465 T24469 arg1Of olic ext,20 µ
R19474 T24465 T24470 arg2Of olic ext,g) were s
R19475 T24465 T24476 arg2Of olic ext,SDS–PAGE
R19476 T24467 T24466 arg2Of acts ,r
R19477 T24468 T24466 arg3Of (,r
R19478 T24470 T24475 arg1Of g) were s,2%
R19479 T24472 T24473 arg1Of ar,a
R19480 T24474 T24470 arg1Of ted by 1,g) were s
R19481 T24474 T24471 arg2Of ted by 1,ep
R19482 T24474 T24472 arg1Of ted by 1,ar
R19483 T24475 T24469 arg2Of 2% ,20 µ
R19484 T24476 T24475 arg2Of SDS–PAGE,2%
R19485 T24479 T24476 arg1Of sed by w,SDS–PAGE
R19486 T24479 T24477 arg2Of sed by w,an
R19487 T24479 T24478 arg1Of sed by w,d analy
R19488 T24482 T24483 arg1Of lotting ,u
R19489 T24483 T24485 arg1Of u,a
R19490 T24484 T24483 arg2Of sing the ,u
R19491 T24486 T24485 arg2Of "nti-p65, ant",a
R19492 T24487 T24480 arg2Of i-,ester
R19493 T24487 T24481 arg1Of i-,n b
R19494 T24487 T24482 arg1Of i-,lotting
R19495 T24487 T24484 arg1Of i-,sing the
R19496 T24487 T24486 arg1Of i-,"nti-p65, ant"
R19497 T24488 T24448 arg2Of F,using
R19498 T24489 T24490 arg1Of "LAG, anti-",His
R19499 T24491 T24490 arg2Of "tone H1, anti-IκB-",His
R19500 T24492 T24488 arg2Of α and anti,F
R19501 T24492 T24489 arg1Of α and anti,"LAG, anti-"
R19502 T24492 T24491 arg1Of α and anti,"tone H1, anti-IκB-"

