PMC:3062687 / 19647-23992 JSON TXT

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bionlp-st-ge-2016-uniprot

Id	Subject	Object	Predicate	Lexical cue
T15646	0-5	P23396	denotes	NleH1
T15647	17-21	O14920	denotes	IKKβ
T15648	46-51	P23396	denotes	NleH1
T15649	177-182	P23396	denotes	NleH1
T15650	192-196	P23396	denotes	RPS3
T15651	291-296	P23396	denotes	NleH1
T15652	322-327	P23396	denotes	NleH1
T15653	361-366	P23396	denotes	NleH1
T15654	409-414	P23396	denotes	NleH1
T15655	500-505	P23396	denotes	NleH1
T15656	517-521	O14920	denotes	IKKβ
T15657	541-545	P23396	denotes	RPS3
T15658	607-612	P23396	denotes	NleH1
T15659	638-643	P23396	denotes	NleH1
T15660	677-680	P01375	denotes	TNF
T15661	689-693	P23396	denotes	RPS3
T15662	773-778	P23396	denotes	NleH1
T15663	818-822	P23396	denotes	RPS3
T15664	828-832	O14920	denotes	IKKβ
T15665	1016-1020	P23396	denotes	RPS3
T15666	1047-1051	P23396	denotes	RPS3
T15667	1068-1078	P08659	denotes	luciferase
T15668	1123-1128	P23396	denotes	NleH1
T15669	1150-1154	P23396	denotes	RPS3
T15670	1257-1260	P01375	denotes	TNF
T15671	1306-1310	P23396	denotes	RPS3
T15672	1364-1368	P23396	denotes	RPS3
T15673	1466-1470	P23396	denotes	RPS3
T15674	1615-1620	P23396	denotes	NleH1
T15675	1743-1748	P23396	denotes	NleH1
T15676	1792-1797	P23396	denotes	NleH1
T15677	1815-1818	P01375	denotes	TNF
T15678	1827-1831	P23396	denotes	RPS3
T15679	1904-1908	P23396	denotes	RPS3
T15680	1981-1986	P23396	denotes	NleH1
T15681	2024-2028	P23396	denotes	RPS3
T15682	2103-2108	P23396	denotes	NleH1
T15683	2178-2182	P23396	denotes	RPS3
T15684	2220-2224	O14920	denotes	IKKβ
T15685	2226-2230	O14920	denotes	IKKβ
T15686	2312-2316	O14920	denotes	IKKβ
T15687	2342-2346	P23396	denotes	RPS3
T15688	2390-2394	O14920	denotes	IKKβ
T15689	2421-2425	P23396	denotes	RPS3
T15690	2570-2576	P23396	denotes	ΔnleH1
T15691	2580-2585	Q7DB71	denotes	ΔescN
T15692	2617-2621	P23396	denotes	RPS3
T15693	2654-2658	O14920	denotes	IKKβ
T15694	2663-2667	O14920	denotes	IKKβ
T15695	2754-2758	P23396	denotes	RPS3
T15696	2784-2788	O14920	denotes	IKKβ
T15697	2878-2883	P23396	denotes	NleH1
T15698	2918-2922	P23396	denotes	RPS3
T15699	2995-2999	O14920	denotes	IKKβ
T15700	3021-3026	P23396	denotes	NleH1
T15701	3056-3060	O14920	denotes	IKKβ
T15702	3077-3081	O14920	denotes	IKKβ
T15703	3091-3095	P23396	denotes	RPS3
T15704	3184-3188	O14920	denotes	IKKβ
T15705	3229-3234	P23396	denotes	NleH1
T15706	3254-3258	O14920	denotes	IKKβ
T15707	3297-3302	P23396	denotes	NleH1
T15708	3392-3396	O14920	denotes	IKKβ
T15709	3491-3496	P23396	denotes	NleH1
T15710	3513-3517	O14920	denotes	IKKβ
T15711	3630-3634	O14920	denotes	IKKβ
T15712	3649-3653	O14920	denotes	IKKβ
T15713	3669-3673	P23396	denotes	RPS3
T15714	3700-3704	P25963	denotes	IκBα
T15715	3822-3826	O14920	denotes	IKKβ
T15716	3832-3837	P23396	denotes	NleH1
T15717	3846-3850	O14920	denotes	IKKβ
T15718	3860-3864	P23396	denotes	RPS3
T15719	3923-3927	O14920	denotes	IKKβ
T15720	3941-3945	P25963	denotes	IκBα
T15721	4038-4043	P23396	denotes	NleH1
T15722	4095-4099	P23396	denotes	RPS3
T15723	4107-4111	P25963	denotes	IκBα
T15724	4139-4144	P23396	denotes	NleH1
T15725	4185-4189	O14920	denotes	IKKβ
T15726	4210-4214	O14920	denotes	IKKβ
T15727	4224-4228	P23396	denotes	RPS3

2_test

Id	Subject	Object	Predicate	Lexical cue
21399639-20041225-74765175	140-141	20041225	denotes	9
21399639-20041225-74765175	140-141	20041225	denotes	9
21399639-12657048-74765176	948-950	12657048	denotes	39
21399639-12657048-74765176	948-950	12657048	denotes	39
21399639-20041225-74765177	1135-1136	20041225	denotes	9
21399639-20041225-74765177	1135-1136	20041225	denotes	9

pmc-enju-pas

Id	Subject	Object	Predicate	Lexical cue
T14934	0-5	NN	denotes	NleH1
T14935	6-12	NN	denotes	steers
T14936	13-16	DT	denotes	the
T14937	17-21	NN	denotes	IKKβ
T14938	22-31	NN	denotes	substrate
T14939	32-45	NN	denotes	specificities
T14940	46-51	NN	denotes	NleH1
T14941	52-54	VB	denotes	is
T14942	55-57	DT	denotes	an
T14943	58-76	VB	denotes	autophosphorylated
T14944	77-93	NN	denotes	serine-threonine
T14945	94-100	NN	denotes	kinase
T14946	100-101	-COMMA-	denotes	,
T14947	102-107	WDT	denotes	which
T14948	108-115	VB	denotes	depends
T14949	116-118	IN	denotes	on
T14950	119-122	DT	denotes	the
T14951	123-129	NN	denotes	lysine
T14952	130-133	CD	denotes	159
T14953	134-135	-LRB-	denotes	(
T14954	135-139	NN	denotes	K159
T14955	139-140	-RRB-	denotes	)
T14956	140-141	CD	denotes	9
T14957	143-145	TO	denotes	To
T14958	146-153	VB	denotes	explore
T14959	154-157	DT	denotes	the
T14960	158-167	NN	denotes	mechanism
T14961	168-170	IN	denotes	by
T14962	171-176	WDT	denotes	which
T14963	177-182	NN	denotes	NleH1
T14964	183-191	VB	denotes	inhibits
T14965	192-196	NN	denotes	RPS3
T14966	197-201	NN	denotes	S209
T14967	202-217	NN	denotes	phosphorylation
T14968	217-218	-COMMA-	denotes	,
T14969	219-221	PRP	denotes	we
T14970	222-227	RB	denotes	first
T14971	228-237	VB	denotes	performed
T14972	238-240	DT	denotes	an
T14973	241-243	FW	denotes	in
T14974	244-249	FW	denotes	vitro
T14975	250-256	NN	denotes	kinase
T14976	257-262	NN	denotes	assay
T14977	263-267	IN	denotes	with
T14978	268-276	VB	denotes	purified
T14979	277-286	JJ	denotes	wild-type
T14980	287-296	NN	denotes	His-NleH1
T14981	297-304	NN	denotes	protein
T14982	305-308	CC	denotes	and
T14983	309-310	DT	denotes	a
T14984	311-317	JJ	denotes	mutant
T14985	318-327	NN	denotes	His-NleH1
T14986	328-329	-LRB-	denotes	(
T14987	329-334	NN	denotes	K159A
T14988	334-335	-RRB-	denotes	)
T14989	336-343	NN	denotes	protein
T14990	343-344	-COMMA-	denotes	,
T14991	345-355	VB	denotes	confirming
T14992	356-360	IN	denotes	that
T14993	361-366	NN	denotes	NleH1
T14994	367-369	VB	denotes	is
T14995	370-388	VB	denotes	autophosphorylated
T14996	389-392	CC	denotes	and
T14997	393-396	DT	denotes	the
T14998	397-402	NN	denotes	K159A
T14999	403-405	VB	denotes	is
T15000	406-408	DT	denotes	an
T15001	409-414	NN	denotes	NleH1
T15002	415-426	NN	denotes	kinase-dead
T15003	427-433	NN	denotes	mutant
T15004	434-435	-LRB-	denotes	(
T15005	435-439	NN	denotes	Fig.
T15006	440-442	NN	denotes	7a
T15007	442-443	-RRB-	denotes	)
T15008	445-447	TO	denotes	To
T15009	448-455	VB	denotes	examine
T15010	456-463	IN	denotes	whether
T15011	464-467	DT	denotes	the
T15012	468-474	NN	denotes	kinase
T15013	475-483	NN	denotes	activity
T15014	484-486	VB	denotes	is
T15015	487-495	VB	denotes	required
T15016	496-499	IN	denotes	for
T15017	500-505	NN	denotes	NleH1
T15018	506-508	TO	denotes	to
T15019	509-516	VB	denotes	inhibit
T15020	517-521	NN	denotes	IKKβ
T15021	522-537	NN	denotes	phosphorlyation
T15022	538-540	IN	denotes	of
T15023	541-545	NN	denotes	RPS3
T15024	546-548	IN	denotes	on
T15025	549-553	NN	denotes	S209
T15026	553-554	-COMMA-	denotes	,
T15027	555-557	PRP	denotes	we
T15028	558-569	RB	denotes	ectopically
T15029	570-580	VB	denotes	expressing
T15030	581-587	CC	denotes	either
T15031	588-597	JJ	denotes	wild-type
T15032	598-600	CC	denotes	or
T15033	601-606	NN	denotes	K159A
T15034	607-612	NN	denotes	NleH1
T15035	613-615	IN	denotes	in
T15036	616-620	NN	denotes	293T
T15037	621-626	NN	denotes	cells
T15038	628-637	JJ	denotes	Wild-type
T15039	638-643	NN	denotes	NleH1
T15040	644-654	NN	denotes	expression
T15041	655-668	RB	denotes	significantly
T15042	669-676	VB	denotes	reduced
T15043	677-688	JJ	denotes	TNF-induced
T15044	689-693	NN	denotes	RPS3
T15045	694-698	NN	denotes	S209
T15046	699-714	NN	denotes	phosphorylation
T15047	714-715	-COMMA-	denotes	,
T15048	716-723	IN	denotes	whereas
T15049	724-727	DT	denotes	the
T15050	728-733	NN	denotes	K159A
T15051	734-740	NN	denotes	mutant
T15052	741-747	VB	denotes	failed
T15053	748-750	TO	denotes	to
T15054	751-753	VB	denotes	do
T15055	754-756	RB	denotes	so
T15056	757-758	-LRB-	denotes	(
T15057	758-762	NNP	denotes	Fig.
T15058	763-765	NN	denotes	7b
T15059	765-766	-RRB-	denotes	)
T15060	768-772	RB	denotes	Thus
T15061	773-778	NN	denotes	NleH1
T15062	779-785	NN	denotes	kinase
T15063	786-794	NN	denotes	activity
T15064	795-797	VB	denotes	is
T15065	798-806	VB	denotes	required
T15066	807-809	TO	denotes	to
T15067	810-817	VB	denotes	protect
T15068	818-822	NN	denotes	RPS3
T15069	823-827	IN	denotes	from
T15070	828-841	JJ	denotes	IKKβ-mediated
T15071	842-857	NN	denotes	phosphorylation
T15072	859-870	NN	denotes	Citrobacter
T15073	871-880	NN	denotes	rodentium
T15074	881-883	VB	denotes	is
T15075	884-885	DT	denotes	a
T15076	886-891	NN	denotes	mouse
T15077	892-900	NN	denotes	pathogen
T15078	901-905	WDT	denotes	that
T15079	906-912	VB	denotes	shares
T15080	913-923	JJ	denotes	pathogenic
T15081	924-934	NN	denotes	strategies
T15082	935-939	IN	denotes	with
T15083	940-942	FW	denotes	E.
T15084	943-947	FW	denotes	coli
T15085	948-950	CD	denotes	39
T15086	950-951	-COMMA-	denotes	,
T15087	952-956	RB	denotes	most
T15088	957-964	RB	denotes	notably
T15089	965-968	IN	denotes	for
T15090	969-972	PRP-DOLLAR-	denotes	our
T15091	973-986	NN	denotes	investigation
T15092	986-987	-COMMA-	denotes	,
T15093	988-990	NNP	denotes	C.
T15094	991-1000	NN	denotes	rodentium
T15095	1001-1005	NN	denotes	NleH
T15096	1006-1015	VB	denotes	inhibited
T15097	1016-1020	NN	denotes	RPS3
T15098	1021-1028	JJ	denotes	nuclear
T15099	1029-1042	NN	denotes	translocation
T15100	1043-1046	CC	denotes	and
T15101	1047-1061	JJ	denotes	RPS3-dependent
T15102	1062-1067	NN	denotes	NF-κB
T15103	1068-1078	NN	denotes	luciferase
T15104	1079-1087	NN	denotes	activity
T15105	1088-1090	TO	denotes	to
T15106	1091-1093	DT	denotes	an
T15107	1094-1100	NN	denotes	extent
T15108	1101-1111	JJ	denotes	equivalent
T15109	1112-1114	TO	denotes	to
T15110	1115-1117	FW	denotes	E.
T15111	1118-1122	FW	denotes	coli
T15112	1123-1128	NN	denotes	NleH1
T15113	1129-1130	-LRB-	denotes	(
T15114	1130-1133	NN	denotes	ref
T15115	1135-1136	CD	denotes	9
T15116	1136-1137	-RRB-	denotes	)
T15117	1139-1141	PRP	denotes	We
T15118	1142-1149	VB	denotes	assayed
T15119	1150-1154	NN	denotes	RPS3
T15120	1155-1159	NN	denotes	S209
T15121	1160-1175	NN	denotes	phosphorylation
T15122	1176-1178	IN	denotes	in
T15123	1179-1183	NN	denotes	HeLa
T15124	1184-1189	NN	denotes	cells
T15125	1190-1198	VB	denotes	infected
T15126	1199-1203	IN	denotes	with
T15127	1204-1213	JJ	denotes	different
T15128	1214-1216	NNP	denotes	C.
T15129	1217-1226	NN	denotes	rodentium
T15130	1227-1234	NN	denotes	strains
T15131	1236-1238	IN	denotes	In
T15132	1239-1249	JJ	denotes	uninfected
T15133	1250-1255	NN	denotes	cells
T15134	1255-1256	-COMMA-	denotes	,
T15135	1257-1270	NN	denotes	TNF-treatment
T15136	1271-1281	VB	denotes	stimulated
T15137	1282-1283	DT	denotes	a
T15138	1284-1293	JJ	denotes	∼3.5-fold
T15139	1294-1302	NN	denotes	increase
T15140	1303-1305	IN	denotes	in
T15141	1306-1310	NN	denotes	RPS3
T15142	1311-1315	NN	denotes	S209
T15143	1316-1331	NN	denotes	phosphorylation
T15144	1332-1333	-LRB-	denotes	(
T15145	1333-1337	NN	denotes	Fig.
T15146	1338-1340	NN	denotes	7c
T15147	1340-1341	-RRB-	denotes	)
T15148	1343-1347	JJ	denotes	Such
T15149	1348-1360	NN	denotes	augmentation
T15150	1361-1363	IN	denotes	of
T15151	1364-1368	NN	denotes	RPS3
T15152	1369-1384	NN	denotes	phosphorylation
T15153	1385-1388	VB	denotes	was
T15154	1389-1396	VB	denotes	reduced
T15155	1397-1399	IN	denotes	by
T15156	1400-1405	RB	denotes	about
T15157	1406-1408	CD	denotes	60
T15158	1408-1409	NN	denotes	%
T15159	1410-1412	IN	denotes	by
T15160	1413-1422	JJ	denotes	wild-type
T15161	1423-1425	FW	denotes	C.
T15162	1426-1435	NN	denotes	rodentium
T15163	1436-1445	NN	denotes	infection
T15164	1446-1447	-LRB-	denotes	(
T15165	1447-1451	NN	denotes	Fig.
T15166	1452-1454	NN	denotes	7c
T15167	1454-1455	-RRB-	denotes	)
T15168	1457-1464	RB	denotes	However
T15169	1464-1465	-COMMA-	denotes	,
T15170	1466-1470	NN	denotes	RPS3
T15171	1471-1486	NN	denotes	phosphorylation
T15172	1487-1490	VB	denotes	was
T15173	1491-1499	VB	denotes	enhanced
T15174	1500-1504	WRB	denotes	when
T15175	1505-1510	NN	denotes	cells
T15176	1511-1515	VB	denotes	were
T15177	1516-1524	VB	denotes	infected
T15178	1525-1529	IN	denotes	with
T15179	1530-1531	DT	denotes	a
T15180	1532-1534	NNP	denotes	C.
T15181	1535-1544	NN	denotes	rodentium
T15182	1545-1551	NN	denotes	strain
T15183	1552-1559	VB	denotes	lacking
T15184	1560-1564	NN	denotes	NleH
T15185	1565-1566	-LRB-	denotes	(
T15186	1566-1570	NNP	denotes	Fig.
