IL-22 production by PBMCs from individuals with polymorphism in IL-22 (rs2227473).
Subjects were grouped for SNP (rs2227473) in IL-22 as genotype with (GA+AA, n = 29) or without (GG, n = 29) A allele. Frozen PBMCs were thawed and cultured for 96 hours in the presence of anti-CD3/anti-CD28 (1µg/ml each), or killed Mtb. (20µg/ml). Supernatant was collected and the concentration of IL-22 was determined by ELISA. IL-22 protein production by PBMC in response to (A) anti-CD3/anti-CD28 and (B) Mtb. Data are represented as mean ± SEM. P values are indicated.