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T24740 0-7 NNP denotes Nuclear
T24741 8-11 CC denotes and
T24742 12-21 JJ denotes cytosolic
T24743 22-30 NNS denotes extracts
T24744 31-35 VBD denotes were
T24745 36-44 VBN denotes prepared
T24746 45-48 IN denotes for
T24747 49-56 JJ denotes further
T24748 57-65 NN denotes analysis
T24749 65-66 . denotes .
T24750 67-68 -LRB- denotes (
T24751 68-69 NNP denotes A
T24752 69-70 -RRB- denotes )
T24753 71-78 NNP denotes Nuclear
T24754 79-87 NNS denotes extracts
T24755 88-92 VBD denotes were
T24756 93-101 VBN denotes analysed
T24757 102-105 IN denotes for
T24758 106-109 DT denotes the
T24759 110-113 NNS denotes p65
T24760 113-114 , denotes ,
T24761 115-118 JJ denotes p50
T24762 119-122 CC denotes and
T24763 123-131 JJ denotes FLAG-Tat
T24764 132-139 JJ denotes binding
T24765 140-142 TO denotes to
T24766 143-146 DT denotes the
T24767 147-152 NNP denotes NF-κB
T24768 153-168 JJ denotes double-stranded
T24769 169-184 NN denotes oligonucleotide
T24770 185-190 VBG denotes using
T24771 191-194 DT denotes the
T24772 195-200 NNP denotes NF-κB
T24773 201-214 NNP denotes Transcription
T24774 215-221 NNP denotes Factor
T24775 222-227 NNP denotes ELISA
T24776 228-233 NN denotes assay
T24777 234-237 NN denotes kit
T24778 238-239 -LRB- denotes (
T24779 239-245 NNP denotes Cayman
T24780 245-246 -RRB- denotes )
T24781 246-247 . denotes .
T24782 248-249 -LRB- denotes (
T24783 249-250 NNP denotes B
T24784 250-251 -RRB- denotes )
T24785 252-259 NNP denotes Nuclear
T24786 260-263 CC denotes and
T24787 264-273 JJ denotes cytosolic
T24788 274-282 NNS denotes extracts
T24789 283-284 -LRB- denotes (
T24790 284-286 CD denotes 20
T24791 287-289 NN denotes µg
T24792 289-290 -RRB- denotes )
T24793 291-295 VBD denotes were
T24794 296-305 VBN denotes separated
T24795 306-308 IN denotes by
T24796 309-311 CD denotes 12
T24797 311-312 NN denotes %
T24798 313-316 NNP denotes SDS
T24799 317-321 NNP denotes PAGE
T24800 322-325 CC denotes and
T24801 326-334 VBN denotes analysed
T24802 335-337 IN denotes by
T24803 338-345 JJ denotes western
T24804 346-354 VBG denotes blotting
T24805 355-360 VBG denotes using
T24806 361-364 DT denotes the
T24807 365-373 JJ denotes anti-p65
T24808 373-374 , denotes ,
T24809 375-384 JJ denotes anti-FLAG
T24810 384-385 , denotes ,
T24811 386-398 JJ denotes anti-Histone
T24812 399-401 NN denotes H1
T24813 401-402 , denotes ,
T24814 403-413 JJ denotes anti-IκB-α
T24815 414-417 CC denotes and
T24816 418-436 JJ denotes anti-Hexokinase-II
T24817 437-447 NNS denotes antibodies
T24818 447-448 . denotes .
R19730 T24740 T24743 amod Nuclear,extracts
R19731 T24741 T24740 cc and,Nuclear
R19732 T24742 T24740 conj cytosolic,Nuclear
R19733 T24743 T24745 nsubjpass extracts,prepared
R19734 T24744 T24745 auxpass were,prepared
R19735 T24745 T24745 ROOT prepared,prepared
R19736 T24746 T24745 prep for,prepared
R19737 T24747 T24748 amod further,analysis
R19738 T24748 T24746 pobj analysis,for
R19739 T24749 T24745 punct .,prepared
R19740 T24750 T24756 punct (,analysed
R19741 T24751 T24754 nmod A,extracts
R19742 T24752 T24751 punct ),A
R19743 T24753 T24754 compound Nuclear,extracts
R19744 T24754 T24756 nsubjpass extracts,analysed
R19745 T24755 T24756 auxpass were,analysed
R19746 T24756 T24756 ROOT analysed,analysed
R19747 T24757 T24756 prep for,analysed
R19748 T24758 T24759 det the,p65
R19749 T24759 T24757 pobj p65,for
R19750 T24760 T24759 punct ",",p65
R19751 T24761 T24759 amod p50,p65
R19752 T24762 T24761 cc and,p50
R19753 T24763 T24761 conj FLAG-Tat,p50
R19754 T24764 T24756 oprd binding,analysed
R19755 T24765 T24764 prep to,binding
R19756 T24766 T24769 det the,oligonucleotide
R19757 T24767 T24769 nmod NF-κB,oligonucleotide
R19758 T24768 T24769 amod double-stranded,oligonucleotide
R19759 T24769 T24765 pobj oligonucleotide,to
R19760 T24770 T24756 advcl using,analysed
R19761 T24771 T24777 det the,kit
R19762 T24772 T24775 compound NF-κB,ELISA
R19763 T24773 T24775 compound Transcription,ELISA
R19764 T24774 T24775 compound Factor,ELISA
R19765 T24775 T24777 compound ELISA,kit
R19766 T24776 T24777 compound assay,kit
R19767 T24777 T24770 dobj kit,using
R19768 T24778 T24777 punct (,kit
R19769 T24779 T24777 appos Cayman,kit
R19770 T24780 T24777 punct ),kit
R19771 T24781 T24756 punct .,analysed
R19772 T24782 T24785 punct (,Nuclear
R19773 T24783 T24785 nmod B,Nuclear
R19774 T24784 T24785 punct ),Nuclear
R19775 T24785 T24788 nmod Nuclear,extracts
R19776 T24786 T24785 cc and,Nuclear
R19777 T24787 T24785 conj cytosolic,Nuclear
R19778 T24788 T24794 nsubjpass extracts,separated
R19779 T24789 T24788 punct (,extracts
R19780 T24790 T24791 nummod 20,µg
R19781 T24791 T24788 appos µg,extracts
R19782 T24792 T24788 punct ),extracts
R19783 T24793 T24794 auxpass were,separated
R19784 T24794 T24794 ROOT separated,separated
R19785 T24795 T24794 prep by,separated
R19786 T24796 T24797 nummod 12,%
R19787 T24797 T24798 compound %,SDS
R19788 T24798 T24795 pobj SDS,by
R19789 T24799 T24798 conj PAGE,SDS
R19790 T24800 T24794 cc and,separated
R19791 T24801 T24794 conj analysed,separated
R19792 T24802 T24801 agent by,analysed
R19793 T24803 T24804 amod western,blotting
R19794 T24804 T24802 pobj blotting,by
R19795 T24805 T24801 advcl using,analysed
R19796 T24806 T24812 det the,H1
R19797 T24807 T24812 amod anti-p65,H1
R19798 T24808 T24812 punct ",",H1
R19799 T24809 T24812 amod anti-FLAG,H1
R19800 T24810 T24812 punct ",",H1
R19801 T24811 T24812 amod anti-Histone,H1
R19802 T24812 T24805 dobj H1,using
R19803 T24813 T24812 punct ",",H1
R19804 T24814 T24812 conj anti-IκB-α,H1
R19805 T24815 T24814 cc and,anti-IκB-α
R19806 T24816 T24814 conj anti-Hexokinase-II,anti-IκB-α
R19807 T24817 T24812 conj antibodies,H1
R19808 T24818 T24794 punct .,separated