T15187	1571-1573	NN	denotes	7c
T15188	1573-1574	-COMMA-	denotes	,
T15189	1575-1580	NN	denotes	ΔnleH
T15190	1580-1581	-RRB-	denotes	)
T15191	1583-1585	PRP	denotes	We
T15192	1586-1593	RB	denotes	further
T15193	1594-1602	VB	denotes	examined
T15194	1603-1606	DT	denotes	the
T15195	1607-1611	NN	denotes	role
T15196	1612-1614	IN	denotes	of
T15197	1615-1620	NN	denotes	NleH1
T15198	1621-1627	NN	denotes	kinase
T15199	1628-1636	NN	denotes	activity
T15200	1637-1642	VB	denotes	using
T15201	1643-1646	DT	denotes	the
T15202	1647-1649	NNP	denotes	C.
T15203	1650-1659	NN	denotes	rodentium
T15204	1660-1665	NN	denotes	ΔnleH
T15205	1666-1672	NN	denotes	strain
T15206	1673-1675	IN	denotes	as
T15207	1676-1677	DT	denotes	a
T15208	1678-1688	NN	denotes	background
T15209	1689-1691	IN	denotes	on
T15210	1692-1697	WDT	denotes	which
T15211	1698-1700	TO	denotes	to
T15212	1701-1708	VB	denotes	express
T15213	1709-1715	CC	denotes	either
T15214	1716-1725	JJ	denotes	wild-type
T15215	1726-1728	CC	denotes	or
T15216	1729-1734	NN	denotes	K159A
T15217	1735-1737	FW	denotes	E.
T15218	1738-1742	FW	denotes	coli
T15219	1743-1748	NN	denotes	NleH1
T15220	1750-1763	VB	denotes	Complementing
T15221	1764-1769	NN	denotes	ΔnleH
T15222	1770-1776	NN	denotes	mutant
T15223	1777-1781	IN	denotes	with
T15224	1782-1791	JJ	denotes	wild-type
T15225	1792-1797	NN	denotes	NleH1
T15226	1798-1804	RB	denotes	almost
T15227	1805-1814	VB	denotes	abolished
T15228	1815-1826	JJ	denotes	TNF-induced
T15229	1827-1831	NN	denotes	RPS3
T15230	1832-1836	NN	denotes	S209
T15231	1837-1852	NN	denotes	phosphorylation
T15232	1853-1860	IN	denotes	whereas
T15233	1861-1874	VB	denotes	complementing
T15234	1875-1879	IN	denotes	with
T15235	1880-1885	NN	denotes	K159A
T15236	1886-1892	VB	denotes	failed
T15237	1893-1895	TO	denotes	to
T15238	1896-1903	VB	denotes	inhibit
T15239	1904-1908	NN	denotes	RPS3
T15240	1909-1924	NN	denotes	phosphorylation
T15241	1925-1926	-LRB-	denotes	(
T15242	1926-1930	NN	denotes	Fig.
T15243	1931-1933	NN	denotes	7c
T15244	1933-1934	-RRB-	denotes	)
T15245	1936-1948	RB	denotes	Collectively
T15246	1948-1949	-COMMA-	denotes	,
T15247	1950-1955	DT	denotes	these
T15248	1956-1963	NN	denotes	results
T15249	1964-1975	VB	denotes	demonstrate
T15250	1976-1980	IN	denotes	that
T15251	1981-1986	NN	denotes	NleH1
T15252	1987-1993	NN	denotes	kinase
T15253	1994-2002	NN	denotes	activity
T15254	2003-2005	VB	denotes	is
T15255	2006-2014	VB	denotes	required
T15256	2015-2017	TO	denotes	to
T15257	2018-2023	VB	denotes	block
T15258	2024-2028	NN	denotes	RPS3
T15259	2029-2033	NN	denotes	S209
T15260	2034-2049	NN	denotes	phosphorylation
T15261	2051-2053	PRP	denotes	We
T15262	2054-2058	RB	denotes	next
T15263	2059-2067	VB	denotes	examined
T15264	2068-2075	IN	denotes	whether
T15265	2076-2079	DT	denotes	the
T15266	2080-2090	JJ	denotes	inhibitory
T15267	2091-2099	NN	denotes	activity
T15268	2100-2102	IN	denotes	of
T15269	2103-2108	NN	denotes	NleH1
T15270	2109-2111	VB	denotes	is
T15271	2112-2124	RB	denotes	sufficiently
T15272	2125-2131	JJ	denotes	robust
T15273	2132-2134	TO	denotes	to
T15274	2135-2141	VB	denotes	impair
T15275	2142-2145	DT	denotes	the
T15276	2146-2152	JJ	denotes	strong
T15277	2153-2160	JJ	denotes	nuclear
T15278	2161-2174	NN	denotes	translocation
T15279	2175-2177	IN	denotes	of
T15280	2178-2182	NN	denotes	RPS3
T15281	2183-2190	VB	denotes	trigged
T15282	2191-2193	IN	denotes	by
T15283	2194-2197	DT	denotes	the
T15284	2198-2219	JJ	denotes	constitutively-active
T15285	2220-2224	NN	denotes	IKKβ
T15286	2225-2226	-LRB-	denotes	(
T15287	2226-2230	NN	denotes	IKKβ
T15288	2231-2232	-LRB-	denotes	[
T15289	2232-2236	NN	denotes	SSEE
T15290	2236-2237	-RRB-	denotes	]
T15291	2237-2238	-RRB-	denotes	)
T15292	2239-2240	-LRB-	denotes	(
T15293	2240-2244	NNP	denotes	Fig.
T15294	2245-2247	NNP	denotes	2d
T15295	2247-2248	-RRB-	denotes	)
T15296	2250-2252	PRP	denotes	We
T15297	2253-2258	VB	denotes	found
T15298	2259-2263	IN	denotes	that
T15299	2264-2275	RB	denotes	ectopically
T15300	2276-2286	VB	denotes	expressing
T15301	2287-2293	CC	denotes	either
T15302	2294-2303	JJ	denotes	wild-type
T15303	2304-2306	CC	denotes	or
T15304	2307-2311	NN	denotes	SSEE
T15305	2312-2316	NN	denotes	IKKβ
T15306	2317-2325	NN	denotes	proteins
T15307	2325-2326	-COMMA-	denotes	,
T15308	2327-2336	VB	denotes	triggered
T15309	2337-2341	JJ	denotes	more
T15310	2342-2346	NN	denotes	RPS3
T15311	2347-2354	JJ	denotes	nuclear
T15312	2355-2368	NN	denotes	translocation
T15313	2369-2373	IN	denotes	than
T15314	2374-2377	DT	denotes	the
T15315	2378-2389	JJ	denotes	kinase-dead
T15316	2390-2394	NN	denotes	IKKβ
T15317	2395-2396	-LRB-	denotes	(
T15318	2396-2400	NN	denotes	SSAA
T15319	2400-2401	-RRB-	denotes	)
T15320	2402-2409	NN	denotes	protein
T15321	2410-2411	-LRB-	denotes	(
T15322	2411-2415	NNP	denotes	Fig.
T15323	2416-2418	NNP	denotes	7d
T15324	2418-2419	-RRB-	denotes	)
T15325	2421-2425	NN	denotes	RPS3
T15326	2426-2433	JJ	denotes	nuclear
T15327	2434-2446	NN	denotes	accumulation
T15328	2447-2450	VB	denotes	was
T15329	2451-2464	RB	denotes	substantially
T15330	2465-2473	VB	denotes	retarded
T15331	2474-2476	IN	denotes	by
T15332	2477-2486	VB	denotes	infecting
T15333	2487-2492	NN	denotes	cells
T15334	2493-2497	IN	denotes	with
T15335	2498-2507	JJ	denotes	wild-type
T15336	2508-2510	FW	denotes	E.
T15337	2511-2515	FW	denotes	coli
T15338	2516-2520	NN	denotes	O157
T15339	2520-2521	-COLON-	denotes	:
T15340	2521-2523	NN	denotes	H7
T15341	2524-2525	-LRB-	denotes	(
T15342	2525-2529	NNP	denotes	Fig.
T15343	2530-2532	NNP	denotes	7d
T15344	2532-2533	-RRB-	denotes	)
T15345	2535-2537	IN	denotes	In
T15346	2538-2546	NN	denotes	contrast
T15347	2546-2547	-COMMA-	denotes	,
T15348	2548-2557	VB	denotes	infecting
T15349	2558-2562	IN	denotes	with
T15350	2563-2569	CC	denotes	either
T15351	2570-2576	NN	denotes	ΔnleH1
T15352	2577-2579	CC	denotes	or
T15353	2580-2585	NN	denotes	ΔescN
T15354	2586-2593	NN	denotes	strains
T15355	2594-2598	RB	denotes	only
T15356	2599-2607	RB	denotes	slightly
T15357	2608-2616	JJ	denotes	impaired
T15358	2617-2621	NN	denotes	RPS3
T15359	2622-2629	JJ	denotes	nuclear
T15360	2630-2643	NN	denotes	translocation
T15361	2644-2646	IN	denotes	in
T15362	2647-2653	CC	denotes	either
T15363	2654-2659	NN	denotes	IKKβ-
T15364	2660-2662	CC	denotes	or
T15365	2663-2667	NN	denotes	IKKβ
T15366	2668-2669	-LRB-	denotes	(
T15367	2669-2673	NN	denotes	SSEE
T15368	2673-2674	-RRB-	denotes	)
T15369	2674-2685	JJ	denotes	-expressing
T15370	2686-2691	NN	denotes	cells
T15371	2692-2693	-LRB-	denotes	(
T15372	2693-2697	NNP	denotes	Fig.
T15373	2698-2700	NNP	denotes	7d
T15374	2700-2701	-RRB-	denotes	)
T15375	2703-2705	IN	denotes	As
T15376	2706-2714	VB	denotes	expected
T15377	2714-2715	-COMMA-	denotes	,
T15378	2716-2718	NNP	denotes	E.
T15379	2719-2723	NNP	denotes	coli
T15380	2724-2734	NN	denotes	infections
T15381	2735-2738	VB	denotes	did
T15382	2739-2742	RB	denotes	not
T15383	2743-2749	VB	denotes	affect
T15384	2750-2753	DT	denotes	the
T15385	2754-2758	NN	denotes	RPS3
T15386	2759-2766	JJ	denotes	nuclear
T15387	2767-2780	NN	denotes	translocation
T15388	2781-2783	IN	denotes	in
T15389	2784-2788	NN	denotes	IKKβ
T15390	2789-2790	-LRB-	denotes	(
T15391	2790-2794	NN	denotes	SSAA
T15392	2794-2795	-RRB-	denotes	)
T15393	2795-2806	JJ	denotes	-expressing
T15394	2807-2812	NN	denotes	cells
T15395	2812-2813	-COMMA-	denotes	,
T15396	2814-2819	WRB	denotes	where
T15397	2820-2825	NN	denotes	NF-κB
T15398	2826-2835	NN	denotes	signaling
T15399	2836-2839	VB	denotes	was
T15400	2840-2843	JJ	denotes	low
T15401	2844-2845	-LRB-	denotes	(
T15402	2845-2849	NNP	denotes	Fig.
T15403	2850-2852	NNP	denotes	7d
T15404	2852-2853	-RRB-	denotes	)
T15405	2855-2859	RB	denotes	Thus
T15406	2859-2860	-COMMA-	denotes	,
T15407	2861-2867	IN	denotes	during
T15408	2868-2877	NN	denotes	infection
T15409	2878-2883	NN	denotes	NleH1
T15410	2884-2886	VB	denotes	is
T15411	2887-2899	RB	denotes	sufficiently
T15412	2900-2906	JJ	denotes	potent
T15413	2907-2909	TO	denotes	to
T15414	2910-2917	VB	denotes	inhibit
T15415	2918-2922	NN	denotes	RPS3
T15416	2923-2930	JJ	denotes	nuclear
T15417	2931-2944	NN	denotes	translocation
T15418	2945-2949	RB	denotes	even
T15419	2950-2952	IN	denotes	in
T15420	2953-2958	NN	denotes	cells
T15421	2959-2969	VB	denotes	expressing
T15422	2970-2994	JJ	denotes	constitutively-activated
T15423	2995-2999	NN	denotes	IKKβ
T15424	3001-3003	PRP	denotes	We
T15425	3004-3012	VB	denotes	examined
T15426	3013-3020	IN	denotes	whether
T15427	3021-3026	NN	denotes	NleH1
T15428	3027-3032	MD	denotes	could
T15429	3033-3041	RB	denotes	directly
T15430	3042-3055	VB	denotes	phosphorylate
T15431	3056-3060	NN	denotes	IKKβ
T15432	3061-3065	RB	denotes	thus
T15433	3066-3076	VB	denotes	inhibiting
T15434	3077-3090	JJ	denotes	IKKβ-mediated
T15435	3091-3095	NN	denotes	RPS3
T15436	3096-3100	NN	denotes	S209
T15437	3101-3116	NN	denotes	phosphorylation
T15438	3118-3120	PRP	denotes	We
T15439	3121-3130	VB	denotes	performed
T15440	3131-3133	FW	denotes	in
T15441	3134-3139	FW	denotes	vitro
T15442	3140-3146	NN	denotes	kinase
T15443	3147-3153	NN	denotes	assays
T15444	3154-3159	VB	denotes	using
T15445	3160-3178	VB	denotes	immunoprecipitated
T15446	3179-3188	NN	denotes	Flag-IKKβ
T15447	3189-3190	-LRB-	denotes	(
T15448	3190-3194	NN	denotes	K44A
T15449	3194-3195	-RRB-	denotes	)
T15450	3196-3198	IN	denotes	as
T15451	3199-3208	NN	denotes	substrate
T15452	3209-3212	CC	denotes	and
T15453	3213-3224	JJ	denotes	recombinant
T15454	3225-3234	NN	denotes	His-NleH1
T15455	3235-3237	IN	denotes	as
T15456	3238-3244	NN	denotes	kinase
T15457	3244-3245	-COMMA-	denotes	,
T15458	3246-3248	IN	denotes	so
T15459	3249-3253	IN	denotes	that
T15460	3254-3258	NN	denotes	IKKβ
T15461	3259-3278	NN	denotes	autophosphorylation
T15462	3279-3284	MD	denotes	would
T15463	3285-3288	RB	denotes	not
T15464	3289-3296	VB	denotes	obscure
T15465	3297-3310	JJ	denotes	NleH1-induced
T15466	3311-3326	NN	denotes	phosphorylation
T15467	3328-3335	RB	denotes	However
T15468	3335-3336	-COMMA-	denotes	,
T15469	3337-3339	PRP	denotes	we
T15470	3340-3343	VB	denotes	did
T15471	3344-3347	RB	denotes	not
T15472	3348-3355	VB	denotes	observe
T15473	3356-3359	DT	denotes	any
T15474	3360-3370	JJ	denotes	detectable
T15475	3371-3374	NN	denotes	32P
T15476	3375-3388	NN	denotes	incorporation
T15477	3389-3391	IN	denotes	in
T15478	3392-3396	NN	denotes	IKKβ
T15479	3397-3398	-LRB-	denotes	(
T15480	3398-3411	NNP	denotes	Supplementary
T15481	3412-3416	NNP	denotes	Fig.
T15482	3417-3419	CD	denotes	13
T15483	3419-3420	-RRB-	denotes	)
T15484	3420-3421	-COMMA-	denotes	,
T15485	3422-3426	RB	denotes	thus
T15486	3427-3433	VB	denotes	ruling
T15487	3434-3437	RP	denotes	out
T15488	3438-3442	DT	denotes	this
T15489	3443-3454	NN	denotes	possibility
T15490	3456-3458	PRP	denotes	We
T15491	3459-3463	RB	denotes	then
T15492	3464-3470	VB	denotes	tested
T15493	3471-3474	DT	denotes	the
T15494	3475-3485	NN	denotes	hypothesis
T15495	3486-3490	IN	denotes	that
T15496	3491-3496	NN	denotes	NleH1
T15497	3497-3502	MD	denotes	could
T15498	3503-3508	VB	denotes	alter
T15499	3509-3512	DT	denotes	the
T15500	3513-3517	NN	denotes	IKKβ
T15501	3518-3527	NN	denotes	substrate
T15502	3528-3541	NN	denotes	specificities
T15503	3543-3545	TO	denotes	To
T15504	3546-3550	DT	denotes	this
T15505	3551-3554	NN	denotes	end
T15506	3554-3555	-COMMA-	denotes	,
T15507	3556-3558	PRP	denotes	we
T15508	3559-3568	VB	denotes	performed
T15509	3569-3571	FW	denotes	in
T15510	3572-3577	FW	denotes	vitro
T15511	3578-3584	NN	denotes	kinase
T15512	3585-3591	NN	denotes	assays
T15513	3592-3597	VB	denotes	using
T15514	3598-3602	CC	denotes	both
T15515	3603-3606	NN	denotes	CK2
T15516	3607-3610	CC	denotes	and
T15517	3611-3614	NN	denotes	IKK
T15518	3615-3625	NN	denotes	substrates
T15519	3626-3629	IN	denotes	for
T15520	3630-3634	NN	denotes	IKKβ
T15521	3636-3638	IN	denotes	As
T15522	3639-3647	VB	denotes	expected
T15523	3647-3648	-COMMA-	denotes	,
T15524	3649-3653	NN	denotes	IKKβ
T15525	3654-3668	VB	denotes	phosphorylated
T15526	3669-3673	NN	denotes	RPS3
T15527	3674-3675	-LRB-	denotes	(
T15528	3675-3679	NNP	denotes	Fig.
T15529	3680-3682	NNP	denotes	7e
T15530	3682-3683	-COMMA-	denotes	,
T15531	3684-3688	NN	denotes	lane
T15532	3689-3690	CD	denotes	7
T15533	3690-3691	-RRB-	denotes	)
T15534	3692-3695	CC	denotes	and
T15535	3696-3704	NN	denotes	GST-IκBα
T15536	3705-3706	-LRB-	denotes	(
T15537	3706-3710	CD	denotes	1-54
T15538	3710-3711	-RRB-	denotes	)
T15539	3712-3719	NN	denotes	protein
T15540	3720-3721	-LRB-	denotes	(
T15541	3721-3725	NNP	denotes	Fig.
