PMC:3245220 / 22158-22767
Annnotations
bionlp-st-ge-2016-uniprot
{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T25317","span":{"begin":58,"end":63},"obj":"P09603"},{"id":"T25318","span":{"begin":60,"end":63},"obj":"P04141"},{"id":"T25319","span":{"begin":259,"end":264},"obj":"P09603"},{"id":"T25320","span":{"begin":261,"end":264},"obj":"P04141"},{"id":"T25321","span":{"begin":397,"end":401},"obj":"P25963"},{"id":"T25322","span":{"begin":413,"end":418},"obj":"P04406"},{"id":"T25323","span":{"begin":442,"end":450},"obj":"Q04864"},{"id":"T25324","span":{"begin":468,"end":472},"obj":"P25963"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"10.1371/journal.pone.0028081.g006 Figure 6 Inhibition of M-CSF-induced PKC reduces NF-κB-regulated genes in both MDMs and RAW 264.7 cells.\nMDMs (A and C) and RAW 264.7 (B) cells were pretreated with Ro-31-8220 or solvent control DMSO for 30 minutes prior to M-CSF stimulation for the indicated times. Total RNA was isolated and converted to cDNA. Real-time RT PCR was performed using primers for IκBα, BCL-xl or GAPDH. Data are expressed as relative fold increase of IκBα or BCL-xl gene expression upon treatment over non-stimulated cells. Data represent the mean ± S.E.M for three independent experiments. "}
bionlp-st-ge-2016-reference
{"project":"bionlp-st-ge-2016-reference","denotations":[{"id":"T25325","span":{"begin":456,"end":464},"obj":"Positive_regulation"},{"id":"T25326","span":{"begin":488,"end":498},"obj":"Gene_expression"},{"id":"T25327","span":{"begin":456,"end":464},"obj":"Positive_regulation"},{"id":"T25328","span":{"begin":488,"end":498},"obj":"Gene_expression"},{"id":"T25329","span":{"begin":397,"end":401},"obj":"Protein"},{"id":"T25330","span":{"begin":403,"end":409},"obj":"Protein"},{"id":"T25331","span":{"begin":468,"end":472},"obj":"Protein"},{"id":"T25332","span":{"begin":476,"end":482},"obj":"Protein"},{"id":"T25333","span":{"begin":58,"end":63},"obj":"Protein"},{"id":"T25334","span":{"begin":259,"end":264},"obj":"Protein"},{"id":"T25335","span":{"begin":44,"end":54},"obj":"Negative_regulation"}],"relations":[{"id":"R19373","pred":"themeOf","subj":"T25326","obj":"T25325"},{"id":"R19374","pred":"themeOf","subj":"T25328","obj":"T25327"},{"id":"R19375","pred":"themeOf","subj":"T25331","obj":"T25326"},{"id":"R19376","pred":"themeOf","subj":"T25332","obj":"T25328"},{"id":"R19377","pred":"themeOf","subj":"T25333","obj":"T25335"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"10.1371/journal.pone.0028081.g006 Figure 6 Inhibition of M-CSF-induced PKC reduces NF-κB-regulated genes in both MDMs and RAW 264.7 cells.\nMDMs (A and C) and RAW 264.7 (B) cells were pretreated with Ro-31-8220 or solvent control DMSO for 30 minutes prior to M-CSF stimulation for the indicated times. Total RNA was isolated and converted to cDNA. Real-time RT PCR was performed using primers for IκBα, BCL-xl or GAPDH. Data are expressed as relative fold increase of IκBα or BCL-xl gene expression upon treatment over non-stimulated cells. Data represent the mean ± S.E.M for three independent experiments. "}
bionlp-st-ge-2016-reference-tees
{"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T25409","span":{"begin":58,"end":63},"obj":"Protein"},{"id":"T25410","span":{"begin":72,"end":75},"obj":"Protein"},{"id":"T25411","span":{"begin":84,"end":89},"obj":"Protein"},{"id":"T25412","span":{"begin":64,"end":71},"obj":"Positive_regulation"},{"id":"T25413","span":{"begin":44,"end":54},"obj":"Negative_regulation"},{"id":"T25414","span":{"begin":259,"end":264},"obj":"Protein"},{"id":"T25415","span":{"begin":397,"end":409},"obj":"Protein"},{"id":"T25416","span":{"begin":413,"end":418},"obj":"Protein"},{"id":"T25417","span":{"begin":468,"end":472},"obj":"Protein"},{"id":"T25418","span":{"begin":476,"end":479},"obj":"Protein"},{"id":"T25419","span":{"begin":488,"end":498},"obj":"Gene_expression"},{"id":"T25420","span":{"begin":488,"end":498},"obj":"Gene_expression"},{"id":"T25421","span":{"begin":456,"end":464},"obj":"Positive_regulation"},{"id":"T25422","span":{"begin":456,"end":464},"obj":"Positive_regulation"}],"relations":[{"id":"R19406","pred":"causeOf","subj":"T25409","obj":"T25412"},{"id":"R19407","pred":"themeOf","subj":"T25410","obj":"T25412"},{"id":"R19408","pred":"themeOf","subj":"T25412","obj":"T25413"},{"id":"R19409","pred":"themeOf","subj":"T25417","obj":"T25419"},{"id":"R19410","pred":"themeOf","subj":"T25418","obj":"T25420"},{"id":"R19411","pred":"themeOf","subj":"T25419","obj":"T25421"},{"id":"R19412","pred":"themeOf","subj":"T25420","obj":"T25422"}],"text":"10.1371/journal.pone.0028081.g006 Figure 6 Inhibition of M-CSF-induced PKC reduces NF-κB-regulated genes in both MDMs and RAW 264.7 cells.\nMDMs (A and C) and RAW 264.7 (B) cells were pretreated with Ro-31-8220 or solvent control DMSO for 30 minutes prior to M-CSF stimulation for the indicated times. Total RNA was isolated and converted to cDNA. Real-time RT PCR was performed using primers for IκBα, BCL-xl or GAPDH. Data are expressed as relative fold increase of IκBα or BCL-xl gene expression upon treatment over non-stimulated cells. Data represent the mean ± S.E.M for three independent experiments. "}
