Supporting Information
Figure S1  Expression of c-Rel does not enhance M-CSF-induced NF-κB transcriptional activity in macrophages. RAW 264.7 cells were transiently transfected with pTAL-SEAP, pNF-κB-SEAP or pNF-κB-SEAP + HA-cRel constructs. Cells were serum starved for 4 hours prior to the stimulation with mouse recombinant M-CSF (100 ng/ml) for 2 hours. Culture media was collected to measure SEAP production. Data are expressed as fold increase of SEAP activity over that in pTAL-SEAP transfected resting cells. The graph represents mean ± S.E.M for two independent experiments done in triplicates.
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