T15542	3726-3728	NNP	denotes	7e
T15543	3728-3729	-COMMA-	denotes	,
T15544	3730-3734	NN	denotes	lane
T15545	3735-3736	CD	denotes	9
T15546	3736-3737	-RRB-	denotes	)
T15547	3737-3738	-COMMA-	denotes	,
T15548	3739-3752	VB	denotes	demonstrating
T15549	3753-3755	PRP	denotes	it
T15550	3756-3763	VB	denotes	harbors
T15551	3764-3770	CC	denotes	either
T15552	3771-3774	NN	denotes	CK2
T15553	3775-3777	CC	denotes	or
T15554	3778-3781	NN	denotes	IKK
T15555	3782-3791	NN	denotes	substrate
T15556	3792-3803	NN	denotes	specificity
T15557	3805-3818	NN	denotes	Preincubation
T15558	3819-3821	IN	denotes	of
T15559	3822-3826	NN	denotes	IKKβ
T15560	3827-3831	IN	denotes	with
T15561	3832-3837	NN	denotes	NleH1
T15562	3838-3845	VB	denotes	reduced
T15563	3846-3859	JJ	denotes	IKKβ-mediated
T15564	3860-3864	NN	denotes	RPS3
T15565	3865-3880	NN	denotes	phosphorylation
T15566	3880-3881	-COMMA-	denotes	,
T15567	3882-3886	FW	denotes	i.e.
T15568	3887-3890	DT	denotes	the
T15569	3891-3894	NN	denotes	CK2
T15570	3895-3901	NN	denotes	kinase
T15571	3902-3913	NN	denotes	specificity
T15572	3913-3914	-COMMA-	denotes	,
T15573	3915-3918	CC	denotes	but
T15574	3919-3922	RB	denotes	not
T15575	3923-3936	JJ	denotes	IKKβ-mediated
T15576	3937-3945	NN	denotes	GST-IκBα
T15577	3946-3961	NN	denotes	phosphorylation
T15578	3961-3962	-COMMA-	denotes	,
T15579	3963-3967	FW	denotes	i.e.
T15580	3968-3971	DT	denotes	the
T15581	3972-3975	NN	denotes	IKK
T15582	3976-3982	NN	denotes	kinase
T15583	3983-3994	NN	denotes	specificity
T15584	3995-3996	-LRB-	denotes	(
T15585	3996-4000	NNP	denotes	Fig.
T15586	4001-4003	NNP	denotes	7e
T15587	4003-4004	-RRB-	denotes	)
T15588	4006-4013	NN	denotes	Control
T15589	4014-4025	NN	denotes	experiments
T15590	4026-4034	VB	denotes	revealed
T15591	4035-4037	DT	denotes	no
T15592	4038-4052	JJ	denotes	NleH1-mediated
T15593	4053-4068	NN	denotes	phosphorylation
T15594	4069-4071	CC	denotes	or
T15595	4072-4091	NN	denotes	autophosphorylation
T15596	4092-4094	IN	denotes	of
T15597	4095-4099	NN	denotes	RPS3
T15598	4100-4102	CC	denotes	or
T15599	4103-4111	NN	denotes	GST-IκBα
T15600	4112-4113	-LRB-	denotes	(
T15601	4113-4117	NNP	denotes	Fig.
T15602	4118-4120	NNP	denotes	7e
T15603	4120-4121	-RRB-	denotes	)
T15604	4123-4128	VB	denotes	Taken
T15605	4129-4137	RB	denotes	together
T15606	4137-4138	-COMMA-	denotes	,
T15607	4139-4144	NN	denotes	NleH1
T15608	4145-4151	VB	denotes	blocks
T15609	4152-4155	DT	denotes	the
T15610	4156-4159	NN	denotes	CK2
T15611	4160-4169	NN	denotes	substrate
T15612	4170-4181	NN	denotes	specificity
T15613	4182-4184	IN	denotes	of
T15614	4185-4189	NN	denotes	IKKβ
T15615	4190-4194	RB	denotes	thus
T15616	4195-4205	VB	denotes	inhibiting
T15617	4206-4209	DT	denotes	the
T15618	4210-4223	JJ	denotes	IKKβ-mediated
T15619	4224-4228	NN	denotes	RPS3
T15620	4229-4233	NN	denotes	S209
T15621	4234-4249	NN	denotes	phosphorylation
T15622	4250-4254	RB	denotes	thus
T15623	4255-4267	VB	denotes	representing
T15624	4268-4269	DT	denotes	a
T15625	4270-4275	JJ	denotes	novel
T15626	4276-4284	NN	denotes	strategy
T15627	4285-4287	IN	denotes	by
T15628	4288-4290	FW	denotes	E.
T15629	4291-4295	FW	denotes	coli
T15630	4296-4300	NN	denotes	O157
T15631	4300-4301	-COLON-	denotes	:
T15632	4301-4303	NN	denotes	H7
T15633	4304-4306	TO	denotes	to
T15634	4307-4312	VB	denotes	alter
T15635	4313-4316	DT	denotes	the
T15636	4317-4321	NN	denotes	host
T15637	4322-4328	JJ	denotes	innate
T15638	4329-4335	JJ	denotes	immune
T15639	4336-4344	NN	denotes	response
R11926	T14935	T14934	arg1Of	steers,NleH1
R11927	T14935	T14937	arg1Of	steers,IKKβ
R11928	T14937	T14936	arg1Of	IKKβ,the
R11929	T14939	T14935	arg1Of	specificities,steers
R11930	T14939	T14938	arg1Of	specificities,substrate
R11931	T14940	T14941	arg1Of	NleH1,is
R11932	T14945	T14941	arg2Of	kinase,is
R11933	T14945	T14942	arg1Of	kinase,an
R11934	T14945	T14943	arg2Of	kinase,autophosphorylated
R11935	T14945	T14944	arg1Of	kinase,serine-threonine
R11936	T14945	T14946	arg1Of	kinase,","
R11937	T14945	T14947	arg1Of	kinase,which
R11938	T14945	T14948	arg1Of	kinase,depends
R11939	T14948	T14949	arg1Of	depends,on
R11940	T14951	T14949	arg2Of	lysine,on
R11941	T14951	T14950	arg1Of	lysine,the
R11942	T14951	T14952	arg1Of	lysine,159
R11943	T14951	T14953	arg1Of	lysine,(
R11944	T14951	T14956	arg1Of	lysine,9
R11945	T14954	T14953	arg2Of	K159,(
R11946	T14955	T14953	arg3Of	),(
R11947	T14958	T14957	arg1Of	explore,To
R11948	T14960	T14958	arg2Of	mechanism,explore
R11949	T14960	T14959	arg1Of	mechanism,the
R11950	T14960	T14961	arg2Of	mechanism,by
R11951	T14960	T14962	arg1Of	mechanism,which
R11952	T14963	T14964	arg1Of	NleH1,inhibits
R11953	T14964	T14961	arg1Of	inhibits,by
R11954	T14967	T14964	arg2Of	phosphorylation,inhibits
R11955	T14967	T14965	arg1Of	phosphorylation,RPS3
R11956	T14967	T14966	arg1Of	phosphorylation,S209
R11957	T14969	T14958	arg1Of	we,explore
R11958	T14969	T14971	arg1Of	we,performed
R11959	T14971	T14957	modOf	performed,To
R11960	T14971	T14968	arg1Of	performed,","
R11961	T14971	T14970	arg1Of	performed,first
R11962	T14971	T14990	arg1Of	performed,","
R11963	T14971	T14991	modOf	performed,confirming
R11964	T14974	T14973	arg1Of	vitro,in
R11965	T14976	T14971	arg2Of	assay,performed
R11966	T14976	T14972	arg1Of	assay,an
R11967	T14976	T14974	arg1Of	assay,vitro
R11968	T14976	T14975	arg1Of	assay,kinase
R11969	T14976	T14977	arg1Of	assay,with
R11970	T14981	T14978	arg2Of	protein,purified
R11971	T14981	T14979	arg1Of	protein,wild-type
R11972	T14981	T14980	arg1Of	protein,His-NleH1
R11973	T14981	T14982	arg1Of	protein,and
R11974	T14982	T14977	arg2Of	and,with
R11975	T14987	T14986	arg2Of	K159A,(
R11976	T14988	T14986	arg3Of	),(
R11977	T14989	T14982	arg2Of	protein,and
R11978	T14989	T14983	arg1Of	protein,a
R11979	T14989	T14984	arg1Of	protein,mutant
R11980	T14989	T14985	arg1Of	protein,His-NleH1
R11981	T14989	T14986	arg1Of	protein,(
R11982	T14993	T14994	arg1Of	NleH1,is
R11983	T14993	T14995	arg2Of	NleH1,autophosphorylated
R11984	T14995	T14994	arg2Of	autophosphorylated,is
R11985	T14995	T14996	arg1Of	autophosphorylated,and
R11986	T14996	T14991	arg2Of	and,confirming
R11987	T14996	T14992	arg1Of	and,that
R11988	T14998	T14997	arg1Of	K159A,the
R11989	T14998	T14999	arg1Of	K159A,is
R11990	T14999	T14996	arg2Of	is,and
R11991	T15003	T14999	arg2Of	mutant,is
R11992	T15003	T15000	arg1Of	mutant,an
R11993	T15003	T15001	arg1Of	mutant,NleH1
R11994	T15003	T15002	arg1Of	mutant,kinase-dead
R11995	T15003	T15004	arg1Of	mutant,(
R11996	T15006	T15004	arg2Of	7a,(
R11997	T15006	T15005	arg1Of	7a,Fig.
R11998	T15007	T15004	arg3Of	),(
R11999	T15009	T15008	arg1Of	examine,To
R12000	T15013	T15011	arg1Of	activity,the
R12001	T15013	T15012	arg1Of	activity,kinase
R12002	T15013	T15014	arg1Of	activity,is
R12003	T15013	T15015	arg2Of	activity,required
R12004	T15015	T15009	arg2Of	required,examine
R12005	T15015	T15010	arg1Of	required,whether
R12006	T15015	T15014	arg2Of	required,is
R12007	T15015	T15016	arg1Of	required,for
R12008	T15015	T15018	modOf	required,to
R12009	T15017	T15016	arg2Of	NleH1,for
R12010	T15019	T15018	arg1Of	inhibit,to
R12011	T15021	T15019	arg2Of	phosphorlyation,inhibit
R12012	T15021	T15020	arg1Of	phosphorlyation,IKKβ
R12013	T15021	T15022	arg1Of	phosphorlyation,of
R12014	T15021	T15024	arg1Of	phosphorlyation,on
R12015	T15023	T15022	arg2Of	RPS3,of
R12016	T15025	T15024	arg2Of	S209,on
R12017	T15027	T15029	arg1Of	we,expressing
R12018	T15029	T15008	modOf	expressing,To
R12019	T15029	T15026	arg1Of	expressing,","
R12020	T15029	T15028	arg1Of	expressing,ectopically
R12021	T15031	T15032	arg1Of	wild-type,or
R12022	T15032	T15030	arg1Of	or,either
R12023	T15033	T15032	arg2Of	K159A,or
R12024	T15034	T15029	arg2Of	NleH1,expressing
R12025	T15034	T15031	arg1Of	NleH1,wild-type
R12026	T15034	T15033	arg1Of	NleH1,K159A
R12027	T15034	T15035	arg1Of	NleH1,in
R12028	T15037	T15035	arg2Of	cells,in
R12029	T15037	T15036	arg1Of	cells,293T
R12030	T15040	T15038	arg1Of	expression,Wild-type
R12031	T15040	T15039	arg1Of	expression,NleH1
R12032	T15040	T15042	arg1Of	expression,reduced
R12033	T15042	T15041	arg1Of	reduced,significantly
R12034	T15042	T15047	arg1Of	reduced,","
R12035	T15042	T15048	arg1Of	reduced,whereas
R12036	T15046	T15042	arg2Of	phosphorylation,reduced
R12037	T15046	T15043	arg1Of	phosphorylation,TNF-induced
R12038	T15046	T15044	arg1Of	phosphorylation,RPS3
R12039	T15046	T15045	arg1Of	phosphorylation,S209
R12040	T15051	T15049	arg1Of	mutant,the
R12041	T15051	T15050	arg1Of	mutant,K159A
R12042	T15051	T15052	arg1Of	mutant,failed
R12043	T15051	T15054	arg1Of	mutant,do
R12044	T15051	T15055	arg1Of	mutant,so
R12045	T15052	T15048	arg2Of	failed,whereas
R12046	T15052	T15056	arg1Of	failed,(
R12047	T15054	T15052	arg2Of	do,failed
R12048	T15054	T15053	arg1Of	do,to
R12049	T15055	T15054	arg2Of	so,do
R12050	T15058	T15056	arg2Of	7b,(
R12051	T15058	T15057	arg1Of	7b,Fig.
R12052	T15059	T15056	arg3Of	),(
R12053	T15063	T15061	arg1Of	activity,NleH1
R12054	T15063	T15062	arg1Of	activity,kinase
R12055	T15063	T15064	arg1Of	activity,is
R12056	T15063	T15065	arg2Of	activity,required
R12057	T15065	T15060	arg1Of	required,Thus
R12058	T15065	T15064	arg2Of	required,is
R12059	T15065	T15066	modOf	required,to
R12060	T15067	T15066	arg1Of	protect,to
R12061	T15067	T15069	arg1Of	protect,from
R12062	T15068	T15067	arg2Of	RPS3,protect
R12063	T15071	T15069	arg2Of	phosphorylation,from
R12064	T15071	T15070	arg1Of	phosphorylation,IKKβ-mediated
R12065	T15073	T15072	arg1Of	rodentium,Citrobacter
R12066	T15073	T15074	arg1Of	rodentium,is
R12067	T15074	T15092	arg1Of	is,","
R12068	T15077	T15074	arg2Of	pathogen,is
R12069	T15077	T15075	arg1Of	pathogen,a
R12070	T15077	T15076	arg1Of	pathogen,mouse
R12071	T15077	T15078	arg1Of	pathogen,that
R12072	T15077	T15079	arg1Of	pathogen,shares
R12073	T15079	T15086	arg1Of	shares,","
R12074	T15079	T15089	arg1Of	shares,for
R12075	T15081	T15079	arg2Of	strategies,shares
R12076	T15081	T15080	arg1Of	strategies,pathogenic
R12077	T15081	T15082	arg1Of	strategies,with
R12078	T15084	T15082	arg2Of	coli,with
R12079	T15084	T15083	arg1Of	coli,E.
R12080	T15084	T15085	arg1Of	coli,39
R12081	T15088	T15087	arg1Of	notably,most
R12082	T15089	T15088	arg1Of	for,notably
R12083	T15091	T15089	arg2Of	investigation,for
R12084	T15091	T15090	arg1Of	investigation,our
R12085	T15095	T15093	arg1Of	NleH,C.
R12086	T15095	T15094	arg1Of	NleH,rodentium
R12087	T15095	T15096	arg1Of	NleH,inhibited
R12088	T15096	T15092	arg2Of	inhibited,","
R12089	T15099	T15097	arg1Of	translocation,RPS3
R12090	T15099	T15098	arg1Of	translocation,nuclear
R12091	T15099	T15100	arg1Of	translocation,and
R12092	T15100	T15096	arg2Of	and,inhibited
R12093	T15100	T15105	arg1Of	and,to
R12094	T15104	T15100	arg2Of	activity,and
R12095	T15104	T15101	arg1Of	activity,RPS3-dependent
R12096	T15104	T15102	arg1Of	activity,NF-κB
R12097	T15104	T15103	arg1Of	activity,luciferase
R12098	T15107	T15105	arg2Of	extent,to
R12099	T15107	T15106	arg1Of	extent,an
R12100	T15107	T15108	arg1Of	extent,equivalent
R12101	T15108	T15109	arg1Of	equivalent,to
R12102	T15112	T15109	arg2Of	NleH1,to
R12103	T15112	T15110	arg1Of	NleH1,E.
R12104	T15112	T15111	arg1Of	NleH1,coli
R12105	T15114	T15096	arg3Of	ref,inhibited
R12106	T15114	T15113	arg1Of	ref,(
R12107	T15115	T15116	arg1Of	9,)
R12108	T15117	T15118	arg1Of	We,assayed
R12109	T15118	T15122	arg1Of	assayed,in
R12110	T15121	T15118	arg2Of	phosphorylation,assayed
R12111	T15121	T15119	arg1Of	phosphorylation,RPS3
R12112	T15121	T15120	arg1Of	phosphorylation,S209
R12113	T15124	T15122	arg2Of	cells,in
R12114	T15124	T15123	arg1Of	cells,HeLa
R12115	T15124	T15125	arg2Of	cells,infected
R12116	T15125	T15126	arg1Of	infected,with
R12117	T15130	T15126	arg2Of	strains,with
R12118	T15130	T15127	arg1Of	strains,different
R12119	T15130	T15128	arg1Of	strains,C.
R12120	T15130	T15129	arg1Of	strains,rodentium
R12121	T15133	T15131	arg2Of	cells,In
R12122	T15133	T15132	arg1Of	cells,uninfected
R12123	T15135	T15136	arg1Of	TNF-treatment,stimulated
R12124	T15136	T15131	arg1Of	stimulated,In
R12125	T15136	T15134	arg1Of	stimulated,","
R12126	T15139	T15136	arg2Of	increase,stimulated
R12127	T15139	T15137	arg1Of	increase,a
R12128	T15139	T15138	arg1Of	increase,∼3.5-fold
R12129	T15139	T15140	arg1Of	increase,in
R12130	T15143	T15140	arg2Of	phosphorylation,in
R12131	T15143	T15141	arg1Of	phosphorylation,RPS3
R12132	T15143	T15142	arg1Of	phosphorylation,S209
R12133	T15143	T15144	arg1Of	phosphorylation,(
R12134	T15146	T15144	arg2Of	7c,(
R12135	T15146	T15145	arg1Of	7c,Fig.
