PMC:3245220 / 22158-22767 JSONTXT

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    bionlp-st-ge-2016-uniprot

    {"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T25317","span":{"begin":58,"end":63},"obj":"P09603"},{"id":"T25318","span":{"begin":60,"end":63},"obj":"P04141"},{"id":"T25319","span":{"begin":259,"end":264},"obj":"P09603"},{"id":"T25320","span":{"begin":261,"end":264},"obj":"P04141"},{"id":"T25321","span":{"begin":397,"end":401},"obj":"P25963"},{"id":"T25322","span":{"begin":413,"end":418},"obj":"P04406"},{"id":"T25323","span":{"begin":442,"end":450},"obj":"Q04864"},{"id":"T25324","span":{"begin":468,"end":472},"obj":"P25963"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"10.1371/journal.pone.0028081.g006 Figure 6 Inhibition of M-CSF-induced PKC reduces NF-κB-regulated genes in both MDMs and RAW 264.7 cells.\nMDMs (A and C) and RAW 264.7 (B) cells were pretreated with Ro-31-8220 or solvent control DMSO for 30 minutes prior to M-CSF stimulation for the indicated times. Total RNA was isolated and converted to cDNA. Real-time RT PCR was performed using primers for IκBα, BCL-xl or GAPDH. Data are expressed as relative fold increase of IκBα or BCL-xl gene expression upon treatment over non-stimulated cells. Data represent the mean ± S.E.M for three independent experiments. "}

    bionlp-st-ge-2016-reference

    {"project":"bionlp-st-ge-2016-reference","denotations":[{"id":"T25325","span":{"begin":456,"end":464},"obj":"Positive_regulation"},{"id":"T25326","span":{"begin":488,"end":498},"obj":"Gene_expression"},{"id":"T25327","span":{"begin":456,"end":464},"obj":"Positive_regulation"},{"id":"T25328","span":{"begin":488,"end":498},"obj":"Gene_expression"},{"id":"T25329","span":{"begin":397,"end":401},"obj":"Protein"},{"id":"T25330","span":{"begin":403,"end":409},"obj":"Protein"},{"id":"T25331","span":{"begin":468,"end":472},"obj":"Protein"},{"id":"T25332","span":{"begin":476,"end":482},"obj":"Protein"},{"id":"T25333","span":{"begin":58,"end":63},"obj":"Protein"},{"id":"T25334","span":{"begin":259,"end":264},"obj":"Protein"},{"id":"T25335","span":{"begin":44,"end":54},"obj":"Negative_regulation"}],"relations":[{"id":"R19373","pred":"themeOf","subj":"T25326","obj":"T25325"},{"id":"R19374","pred":"themeOf","subj":"T25328","obj":"T25327"},{"id":"R19375","pred":"themeOf","subj":"T25331","obj":"T25326"},{"id":"R19376","pred":"themeOf","subj":"T25332","obj":"T25328"},{"id":"R19377","pred":"themeOf","subj":"T25333","obj":"T25335"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"10.1371/journal.pone.0028081.g006 Figure 6 Inhibition of M-CSF-induced PKC reduces NF-κB-regulated genes in both MDMs and RAW 264.7 cells.\nMDMs (A and C) and RAW 264.7 (B) cells were pretreated with Ro-31-8220 or solvent control DMSO for 30 minutes prior to M-CSF stimulation for the indicated times. Total RNA was isolated and converted to cDNA. Real-time RT PCR was performed using primers for IκBα, BCL-xl or GAPDH. Data are expressed as relative fold increase of IκBα or BCL-xl gene expression upon treatment over non-stimulated cells. Data represent the mean ± S.E.M for three independent experiments. "}

