Electronic supplementary material

Additional file 1: All IFNG.bio. An alignment of IFNG gene sequences from Homo sapiens, Pterops vampyrus, and Myotis lucifugus as follows: first row: genomic sequence of human IFNG. rows 2, 3: predicted genomic sequence of IFNG in M. lucifugus and P. vampyrus, respectively. rows 4-7: exons for human IFNG. rows 8-11: predicted exons for M. lucifugus. rows 12-15: predicted exons for P. vampyrus. row 16: spliced coding region for human. rows 17,18: predicted spliced coding region for M. lucifugus and P. vampyrus. row 19: amino acid sequence for human interferon gamma. rows 20,21: predicted amino acid sequence for chiropteran interferon gamma. Examination of this. bio file requires the BioEdit program, which is freely available at http://www.mbio.ncsu.edu/BioEdit/bioedit.html. (BIO 24 KB)
Additional file 2: Pva IFND clone-assembly comparison.fasta. This sequence alignment shows a comparison between Pteropus vampyrus IFND sequences obtained by 1) assembly from the genome sequencing trace archives, and 2) by direct cloning and sequencing. The cloned sequences include only those found in at least two independent clones. The assemblies include all those with intact ORFs plus three that appear to be pseudogenes. (FAST 6 KB)
Additional file 3: Accession numbers and primers. The Genbank accession numbers of the IFN genes used in the phylogenetic. analysis and the PCR primers used in the P. vampyrus gene expression studies. (DOCX 18 KB)
Additional file 4: Mlu + Pva + Hsa + SscSelectedIntactOrfsAA-aligned.fasta. The amino acid sequences used in the phylogenetic analysis. (FAST 21 KB)