R12136	T15147	T15144	arg3Of	),(
R12137	T15149	T15148	arg1Of	augmentation,Such
R12138	T15149	T15150	arg1Of	augmentation,of
R12139	T15149	T15153	arg1Of	augmentation,was
R12140	T15149	T15154	arg2Of	augmentation,reduced
R12141	T15152	T15150	arg2Of	phosphorylation,of
R12142	T15152	T15151	arg1Of	phosphorylation,RPS3
R12143	T15154	T15153	arg2Of	reduced,was
R12144	T15154	T15155	arg1Of	reduced,by
R12145	T15157	T15156	arg1Of	60,about
R12146	T15158	T15155	arg2Of	%,by
R12147	T15158	T15157	arg1Of	%,60
R12148	T15163	T15154	arg1Of	infection,reduced
R12149	T15163	T15159	arg2Of	infection,by
R12150	T15163	T15160	arg1Of	infection,wild-type
R12151	T15163	T15161	arg1Of	infection,C.
R12152	T15163	T15162	arg1Of	infection,rodentium
R12153	T15163	T15164	arg1Of	infection,(
R12154	T15166	T15164	arg2Of	7c,(
R12155	T15166	T15165	arg1Of	7c,Fig.
R12156	T15167	T15164	arg3Of	),(
R12157	T15171	T15170	arg1Of	phosphorylation,RPS3
R12158	T15171	T15172	arg1Of	phosphorylation,was
R12159	T15171	T15173	arg2Of	phosphorylation,enhanced
R12160	T15173	T15168	arg1Of	enhanced,However
R12161	T15173	T15169	arg1Of	enhanced,","
R12162	T15173	T15172	arg2Of	enhanced,was
R12163	T15173	T15174	arg1Of	enhanced,when
R12164	T15175	T15176	arg1Of	cells,were
R12165	T15175	T15177	arg2Of	cells,infected
R12166	T15177	T15174	arg2Of	infected,when
R12167	T15177	T15176	arg2Of	infected,were
R12168	T15177	T15178	arg1Of	infected,with
R12169	T15182	T15178	arg2Of	strain,with
R12170	T15182	T15179	arg1Of	strain,a
R12171	T15182	T15180	arg1Of	strain,C.
R12172	T15182	T15181	arg1Of	strain,rodentium
R12173	T15182	T15183	arg1Of	strain,lacking
R12174	T15184	T15183	arg2Of	NleH,lacking
R12175	T15184	T15185	arg1Of	NleH,(
R12176	T15187	T15185	arg2Of	7c,(
R12177	T15187	T15186	arg1Of	7c,Fig.
R12178	T15187	T15188	arg1Of	7c,","
R12179	T15189	T15188	arg2Of	ΔnleH,","
R12180	T15190	T15185	arg3Of	),(
R12181	T15191	T15193	arg1Of	We,examined
R12182	T15193	T15192	arg1Of	examined,further
R12183	T15195	T15193	arg2Of	role,examined
R12184	T15195	T15194	arg1Of	role,the
R12185	T15195	T15196	arg1Of	role,of
R12186	T15195	T15200	arg1Of	role,using
R12187	T15199	T15196	arg2Of	activity,of
R12188	T15199	T15197	arg1Of	activity,NleH1
R12189	T15199	T15198	arg1Of	activity,kinase
R12190	T15200	T15206	arg1Of	using,as
R12191	T15205	T15200	arg2Of	strain,using
R12192	T15205	T15201	arg1Of	strain,the
R12193	T15205	T15202	arg1Of	strain,C.
R12194	T15205	T15203	arg1Of	strain,rodentium
R12195	T15205	T15204	arg1Of	strain,ΔnleH
R12196	T15208	T15206	arg2Of	background,as
R12197	T15208	T15207	arg1Of	background,a
R12198	T15208	T15209	arg1Of	background,on
R12199	T15210	T15209	arg2Of	which,on
R12200	T15210	T15213	arg2Of	which,either
R12201	T15212	T15211	arg1Of	express,to
R12202	T15212	T15213	arg1Of	express,either
R12203	T15214	T15215	arg1Of	wild-type,or
R12204	T15215	T15212	arg2Of	or,express
R12205	T15219	T15215	arg2Of	NleH1,or
R12206	T15219	T15216	arg1Of	NleH1,K159A
R12207	T15219	T15217	arg1Of	NleH1,E.
R12208	T15219	T15218	arg1Of	NleH1,coli
R12209	T15222	T15220	arg1Of	mutant,Complementing
R12210	T15222	T15221	arg1Of	mutant,ΔnleH
R12211	T15222	T15223	arg1Of	mutant,with
R12212	T15222	T15227	arg1Of	mutant,abolished
R12213	T15225	T15223	arg2Of	NleH1,with
R12214	T15225	T15224	arg1Of	NleH1,wild-type
R12215	T15227	T15226	arg1Of	abolished,almost
R12216	T15227	T15232	arg1Of	abolished,whereas
R12217	T15231	T15227	arg2Of	phosphorylation,abolished
R12218	T15231	T15228	arg1Of	phosphorylation,TNF-induced
R12219	T15231	T15229	arg1Of	phosphorylation,RPS3
R12220	T15231	T15230	arg1Of	phosphorylation,S209
R12221	T15233	T15234	arg1Of	complementing,with
R12222	T15233	T15236	arg1Of	complementing,failed
R12223	T15233	T15238	arg1Of	complementing,inhibit
R12224	T15235	T15234	arg2Of	K159A,with
R12225	T15236	T15232	arg2Of	failed,whereas
R12226	T15238	T15236	arg2Of	inhibit,failed
R12227	T15238	T15237	arg1Of	inhibit,to
R12228	T15240	T15238	arg2Of	phosphorylation,inhibit
R12229	T15240	T15239	arg1Of	phosphorylation,RPS3
R12230	T15240	T15241	arg1Of	phosphorylation,(
R12231	T15243	T15241	arg2Of	7c,(
R12232	T15243	T15242	arg1Of	7c,Fig.
R12233	T15244	T15241	arg3Of	),(
R12234	T15248	T15247	arg1Of	results,these
R12235	T15248	T15249	arg1Of	results,demonstrate
R12236	T15249	T15245	arg1Of	demonstrate,Collectively
R12237	T15249	T15246	arg1Of	demonstrate,","
R12238	T15253	T15251	arg1Of	activity,NleH1
R12239	T15253	T15252	arg1Of	activity,kinase
R12240	T15253	T15254	arg1Of	activity,is
R12241	T15253	T15255	arg2Of	activity,required
R12242	T15253	T15257	arg1Of	activity,block
R12243	T15255	T15249	arg2Of	required,demonstrate
R12244	T15255	T15250	arg1Of	required,that
R12245	T15255	T15254	arg2Of	required,is
R12246	T15257	T15255	arg3Of	block,required
R12247	T15257	T15256	arg1Of	block,to
R12248	T15260	T15257	arg2Of	phosphorylation,block
R12249	T15260	T15258	arg1Of	phosphorylation,RPS3
R12250	T15260	T15259	arg1Of	phosphorylation,S209
R12251	T15261	T15263	arg1Of	We,examined
R12252	T15263	T15262	arg1Of	examined,next
R12253	T15267	T15265	arg1Of	activity,the
R12254	T15267	T15266	arg1Of	activity,inhibitory
R12255	T15267	T15268	arg1Of	activity,of
R12256	T15267	T15270	arg1Of	activity,is
R12257	T15267	T15272	arg1Of	activity,robust
R12258	T15269	T15268	arg2Of	NleH1,of
R12259	T15270	T15263	arg2Of	is,examined
R12260	T15270	T15264	arg1Of	is,whether
R12261	T15272	T15270	arg2Of	robust,is
R12262	T15272	T15271	arg1Of	robust,sufficiently
R12263	T15272	T15273	modOf	robust,to
R12264	T15274	T15273	arg1Of	impair,to
R12265	T15278	T15274	arg2Of	translocation,impair
R12266	T15278	T15275	arg1Of	translocation,the
R12267	T15278	T15276	arg1Of	translocation,strong
R12268	T15278	T15277	arg1Of	translocation,nuclear
R12269	T15278	T15279	arg1Of	translocation,of
R12270	T15280	T15279	arg2Of	RPS3,of
R12271	T15280	T15281	arg2Of	RPS3,trigged
R12272	T15281	T15292	arg1Of	trigged,(
R12273	T15285	T15281	arg1Of	IKKβ,trigged
R12274	T15285	T15282	arg2Of	IKKβ,by
R12275	T15285	T15283	arg1Of	IKKβ,the
R12276	T15285	T15284	arg1Of	IKKβ,constitutively-active
R12277	T15285	T15286	arg1Of	IKKβ,(
R12278	T15287	T15286	arg2Of	IKKβ,(
R12279	T15287	T15288	arg1Of	IKKβ,[
R12280	T15289	T15288	arg2Of	SSEE,[
R12281	T15290	T15288	arg3Of	],[
R12282	T15291	T15286	arg3Of	),(
R12283	T15294	T15292	arg2Of	2d,(
R12284	T15294	T15293	arg1Of	2d,Fig.
R12285	T15295	T15292	arg3Of	),(
R12286	T15296	T15297	arg1Of	We,found
R12287	T15300	T15299	arg2Of	expressing,ectopically
R12288	T15300	T15301	arg1Of	expressing,either
R12289	T15302	T15303	arg1Of	wild-type,or
R12290	T15303	T15300	arg1Of	or,expressing
R12291	T15303	T15308	arg1Of	or,triggered
R12292	T15306	T15303	arg2Of	proteins,or
R12293	T15306	T15304	arg1Of	proteins,SSEE
R12294	T15306	T15305	arg1Of	proteins,IKKβ
R12295	T15308	T15297	arg2Of	triggered,found
R12296	T15308	T15298	arg1Of	triggered,that
R12297	T15308	T15299	arg1Of	triggered,ectopically
R12298	T15308	T15307	arg1Of	triggered,","
R12299	T15312	T15308	arg2Of	translocation,triggered
R12300	T15312	T15309	arg1Of	translocation,more
R12301	T15312	T15310	arg1Of	translocation,RPS3
R12302	T15312	T15311	arg1Of	translocation,nuclear
R12303	T15312	T15313	arg1Of	translocation,than
R12304	T15318	T15317	arg2Of	SSAA,(
R12305	T15319	T15317	arg3Of	),(
R12306	T15320	T15313	arg2Of	protein,than
R12307	T15320	T15314	arg1Of	protein,the
R12308	T15320	T15315	arg1Of	protein,kinase-dead
R12309	T15320	T15316	arg1Of	protein,IKKβ
R12310	T15320	T15317	arg1Of	protein,(
R12311	T15320	T15321	arg1Of	protein,(
R12312	T15323	T15321	arg2Of	7d,(
R12313	T15323	T15322	arg1Of	7d,Fig.
R12314	T15324	T15321	arg3Of	),(
R12315	T15327	T15325	arg1Of	accumulation,RPS3
R12316	T15327	T15326	arg1Of	accumulation,nuclear
R12317	T15327	T15328	arg1Of	accumulation,was
R12318	T15327	T15330	arg2Of	accumulation,retarded
R12319	T15330	T15328	arg2Of	retarded,was
R12320	T15330	T15329	arg1Of	retarded,substantially
R12321	T15330	T15331	arg1Of	retarded,by
R12322	T15332	T15331	arg2Of	infecting,by
R12323	T15332	T15334	arg1Of	infecting,with
R12324	T15333	T15332	arg2Of	cells,infecting
R12325	T15338	T15335	arg1Of	O157,wild-type
R12326	T15338	T15336	arg1Of	O157,E.
R12327	T15338	T15337	arg1Of	O157,coli
R12328	T15338	T15339	arg1Of	O157,:
R12329	T15339	T15334	arg2Of	:,with
R12330	T15340	T15339	arg2Of	H7,:
R12331	T15340	T15341	arg1Of	H7,(
R12332	T15343	T15341	arg2Of	7d,(
R12333	T15343	T15342	arg1Of	7d,Fig.
R12334	T15344	T15341	arg3Of	),(
R12335	T15346	T15345	arg2Of	contrast,In
R12336	T15348	T15345	arg1Of	infecting,In
R12337	T15348	T15347	arg1Of	infecting,","
R12338	T15348	T15349	arg1Of	infecting,with
R12339	T15351	T15352	arg1Of	ΔnleH1,or
R12340	T15352	T15349	arg2Of	or,with
R12341	T15352	T15350	arg1Of	or,either
R12342	T15352	T15361	arg1Of	or,in
R12343	T15356	T15355	arg1Of	slightly,only
R12344	T15357	T15356	arg1Of	impaired,slightly
R12345	T15360	T15352	arg2Of	translocation,or
R12346	T15360	T15353	arg1Of	translocation,ΔescN
R12347	T15360	T15354	arg1Of	translocation,strains
R12348	T15360	T15357	arg1Of	translocation,impaired
R12349	T15360	T15358	arg1Of	translocation,RPS3
R12350	T15360	T15359	arg1Of	translocation,nuclear
R12351	T15363	T15364	arg1Of	IKKβ-,or
R12352	T15364	T15361	arg2Of	or,in
R12353	T15364	T15362	arg1Of	or,either
R12354	T15367	T15366	arg2Of	SSEE,(
R12355	T15368	T15366	arg3Of	),(
R12356	T15370	T15364	arg2Of	cells,or
R12357	T15370	T15365	arg1Of	cells,IKKβ
R12358	T15370	T15366	arg1Of	cells,(
R12359	T15370	T15369	arg1Of	cells,-expressing
R12360	T15370	T15371	arg1Of	cells,(
R12361	T15373	T15371	arg2Of	7d,(
R12362	T15373	T15372	arg1Of	7d,Fig.
R12363	T15374	T15371	arg3Of	),(
R12364	T15376	T15375	arg2Of	expected,As
R12365	T15379	T15378	arg1Of	coli,E.
R12366	T15380	T15379	arg1Of	infections,coli
R12367	T15380	T15381	arg1Of	infections,did
R12368	T15380	T15383	arg1Of	infections,affect
R12369	T15383	T15375	arg1Of	affect,As
R12370	T15383	T15377	arg1Of	affect,","
R12371	T15383	T15381	arg2Of	affect,did
R12372	T15383	T15382	arg1Of	affect,not
R12373	T15387	T15383	arg2Of	translocation,affect
R12374	T15387	T15384	arg1Of	translocation,the
R12375	T15387	T15385	arg1Of	translocation,RPS3
R12376	T15387	T15386	arg1Of	translocation,nuclear
R12377	T15387	T15388	arg1Of	translocation,in
R12378	T15391	T15390	arg2Of	SSAA,(
R12379	T15392	T15390	arg3Of	),(
R12380	T15394	T15388	arg2Of	cells,in
R12381	T15394	T15389	arg1Of	cells,IKKβ
R12382	T15394	T15390	arg1Of	cells,(
R12383	T15394	T15393	arg1Of	cells,-expressing
R12384	T15394	T15395	arg1Of	cells,","
R12385	T15394	T15396	arg1Of	cells,where
R12386	T15394	T15401	arg1Of	cells,(
R12387	T15398	T15397	arg1Of	signaling,NF-κB
R12388	T15398	T15399	arg1Of	signaling,was
R12389	T15398	T15400	arg1Of	signaling,low
R12390	T15399	T15396	arg2Of	was,where
R12391	T15400	T15399	arg2Of	low,was
R12392	T15403	T15401	arg2Of	7d,(
R12393	T15403	T15402	arg1Of	7d,Fig.