events-check-again
{"project":"events-check-again","denotations":[{"id":"T25398","span":{"begin":44,"end":54},"obj":"Negative_regulation"},{"id":"T25399","span":{"begin":58,"end":63},"obj":"Protein"},{"id":"T25400","span":{"begin":259,"end":264},"obj":"Protein"},{"id":"T25401","span":{"begin":397,"end":401},"obj":"Protein"},{"id":"T25402","span":{"begin":403,"end":409},"obj":"Protein"},{"id":"T25403","span":{"begin":456,"end":464},"obj":"Positive_regulation"},{"id":"T25404","span":{"begin":456,"end":464},"obj":"Positive_regulation"},{"id":"T25405","span":{"begin":468,"end":472},"obj":"Protein"},{"id":"T25406","span":{"begin":476,"end":482},"obj":"Protein"},{"id":"T25407","span":{"begin":488,"end":498},"obj":"Gene_expression"},{"id":"T25408","span":{"begin":488,"end":498},"obj":"Gene_expression"}],"relations":[{"id":"R19401","pred":"themeOf","subj":"T25399","obj":"T25398"},{"id":"R19402","pred":"themeOf","subj":"T25405","obj":"T25407"},{"id":"R19403","pred":"themeOf","subj":"T25406","obj":"T25408"},{"id":"R19404","pred":"themeOf","subj":"T25407","obj":"T25403"},{"id":"R19405","pred":"themeOf","subj":"T25408","obj":"T25404"}],"text":"10.1371/journal.pone.0028081.g006 Figure 6 Inhibition of M-CSF-induced PKC reduces NF-κB-regulated genes in both MDMs and RAW 264.7 cells.\nMDMs (A and C) and RAW 264.7 (B) cells were pretreated with Ro-31-8220 or solvent control DMSO for 30 minutes prior to M-CSF stimulation for the indicated times. Total RNA was isolated and converted to cDNA. Real-time RT PCR was performed using primers for IκBα, BCL-xl or GAPDH. Data are expressed as relative fold increase of IκBα or BCL-xl gene expression upon treatment over non-stimulated cells. Data represent the mean ± S.E.M for three independent experiments. "}
GO-CC
{"project":"GO-CC","denotations":[{"id":"T25344","span":{"begin":133,"end":138},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T25345","span":{"begin":173,"end":178},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T25346","span":{"begin":534,"end":539},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"10.1371/journal.pone.0028081.g006 Figure 6 Inhibition of M-CSF-induced PKC reduces NF-κB-regulated genes in both MDMs and RAW 264.7 cells.\nMDMs (A and C) and RAW 264.7 (B) cells were pretreated with Ro-31-8220 or solvent control DMSO for 30 minutes prior to M-CSF stimulation for the indicated times. Total RNA was isolated and converted to cDNA. Real-time RT PCR was performed using primers for IκBα, BCL-xl or GAPDH. Data are expressed as relative fold increase of IκBα or BCL-xl gene expression upon treatment over non-stimulated cells. Data represent the mean ± S.E.M for three independent experiments. "}
GO-MF
{"project":"GO-MF","denotations":[{"id":"T25342","span":{"begin":72,"end":75},"obj":"http://purl.obolibrary.org/obo/GO_0004697"},{"id":"T25343","span":{"begin":358,"end":360},"obj":"http://purl.obolibrary.org/obo/GO_0003964"}],"text":"10.1371/journal.pone.0028081.g006 Figure 6 Inhibition of M-CSF-induced PKC reduces NF-κB-regulated genes in both MDMs and RAW 264.7 cells.\nMDMs (A and C) and RAW 264.7 (B) cells were pretreated with Ro-31-8220 or solvent control DMSO for 30 minutes prior to M-CSF stimulation for the indicated times. Total RNA was isolated and converted to cDNA. Real-time RT PCR was performed using primers for IκBα, BCL-xl or GAPDH. Data are expressed as relative fold increase of IκBα or BCL-xl gene expression upon treatment over non-stimulated cells. Data represent the mean ± S.E.M for three independent experiments. "}
GO-BP
{"project":"GO-BP","denotations":[{"id":"T25423","span":{"begin":358,"end":360},"obj":"http://purl.obolibrary.org/obo/GO_0003964"},{"id":"T25424","span":{"begin":483,"end":498},"obj":"http://purl.obolibrary.org/obo/GO_0010467"}],"text":"10.1371/journal.pone.0028081.g006 Figure 6 Inhibition of M-CSF-induced PKC reduces NF-κB-regulated genes in both MDMs and RAW 264.7 cells.\nMDMs (A and C) and RAW 264.7 (B) cells were pretreated with Ro-31-8220 or solvent control DMSO for 30 minutes prior to M-CSF stimulation for the indicated times. Total RNA was isolated and converted to cDNA. Real-time RT PCR was performed using primers for IκBα, BCL-xl or GAPDH. Data are expressed as relative fold increase of IκBα or BCL-xl gene expression upon treatment over non-stimulated cells. Data represent the mean ± S.E.M for three independent experiments. "}
