32PO4 Uptake and phosphorylation of total oocyte protein.
Untreated (control) and progesterone-stimulated (3.2 μM) denuded oocytes were incubated in 4.0 ml of Ringer's solution containing 80 μM [32PO4] at 20°C. Groups of six denuded oocytes were removed at the times indicated, rinsed with Ringer's solution, and homogenized in 2.0 ml 7% TCA at ice bath temperatures. The homogenate was allowed to stand for 5 min, then centrifuged and both washed precipitate and aliquots of the supernatant were counted. Uptake was corrected for estimated cell water content (50 ± 2%) and the specific activity of the medium expressed as μmoles/liter of cytoplasm.