R12394	T15404	T15401	arg3Of	),(
R12395	T15408	T15407	arg2Of	infection,during
R12396	T15409	T15410	arg1Of	NleH1,is
R12397	T15409	T15412	arg1Of	NleH1,potent
R12398	T15410	T15405	arg1Of	is,Thus
R12399	T15410	T15406	arg1Of	is,","
R12400	T15410	T15407	arg1Of	is,during
R12401	T15412	T15410	arg2Of	potent,is
R12402	T15412	T15411	arg1Of	potent,sufficiently
R12403	T15412	T15413	modOf	potent,to
R12404	T15414	T15413	arg1Of	inhibit,to
R12405	T15414	T15419	arg1Of	inhibit,in
R12406	T15417	T15414	arg2Of	translocation,inhibit
R12407	T15417	T15415	arg1Of	translocation,RPS3
R12408	T15417	T15416	arg1Of	translocation,nuclear
R12409	T15419	T15418	arg1Of	in,even
R12410	T15420	T15419	arg2Of	cells,in
R12411	T15420	T15421	arg1Of	cells,expressing
R12412	T15423	T15421	arg2Of	IKKβ,expressing
R12413	T15423	T15422	arg1Of	IKKβ,constitutively-activated
R12414	T15424	T15425	arg1Of	We,examined
R12415	T15427	T15428	arg1Of	NleH1,could
R12416	T15427	T15430	arg1Of	NleH1,phosphorylate
R12417	T15430	T15425	arg2Of	phosphorylate,examined
R12418	T15430	T15426	arg1Of	phosphorylate,whether
R12419	T15430	T15428	arg2Of	phosphorylate,could
R12420	T15430	T15429	arg1Of	phosphorylate,directly
R12421	T15431	T15430	arg2Of	IKKβ,phosphorylate
R12422	T15431	T15433	arg1Of	IKKβ,inhibiting
R12423	T15433	T15432	arg1Of	inhibiting,thus
R12424	T15437	T15433	arg2Of	phosphorylation,inhibiting
R12425	T15437	T15434	arg1Of	phosphorylation,IKKβ-mediated
R12426	T15437	T15435	arg1Of	phosphorylation,RPS3
R12427	T15437	T15436	arg1Of	phosphorylation,S209
R12428	T15438	T15439	arg1Of	We,performed
R12429	T15439	T15450	arg1Of	performed,as
R12430	T15439	T15457	arg1Of	performed,","
R12431	T15439	T15458	arg1Of	performed,so
R12432	T15441	T15440	arg1Of	vitro,in
R12433	T15443	T15439	arg2Of	assays,performed
R12434	T15443	T15441	arg1Of	assays,vitro
R12435	T15443	T15442	arg1Of	assays,kinase
R12436	T15443	T15444	arg1Of	assays,using
R12437	T15446	T15444	arg2Of	Flag-IKKβ,using
R12438	T15446	T15445	arg2Of	Flag-IKKβ,immunoprecipitated
R12439	T15446	T15447	arg1Of	Flag-IKKβ,(
R12440	T15448	T15447	arg2Of	K44A,(
R12441	T15449	T15447	arg3Of	),(
R12442	T15451	T15452	arg1Of	substrate,and
R12443	T15452	T15450	arg2Of	and,as
R12444	T15452	T15455	arg1Of	and,as
R12445	T15454	T15452	arg2Of	His-NleH1,and
R12446	T15454	T15453	arg1Of	His-NleH1,recombinant
R12447	T15456	T15455	arg2Of	kinase,as
R12448	T15458	T15459	arg1Of	so,that
R12449	T15461	T15460	arg1Of	autophosphorylation,IKKβ
R12450	T15461	T15462	arg1Of	autophosphorylation,would
R12451	T15461	T15464	arg1Of	autophosphorylation,obscure
R12452	T15464	T15458	arg2Of	obscure,so
R12453	T15464	T15462	arg2Of	obscure,would
R12454	T15464	T15463	arg1Of	obscure,not
R12455	T15466	T15464	arg2Of	phosphorylation,obscure
R12456	T15466	T15465	arg1Of	phosphorylation,NleH1-induced
R12457	T15469	T15470	arg1Of	we,did
R12458	T15469	T15472	arg1Of	we,observe
R12459	T15469	T15486	arg1Of	we,ruling
R12460	T15472	T15467	arg1Of	observe,However
R12461	T15472	T15468	arg1Of	observe,","
R12462	T15472	T15470	arg2Of	observe,did
R12463	T15472	T15471	arg1Of	observe,not
R12464	T15472	T15477	arg1Of	observe,in
R12465	T15472	T15484	arg1Of	observe,","
R12466	T15472	T15486	modOf	observe,ruling
R12467	T15476	T15472	arg2Of	incorporation,observe
R12468	T15476	T15473	arg1Of	incorporation,any
R12469	T15476	T15474	arg1Of	incorporation,detectable
R12470	T15476	T15475	arg1Of	incorporation,32P
R12471	T15478	T15477	arg2Of	IKKβ,in
R12472	T15478	T15479	arg1Of	IKKβ,(
R12473	T15481	T15479	arg2Of	Fig.,(
R12474	T15481	T15480	arg1Of	Fig.,Supplementary
R12475	T15481	T15482	arg1Of	Fig.,13
R12476	T15483	T15479	arg3Of	),(
R12477	T15486	T15485	arg1Of	ruling,thus
R12478	T15486	T15487	arg1Of	ruling,out
R12479	T15489	T15486	arg2Of	possibility,ruling
R12480	T15489	T15488	arg1Of	possibility,this
R12481	T15490	T15492	arg1Of	We,tested
R12482	T15492	T15491	arg1Of	tested,then
R12483	T15494	T15492	arg2Of	hypothesis,tested
R12484	T15494	T15493	arg1Of	hypothesis,the
R12485	T15496	T15497	arg1Of	NleH1,could
R12486	T15496	T15498	arg1Of	NleH1,alter
R12487	T15498	T15494	arg2Of	alter,hypothesis
R12488	T15498	T15495	arg1Of	alter,that
R12489	T15498	T15497	arg2Of	alter,could
R12490	T15502	T15498	arg2Of	specificities,alter
R12491	T15502	T15499	arg1Of	specificities,the
R12492	T15502	T15500	arg1Of	specificities,IKKβ
R12493	T15502	T15501	arg1Of	specificities,substrate
R12494	T15505	T15503	arg2Of	end,To
R12495	T15505	T15504	arg1Of	end,this
R12496	T15507	T15508	arg1Of	we,performed
R12497	T15508	T15503	arg1Of	performed,To
R12498	T15508	T15506	arg1Of	performed,","
R12499	T15510	T15509	arg1Of	vitro,in
R12500	T15512	T15508	arg2Of	assays,performed
R12501	T15512	T15510	arg1Of	assays,vitro
R12502	T15512	T15511	arg1Of	assays,kinase
R12503	T15512	T15513	arg1Of	assays,using
R12504	T15515	T15516	arg1Of	CK2,and
R12505	T15517	T15516	arg2Of	IKK,and
R12506	T15518	T15513	arg2Of	substrates,using
R12507	T15518	T15514	arg1Of	substrates,both
R12508	T15518	T15515	arg1Of	substrates,CK2
R12509	T15518	T15517	arg1Of	substrates,IKK
R12510	T15518	T15519	arg1Of	substrates,for
R12511	T15520	T15519	arg2Of	IKKβ,for
R12512	T15522	T15521	arg2Of	expected,As
R12513	T15526	T15524	arg1Of	RPS3,IKKβ
R12514	T15526	T15525	arg2Of	RPS3,phosphorylated
R12515	T15526	T15527	arg1Of	RPS3,(
R12516	T15526	T15534	arg1Of	RPS3,and
R12517	T15529	T15527	arg2Of	7e,(
R12518	T15529	T15528	arg1Of	7e,Fig.
R12519	T15529	T15530	arg1Of	7e,","
R12520	T15531	T15530	arg2Of	lane,","
R12521	T15531	T15532	arg1Of	lane,7
R12522	T15533	T15527	arg3Of	),(
R12523	T15534	T15540	arg1Of	and,(
R12524	T15534	T15548	arg1Of	and,demonstrating
R12525	T15537	T15536	arg2Of	1-54,(
R12526	T15538	T15536	arg3Of	),(
R12527	T15539	T15534	arg2Of	protein,and
R12528	T15539	T15535	arg1Of	protein,GST-IκBα
R12529	T15539	T15536	arg1Of	protein,(
R12530	T15542	T15540	arg2Of	7e,(
R12531	T15542	T15541	arg1Of	7e,Fig.
R12532	T15542	T15543	arg1Of	7e,","
R12533	T15544	T15543	arg2Of	lane,","
R12534	T15544	T15545	arg1Of	lane,9
R12535	T15546	T15540	arg3Of	),(
R12536	T15548	T15521	arg1Of	demonstrating,As
R12537	T15548	T15523	arg1Of	demonstrating,","
R12538	T15548	T15547	arg1Of	demonstrating,","
R12539	T15549	T15550	arg1Of	it,harbors
R12540	T15550	T15548	arg2Of	harbors,demonstrating
R12541	T15552	T15553	arg1Of	CK2,or
R12542	T15553	T15550	arg2Of	or,harbors
R12543	T15553	T15551	arg1Of	or,either
R12544	T15556	T15553	arg2Of	specificity,or
R12545	T15556	T15554	arg1Of	specificity,IKK
R12546	T15556	T15555	arg1Of	specificity,substrate
R12547	T15557	T15558	arg1Of	Preincubation,of
R12548	T15557	T15560	arg1Of	Preincubation,with
R12549	T15557	T15562	arg1Of	Preincubation,reduced
R12550	T15559	T15558	arg2Of	IKKβ,of
R12551	T15561	T15560	arg2Of	NleH1,with
R12552	T15565	T15563	arg1Of	phosphorylation,IKKβ-mediated
R12553	T15565	T15564	arg1Of	phosphorylation,RPS3
R12554	T15565	T15566	arg1Of	phosphorylation,","
R12555	T15565	T15573	arg1Of	phosphorylation,but
R12556	T15571	T15566	arg2Of	specificity,","
R12557	T15571	T15567	arg1Of	specificity,i.e.
R12558	T15571	T15568	arg1Of	specificity,the
R12559	T15571	T15569	arg1Of	specificity,CK2
R12560	T15571	T15570	arg1Of	specificity,kinase
R12561	T15573	T15562	arg2Of	but,reduced
R12562	T15573	T15572	arg1Of	but,","
R12563	T15573	T15574	arg1Of	but,not
R12564	T15577	T15573	arg2Of	phosphorylation,but
R12565	T15577	T15575	arg1Of	phosphorylation,IKKβ-mediated
R12566	T15577	T15576	arg1Of	phosphorylation,GST-IκBα
R12567	T15577	T15578	arg1Of	phosphorylation,","
R12568	T15583	T15578	arg2Of	specificity,","
R12569	T15583	T15579	arg1Of	specificity,i.e.
R12570	T15583	T15580	arg1Of	specificity,the
R12571	T15583	T15581	arg1Of	specificity,IKK
R12572	T15583	T15582	arg1Of	specificity,kinase
R12573	T15583	T15584	arg1Of	specificity,(
R12574	T15586	T15584	arg2Of	7e,(
R12575	T15586	T15585	arg1Of	7e,Fig.
R12576	T15587	T15584	arg3Of	),(
R12577	T15589	T15588	arg1Of	experiments,Control
R12578	T15589	T15590	arg1Of	experiments,revealed
R12579	T15593	T15592	arg1Of	phosphorylation,NleH1-mediated
R12580	T15593	T15594	arg1Of	phosphorylation,or
R12581	T15594	T15591	arg1Of	or,no
R12582	T15594	T15598	arg1Of	or,or
R12583	T15595	T15594	arg2Of	autophosphorylation,or
R12584	T15595	T15596	arg1Of	autophosphorylation,of
R12585	T15597	T15596	arg2Of	RPS3,of
R12586	T15598	T15590	arg2Of	or,revealed
R12587	T15599	T15598	arg2Of	GST-IκBα,or
R12588	T15599	T15600	arg1Of	GST-IκBα,(
R12589	T15602	T15600	arg2Of	7e,(
R12590	T15602	T15601	arg1Of	7e,Fig.
R12591	T15603	T15600	arg3Of	),(
R12592	T15604	T15605	arg1Of	Taken,together
R12593	T15607	T15608	arg1Of	NleH1,blocks
R12594	T15608	T15604	modOf	blocks,Taken
R12595	T15608	T15606	arg1Of	blocks,","
R12596	T15612	T15608	arg2Of	specificity,blocks
R12597	T15612	T15609	arg1Of	specificity,the
R12598	T15612	T15610	arg1Of	specificity,CK2
R12599	T15612	T15611	arg1Of	specificity,substrate
R12600	T15612	T15613	arg1Of	specificity,of
R12601	T15614	T15613	arg2Of	IKKβ,of
R12602	T15616	T15608	arg3Of	inhibiting,blocks
R12603	T15616	T15615	arg1Of	inhibiting,thus
R12604	T15621	T15616	arg2Of	phosphorylation,inhibiting
R12605	T15621	T15617	arg1Of	phosphorylation,the
R12606	T15621	T15618	arg1Of	phosphorylation,IKKβ-mediated
R12607	T15621	T15619	arg1Of	phosphorylation,RPS3
R12608	T15621	T15620	arg1Of	phosphorylation,S209
R12609	T15621	T15623	arg1Of	phosphorylation,representing
R12610	T15623	T15622	arg1Of	representing,thus
R12611	T15623	T15626	arg1Of	representing,strategy
R12612	T15623	T15627	arg1Of	representing,by
R12613	T15623	T15631	arg1Of	representing,:
R12614	T15626	T15624	arg1Of	strategy,a
R12615	T15626	T15625	arg1Of	strategy,novel
R12616	T15630	T15627	arg2Of	O157,by
R12617	T15630	T15628	arg1Of	O157,E.
R12618	T15630	T15629	arg1Of	O157,coli
R12619	T15632	T15623	arg2Of	H7,representing
R12620	T15632	T15633	modOf	H7,to
R12621	T15634	T15633	arg1Of	alter,to
R12622	T15639	T15634	arg2Of	response,alter
R12623	T15639	T15635	arg1Of	response,the
R12624	T15639	T15636	arg1Of	response,host
R12625	T15639	T15637	arg1Of	response,innate
R12626	T15639	T15638	arg1Of	response,immune

bionlp-st-ge-2016-coref

Id	Subject	Object	Predicate	Lexical cue
T15644	3753-3755	Anaphor	denotes	it
T15645	3669-3673	Antecedent	denotes	RPS3
T15640	751-753	Anaphor	denotes	do
T15641	669-676	Antecedent	denotes	reduced
T15642	2080-2099	Anaphor	denotes	inhibitory activity
T15643	2018-2023	Antecedent	denotes	block
R12627	T15640	T15641	boundBy	do,reduced
R12628	T15642	T15643	boundBy	inhibitory activity,block
R12629	T15644	T15645	boundBy	it,RPS3

bionlp-st-ge-2016-spacy-parsed

Id	Subject	Object	Predicate	Lexical cue
T15728	0-5	JJ	denotes	NleH1
T15729	6-12	VBZ	denotes	steers
T15730	13-16	DT	denotes	the
T15731	17-21	NNP	denotes	IKKβ
T15732	22-31	VB	denotes	substrate
T15733	32-45	NNS	denotes	specificities
T15734	46-51	NNP	denotes	NleH1
T15735	52-54	VBZ	denotes	is
T15736	55-57	DT	denotes	an
T15737	58-76	JJ	denotes	autophosphorylated
T15738	77-93	JJ	denotes	serine-threonine
T15739	94-100	NN	denotes	kinase
T15740	100-101	,	denotes	,
T15741	102-107	WDT	denotes	which
T15742	108-115	VBZ	denotes	depends
T15743	116-118	IN	denotes	on
T15744	119-122	DT	denotes	the
T15745	123-129	NN	denotes	lysine
T15746	130-133	CD	denotes	159
T15747	134-135	-LRB-	denotes	(
T15748	135-139	NNP	denotes	K159
T15749	139-140	-RRB-	denotes	)
T15750	140-141	CD	denotes	9
T15751	141-142	.	denotes	.
T15752	143-145	TO	denotes	To
T15753	146-153	VB	denotes	explore
T15754	154-157	DT	denotes	the
T15755	158-167	NN	denotes	mechanism
T15756	168-170	IN	denotes	by
T15757	171-176	WDT	denotes	which
T15758	177-182	NNP	denotes	NleH1
T15759	183-191	VBZ	denotes	inhibits
T15760	192-196	NNP	denotes	RPS3
T15761	197-201	NNP	denotes	S209
T15762	202-217	NN	denotes	phosphorylation
T15763	217-218	,	denotes	,
T15764	219-221	PRP	denotes	we
T15765	222-227	RB	denotes	first
T15766	228-237	VBD	denotes	performed
T15767	238-240	DT	denotes	an
T15768	241-243	IN	denotes	in
T15769	244-249	NN	denotes	vitro
T15770	250-256	NN	denotes	kinase
T15771	257-262	NN	denotes	assay
T15772	263-267	IN	denotes	with
T15773	268-276	JJ	denotes	purified
T15774	277-286	JJ	denotes	wild-type
T15775	287-296	NN	denotes	His-NleH1
T15776	297-304	NN	denotes	protein
T15777	305-308	CC	denotes	and
T15778	309-310	DT	denotes	a
T15779	311-317	JJ	denotes	mutant
T15780	318-327	NN	denotes	His-NleH1
T15781	328-329	-LRB-	denotes	(
T15782	329-334	NNP	denotes	K159A
T15783	334-335	-RRB-	denotes	)
T15784	336-343	NN	denotes	protein
T15785	343-344	,	denotes	,
T15786	345-355	VBG	denotes	confirming
T15787	356-360	IN	denotes	that
T15788	361-366	NNP	denotes	NleH1
T15789	367-369	VBZ	denotes	is
T15790	370-388	VBN	denotes	autophosphorylated
T15791	389-392	CC	denotes	and
T15792	393-396	DT	denotes	the
T15793	397-402	NNP	denotes	K159A
T15794	403-405	VBZ	denotes	is
T15795	406-408	DT	denotes	an
T15796	409-414	JJ	denotes	NleH1
T15797	415-426	NN	denotes	kinase-dead
T15798	427-433	JJ	denotes	mutant
T15799	434-435	-LRB-	denotes	(
T15800	435-439	NNP	denotes	Fig.
T15801	440-442	CD	denotes	7a
T15802	442-443	-RRB-	denotes	)
T15803	443-444	.	denotes	.
T15804	445-447	TO	denotes	To
T15805	448-455	VB	denotes	examine
T15806	456-463	IN	denotes	whether
T15807	464-467	DT	denotes	the
T15808	468-474	NN	denotes	kinase
T15809	475-483	NN	denotes	activity
T15810	484-486	VBZ	denotes	is
T15811	487-495	VBN	denotes	required
T15812	496-499	IN	denotes	for
T15813	500-505	NNP	denotes	NleH1
T15814	506-508	TO	denotes	to
T15815	509-516	VB	denotes	inhibit
T15816	517-521	NNP	denotes	IKKβ
T15817	522-537	NN	denotes	phosphorlyation
T15818	538-540	IN	denotes	of
T15819	541-545	NNP	denotes	RPS3
T15820	546-548	IN	denotes	on
T15821	549-553	NNP	denotes	S209
T15822	553-554	,	denotes	,
T15823	555-557	PRP	denotes	we
T15824	558-569	RB	denotes	ectopically
T15825	570-580	VBG	denotes	expressing
T15826	581-587	CC	denotes	either
T15827	588-597	JJ	denotes	wild-type
T15828	598-600	CC	denotes	or
T15829	601-606	NNP	denotes	K159A
T15830	607-612	NNP	denotes	NleH1
T15831	613-615	IN	denotes	in
T15832	616-620	NNP	denotes	293T
T15833	621-626	NNS	denotes	cells
T15834	626-627	.	denotes	.