pmc-enju-pas
{"project":"pmc-enju-pas","denotations":[{"id":"T25222","span":{"begin":44,"end":54},"obj":"NN"},{"id":"T25223","span":{"begin":55,"end":57},"obj":"IN"},{"id":"T25224","span":{"begin":58,"end":71},"obj":"JJ"},{"id":"T25225","span":{"begin":72,"end":75},"obj":"NN"},{"id":"T25226","span":{"begin":76,"end":83},"obj":"VB"},{"id":"T25227","span":{"begin":84,"end":99},"obj":"JJ"},{"id":"T25228","span":{"begin":100,"end":105},"obj":"NN"},{"id":"T25229","span":{"begin":106,"end":108},"obj":"IN"},{"id":"T25230","span":{"begin":109,"end":113},"obj":"CC"},{"id":"T25231","span":{"begin":114,"end":118},"obj":"NN"},{"id":"T25232","span":{"begin":119,"end":122},"obj":"CC"},{"id":"T25233","span":{"begin":123,"end":126},"obj":"NN"},{"id":"T25234","span":{"begin":127,"end":132},"obj":"CD"},{"id":"T25235","span":{"begin":133,"end":138},"obj":"NN"},{"id":"T25236","span":{"begin":140,"end":144},"obj":"NN"},{"id":"T25237","span":{"begin":145,"end":146},"obj":"-LRB-"},{"id":"T25238","span":{"begin":146,"end":147},"obj":"NN"},{"id":"T25239","span":{"begin":148,"end":151},"obj":"CC"},{"id":"T25240","span":{"begin":152,"end":153},"obj":"NN"},{"id":"T25241","span":{"begin":153,"end":154},"obj":"-RRB-"},{"id":"T25242","span":{"begin":155,"end":158},"obj":"CC"},{"id":"T25243","span":{"begin":159,"end":162},"obj":"NN"},{"id":"T25244","span":{"begin":163,"end":168},"obj":"CD"},{"id":"T25245","span":{"begin":169,"end":170},"obj":"-LRB-"},{"id":"T25246","span":{"begin":170,"end":171},"obj":"NN"},{"id":"T25247","span":{"begin":171,"end":172},"obj":"-RRB-"},{"id":"T25248","span":{"begin":173,"end":178},"obj":"NN"},{"id":"T25249","span":{"begin":179,"end":183},"obj":"VB"},{"id":"T25250","span":{"begin":184,"end":194},"obj":"VB"},{"id":"T25251","span":{"begin":195,"end":199},"obj":"IN"},{"id":"T25252","span":{"begin":200,"end":210},"obj":"NN"},{"id":"T25253","span":{"begin":211,"end":213},"obj":"CC"},{"id":"T25254","span":{"begin":214,"end":221},"obj":"NN"},{"id":"T25255","span":{"begin":222,"end":229},"obj":"NN"},{"id":"T25256","span":{"begin":230,"end":234},"obj":"NN"},{"id":"T25257","span":{"begin":235,"end":238},"obj":"IN"},{"id":"T25258","span":{"begin":239,"end":241},"obj":"CD"},{"id":"T25259","span":{"begin":242,"end":249},"obj":"NN"},{"id":"T25260","span":{"begin":250,"end":255},"obj":"RB"},{"id":"T25261","span":{"begin":256,"end":258},"obj":"TO"},{"id":"T25262","span":{"begin":259,"end":264},"obj":"NN"},{"id":"T25263","span":{"begin":265,"end":276},"obj":"NN"},{"id":"T25264","span":{"begin":277,"end":280},"obj":"IN"},{"id":"T25265","span":{"begin":281,"end":284},"obj":"DT"},{"id":"T25266","span":{"begin":285,"end":294},"obj":"JJ"},{"id":"T25267","span":{"begin":295,"end":300},"obj":"NN"},{"id":"T25268","span":{"begin":302,"end":307},"obj":"JJ"},{"id":"T25269","span":{"begin":308,"end":311},"obj":"NN"},{"id":"T25270","span":{"begin":312,"end":315},"obj":"VB"},{"id":"T25271","span":{"begin":316,"end":324},"obj":"VB"},{"id":"T25272","span":{"begin":325,"end":328},"obj":"CC"},{"id":"T25273","span":{"begin":329,"end":338},"obj":"VB"},{"id":"T25274","span":{"begin":339,"end":341},"obj":"TO"},{"id":"T25275","span":{"begin":342,"end":346},"obj":"NN"},{"id":"T25276","span":{"begin":348,"end":357},"obj":"JJ"},{"id":"T25277","span":{"begin":358,"end":360},"obj":"NN"},{"id":"T25278","span":{"begin":361,"end":364},"obj":"NN"},{"id":"T25279","span":{"begin":365,"end":368},"obj":"VB"},{"id":"T25280","span":{"begin":369,"end":378},"obj":"VB"},{"id":"T25281","span":{"begin":379,"end":384},"obj":"VB"},{"id":"T25282","span":{"begin":385,"end":392},"obj":"NN"},{"id":"T25283","span":{"begin":393,"end":396},"obj":"IN"},{"id":"T25284","span":{"begin":397,"end":401},"obj":"NNP"},{"id":"T25285","span":{"begin":401,"end":402},"obj":"-COMMA-"},{"id":"T25286","span":{"begin":403,"end":409},"obj":"NN"},{"id":"T25287","span":{"begin":410,"end":412},"obj":"CC"},{"id":"T25288","span":{"begin":413,"end":418},"obj":"NN"},{"id":"T25289","span":{"begin":420,"end":424},"obj":"NN"},{"id":"T25290","span":{"begin":425,"end":428},"obj":"VB"},{"id":"T25291","span":{"begin":429,"end":438},"obj":"VB"},{"id":"T25292","span":{"begin":439,"end":441},"obj":"IN"},{"id":"T25293","span":{"begin":442,"end":450},"obj":"JJ"},{"id":"T25294","span":{"begin":451,"end":455},"obj":"JJ"},{"id":"T25295","span":{"begin":456,"end":464},"obj":"NN"},{"id":"T25296","span":{"begin":465,"end":467},"obj":"IN"},{"id":"T25297","span":{"begin":468,"end":472},"obj":"NN"},{"id":"T25298","