(A,B) Immunostaining of the brains of Zic2+/+ (+/+) (A) and Zic2kd/+ (kd/+) (B) mice with anti-ChAT antibody. Coronal sections through the septum and diagonal bands derived from adult male mice were subjected to immunoperoxidase staining. Scale bar, 1 mm. (C) Number of the ChAT+ neurons in the sections. Mean numbers of ChAT+ neurons in 20 sections from the Zic2+/+ and Zic2kd/+ mice brains are indicated. (D) PV-positive cell numbers. The measurements were taken in comparable regions to those subjected to ChAT-immunostaining. (E) The numbers of ChAT- and Zic-immunoreactive neurons in early postnatal (P5-7) Zic2+/+ and Zic2kd/+ brains. Double labeling was performed with the anti-ChAT antibody and anti-pan Zic antibody. CC, cerebral cortex; CP, caudoputamen; DB, diagonal band; LV, lateral ventricle; LS, lateral septum; MS, medial septum; MY, Mynert nucleus; SI, substantia innominata; STR, striatum. (C–E) Data is presented as means ± SEM. The number of mice in each group is given in parentheses. *P < 0.05, ***P < 0.001 in t-test.