T15835	628-637	JJ	denotes	Wild-type
T15836	638-643	NNP	denotes	NleH1
T15837	644-654	NN	denotes	expression
T15838	655-668	RB	denotes	significantly
T15839	669-676	VBD	denotes	reduced
T15840	677-688	JJ	denotes	TNF-induced
T15841	689-693	NNP	denotes	RPS3
T15842	694-698	NNP	denotes	S209
T15843	699-714	NN	denotes	phosphorylation
T15844	714-715	,	denotes	,
T15845	716-723	IN	denotes	whereas
T15846	724-727	DT	denotes	the
T15847	728-733	NNP	denotes	K159A
T15848	734-740	JJ	denotes	mutant
T15849	741-747	VBD	denotes	failed
T15850	748-750	TO	denotes	to
T15851	751-753	VB	denotes	do
T15852	754-756	RB	denotes	so
T15853	757-758	-LRB-	denotes	(
T15854	758-762	NNP	denotes	Fig.
T15855	763-765	CD	denotes	7b
T15856	765-766	-RRB-	denotes	)
T15857	766-767	.	denotes	.
T15858	768-772	RB	denotes	Thus
T15859	773-778	JJ	denotes	NleH1
T15860	779-785	NN	denotes	kinase
T15861	786-794	NN	denotes	activity
T15862	795-797	VBZ	denotes	is
T15863	798-806	VBN	denotes	required
T15864	807-809	TO	denotes	to
T15865	810-817	VB	denotes	protect
T15866	818-822	CD	denotes	RPS3
T15867	823-827	IN	denotes	from
T15868	828-841	JJ	denotes	IKKβ-mediated
T15869	842-857	NN	denotes	phosphorylation
T15870	857-858	.	denotes	.
T15871	859-870	NNP	denotes	Citrobacter
T15872	871-880	NN	denotes	rodentium
T15873	881-883	VBZ	denotes	is
T15874	884-885	DT	denotes	a
T15875	886-891	NN	denotes	mouse
T15876	892-900	NN	denotes	pathogen
T15877	901-905	WDT	denotes	that
T15878	906-912	VBZ	denotes	shares
T15879	913-923	JJ	denotes	pathogenic
T15880	924-934	NNS	denotes	strategies
T15881	935-939	IN	denotes	with
T15882	940-942	NNP	denotes	E.
T15883	943-947	NNS	denotes	coli
T15884	948-950	CD	denotes	39
T15885	950-951	,	denotes	,
T15886	952-956	RBS	denotes	most
T15887	957-964	RB	denotes	notably
T15888	965-968	IN	denotes	for
T15889	969-972	PRP$	denotes	our
T15890	973-986	NN	denotes	investigation
T15891	986-987	,	denotes	,
T15892	988-990	NNP	denotes	C.
T15893	991-1000	NN	denotes	rodentium
T15894	1001-1005	NNP	denotes	NleH
T15895	1006-1015	VBD	denotes	inhibited
T15896	1016-1020	CD	denotes	RPS3
T15897	1021-1028	JJ	denotes	nuclear
T15898	1029-1042	NN	denotes	translocation
T15899	1043-1046	CC	denotes	and
T15900	1047-1061	JJ	denotes	RPS3-dependent
T15901	1062-1067	NN	denotes	NF-κB
T15902	1068-1078	NN	denotes	luciferase
T15903	1079-1087	NN	denotes	activity
T15904	1088-1090	TO	denotes	to
T15905	1091-1093	DT	denotes	an
T15906	1094-1100	NN	denotes	extent
T15907	1101-1111	NN	denotes	equivalent
T15908	1112-1114	TO	denotes	to
T15909	1115-1117	NNP	denotes	E.
T15910	1118-1122	NNS	denotes	coli
T15911	1123-1128	NNP	denotes	NleH1
T15912	1129-1130	-LRB-	denotes	(
T15913	1130-1133	NN	denotes	ref
T15914	1133-1134	.	denotes	.
T15915	1135-1136	CD	denotes	9
T15916	1136-1137	-RRB-	denotes	)
T15917	1137-1138	.	denotes	.
T15918	1139-1141	PRP	denotes	We
T15919	1142-1149	VBD	denotes	assayed
T15920	1150-1154	NNP	denotes	RPS3
T15921	1155-1159	NNP	denotes	S209
T15922	1160-1175	NN	denotes	phosphorylation
T15923	1176-1178	IN	denotes	in
T15924	1179-1183	NNP	denotes	HeLa
T15925	1184-1189	NNS	denotes	cells
T15926	1190-1198	VBN	denotes	infected
T15927	1199-1203	IN	denotes	with
T15928	1204-1213	JJ	denotes	different
T15929	1214-1216	NNP	denotes	C.
T15930	1217-1226	NN	denotes	rodentium
T15931	1227-1234	NNS	denotes	strains
T15932	1234-1235	.	denotes	.
T15933	1236-1238	IN	denotes	In
T15934	1239-1249	JJ	denotes	uninfected
T15935	1250-1255	NNS	denotes	cells
T15936	1255-1256	,	denotes	,
T15937	1257-1270	JJ	denotes	TNF-treatment
T15938	1271-1281	VBD	denotes	stimulated
T15939	1282-1283	DT	denotes	a
T15940	1284-1285	NN	denotes	∼
T15941	1285-1293	JJ	denotes	3.5-fold
T15942	1294-1302	NN	denotes	increase
T15943	1303-1305	IN	denotes	in
T15944	1306-1310	NNP	denotes	RPS3
T15945	1311-1315	NNP	denotes	S209
T15946	1316-1331	NN	denotes	phosphorylation
T15947	1332-1333	-LRB-	denotes	(
T15948	1333-1337	NNP	denotes	Fig.
T15949	1338-1340	CD	denotes	7c
T15950	1340-1341	-RRB-	denotes	)
T15951	1341-1342	.	denotes	.
T15952	1343-1347	JJ	denotes	Such
T15953	1348-1360	NN	denotes	augmentation
T15954	1361-1363	IN	denotes	of
T15955	1364-1368	CD	denotes	RPS3
T15956	1369-1384	NN	denotes	phosphorylation
T15957	1385-1388	VBD	denotes	was
T15958	1389-1396	VBN	denotes	reduced
T15959	1397-1399	IN	denotes	by
T15960	1400-1405	RB	denotes	about
T15961	1406-1408	CD	denotes	60
T15962	1408-1409	NN	denotes	%
T15963	1410-1412	IN	denotes	by
T15964	1413-1422	JJ	denotes	wild-type
T15965	1423-1425	NNP	denotes	C.
T15966	1426-1435	NN	denotes	rodentium
T15967	1436-1445	NN	denotes	infection
T15968	1446-1447	-LRB-	denotes	(
T15969	1447-1451	NNP	denotes	Fig.
T15970	1452-1454	CD	denotes	7c
T15971	1454-1455	-RRB-	denotes	)
T15972	1455-1456	.	denotes	.
T15973	1457-1464	RB	denotes	However
T15974	1464-1465	,	denotes	,
T15975	1466-1470	JJ	denotes	RPS3
T15976	1471-1486	NN	denotes	phosphorylation
T15977	1487-1490	VBD	denotes	was
T15978	1491-1499	VBN	denotes	enhanced
T15979	1500-1504	WRB	denotes	when
T15980	1505-1510	NNS	denotes	cells
T15981	1511-1515	VBD	denotes	were
T15982	1516-1524	VBN	denotes	infected
T15983	1525-1529	IN	denotes	with
T15984	1530-1531	DT	denotes	a
T15985	1532-1534	NNP	denotes	C.
T15986	1535-1544	NN	denotes	rodentium
T15987	1545-1551	NN	denotes	strain
T15988	1552-1559	VBG	denotes	lacking
T15989	1560-1564	NNP	denotes	NleH
T15990	1565-1566	-LRB-	denotes	(
T15991	1566-1570	NNP	denotes	Fig.
T15992	1571-1573	CD	denotes	7c
T15993	1573-1574	,	denotes	,
T15994	1575-1580	NNP	denotes	ΔnleH
T15995	1580-1581	-RRB-	denotes	)
T15996	1581-1582	.	denotes	.
T15997	1583-1585	PRP	denotes	We
T15998	1586-1593	RB	denotes	further
T15999	1594-1602	VBD	denotes	examined
T16000	1603-1606	DT	denotes	the
T16001	1607-1611	NN	denotes	role
T16002	1612-1614	IN	denotes	of
T16003	1615-1620	JJ	denotes	NleH1
T16004	1621-1627	NN	denotes	kinase
T16005	1628-1636	NN	denotes	activity
T16006	1637-1642	VBG	denotes	using
T16007	1643-1646	DT	denotes	the
T16008	1647-1649	NNP	denotes	C.
T16009	1650-1659	NN	denotes	rodentium
T16010	1660-1665	NNP	denotes	ΔnleH
T16011	1666-1672	NN	denotes	strain
T16012	1673-1675	IN	denotes	as
T16013	1676-1677	DT	denotes	a
T16014	1678-1688	NN	denotes	background
T16015	1689-1691	IN	denotes	on
T16016	1692-1697	WDT	denotes	which
T16017	1698-1700	TO	denotes	to
T16018	1701-1708	VB	denotes	express
T16019	1709-1715	DT	denotes	either
T16020	1716-1725	JJ	denotes	wild-type
T16021	1726-1728	CC	denotes	or
T16022	1729-1734	NNP	denotes	K159A
T16023	1735-1737	NNP	denotes	E.
T16024	1738-1742	NNS	denotes	coli
T16025	1743-1748	NNP	denotes	NleH1
T16026	1748-1749	.	denotes	.
T16027	1750-1763	VBG	denotes	Complementing
T16028	1764-1769	NNP	denotes	ΔnleH
T16029	1770-1776	JJ	denotes	mutant
T16030	1777-1781	IN	denotes	with
T16031	1782-1791	JJ	denotes	wild-type
T16032	1792-1797	NNP	denotes	NleH1
T16033	1798-1804	RB	denotes	almost
T16034	1805-1814	VBD	denotes	abolished
T16035	1815-1826	JJ	denotes	TNF-induced
T16036	1827-1831	NNP	denotes	RPS3
T16037	1832-1836	NNP	denotes	S209
T16038	1837-1852	NN	denotes	phosphorylation
T16039	1853-1860	IN	denotes	whereas
T16040	1861-1874	VBG	denotes	complementing
T16041	1875-1879	IN	denotes	with
T16042	1880-1885	NNP	denotes	K159A
T16043	1886-1892	VBD	denotes	failed
T16044	1893-1895	TO	denotes	to
T16045	1896-1903	VB	denotes	inhibit
T16046	1904-1908	JJ	denotes	RPS3
T16047	1909-1924	NN	denotes	phosphorylation
T16048	1925-1926	-LRB-	denotes	(
T16049	1926-1930	NNP	denotes	Fig.
T16050	1931-1933	CD	denotes	7c
T16051	1933-1934	-RRB-	denotes	)
T16052	1934-1935	.	denotes	.
T16053	1936-1948	RB	denotes	Collectively
T16054	1948-1949	,	denotes	,
T16055	1950-1955	DT	denotes	these
T16056	1956-1963	NNS	denotes	results
T16057	1964-1975	VBP	denotes	demonstrate
T16058	1976-1980	IN	denotes	that
T16059	1981-1986	JJ	denotes	NleH1
T16060	1987-1993	NN	denotes	kinase
T16061	1994-2002	NN	denotes	activity
T16062	2003-2005	VBZ	denotes	is
T16063	2006-2014	VBN	denotes	required
T16064	2015-2017	TO	denotes	to
T16065	2018-2023	VB	denotes	block
T16066	2024-2028	NNP	denotes	RPS3
T16067	2029-2033	NNP	denotes	S209
T16068	2034-2049	NN	denotes	phosphorylation
T16069	2049-2050	.	denotes	.
T16070	2051-2053	PRP	denotes	We
T16071	2054-2058	JJ	denotes	next
T16072	2059-2067	VBD	denotes	examined
T16073	2068-2075	IN	denotes	whether
T16074	2076-2079	DT	denotes	the
T16075	2080-2090	JJ	denotes	inhibitory
T16076	2091-2099	NN	denotes	activity
T16077	2100-2102	IN	denotes	of
T16078	2103-2108	NNP	denotes	NleH1
T16079	2109-2111	VBZ	denotes	is
T16080	2112-2124	RB	denotes	sufficiently
T16081	2125-2131	JJ	denotes	robust
T16082	2132-2134	TO	denotes	to
T16083	2135-2141	VB	denotes	impair
T16084	2142-2145	DT	denotes	the
T16085	2146-2152	JJ	denotes	strong
T16086	2153-2160	JJ	denotes	nuclear
T16087	2161-2174	NN	denotes	translocation
T16088	2175-2177	IN	denotes	of
T16089	2178-2182	CD	denotes	RPS3
T16090	2183-2190	VBN	denotes	trigged
T16091	2191-2193	IN	denotes	by
T16092	2194-2197	DT	denotes	the
T16093	2198-2219	JJ	denotes	constitutively-active
T16094	2220-2224	NNP	denotes	IKKβ
T16095	2225-2226	-LRB-	denotes	(
T16096	2226-2230	NNP	denotes	IKKβ
T16097	2231-2232	NNP	denotes	[
T16098	2232-2236	NNP	denotes	SSEE
T16099	2236-2237	NNP	denotes	]
T16100	2237-2238	-RRB-	denotes	)
T16101	2239-2240	-LRB-	denotes	(
T16102	2240-2244	NNP	denotes	Fig.
T16103	2245-2247	CD	denotes	2d
T16104	2247-2248	-RRB-	denotes	)
T16105	2248-2249	.	denotes	.
T16106	2250-2252	PRP	denotes	We
T16107	2253-2258	VBD	denotes	found
T16108	2259-2263	IN	denotes	that
T16109	2264-2275	RB	denotes	ectopically
T16110	2276-2286	VBG	denotes	expressing
T16111	2287-2293	CC	denotes	either
T16112	2294-2303	JJ	denotes	wild-type
T16113	2304-2306	CC	denotes	or
T16114	2307-2311	NNP	denotes	SSEE
T16115	2312-2316	NNP	denotes	IKKβ
T16116	2317-2325	NNS	denotes	proteins
T16117	2325-2326	,	denotes	,
T16118	2327-2336	VBD	denotes	triggered
T16119	2337-2341	RBR	denotes	more
T16120	2342-2346	JJ	denotes	RPS3
T16121	2347-2354	JJ	denotes	nuclear
T16122	2355-2368	NN	denotes	translocation
T16123	2369-2373	IN	denotes	than
T16124	2374-2377	DT	denotes	the
T16125	2378-2389	JJ	denotes	kinase-dead
T16126	2390-2394	NNP	denotes	IKKβ
T16127	2395-2396	-LRB-	denotes	(
T16128	2396-2400	NNP	denotes	SSAA
T16129	2400-2401	-RRB-	denotes	)
T16130	2402-2409	NN	denotes	protein
T16131	2410-2411	-LRB-	denotes	(
T16132	2411-2415	NNP	denotes	Fig.
T16133	2416-2418	CD	denotes	7d
T16134	2418-2419	-RRB-	denotes	)
T16135	2419-2420	.	denotes	.
T16136	2421-2425	CD	denotes	RPS3
T16137	2426-2433	JJ	denotes	nuclear
T16138	2434-2446	NN	denotes	accumulation
T16139	2447-2450	VBD	denotes	was
T16140	2451-2464	RB	denotes	substantially
T16141	2465-2473	JJ	denotes	retarded
T16142	2474-2476	IN	denotes	by
T16143	2477-2486	VBG	denotes	infecting
T16144	2487-2492	NNS	denotes	cells
T16145	2493-2497	IN	denotes	with
T16146	2498-2507	JJ	denotes	wild-type
T16147	2508-2510	NNP	denotes	E.
T16148	2511-2515	NNS	denotes	coli
T16149	2516-2520	NNP	denotes	O157
T16150	2520-2521	:	denotes	:
T16151	2521-2523	NNP	denotes	H7
T16152	2524-2525	-LRB-	denotes	(
T16153	2525-2529	NNP	denotes	Fig.
T16154	2530-2532	CD	denotes	7d
T16155	2532-2533	-RRB-	denotes	)
T16156	2533-2534	.	denotes	.
T16157	2535-2537	IN	denotes	In
T16158	2538-2546	NN	denotes	contrast
T16159	2546-2547	,	denotes	,
T16160	2548-2557	VBG	denotes	infecting
T16161	2558-2562	IN	denotes	with
T16162	2563-2569	DT	denotes	either
T16163	2570-2576	NNP	denotes	ΔnleH1
T16164	2577-2579	CC	denotes	or
T16165	2580-2585	NNP	denotes	ΔescN
T16166	2586-2593	NNS	denotes	strains
T16167	2594-2598	RB	denotes	only
T16168	2599-2607	RB	denotes	slightly
T16169	2608-2616	VBN	denotes	impaired
T16170	2617-2621	NNP	denotes	RPS3
T16171	2622-2629	JJ	denotes	nuclear
T16172	2630-2643	NN	denotes	translocation
T16173	2644-2646	IN	denotes	in
T16174	2647-2653	CC	denotes	either
T16175	2654-2658	SYM	denotes	IKKβ
T16176	2658-2659	:	denotes	-
T16177	2660-2662	CC	denotes	or
T16178	2663-2667	NNP	denotes	IKKβ
T16179	2668-2669	-LRB-	denotes	(
T16180	2669-2673	NNP	denotes	SSEE
T16181	2673-2674	-RRB-	denotes	)
T16182	2674-2675	:	denotes	-
T16183	2675-2685	VBG	denotes	expressing
T16184	2686-2691	NNS	denotes	cells
T16185	2692-2693	-LRB-	denotes	(
T16186	2693-2697	NNP	denotes	Fig.