span":{"begin":473,"end":475},"obj":"CC"},{"id":"T25299","span":{"begin":476,"end":482},"obj":"JJ"},{"id":"T25300","span":{"begin":483,"end":487},"obj":"NN"},{"id":"T25301","span":{"begin":488,"end":498},"obj":"NN"},{"id":"T25302","span":{"begin":499,"end":503},"obj":"IN"},{"id":"T25303","span":{"begin":504,"end":513},"obj":"NN"},{"id":"T25304","span":{"begin":514,"end":518},"obj":"IN"},{"id":"T25305","span":{"begin":519,"end":533},"obj":"JJ"},{"id":"T25306","span":{"begin":534,"end":539},"obj":"NN"},{"id":"T25307","span":{"begin":541,"end":545},"obj":"NN"},{"id":"T25308","span":{"begin":546,"end":555},"obj":"VB"},{"id":"T25309","span":{"begin":556,"end":559},"obj":"DT"},{"id":"T25310","span":{"begin":560,"end":564},"obj":"JJ"},{"id":"T25311","span":{"begin":565,"end":566},"obj":"JJ"},{"id":"T25312","span":{"begin":567,"end":572},"obj":"NN"},{"id":"T25313","span":{"begin":573,"end":576},"obj":"IN"},{"id":"T25314","span":{"begin":577,"end":582},"obj":"CD"},{"id":"T25315","span":{"begin":583,"end":594},"obj":"JJ"},{"id":"T25316","span":{"begin":595,"end":606},"obj":"NN"}],"relations":[{"id":"R19278","pred":"arg1Of","subj":"T25222","obj":"T25223"},{"id":"R19279","pred":"arg1Of","subj":"T25222","obj":"T25226"},{"id":"R19280","pred":"arg2Of","subj":"T25225","obj":"T25223"},{"id":"R19281","pred":"arg1Of","subj":"T25225","obj":"T25224"},{"id":"R19282","pred":"arg1Of","subj":"T25226","obj":"T25229"},{"id":"R19283","pred":"arg2Of","subj":"T25228","obj":"T25226"},{"id":"R19284","pred":"arg1Of","subj":"T25228","obj":"T25227"},{"id":"R19285","pred":"arg1Of","subj":"T25231","obj":"T25232"},{"id":"R19286","pred":"arg2Of","subj":"T25232","obj":"T25229"},{"id":"R19287","pred":"arg1Of","subj":"T25232","obj":"T25230"},{"id":"R19288","pred":"arg2Of","subj":"T25235","obj":"T25232"},{"id":"R19289","pred":"arg1Of","subj":"T25235","obj":"T25233"},{"id":"R19290","pred":"arg1Of","subj":"T25235","obj":"T25234"},{"id":"R19291","pred":"arg1Of","subj":"T25236","obj":"T25237"},{"id":"R19292","pred":"arg1Of","subj":"T25236","obj":"T25242"},{"id":"R19293","pred":"arg1Of","subj":"T25238","obj":"T25239"},{"id":"R19294","pred":"arg2Of","subj":"T25239","obj":"T25237"},{"id":"R19295","pred":"arg2Of","subj":"T25240","obj":"T25239"},{"id":"R19296","pred":"arg3Of","subj":"T25241","obj":"T25237"},{"id":"R19297","pred":"arg1Of","subj":"T25242","obj":"T25249"},{"id":"R19298","pred":"arg2Of","subj":"T25242","obj":"T25250"},{"id":"R19299","pred":"arg1Of","subj":"T25243","obj":"T25244"},{"id":"R19300","pred":"arg1Of","subj":"T25243","obj":"T25245"},{"id":"R19301","pred":"arg2Of","subj":"T25246","obj":"T25245"},{"id":"R19302","pred":"arg3Of","subj":"T25247","obj":"T25245"},{"id":"R19303","pred":"arg2Of","subj":"T25248","obj":"T25242"},{"id":"R19304","pred":"arg1Of","subj":"T25248","obj":"T25243"},{"id":"R19305","pred":"arg2Of","subj":"T25250","obj":"T25249"},{"id":"R19306","pred":"arg1Of","subj":"T25250","obj":"T25251"},{"id":"R19307","pred":"arg1Of","subj":"T25250","obj":"T25257"},{"id":"R19308","pred":"arg1Of","subj":"T25252","obj":"T25253"},{"id":"R19309","pred":"arg2Of","subj":"T25253","obj":"T25251"},{"id":"R19310","pred":"arg2Of","subj":"T25256","obj":"T25253"},{"id":"R19311","pred":"arg1Of","subj":"T25256","obj":"T25254"},{"id":"R19312","pred":"arg1Of","subj":"T25256","obj":"T25255"},{"id":"R19313","pred":"arg2Of","subj":"T25259","obj":"T25257"},{"id":"R19314","pred":"arg1Of","subj":"T25259","obj":"T25258"},{"id":"R19315","pred":"arg1Of","subj":"T25259","obj":"T25261"},{"id":"R19316","pred":"arg1Of","subj":"T25261","obj":"T25260"},{"id":"R19317","pred":"arg2Of","subj":"T25263","obj":"T25261"},{"id":"R19318","pred":"arg1Of","subj":"T25263","obj":"T25262"},{"id":"R19319","pred":"arg1Of","subj":"T25263","obj":"T25264"},{"id":"R19320","pred":"arg2Of","subj":"T25267","obj":"T25264"},{"id":"R19321","pred":"arg1Of","subj":"T25267","obj":"T25265"},{"id":"R19322","pred":"arg1Of","subj":"T25267","obj":"T25266"},{"id":"R19323","pred":"arg1Of","subj":"T25269","obj":"T25268"},{"id":"R19324","pred":"arg1Of","subj":"T25269","obj":"T25270"},{"id":"R19325","pred":"arg2Of","subj":"T25269","obj":"T25271"},{"id":"R19326","pred":"arg2Of","subj":"T25269","obj":"T25273"},{"id":"R19327","pred":"arg1Of","subj":"T25271","obj":"T25272"},{"id":"R19328","pred":"arg2Of","subj":"T25272","obj":"T25270"},{"id":"R19329","pred":"arg2Of","subj":"T25273","obj":"T25272"},{"id":"R19330","pred":"arg1Of","subj":"T25273","obj":"T25274"},{"id":"R19331","pred":"arg2Of","subj":"T25275","obj":"T25274"},{"id":"R19332","pred":"arg1Of","subj":"T25278","obj":"T25276"},{"id":"R19333","pred":"arg1Of","subj":"T25278","obj":"T25277"},{"id":"R19334","pred":"arg1Of","subj":"T25278","obj":"T25279"},