    bionlp-st-ge-2016-reference-tees

    {"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T25409","span":{"begin":58,"end":63},"obj":"Protein"},{"id":"T25410","span":{"begin":72,"end":75},"obj":"Protein"},{"id":"T25411","span":{"begin":84,"end":89},"obj":"Protein"},{"id":"T25412","span":{"begin":64,"end":71},"obj":"Positive_regulation"},{"id":"T25413","span":{"begin":44,"end":54},"obj":"Negative_regulation"},{"id":"T25414","span":{"begin":259,"end":264},"obj":"Protein"},{"id":"T25415","span":{"begin":397,"end":409},"obj":"Protein"},{"id":"T25416","span":{"begin":413,"end":418},"obj":"Protein"},{"id":"T25417","span":{"begin":468,"end":472},"obj":"Protein"},{"id":"T25418","span":{"begin":476,"end":479},"obj":"Protein"},{"id":"T25419","span":{"begin":488,"end":498},"obj":"Gene_expression"},{"id":"T25420","span":{"begin":488,"end":498},"obj":"Gene_expression"},{"id":"T25421","span":{"begin":456,"end":464},"obj":"Positive_regulation"},{"id":"T25422","span":{"begin":456,"end":464},"obj":"Positive_regulation"}],"relations":[{"id":"R19406","pred":"causeOf","subj":"T25409","obj":"T25412"},{"id":"R19407","pred":"themeOf","subj":"T25410","obj":"T25412"},{"id":"R19408","pred":"themeOf","subj":"T25412","obj":"T25413"},{"id":"R19409","pred":"themeOf","subj":"T25417","obj":"T25419"},{"id":"R19410","pred":"themeOf","subj":"T25418","obj":"T25420"},{"id":"R19411","pred":"themeOf","subj":"T25419","obj":"T25421"},{"id":"R19412","pred":"themeOf","subj":"T25420","obj":"T25422"}],"text":"10.1371/journal.pone.0028081.g006 Figure 6 Inhibition of M-CSF-induced PKC reduces NF-κB-regulated genes in both MDMs and RAW 264.7 cells.\nMDMs (A and C) and RAW 264.7 (B) cells were pretreated with Ro-31-8220 or solvent control DMSO for 30 minutes prior to M-CSF stimulation for the indicated times. Total RNA was isolated and converted to cDNA. Real-time RT PCR was performed using primers for IκBα, BCL-xl or GAPDH. Data are expressed as relative fold increase of IκBα or BCL-xl gene expression upon treatment over non-stimulated cells. Data represent the mean ± S.E.M for three independent experiments. "}

    events-check-again

    {"project":"events-check-again","denotations":[{"id":"T25398","span":{"begin":44,"end":54},"obj":"Negative_regulation"},{"id":"T25399","span":{"begin":58,"end":63},"obj":"Protein"},{"id":"T25400","span":{"begin":259,"end":264},"obj":"Protein"},{"id":"T25401","span":{"begin":397,"end":401},"obj":"Protein"},{"id":"T25402","span":{"begin":403,"end":409},"obj":"Protein"},{"id":"T25403","span":{"begin":456,"end":464},"obj":"Positive_regulation"},{"id":"T25404","span":{"begin":456,"end":464},"obj":"Positive_regulation"},{"id":"T25405","span":{"begin":468,"end":472},"obj":"Protein"},{"id":"T25406","span":{"begin":476,"end":482},"obj":"Protein"},{"id":"T25407","span":{"begin":488,"end":498},"obj":"Gene_expression"},{"id":"T25408","span":{"begin":488,"end":498},"obj":"Gene_expression"}],"relations":[{"id":"R19401","pred":"themeOf","subj":"T25399","obj":"T25398"},{"id":"R19402","pred":"themeOf","subj":"T25405","obj":"T25407"},{"id":"R19403","pred":"themeOf","subj":"T25406","obj":"T25408"},{"id":"R19404","pred":"themeOf","subj":"T25407","obj":"T25403"},{"id":"R19405","pred":"themeOf","subj":"T25408","obj":"T25404"}],"text":"10.1371/journal.pone.0028081.g006 Figure 6 Inhibition of M-CSF-induced PKC reduces NF-κB-regulated genes in both MDMs and RAW 264.7 cells.\nMDMs (A and C) and RAW 264.7 (B) cells were pretreated with Ro-31-8220 or solvent control DMSO for 30 minutes prior to M-CSF stimulation for the indicated times. Total RNA was isolated and converted to cDNA. Real-time RT PCR was performed using primers for IκBα, BCL-xl or GAPDH. Data are expressed as relative fold increase of IκBα or BCL-xl gene expression upon treatment over non-stimulated cells. Data represent the mean ± S.E.M for three independent experiments. "}