T16187	2698-2700	CD	denotes	7d
T16188	2700-2701	-RRB-	denotes	)
T16189	2701-2702	.	denotes	.
T16190	2703-2705	IN	denotes	As
T16191	2706-2714	VBN	denotes	expected
T16192	2714-2715	,	denotes	,
T16193	2716-2718	NNP	denotes	E.
T16194	2719-2723	NNS	denotes	coli
T16195	2724-2734	NNS	denotes	infections
T16196	2735-2738	VBD	denotes	did
T16197	2739-2742	RB	denotes	not
T16198	2743-2749	VB	denotes	affect
T16199	2750-2753	DT	denotes	the
T16200	2754-2758	JJ	denotes	RPS3
T16201	2759-2766	JJ	denotes	nuclear
T16202	2767-2780	NN	denotes	translocation
T16203	2781-2783	IN	denotes	in
T16204	2784-2788	NNP	denotes	IKKβ
T16205	2789-2790	-LRB-	denotes	(
T16206	2790-2794	NNP	denotes	SSAA
T16207	2794-2795	-RRB-	denotes	)
T16208	2795-2796	:	denotes	-
T16209	2796-2806	VBG	denotes	expressing
T16210	2807-2812	NNS	denotes	cells
T16211	2812-2813	,	denotes	,
T16212	2814-2819	WRB	denotes	where
T16213	2820-2825	JJ	denotes	NF-κB
T16214	2826-2835	VBG	denotes	signaling
T16215	2836-2839	VBD	denotes	was
T16216	2840-2843	JJ	denotes	low
T16217	2844-2845	-LRB-	denotes	(
T16218	2845-2849	NNP	denotes	Fig.
T16219	2850-2852	CD	denotes	7d
T16220	2852-2853	-RRB-	denotes	)
T16221	2853-2854	.	denotes	.
T16222	2855-2859	RB	denotes	Thus
T16223	2859-2860	,	denotes	,
T16224	2861-2867	IN	denotes	during
T16225	2868-2877	NN	denotes	infection
T16226	2878-2883	NNP	denotes	NleH1
T16227	2884-2886	VBZ	denotes	is
T16228	2887-2899	RB	denotes	sufficiently
T16229	2900-2906	JJ	denotes	potent
T16230	2907-2909	TO	denotes	to
T16231	2910-2917	VB	denotes	inhibit
T16232	2918-2922	JJ	denotes	RPS3
T16233	2923-2930	JJ	denotes	nuclear
T16234	2931-2944	NN	denotes	translocation
T16235	2945-2949	RB	denotes	even
T16236	2950-2952	IN	denotes	in
T16237	2953-2958	NNS	denotes	cells
T16238	2959-2969	VBG	denotes	expressing
T16239	2970-2994	JJ	denotes	constitutively-activated
T16240	2995-2999	NNP	denotes	IKKβ
T16241	2999-3000	.	denotes	.
T16242	3001-3003	PRP	denotes	We
T16243	3004-3012	VBD	denotes	examined
T16244	3013-3020	IN	denotes	whether
T16245	3021-3026	NNP	denotes	NleH1
T16246	3027-3032	MD	denotes	could
T16247	3033-3041	RB	denotes	directly
T16248	3042-3055	VB	denotes	phosphorylate
T16249	3056-3060	NNP	denotes	IKKβ
T16250	3061-3065	RB	denotes	thus
T16251	3066-3076	VBG	denotes	inhibiting
T16252	3077-3090	JJ	denotes	IKKβ-mediated
T16253	3091-3095	CD	denotes	RPS3
T16254	3096-3100	CD	denotes	S209
T16255	3101-3116	NN	denotes	phosphorylation
T16256	3116-3117	.	denotes	.
T16257	3118-3120	PRP	denotes	We
T16258	3121-3130	VBD	denotes	performed
T16259	3131-3133	IN	denotes	in
T16260	3134-3139	NN	denotes	vitro
T16261	3140-3146	NN	denotes	kinase
T16262	3147-3153	VBZ	denotes	assays
T16263	3154-3159	VBG	denotes	using
T16264	3160-3178	VBN	denotes	immunoprecipitated
T16265	3179-3188	NNP	denotes	Flag-IKKβ
T16266	3189-3190	-LRB-	denotes	(
T16267	3190-3194	NNP	denotes	K44A
T16268	3194-3195	-RRB-	denotes	)
T16269	3196-3198	IN	denotes	as
T16270	3199-3208	NN	denotes	substrate
T16271	3209-3212	CC	denotes	and
T16272	3213-3224	JJ	denotes	recombinant
T16273	3225-3234	NN	denotes	His-NleH1
T16274	3235-3237	IN	denotes	as
T16275	3238-3244	NN	denotes	kinase
T16276	3244-3245	,	denotes	,
T16277	3246-3248	RB	denotes	so
T16278	3249-3253	IN	denotes	that
T16279	3254-3258	NNP	denotes	IKKβ
T16280	3259-3278	NN	denotes	autophosphorylation
T16281	3279-3284	MD	denotes	would
T16282	3285-3288	RB	denotes	not
T16283	3289-3296	VB	denotes	obscure
T16284	3297-3310	JJ	denotes	NleH1-induced
T16285	3311-3326	NN	denotes	phosphorylation
T16286	3326-3327	.	denotes	.
T16287	3328-3335	RB	denotes	However
T16288	3335-3336	,	denotes	,
T16289	3337-3339	PRP	denotes	we
T16290	3340-3343	VBD	denotes	did
T16291	3344-3347	RB	denotes	not
T16292	3348-3355	VB	denotes	observe
T16293	3356-3359	DT	denotes	any
T16294	3360-3370	JJ	denotes	detectable
T16295	3371-3374	NNP	denotes	32P
T16296	3375-3388	NN	denotes	incorporation
T16297	3389-3391	IN	denotes	in
T16298	3392-3396	NNP	denotes	IKKβ
T16299	3397-3398	-LRB-	denotes	(
T16300	3398-3411	NNP	denotes	Supplementary
T16301	3412-3416	NNP	denotes	Fig.
T16302	3417-3419	CD	denotes	13
T16303	3419-3420	-RRB-	denotes	)
T16304	3420-3421	,	denotes	,
T16305	3422-3426	RB	denotes	thus
T16306	3427-3433	VBG	denotes	ruling
T16307	3434-3437	RP	denotes	out
T16308	3438-3442	DT	denotes	this
T16309	3443-3454	NN	denotes	possibility
T16310	3454-3455	.	denotes	.
T16311	3456-3458	PRP	denotes	We
T16312	3459-3463	RB	denotes	then
T16313	3464-3470	VBD	denotes	tested
T16314	3471-3474	DT	denotes	the
T16315	3475-3485	NN	denotes	hypothesis
T16316	3486-3490	WDT	denotes	that
T16317	3491-3496	NNP	denotes	NleH1
T16318	3497-3502	MD	denotes	could
T16319	3503-3508	VB	denotes	alter
T16320	3509-3512	DT	denotes	the
T16321	3513-3517	NNP	denotes	IKKβ
T16322	3518-3527	NN	denotes	substrate
T16323	3528-3541	NNS	denotes	specificities
T16324	3541-3542	.	denotes	.
T16325	3543-3545	TO	denotes	To
T16326	3546-3550	DT	denotes	this
T16327	3551-3554	NN	denotes	end
T16328	3554-3555	,	denotes	,
T16329	3556-3558	PRP	denotes	we
T16330	3559-3568	VBD	denotes	performed
T16331	3569-3571	IN	denotes	in
T16332	3572-3577	NN	denotes	vitro
T16333	3578-3584	NN	denotes	kinase
T16334	3585-3591	VBZ	denotes	assays
T16335	3592-3597	VBG	denotes	using
T16336	3598-3602	DT	denotes	both
T16337	3603-3606	CD	denotes	CK2
T16338	3607-3610	CC	denotes	and
T16339	3611-3614	NNP	denotes	IKK
T16340	3615-3625	VBZ	denotes	substrates
T16341	3626-3629	IN	denotes	for
T16342	3630-3634	NNP	denotes	IKKβ
T16343	3634-3635	.	denotes	.
T16344	3636-3638	IN	denotes	As
T16345	3639-3647	VBN	denotes	expected
T16346	3647-3648	,	denotes	,
T16347	3649-3653	NNP	denotes	IKKβ
T16348	3654-3668	VBD	denotes	phosphorylated
T16349	3669-3673	NNP	denotes	RPS3
T16350	3674-3675	-LRB-	denotes	(
T16351	3675-3679	NNP	denotes	Fig.
T16352	3680-3682	CD	denotes	7e
T16353	3682-3683	,	denotes	,
T16354	3684-3688	NN	denotes	lane
T16355	3689-3690	CD	denotes	7
T16356	3690-3691	-RRB-	denotes	)
T16357	3692-3695	CC	denotes	and
T16358	3696-3704	NNP	denotes	GST-IκBα
T16359	3705-3706	-LRB-	denotes	(
T16360	3706-3710	CD	denotes	1-54
T16361	3710-3711	-RRB-	denotes	)
T16362	3712-3719	NN	denotes	protein
T16363	3720-3721	-LRB-	denotes	(
T16364	3721-3725	NNP	denotes	Fig.
T16365	3726-3728	CD	denotes	7e
T16366	3728-3729	,	denotes	,
T16367	3730-3734	NN	denotes	lane
T16368	3735-3736	CD	denotes	9
T16369	3736-3737	-RRB-	denotes	)
T16370	3737-3738	,	denotes	,
T16371	3739-3752	VBG	denotes	demonstrating
T16372	3753-3755	PRP	denotes	it
T16373	3756-3763	VBZ	denotes	harbors
T16374	3764-3770	CC	denotes	either
T16375	3771-3774	CD	denotes	CK2
T16376	3775-3777	CC	denotes	or
T16377	3778-3781	NNP	denotes	IKK
T16378	3782-3791	VBP	denotes	substrate
T16379	3792-3803	NN	denotes	specificity
T16380	3803-3804	.	denotes	.
T16381	3805-3818	NNP	denotes	Preincubation
T16382	3819-3821	IN	denotes	of
T16383	3822-3826	NNP	denotes	IKKβ
T16384	3827-3831	IN	denotes	with
T16385	3832-3837	NNP	denotes	NleH1
T16386	3838-3845	VBD	denotes	reduced
T16387	3846-3859	JJ	denotes	IKKβ-mediated
T16388	3860-3864	JJ	denotes	RPS3
T16389	3865-3880	NN	denotes	phosphorylation
T16390	3880-3881	,	denotes	,
T16391	3882-3886	FW	denotes	i.e.
T16392	3887-3890	DT	denotes	the
T16393	3891-3894	NNP	denotes	CK2
T16394	3895-3901	NN	denotes	kinase
T16395	3902-3913	NN	denotes	specificity
T16396	3913-3914	,	denotes	,
T16397	3915-3918	CC	denotes	but
T16398	3919-3922	RB	denotes	not
T16399	3923-3936	JJ	denotes	IKKβ-mediated
T16400	3937-3945	JJ	denotes	GST-IκBα
T16401	3946-3961	NN	denotes	phosphorylation
T16402	3961-3962	,	denotes	,
T16403	3963-3967	FW	denotes	i.e.
T16404	3968-3971	DT	denotes	the
T16405	3972-3975	NNP	denotes	IKK
T16406	3976-3982	NN	denotes	kinase
T16407	3983-3994	NN	denotes	specificity
T16408	3995-3996	-LRB-	denotes	(
T16409	3996-4000	NNP	denotes	Fig.
T16410	4001-4003	CD	denotes	7e
T16411	4003-4004	-RRB-	denotes	)
T16412	4004-4005	.	denotes	.
T16413	4006-4013	NNP	denotes	Control
T16414	4014-4025	NNS	denotes	experiments
T16415	4026-4034	VBD	denotes	revealed
T16416	4035-4037	DT	denotes	no
T16417	4038-4052	JJ	denotes	NleH1-mediated
T16418	4053-4068	NN	denotes	phosphorylation
T16419	4069-4071	CC	denotes	or
T16420	4072-4091	NN	denotes	autophosphorylation
T16421	4092-4094	IN	denotes	of
T16422	4095-4099	CD	denotes	RPS3
T16423	4100-4102	CC	denotes	or
T16424	4103-4111	NNP	denotes	GST-IκBα
T16425	4112-4113	-LRB-	denotes	(
T16426	4113-4117	NNP	denotes	Fig.
T16427	4118-4120	CD	denotes	7e
T16428	4120-4121	-RRB-	denotes	)
T16429	4121-4122	.	denotes	.
T16430	4123-4128	VBN	denotes	Taken
T16431	4129-4137	RB	denotes	together
T16432	4137-4138	,	denotes	,
T16433	4139-4144	JJ	denotes	NleH1
T16434	4145-4151	NNS	denotes	blocks
T16435	4152-4155	DT	denotes	the
T16436	4156-4159	NNP	denotes	CK2
T16437	4160-4169	NN	denotes	substrate
T16438	4170-4181	NN	denotes	specificity
T16439	4182-4184	IN	denotes	of
T16440	4185-4189	NNP	denotes	IKKβ
T16441	4190-4194	RB	denotes	thus
T16442	4195-4205	VBG	denotes	inhibiting
T16443	4206-4209	DT	denotes	the
T16444	4210-4223	JJ	denotes	IKKβ-mediated
T16445	4224-4228	CD	denotes	RPS3
T16446	4229-4233	CD	denotes	S209
T16447	4234-4249	NN	denotes	phosphorylation
T16448	4250-4254	RB	denotes	thus
T16449	4255-4267	VBG	denotes	representing
T16450	4268-4269	DT	denotes	a
T16451	4270-4275	NN	denotes	novel
T16452	4276-4284	NN	denotes	strategy
T16453	4285-4287	IN	denotes	by
T16454	4288-4290	NNP	denotes	E.
T16455	4291-4295	NNS	denotes	coli
T16456	4296-4300	NNP	denotes	O157
T16457	4300-4301	:	denotes	:
T16458	4301-4303	NNP	denotes	H7
T16459	4304-4306	TO	denotes	to
T16460	4307-4312	VB	denotes	alter
T16461	4313-4316	DT	denotes	the
T16462	4317-4321	NN	denotes	host
T16463	4322-4328	NN	denotes	innate
T16464	4329-4335	JJ	denotes	immune
T16465	4336-4344	NN	denotes	response
T16466	4344-4345	.	denotes	.
R12630	T15728	T15729	nsubj	NleH1,steers
R12631	T15729	T15729	ROOT	steers,steers
R12632	T15730	T15733	det	the,specificities
R12633	T15731	T15732	compound	IKKβ,substrate
R12634	T15732	T15733	compound	substrate,specificities
R12635	T15733	T15729	dobj	specificities,steers
R12636	T15734	T15735	nsubj	NleH1,is
R12637	T15735	T15729	conj	is,steers
R12638	T15736	T15739	det	an,kinase
R12639	T15737	T15739	amod	autophosphorylated,kinase
R12640	T15738	T15739	amod	serine-threonine,kinase
R12641	T15739	T15735	attr	kinase,is
R12642	T15740	T15739	punct	",",kinase
R12643	T15741	T15742	nsubj	which,depends
R12644	T15742	T15739	relcl	depends,kinase
R12645	T15743	T15742	prep	on,depends
R12646	T15744	T15750	det	the,9
R12647	T15745	T15750	nmod	lysine,9
R12648	T15746	T15745	nummod	159,lysine
R12649	T15747	T15745	punct	(,lysine
R12650	T15748	T15745	appos	K159,lysine
R12651	T15749	T15750	punct	),9
R12652	T15750	T15743	pobj	9,on
R12653	T15751	T15735	punct	.,is
R12654	T15752	T15753	aux	To,explore
R12655	T15753	T15766	advcl	explore,performed
R12656	T15754	T15755	det	the,mechanism
R12657	T15755	T15753	dobj	mechanism,explore
R12658	T15756	T15759	prep	by,inhibits
R12659	T15757	T15756	pobj	which,by
R12660	T15758	T15759	nsubj	NleH1,inhibits
R12661	T15759	T15755	relcl	inhibits,mechanism
R12662	T15760	T15762	compound	RPS3,phosphorylation
R12663	T15761	T15762	compound	S209,phosphorylation
R12664	T15762	T15759	dobj	phosphorylation,inhibits
R12665	T15763	T15766	punct	",",performed
R12666	T15764	T15766	nsubj	we,performed
R12667	T15765	T15766	advmod	first,performed
R12668	T15766	T15766	ROOT	performed,performed
R12669	T15767	T15771	det	an,assay
R12670	T15768	T15771	nmod	in,assay
R12671	T15769	T15771	amod	vitro,assay
R12672	T15770	T15771	compound	kinase,assay
R12673	T15771	T15766	dobj	assay,performed
R12674	T15772	T15766	prep	with,performed
R12675	T15773	T15776	amod	purified,protein
R12676	T15774	T15776	amod	wild-type,protein
R12677	T15775	T15776	compound	His-NleH1,protein
R12678	T15776	T15772	pobj	protein,with
R12679	T15777	T15776	cc	and,protein
R12680	T15778	T15780	det	a,His-NleH1
R12681	T15779	T15780	amod	mutant,His-NleH1
R12682	T15780	T15776	conj	His-NleH1,protein
R12683	T15781	T15780	punct	(,His-NleH1
R12684	T15782	T15780	appos	K159A,His-NleH1
R12685	T15783	T15782	punct	),K159A
R12686	T15784	T15780	conj	protein,His-NleH1
R12687	T15785	T15766	punct	",",performed
R12688	T15786	T15766	advcl	confirming,performed
R12689	T15787	T15789	mark	that,is
R12690	T15788	T15789	nsubj	NleH1,is
R12691	T15789	T15786	ccomp	is,confirming
R12692	T15790	T15789	acomp	autophosphorylated,is
R12693	T15791	T15790	cc	and,autophosphorylated
R12694	T15792	T15793	det	the,K159A
R12695	T15793	T15794	nsubj	K159A,is
R12696	T15794	T15790	conj	is,autophosphorylated
R12697	T15795	T15798	det	an,mutant
R12698	T15796	T15798	amod	NleH1,mutant
R12699	T15797	T15798	compound	kinase-dead,mutant
R12700	T15798	T15794	attr	mutant,is
R12701	T15799	T15802	punct	(,)
R12702	T15800	T15802	nmod	Fig.,)
R12703	T15801	T15800	nummod	7a,Fig.