{"id":"R19335","pred":"arg2Of","subj":"T25278","obj":"T25280"},{"id":"R19336","pred":"arg2Of","subj":"T25280","obj":"T25279"},{"id":"R19337","pred":"arg3Of","subj":"T25281","obj":"T25280"},{"id":"R19338","pred":"arg2Of","subj":"T25282","obj":"T25281"},{"id":"R19339","pred":"arg1Of","subj":"T25282","obj":"T25283"},{"id":"R19340","pred":"arg1Of","subj":"T25284","obj":"T25285"},{"id":"R19341","pred":"arg1Of","subj":"T25285","obj":"T25287"},{"id":"R19342","pred":"arg2Of","subj":"T25286","obj":"T25285"},{"id":"R19343","pred":"arg2Of","subj":"T25287","obj":"T25283"},{"id":"R19344","pred":"arg2Of","subj":"T25288","obj":"T25287"},{"id":"R19345","pred":"arg1Of","subj":"T25289","obj":"T25290"},{"id":"R19346","pred":"arg2Of","subj":"T25289","obj":"T25291"},{"id":"R19347","pred":"arg2Of","subj":"T25291","obj":"T25290"},{"id":"R19348","pred":"arg1Of","subj":"T25291","obj":"T25292"},{"id":"R19349","pred":"arg1Of","subj":"T25294","obj":"T25293"},{"id":"R19350","pred":"arg2Of","subj":"T25295","obj":"T25292"},{"id":"R19351","pred":"arg1Of","subj":"T25295","obj":"T25294"},{"id":"R19352","pred":"arg1Of","subj":"T25295","obj":"T25296"},{"id":"R19353","pred":"arg1Of","subj":"T25297","obj":"T25298"},{"id":"R19354","pred":"arg2Of","subj":"T25299","obj":"T25298"},{"id":"R19355","pred":"arg2Of","subj":"T25301","obj":"T25296"},{"id":"R19356","pred":"arg1Of","subj":"T25301","obj":"T25297"},{"id":"R19357","pred":"arg1Of","subj":"T25301","obj":"T25299"},{"id":"R19358","pred":"arg1Of","subj":"T25301","obj":"T25300"},{"id":"R19359","pred":"arg1Of","subj":"T25301","obj":"T25302"},{"id":"R19360","pred":"arg1Of","subj":"T25301","obj":"T25304"},{"id":"R19361","pred":"arg2Of","subj":"T25303","obj":"T25302"},{"id":"R19362","pred":"arg2Of","subj":"T25306","obj":"T25304"},{"id":"R19363","pred":"arg1Of","subj":"T25306","obj":"T25305"},{"id":"R19364","pred":"arg1Of","subj":"T25307","obj":"T25308"},{"id":"R19365","pred":"arg2Of","subj":"T25312","obj":"T25308"},{"id":"R19366","pred":"arg1Of","subj":"T25312","obj":"T25309"},{"id":"R19367","pred":"arg1Of","subj":"T25312","obj":"T25310"},{"id":"R19368","pred":"arg1Of","subj":"T25312","obj":"T25311"},{"id":"R19369","pred":"arg1Of","subj":"T25312","obj":"T25313"},{"id":"R19370","pred":"arg2Of","subj":"T25316","obj":"T25313"},{"id":"R19371","pred":"arg1Of","subj":"T25316","obj":"T25314"},{"id":"R19372","pred":"arg1Of","subj":"T25316","obj":"T25315"}],"namespaces":[{"prefix":"_base","uri":"http://kmcs.nii.ac.jp/enju/"}],"text":"10.1371/journal.pone.0028081.g006 Figure 6 Inhibition of M-CSF-induced PKC reduces NF-κB-regulated genes in both MDMs and RAW 264.7 cells.\nMDMs (A and C) and RAW 264.7 (B) cells were pretreated with Ro-31-8220 or solvent control DMSO for 30 minutes prior to M-CSF stimulation for the indicated times. Total RNA was isolated and converted to cDNA. Real-time RT PCR was performed using primers for IκBα, BCL-xl or GAPDH. Data are expressed as relative fold increase of IκBα or BCL-xl gene expression upon treatment over non-stimulated cells. Data represent the mean ± S.E.M for three independent experiments. "}
sentences
{"project":"sentences","denotations":[{"id":"T25115","span":{"begin":44,"end":139},"obj":"Sentence"},{"id":"T25116","span":{"begin":140,"end":301},"obj":"Sentence"},{"id":"T25117","span":{"begin":302,"end":347},"obj":"Sentence"},{"id":"T25118","span":{"begin":348,"end":419},"obj":"Sentence"},{"id":"T25119","span":{"begin":420,"end":540},"obj":"Sentence"},{"id":"T25120","span":{"begin":541,"end":607},"obj":"Sentence"},{"id":"T159","span":{"begin":0,"end":139},"obj":"Sentence"},{"id":"T160","span":{"begin":140,"end":301},"obj":"Sentence"},{"id":"T161","span":{"begin":302,"end":347},"obj":"Sentence"},{"id":"T162","span":{"begin":348,"end":419},"obj":"Sentence"},{"id":"T163","span":{"begin":420,"end":540},"obj":"Sentence"},{"id":"T164","span":{"begin":541,"end":607},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"10.1371/journal.pone.0028081.g006 Figure 6 Inhibition of M-CSF-induced PKC reduces NF-κB-regulated genes in both MDMs and RAW 264.7 cells.\nMDMs (A and C) and RAW 264.7 (B) cells were pretreated with Ro-31-8220 or solvent control DMSO for 30 minutes prior to M-CSF stimulation for the indicated times. Total RNA was isolated and converted to cDNA. Real-time RT PCR was performed using primers for IκBα, BCL-xl or GAPDH. Data are expressed as relative fold increase of IκBα or BCL-xl gene expression upon treatment over non-stimulated cells. Data represent the mean ± S.E.M for three independent experiments. "}
bionlp-st-ge-2016-spacy-parsed
