Effects of rs2227473 genotypes on IL-22's protein expression
To test whether different genotypes of the rs2227473 affect IL-22's expression levels, we stimulated patients' peripheral blood mononuclear cells (PBMCs) with both anti-CD3/anti-CD28 antigene nonspecific and Mtb antigen-specific stimulations. Patients with A allele (GA+AA, n = 29) at rs2227473 had significantly higher IL-22 protein productions than those without A allele (GG, n = 29) under both non-specific (p value = 0.0095) and specific stimulations (p value = 0.0099, Figure 1).