Quantifications of IL-22 under specific and non-specific stimuli of PBMCs
5ml peripheral blood samples of TB patients were collected and PBMCs were isolated by the Ficoll lymphocyte separation medium. PBMCs were then spread into a 96-well plate at the cell density of 4×105, and 1ug/ml of monoclonal antibodies anti-CD3 and anti-CD28 were added for non-specific stimulation, and 20ug/ml of Mycobacterium tuberculosis (Mtb) lysate were added for specific stimulation. The stimulated PBMCs were incubated at 37°C, 5% CO2 for 96h. Cell supernatants were used for IL-22 protein quantification with ELISA.