{"project":"bionlp-st-ge-2016-spacy-parsed","denotations":[{"id":"T25121","span":{"begin":44,"end":54},"obj":"NNP"},{"id":"T25122","span":{"begin":55,"end":57},"obj":"IN"},{"id":"T25123","span":{"begin":58,"end":71},"obj":"NNP"},{"id":"T25124","span":{"begin":72,"end":75},"obj":"NNP"},{"id":"T25125","span":{"begin":76,"end":83},"obj":"VBZ"},{"id":"T25126","span":{"begin":84,"end":99},"obj":"JJ"},{"id":"T25127","span":{"begin":100,"end":105},"obj":"NNS"},{"id":"T25128","span":{"begin":106,"end":108},"obj":"IN"},{"id":"T25129","span":{"begin":109,"end":113},"obj":"DT"},{"id":"T25130","span":{"begin":114,"end":118},"obj":"NNS"},{"id":"T25131","span":{"begin":119,"end":122},"obj":"CC"},{"id":"T25132","span":{"begin":123,"end":126},"obj":"NNP"},{"id":"T25133","span":{"begin":127,"end":132},"obj":"CD"},{"id":"T25134","span":{"begin":133,"end":138},"obj":"NNS"},{"id":"T25135","span":{"begin":138,"end":139},"obj":"."},{"id":"T25136","span":{"begin":140,"end":144},"obj":"NNS"},{"id":"T25137","span":{"begin":145,"end":146},"obj":"-LRB-"},{"id":"T25138","span":{"begin":146,"end":147},"obj":"DT"},{"id":"T25139","span":{"begin":148,"end":151},"obj":"CC"},{"id":"T25140","span":{"begin":152,"end":153},"obj":"NNP"},{"id":"T25141","span":{"begin":153,"end":154},"obj":"-RRB-"},{"id":"T25142","span":{"begin":155,"end":158},"obj":"CC"},{"id":"T25143","span":{"begin":159,"end":162},"obj":"NNP"},{"id":"T25144","span":{"begin":163,"end":168},"obj":"CD"},{"id":"T25145","span":{"begin":169,"end":170},"obj":"-LRB-"},{"id":"T25146","span":{"begin":170,"end":171},"obj":"NN"},{"id":"T25147","span":{"begin":171,"end":172},"obj":"-RRB-"},{"id":"T25148","span":{"begin":173,"end":178},"obj":"NNS"},{"id":"T25149","span":{"begin":179,"end":183},"obj":"VBD"},{"id":"T25150","span":{"begin":184,"end":194},"obj":"VBN"},{"id":"T25151","span":{"begin":195,"end":199},"obj":"IN"},{"id":"T25152","span":{"begin":200,"end":210},"obj":"NN"},{"id":"T25153","span":{"begin":211,"end":213},"obj":"CC"},{"id":"T25154","span":{"begin":214,"end":221},"obj":"JJ"},{"id":"T25155","span":{"begin":222,"end":229},"obj":"NN"},{"id":"T25156","span":{"begin":230,"end":234},"obj":"NNP"},{"id":"T25157","span":{"begin":235,"end":238},"obj":"IN"},{"id":"T25158","span":{"begin":239,"end":241},"obj":"CD"},{"id":"T25159","span":{"begin":242,"end":249},"obj":"NNS"},{"id":"T25160","span":{"begin":250,"end":255},"obj":"RB"},{"id":"T25161","span":{"begin":256,"end":258},"obj":"TO"},{"id":"T25162","span":{"begin":259,"end":264},"obj":"NNP"},{"id":"T25163","span":{"begin":265,"end":276},"obj":"NN"},{"id":"T25164","span":{"begin":277,"end":280},"obj":"IN"},{"id":"T25165","span":{"begin":281,"end":284},"obj":"DT"},{"id":"T25166","span":{"begin":285,"end":294},"obj":"JJ"},{"id":"T25167","span":{"begin":295,"end":300},"obj":"NNS"},{"id":"T25168","span":{"begin":300,"end":301},"obj":"."},{"id":"T25169","span":{"begin":302,"end":307},"obj":"JJ"},{"id":"T25170","span":{"begin":308,"end":311},"obj":"NNP"},{"id":"T25171","span":{"begin":312,"end":315},"obj":"VBD"},{"id":"T25172","span":{"begin":316,"end":324},"obj":"VBN"},{"id":"T25173","span":{"begin":325,"end":328},"obj":"CC"},{"id":"T25174","span":{"begin":329,"end":338},"obj":"VBN"},{"id":"T25175","span":{"begin":339,"end":341},"obj":"TO"},{"id":"T25176","span":{"begin":342,"end":346},"obj":"NNP"},{"id":"T25177","span":{"begin":346,"end":347},"obj":"."},{"id":"T25178","span":{"begin":348,"end":357},"obj":"JJ"},{"id":"T25179","span":{"begin":358,"end":360},"obj":"NNP"},{"id":"T25180","span":{"begin":361,"end":364},"obj":"NNP"},{"id":"T25181","span":{"begin":365,"end":368},"obj":"VBD"},{"id":"T25182","span":{"begin":369,"end":378},"obj":"VBN"},{"id":"T25183","span":{"begin":379,"end":384},"obj":"VBG"},{"id":"T25184","span":{"begin":385,"end":392},"obj":"NNS"},{"id":"T25185","span":{"begin":393,"end":396},"obj":"IN"},{"id":"T25186","span":{"begin":397,"end":401},"obj":"NNP"},{"id":"T25187","span":{"begin":401,"end":402},"obj":","},{"id":"T25188","span":{"begin":403,"end":409},"obj":"NNP"},{"id":"T25189","span":{"begin":410,"end":412},"obj":"CC"},{"id":"T25190","span":{"begin":413,"end":418},"obj":"NNP"},{"id":"T25191","span":{"begin":418,"end":419},"obj":"."},{"id":"T25192","span":{"begin":420,"end":424},"obj":"NNS"},{"id":"T25193","span":{"begin":425,"end":428},"obj":"VBP"},{"id":"T25194","span":{"begin":429,"end":438},"obj":"VBN"},{"id":"T25195","span":{"begin":439,"end":441},"obj":"IN"},{"id":"T25196","span":{"begin":442,"end":450},"obj":"JJ"},{"id":"T25197","span":{"begin":451,"end":455},"obj":"VBP"},{"id":"T25198","span":{"begin":456,"end":464},"obj":"NN"},{"id":"T25199","span":{"begin":465,"end":467},"obj":"IN"},{"id":"T25200","span":{"begin":468,"end":472},"obj":"NNP"},{"id":"T25201","span":{"begin":473,"end":475},"obj":"CC"},{"id":"T25202","span":{"begin":476,"end":482},"obj":"NNP"},{"id":"T25203","span":{"begin":483,"end":487},"obj":"NN"},{"id":"T25204","span":{"begin":488,"end":498},"obj":"NN"},{"id":"T25205","span":{"begin":499,"end":503},"obj":"IN"},{"id":"T25206","span":{"begin":504,"end":513},"obj":"NN"},{"id":"T25207","span":{"begin":514,"end":518},"obj":"IN"},{"id":"T25208","span":{"begin":519,"end":533},"obj":"JJ"},{"id":"T25209","span":{"begin":534,"end":539},"obj":"NNS"},{"id":"T25210","span":{"begin":539,"end":540},"obj":"."