    GO-CC

    {"project":"GO-CC","denotations":[{"id":"T25344","span":{"begin":133,"end":138},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T25345","span":{"begin":173,"end":178},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T25346","span":{"begin":534,"end":539},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"10.1371/journal.pone.0028081.g006 Figure 6 Inhibition of M-CSF-induced PKC reduces NF-κB-regulated genes in both MDMs and RAW 264.7 cells.\nMDMs (A and C) and RAW 264.7 (B) cells were pretreated with Ro-31-8220 or solvent control DMSO for 30 minutes prior to M-CSF stimulation for the indicated times. Total RNA was isolated and converted to cDNA. Real-time RT PCR was performed using primers for IκBα, BCL-xl or GAPDH. Data are expressed as relative fold increase of IκBα or BCL-xl gene expression upon treatment over non-stimulated cells. Data represent the mean ± S.E.M for three independent experiments. "}

    GO-MF

    {"project":"GO-MF","denotations":[{"id":"T25342","span":{"begin":72,"end":75},"obj":"http://purl.obolibrary.org/obo/GO_0004697"},{"id":"T25343","span":{"begin":358,"end":360},"obj":"http://purl.obolibrary.org/obo/GO_0003964"}],"text":"10.1371/journal.pone.0028081.g006 Figure 6 Inhibition of M-CSF-induced PKC reduces NF-κB-regulated genes in both MDMs and RAW 264.7 cells.\nMDMs (A and C) and RAW 264.7 (B) cells were pretreated with Ro-31-8220 or solvent control DMSO for 30 minutes prior to M-CSF stimulation for the indicated times. Total RNA was isolated and converted to cDNA. Real-time RT PCR was performed using primers for IκBα, BCL-xl or GAPDH. Data are expressed as relative fold increase of IκBα or BCL-xl gene expression upon treatment over non-stimulated cells. Data represent the mean ± S.E.M for three independent experiments. "}

    GO-BP

    {"project":"GO-BP","denotations":[{"id":"T25423","span":{"begin":358,"end":360},"obj":"http://purl.obolibrary.org/obo/GO_0003964"},{"id":"T25424","span":{"begin":483,"end":498},"obj":"http://purl.obolibrary.org/obo/GO_0010467"}],"text":"10.1371/journal.pone.0028081.g006 Figure 6 Inhibition of M-CSF-induced PKC reduces NF-κB-regulated genes in both MDMs and RAW 264.7 cells.\nMDMs (A and C) and RAW 264.7 (B) cells were pretreated with Ro-31-8220 or solvent control DMSO for 30 minutes prior to M-CSF stimulation for the indicated times. Total RNA was isolated and converted to cDNA. Real-time RT PCR was performed using primers for IκBα, BCL-xl or GAPDH. Data are expressed as relative fold increase of IκBα or BCL-xl gene expression upon treatment over non-stimulated cells. Data represent the mean ± S.E.M for three independent experiments. "}

    pmc-enju-pas

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Figure 6 Inhibition of M-CSF-induced PKC reduces NF-κB-regulated genes in both MDMs and RAW 264.7 cells.\nMDMs (A and C) and RAW 264.7 (B) cells were pretreated with Ro-31-8220 or solvent control DMSO for 30 minutes prior to M-CSF stimulation for the indicated times. 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