GO-BP

Id Subject Object Predicate Lexical cue
T24996 201-214 http://purl.obolibrary.org/obo/GO_0006351 denotes Transcription

GO-MF

Id Subject Object Predicate Lexical cue
T24999 132-139 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T25000 132-152 http://purl.obolibrary.org/obo/GO_0051059 denotes binding to the NF-κB
T25001 437-447 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies

GO-CC

Id Subject Object Predicate Lexical cue
T24992 437-447 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T24994 437-447 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies

sentences

Id Subject Object Predicate Lexical cue
T24353 0-448 Sentence denotes Nuclear and cytosolic extracts were prepared for further analysis. (A) Nuclear extracts were analysed for the p65, p50 and FLAG-Tat binding to the NF-κB double-stranded oligonucleotide using the NF-κB Transcription Factor ELISA assay kit (Cayman). (B) Nuclear and cytosolic extracts (20 µg) were separated by 12% SDS–PAGE and analysed by western blotting using the anti-p65, anti-FLAG, anti-Histone H1, anti-IκB-α and anti-Hexokinase-II antibodies.
T133 0-448 Sentence denotes Nuclear and cytosolic extracts were prepared for further analysis. (A) Nuclear extracts were analysed for the p65, p50 and FLAG-Tat binding to the NF-κB double-stranded oligonucleotide using the NF-κB Transcription Factor ELISA assay kit (Cayman). (B) Nuclear and cytosolic extracts (20 µg) were separated by 12% SDS–PAGE and analysed by western blotting using the anti-p65, anti-FLAG, anti-Histone H1, anti-IκB-α and anti-Hexokinase-II antibodies.

events-check-again

Id Subject Object Predicate Lexical cue Speculation
T25064 110-113 Protein denotes p65
T25065 115-118 Protein denotes p50
T25066 123-131 Protein denotes FLAG-Tat
T25067 132-139 Binding denotes binding true
T25068 132-139 Binding denotes binding true
T25069 132-139 Binding denotes binding true
R20002 T25064 T25068 themeOf p65,binding
R20003 T25065 T25067 themeOf p50,binding
R20004 T25066 T25069 themeOf FLAG-Tat,binding

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T25079 110-113 Protein denotes p65
T25080 115-118 Protein denotes p50
T25081 123-127 Protein denotes FLAG
T25082 128-131 Protein denotes Tat
T25083 147-152 Protein denotes NF-κB
T25084 195-200 Protein denotes NF-κB
T25085 370-373 Protein denotes p65
T25086 375-384 Protein denotes anti-FLAG
T25087 386-413 Protein denotes anti-Histone H1, anti-IκB-α
T25088 418-447 Protein denotes anti-Hexokinase-II antibodies

test2

Id Subject Object Predicate Lexical cue
T24333 110-113 Protein denotes p65
T24334 115-118 Protein denotes p50
T24335 123-131 Protein denotes FLAG-Tat
T24336 132-139 Binding denotes binding
R19346 T24333 T24336 themeOf p65,binding
R19347 T24334 T24336 themeOf p50,binding
R19348 T24335 T24336 themeOf FLAG-Tat,binding

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue Speculation
T24343 110-113 Protein denotes p65
T24344 115-118 Protein denotes p50
T24345 123-131 Protein denotes FLAG-Tat
T24346 132-139 Binding denotes binding true
T24347 132-139 Binding denotes binding true
T24348 132-139 Binding denotes binding true
R19351 T24343 T24347 themeOf p65,binding
R19352 T24344 T24346 themeOf p50,binding
R19353 T24345 T24348 themeOf FLAG-Tat,binding

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T24649 110-113 Q04206 denotes p65
T24650 110-113 P21579 denotes p65
T24651 115-118 P19838 denotes p50
T24652 128-131 P04608 denotes Tat
T24653 128-131 P04605 denotes Tat
T24654 128-131 P04610 denotes Tat
T24655 128-131 Q8UMQ1 denotes Tat
T24656 370-373 P21579 denotes p65
T24657 370-373 Q04206 denotes p65