},{"id":"T25211","span":{"begin":541,"end":545},"obj":"NNP"},{"id":"T25212","span":{"begin":546,"end":555},"obj":"VBP"},{"id":"T25213","span":{"begin":556,"end":559},"obj":"DT"},{"id":"T25214","span":{"begin":560,"end":564},"obj":"JJ"},{"id":"T25215","span":{"begin":565,"end":566},"obj":"NN"},{"id":"T25216","span":{"begin":567,"end":572},"obj":"NNP"},{"id":"T25217","span":{"begin":573,"end":576},"obj":"IN"},{"id":"T25218","span":{"begin":577,"end":582},"obj":"CD"},{"id":"T25219","span":{"begin":583,"end":594},"obj":"JJ"},{"id":"T25220","span":{"begin":595,"end":606},"obj":"NNS"},{"id":"T25221","span":{"begin":606,"end":607},"obj":"."}],"relations":[{"id":"R19177","pred":"nsubj","subj":"T25121","obj":"T25125"},{"id":"R19178","pred":"prep","subj":"T25122","obj":"T25121"},{"id":"R19179","pred":"compound","subj":"T25123","obj":"T25124"},{"id":"R19180","pred":"pobj","subj":"T25124","obj":"T25122"},{"id":"R19181","pred":"ROOT","subj":"T25125","obj":"T25125"},{"id":"R19182","pred":"amod","subj":"T25126","obj":"T25127"},{"id":"R19183","pred":"dobj","subj":"T25127","obj":"T25125"},{"id":"R19184","pred":"prep","subj":"T25128","obj":"T25127"},{"id":"R19185","pred":"preconj","subj":"T25129","obj":"T25130"},{"id":"R19186","pred":"pobj","subj":"T25130","obj":"T25128"},{"id":"R19187","pred":"cc","subj":"T25131","obj":"T25130"},{"id":"R19188","pred":"conj","subj":"T25132","obj":"T25130"},{"id":"R19189","pred":"nummod","subj":"T25133","obj":"T25134"},{"id":"R19190","pred":"conj","subj":"T25134","obj":"T25130"},{"id":"R19191","pred":"punct","subj":"T25135","obj":"T25125"},{"id":"R19192","pred":"ROOT","subj":"T25136","obj":"T25136"},{"id":"R19193","pred":"punct","subj":"T25137","obj":"T25138"},{"id":"R19194","pred":"appos","subj":"T25138","obj":"T25136"},{"id":"R19195","pred":"cc","subj":"T25139","obj":"T25138"},{"id":"R19196","pred":"nmod","subj":"T25140","obj":"T25148"},{"id":"R19197","pred":"punct","subj":"T25141","obj":"T25140"},{"id":"R19198","pred":"cc","subj":"T25142","obj":"T25140"},{"id":"R19199","pred":"conj","subj":"T25143","obj":"T25140"},{"id":"R19200","pred":"nummod","subj":"T25144","obj":"T25143"},{"id":"R19201","pred":"punct","subj":"T25145","obj":"T25146"},{"id":"R19202","pred":"appos","subj":"T25146","obj":"T25143"},{"id":"R19203","pred":"punct","subj":"T25147","obj":"T25143"},{"id":"R19204","pred":"nsubjpass","subj":"T25148","obj":"T25150"},{"id":"R19205","pred":"auxpass","subj":"T25149","obj":"T25150"},{"id":"R19206","pred":"conj","subj":"T25150","obj":"T25138"},{"id":"R19207","pred":"prep","subj":"T25151","obj":"T25150"},{"id":"R19208","pred":"pobj","subj":"T25152","obj":"T25151"},{"id":"R19209","pred":"cc","subj":"T25153","obj":"T25152"},{"id":"R19210","pred":"amod","subj":"T25154","obj":"T25155"},{"id":"R19211","pred":"compound","subj":"T25155","obj":"T25156"},{"id":"R19212","pred":"conj","subj":"T25156","obj":"T25152"},{"id":"R19213","pred":"prep","subj":"T25157","obj":"T25150"},{"id":"R19214","pred":"nummod","subj":"T25158","obj":"T25159"},{"id":"R19215","pred":"pobj","subj":"T25159","obj":"T25157"},{"id":"R19216","pred":"advmod","subj":"T25160","obj":"T25150"},{"id":"R19217","pred":"prep","subj":"T25161","obj":"T25160"},{"id":"R19218","pred":"compound","subj":"T25162","obj":"T25163"},{"id":"R19219","pred":"pobj","subj":"T25163","obj":"T25161"},{"id":"R19220","pred":"prep","subj":"T25164","obj":"T25160"},{"id":"R19221","pred":"det","subj":"T25165","obj":"T25167"},{"id":"R19222","pred":"amod","subj":"T25166","obj":"T25167"},{"id":"R19223","pred":"pobj","subj":"T25167","obj":"T25164"},{"id":"R19224","pred":"punct","subj":"T25168","obj":"T25150"},{"id":"R19225","pred":"amod","subj":"T25169","obj":"T25170"},{"id":"R19226","pred":"nsubj","subj":"T25170","obj":"T25171"},{"id":"R19227","pred":"ROOT","subj":"T25171","obj":"T25171"},{"id":"R19228","pred":"acomp","subj":"T25172","obj":"T25171"},{"id":"R19229","pred":"cc","subj":"T25173","obj":"T25172"},{"id":"R19230","pred":"conj","subj":"T25174","obj":"T25172"},{"id":"R19231","pred":"prep","subj":"T25175","obj":"T25174"},{"id":"R19232","pred":"pobj","subj":"T25176","obj":"T25175"},{"id":"R19233","pred":"punct","subj":"T25177","obj":"T25171"},{"id":"R19234","pred":"amod","subj":"T25178","obj":"T25180"},{"id":"R19235","pred":"compound","subj":"T25179","obj":"T25180"},{"id":"R19236","pred":"nsubjpass","subj":"T25180","obj":"T25182"},{"id":"R19237","pred":"auxpass","subj":"T25181","obj":"T25182"},{"id":"R19238","pred":"ROOT","subj":"T25182","obj":"T25182"},{"id":"R19239","pred":"advcl","subj":"T25183","obj":"T25182"},{"id":"R19240","pred":"dobj","subj":"T25184","obj":"T25183"},{"id":"R19241","pred":"prep","subj":"T25185","obj":"T25183"},{"id":"R19242","pred":"pobj","subj":"T25186","obj":"T25185"},{"id":"R19243","pred":"punct","subj":"T25187","obj":"T25186"},{"id":"R19244","pred":"conj","subj":"T25188","obj":"T25186"},{"id":"R19245","pred":"cc","subj":"T25189","obj":"T25188"},{"id":"R19246","pred":"conj","subj":"T25190","obj":"T25188"},{"id":"R19247","pred":"punct","subj":"T25191","obj":"T25182"},{"id":"R19248","pred":"nsubjpass","subj":"T25192","obj":"T25194"},{"id":"R19249","pred":"auxpass","subj":"T25193","obj":"T25194"},{"id":"R19250","pred":"ROOT","subj":"T25194","obj":"T25194"},{"id":"R19251","pred":"prep","subj":"T25195","obj":"T25194"},{"id":"R19252","pred":"amod","subj":"T25196","obj":"T25197"},{"id":"R19253","pred":"compound","subj":"T25197","obj":"T25198"},{"id":"R19254","pred":"pobj","subj":"T25198","obj":"T25195"},{"id":"R19255","pred":"prep","subj":"T25199","obj":"T25198"},{"id":"R19256","pred":"pobj","subj":"T25200","obj":"T25199"},{"id":"R19257","pred":"cc","subj":"T25201","obj":"T25200"},{"id":"R19258","pred":"conj","subj":"T25202","obj":"T25200"},{"id":"R19259","pred":"compound","subj":"T25203","obj":"T25204"},{"id":"R19260","pred":"conj","subj":"T25204","obj":"T25198"},{"id":"R19261","pred":"prep","subj":"T25205","obj":"T25204"},{"id":"R19262","pred":"pobj","subj":"T25206","obj":"T25205"},{"id":"R19263","pred":"prep","subj":"T25207","obj":"T25204"},{"id":"R19264","pred":"amod","subj":"T25208","obj":"T25209"},{"id":"R19265","pred":"pobj","subj":"T25209","obj":"T25207"},{"id":"R19266","pred":"punct","subj":"T25210","obj":"T25194"},{"id":"R19267","pred":"nsubj","subj":"T25211","obj":"T25212"},{"id":"R19268","pred":"ROOT","subj":"T25212","obj":"T25212"},{"id":"R19269","pred":"det","subj":"T25213","obj":"T25216"},{"id":"R19270","pred":"amod","subj":"T25214","obj":"T25216"},{"id":"R19271","pred":"compound","subj":"T25215","obj":"T25216"},{"id":"R19272","pred":"dobj","subj":"T25216","obj":"T25212"},{"id":"R19273","pred":"prep","subj":"T25217","obj":"T25212"},{"id":"R19274","pred":"nummod","subj":"T25218","obj":"T25220"},{"id":"R19275","pred":"amod","subj":"T25219","obj":"T25220"},{"id":"R19276","pred":"pobj","subj":"T25220","obj":"T25217"},{"id":"R19277","pred":"punct","subj":"T25221","obj":"T25212"}],"text":"10.1371/journal.pone.0028081.g006 Figure 6 Inhibition of M-CSF-induced PKC reduces NF-κB-regulated genes in both MDMs and RAW 264.7 cells.\nMDMs (A and C) and RAW 264.7 (B) cells were pretreated with Ro-31-8220 or solvent control DMSO for 30 minutes prior to M-CSF stimulation for the indicated times. Total RNA was isolated and converted to cDNA. Real-time RT PCR was performed using primers for IκBα, BCL-xl or GAPDH. Data are expressed as relative fold increase of IκBα or BCL-xl gene expression upon treatment over non-stimulated cells. Data represent the mean ± S.E.M for three independent experiments. "}
test2
{"project":"test2","denotations":[{"id":"T25107","span":{"begin":58,"end":63},"obj":"Protein"},{"id":"T25108","span":{"begin":259,"end":264},"obj":"Protein"},{"id":"T25109","span":{"begin":397,"end":401},"obj":"Protein"},{"id":"T25110","span":{"begin":403,"end":409},"obj":"Protein"},{"id":"T25111","span":{"begin":456,"end":464},"obj":"Positive_regulation"},{"id":"T25112","span":{"begin":468,"end":472},"obj":"Protein"},{"id":"T25113","span":{"begin":476,"end":482},"obj":"Protein"},{"id":"T25114","span":{"begin":488,"end":498},"obj":"Gene_expression"}],"relations":[{"id":"R19174","pred":"themeOf","subj":"T25112","obj":"T25114"},{"id":"R19175","pred":"themeOf","subj":"T25113","obj":"T25114"},{"id":"R19176","pred":"themeOf","subj":"T25114","obj":"T25111"}],"text":"10.1371/journal.pone.0028081.g006 Figure 6 Inhibition of M-CSF-induced PKC reduces NF-κB-regulated genes in both MDMs and RAW 264.7 cells.\nMDMs (A and C) and RAW 264.7 (B) cells were pretreated with Ro-31-8220 or solvent control DMSO for 30 minutes prior to M-CSF stimulation for the indicated times. Total RNA was isolated and converted to cDNA. Real-time RT PCR was performed using primers for IκBα, BCL-xl or GAPDH. Data are expressed as relative fold increase of IκBα or BCL-xl gene expression upon treatment over non-stimulated cells. Data represent the mean ± S.E.M for three independent experiments. "}