R12704	T15802	T15794	punct	),is
R12705	T15803	T15766	punct	.,performed
R12706	T15804	T15805	aux	To,examine
R12707	T15805	T15825	advcl	examine,expressing
R12708	T15806	T15811	mark	whether,required
R12709	T15807	T15809	det	the,activity
R12710	T15808	T15809	compound	kinase,activity
R12711	T15809	T15811	nsubjpass	activity,required
R12712	T15810	T15811	auxpass	is,required
R12713	T15811	T15805	ccomp	required,examine
R12714	T15812	T15815	mark	for,inhibit
R12715	T15813	T15815	nsubj	NleH1,inhibit
R12716	T15814	T15815	aux	to,inhibit
R12717	T15815	T15811	advcl	inhibit,required
R12718	T15816	T15817	compound	IKKβ,phosphorlyation
R12719	T15817	T15815	dobj	phosphorlyation,inhibit
R12720	T15818	T15817	prep	of,phosphorlyation
R12721	T15819	T15818	pobj	RPS3,of
R12722	T15820	T15817	prep	on,phosphorlyation
R12723	T15821	T15820	pobj	S209,on
R12724	T15822	T15825	punct	",",expressing
R12725	T15823	T15825	nsubj	we,expressing
R12726	T15824	T15825	advmod	ectopically,expressing
R12727	T15825	T15825	ROOT	expressing,expressing
R12728	T15826	T15827	preconj	either,wild-type
R12729	T15827	T15825	dobj	wild-type,expressing
R12730	T15828	T15827	cc	or,wild-type
R12731	T15829	T15830	compound	K159A,NleH1
R12732	T15830	T15827	conj	NleH1,wild-type
R12733	T15831	T15830	prep	in,NleH1
R12734	T15832	T15833	compound	293T,cells
R12735	T15833	T15831	pobj	cells,in
R12736	T15834	T15825	punct	.,expressing
R12737	T15835	T15837	amod	Wild-type,expression
R12738	T15836	T15837	compound	NleH1,expression
R12739	T15837	T15839	nsubj	expression,reduced
R12740	T15838	T15839	advmod	significantly,reduced
R12741	T15839	T15839	ROOT	reduced,reduced
R12742	T15840	T15843	amod	TNF-induced,phosphorylation
R12743	T15841	T15843	compound	RPS3,phosphorylation
R12744	T15842	T15843	compound	S209,phosphorylation
R12745	T15843	T15839	dobj	phosphorylation,reduced
R12746	T15844	T15839	punct	",",reduced
R12747	T15845	T15849	mark	whereas,failed
R12748	T15846	T15848	det	the,mutant
R12749	T15847	T15848	compound	K159A,mutant
R12750	T15848	T15849	nsubj	mutant,failed
R12751	T15849	T15839	advcl	failed,reduced
R12752	T15850	T15851	aux	to,do
R12753	T15851	T15849	xcomp	do,failed
R12754	T15852	T15851	advmod	so,do
R12755	T15853	T15854	punct	(,Fig.
R12756	T15854	T15856	nmod	Fig.,)
R12757	T15855	T15854	nummod	7b,Fig.
R12758	T15856	T15849	punct	),failed
R12759	T15857	T15839	punct	.,reduced
R12764	T15862	T15863	auxpass	is,required
R12765	T15863	T15863	ROOT	required,required
R12766	T15864	T15865	aux	to,protect
R12767	T15865	T15863	xcomp	protect,required
R12768	T15866	T15865	dobj	RPS3,protect
R12769	T15867	T15865	prep	from,protect
R12770	T15868	T15869	amod	IKKβ-mediated,phosphorylation
R12771	T15869	T15867	pobj	phosphorylation,from
R12772	T15870	T15863	punct	.,required
R12773	T15871	T15872	compound	Citrobacter,rodentium
R12774	T15872	T15873	nsubj	rodentium,is
R12775	T15873	T15895	ccomp	is,inhibited
R12776	T15874	T15876	det	a,pathogen
R12777	T15875	T15876	compound	mouse,pathogen
R12778	T15876	T15873	attr	pathogen,is
R12779	T15877	T15878	nsubj	that,shares
R12780	T15878	T15876	relcl	shares,pathogen
R12781	T15879	T15880	amod	pathogenic,strategies
R12782	T15880	T15878	dobj	strategies,shares
R12783	T15881	T15880	prep	with,strategies
R12784	T15882	T15883	compound	E.,coli
R12785	T15883	T15881	pobj	coli,with
R12786	T15884	T15883	appos	39,coli
R12787	T15885	T15883	punct	",",coli
R12788	T15886	T15887	advmod	most,notably
R12789	T15887	T15888	advmod	notably,for
R12790	T15888	T15878	prep	for,shares
R12791	T15889	T15890	poss	our,investigation
R12792	T15890	T15888	pobj	investigation,for
R12793	T15891	T15895	punct	",",inhibited
R12794	T15892	T15894	compound	C.,NleH
R12795	T15893	T15894	compound	rodentium,NleH
R12796	T15894	T15895	nsubj	NleH,inhibited
R12797	T15895	T15895	ROOT	inhibited,inhibited
R12798	T15896	T15898	nummod	RPS3,translocation
R12799	T15897	T15898	amod	nuclear,translocation
R12800	T15898	T15895	dobj	translocation,inhibited
R12801	T15899	T15898	cc	and,translocation
R12802	T15900	T15903	amod	RPS3-dependent,activity
R12803	T15901	T15903	compound	NF-κB,activity
R12804	T15902	T15903	compound	luciferase,activity
R12805	T15903	T15898	conj	activity,translocation
R12806	T15904	T15895	prep	to,inhibited
R12807	T15905	T15906	det	an,extent
R12808	T15906	T15904	pobj	extent,to
R12809	T15907	T15906	amod	equivalent,extent
R12810	T15908	T15907	prep	to,equivalent
R12811	T15909	T15911	compound	E.,NleH1
R12812	T15910	T15911	compound	coli,NleH1
R12813	T15911	T15908	pobj	NleH1,to
R12814	T15912	T15911	punct	(,NleH1
R12815	T15913	T15911	appos	ref,NleH1
R12816	T15914	T15915	punct	.,9
R12817	T15915	T15915	ROOT	9,9
R12818	T15916	T15915	punct	),9
R12819	T15917	T15895	punct	.,inhibited
R12820	T15918	T15919	nsubj	We,assayed
R12821	T15919	T15919	ROOT	assayed,assayed
R12822	T15920	T15922	compound	RPS3,phosphorylation
R12823	T15921	T15922	compound	S209,phosphorylation
R12824	T15922	T15919	dobj	phosphorylation,assayed
R12825	T15923	T15919	prep	in,assayed
R12826	T15924	T15925	compound	HeLa,cells
R12827	T15925	T15923	pobj	cells,in
R12828	T15926	T15925	acl	infected,cells
R12829	T15927	T15926	prep	with,infected
R12830	T15928	T15931	amod	different,strains
R12831	T15929	T15931	nmod	C.,strains
R12832	T15930	T15931	compound	rodentium,strains
R12833	T15931	T15927	pobj	strains,with
R12834	T15932	T15919	punct	.,assayed
R12835	T15933	T15938	prep	In,stimulated
R12836	T15934	T15935	amod	uninfected,cells
R12837	T15935	T15933	pobj	cells,In
R12838	T15936	T15938	punct	",",stimulated
R12839	T15937	T15938	nsubj	TNF-treatment,stimulated
R12840	T15938	T15938	ROOT	stimulated,stimulated
R12841	T15939	T15942	det	a,increase
R12842	T15940	T15942	nmod	∼,increase
R12843	T15941	T15942	amod	3.5-fold,increase
R12844	T15942	T15938	dobj	increase,stimulated
R12845	T15943	T15942	prep	in,increase
R12846	T15944	T15946	compound	RPS3,phosphorylation
R12847	T15945	T15946	compound	S209,phosphorylation
R12848	T15946	T15943	pobj	phosphorylation,in
R12849	T15947	T15948	punct	(,Fig.
R12850	T15948	T15942	appos	Fig.,increase
R12851	T15949	T15948	nummod	7c,Fig.
R12852	T15950	T15948	punct	),Fig.
R12853	T15951	T15938	punct	.,stimulated
R12854	T15952	T15953	amod	Such,augmentation
R12855	T15953	T15958	nsubjpass	augmentation,reduced
R12856	T15954	T15953	prep	of,augmentation
R12857	T15955	T15956	nummod	RPS3,phosphorylation
R12858	T15956	T15954	pobj	phosphorylation,of
R12859	T15957	T15958	auxpass	was,reduced
R12860	T15958	T15958	ROOT	reduced,reduced
R12861	T15959	T15958	prep	by,reduced
R12862	T15960	T15961	advmod	about,60
R12863	T15961	T15962	nummod	60,%
R12864	T15962	T15959	pobj	%,by
R12865	T15963	T15958	agent	by,reduced
R12866	T15964	T15967	amod	wild-type,infection
R12867	T15965	T15966	compound	C.,rodentium
R12868	T15966	T15967	compound	rodentium,infection
R12869	T15967	T15963	pobj	infection,by
R12870	T15968	T15969	punct	(,Fig.
R12871	T15969	T15970	compound	Fig.,7c
R12872	T15970	T15967	appos	7c,infection
R12873	T15971	T15967	punct	),infection
R12874	T15972	T15958	punct	.,reduced
R12875	T15973	T15978	advmod	However,enhanced
R12876	T15974	T15978	punct	",",enhanced
R12877	T15975	T15976	amod	RPS3,phosphorylation
R12878	T15976	T15978	nsubjpass	phosphorylation,enhanced
R12879	T15977	T15978	auxpass	was,enhanced
R12880	T15978	T15978	ROOT	enhanced,enhanced
R12881	T15979	T15982	advmod	when,infected
R12882	T15980	T15982	nsubjpass	cells,infected
R12883	T15981	T15982	auxpass	were,infected
R12884	T15982	T15978	advcl	infected,enhanced
R12885	T15983	T15982	prep	with,infected
R12886	T15984	T15987	det	a,strain
R12887	T15985	T15987	compound	C.,strain
R12888	T15986	T15987	compound	rodentium,strain
R12889	T15987	T15983	pobj	strain,with
R12890	T15988	T15987	acl	lacking,strain
R12891	T15989	T15988	dobj	NleH,lacking
R12892	T15990	T15989	punct	(,NleH
R12893	T15991	T15992	compound	Fig.,7c
R12894	T15992	T15989	appos	7c,NleH
R12895	T15993	T15989	punct	",",NleH
R12896	T15994	T15989	appos	ΔnleH,NleH
R12897	T15995	T15989	punct	),NleH
R12898	T15996	T15978	punct	.,enhanced
R12899	T15997	T15999	nsubj	We,examined
R12900	T15998	T15999	advmod	further,examined
R12901	T15999	T15999	ROOT	examined,examined
R12902	T16000	T16001	det	the,role
R12903	T16001	T15999	dobj	role,examined
R12904	T16002	T16001	prep	of,role
R12905	T16003	T16005	amod	NleH1,activity
R12906	T16004	T16005	compound	kinase,activity
R12907	T16005	T16002	pobj	activity,of
R12908	T16006	T15999	advcl	using,examined
R12909	T16007	T16011	det	the,strain
R12910	T16008	T16010	compound	C.,ΔnleH
R12911	T16009	T16010	compound	rodentium,ΔnleH
R12912	T16010	T16011	compound	ΔnleH,strain
R12913	T16011	T16006	dobj	strain,using
R12914	T16012	T16006	prep	as,using
R12915	T16013	T16014	det	a,background
R12916	T16014	T16012	pobj	background,as
R12917	T16015	T16018	prep	on,express
R12918	T16016	T16015	pobj	which,on
R12919	T16017	T16018	aux	to,express
R12920	T16018	T16014	relcl	express,background
R12921	T16019	T16020	preconj	either,wild-type
R12922	T16020	T16018	dobj	wild-type,express
R12923	T16021	T16020	cc	or,wild-type
R12924	T16022	T16025	compound	K159A,NleH1
R12925	T16023	T16025	compound	E.,NleH1
R12926	T16024	T16025	compound	coli,NleH1
R12927	T16025	T16020	conj	NleH1,wild-type
R12928	T16026	T15999	punct	.,examined
R12929	T16027	T16027	ROOT	Complementing,Complementing
R12930	T16028	T16034	nsubj	ΔnleH,abolished
R12931	T16029	T16028	amod	mutant,ΔnleH
R12932	T16030	T16029	prep	with,mutant
R12933	T16031	T16032	compound	wild-type,NleH1
R12934	T16032	T16030	pobj	NleH1,with
R12935	T16033	T16034	advmod	almost,abolished
R12936	T16034	T16034	ROOT	abolished,abolished
R12937	T16035	T16038	amod	TNF-induced,phosphorylation
R12938	T16036	T16037	compound	RPS3,S209
R12939	T16037	T16038	compound	S209,phosphorylation
R12940	T16038	T16034	dobj	phosphorylation,abolished
R12941	T16039	T16043	mark	whereas,failed
R12942	T16040	T16039	pcomp	complementing,whereas
R12943	T16041	T16040	prep	with,complementing
R12944	T16042	T16041	pobj	K159A,with
R12945	T16043	T16034	advcl	failed,abolished
R12946	T16044	T16045	aux	to,inhibit
R12947	T16045	T16043	xcomp	inhibit,failed
R12948	T16046	T16047	amod	RPS3,phosphorylation
R12949	T16047	T16045	dobj	phosphorylation,inhibit
R12950	T16048	T16049	punct	(,Fig.
R12951	T16049	T16047	appos	Fig.,phosphorylation
R12952	T16050	T16049	nummod	7c,Fig.
R12953	T16051	T16049	punct	),Fig.
R12954	T16052	T16034	punct	.,abolished
R12955	T16053	T16057	advmod	Collectively,demonstrate
R12956	T16054	T16057	punct	",",demonstrate
R12957	T16055	T16056	det	these,results
R12958	T16056	T16057	nsubj	results,demonstrate
R12959	T16057	T16057	ROOT	demonstrate,demonstrate
R12960	T16058	T16063	mark	that,required
R12961	T16059	T16061	amod	NleH1,activity
R12962	T16060	T16061	compound	kinase,activity
R12963	T16061	T16063	nsubjpass	activity,required
R12964	T16062	T16063	auxpass	is,required
R12965	T16063	T16057	ccomp	required,demonstrate
R12966	T16064	T16065	aux	to,block
R12967	T16065	T16063	xcomp	block,required
R12968	T16066	T16068	compound	RPS3,phosphorylation
R12969	T16067	T16068	compound	S209,phosphorylation
R12970	T16068	T16065	dobj	phosphorylation,block
R12971	T16069	T16057	punct	.,demonstrate
R12972	T16070	T16072	nsubj	We,examined
R12973	T16071	T16072	advmod	next,examined
R12974	T16072	T16072	ROOT	examined,examined
R12975	T16073	T16079	mark	whether,is
R12976	T16074	T16076	det	the,activity
R12977	T16075	T16076	amod	inhibitory,activity
R12978	T16076	T16079	nsubj	activity,is
R12979	T16077	T16076	prep	of,activity
R12980	T16078	T16077	pobj	NleH1,of
R12981	T16079	T16072	ccomp	is,examined
R12982	T16080	T16081	advmod	sufficiently,robust
R12983	T16081	T16079	acomp	robust,is
R12984	T16082	T16083	aux	to,impair
R12985	T16083	T16079	xcomp	impair,is
R12986	T16084	T16087	det	the,translocation
R12987	T16085	T16087	amod	strong,translocation
R12988	T16086	T16087	amod	nuclear,translocation
R12989	T16087	T16083	dobj	translocation,impair
R12990	T16088	T16087	prep	of,translocation
R12991	T16089	T16088	pobj	RPS3,of
R12992	T16090	T16087	acl	trigged,translocation
R12993	T16091	T16090	agent	by,trigged
R12994	T16092	T16094	det	the,IKKβ
R12995	T16093	T16094	compound	constitutively-active,IKKβ
R12996	T16094	T16091	pobj	IKKβ,by
R12997	T16095	T16099	punct	(,]
R12998	T16096	T16099	compound	IKKβ,]
R12999	T16097	T16099	compound	[,]
R13000	T16098	T16099	compound	SSEE,]
R13001	T16099	T16094	appos	],IKKβ
R13002	T16100	T16099	punct	),]
R13003	T16101	T16102	punct	(,Fig.
R13004	T16102	T16094	appos	Fig.,IKKβ
R13005	T16103	T16102	nummod	2d,Fig.
R13006	T16104	T16094	punct	),IKKβ
R13007	T16105	T16072	punct	.,examined
R13008	T16106	T16107	nsubj	We,found
R13009	T16107	T16107	ROOT	found,found
R13010	T16108	T16118	mark	that,triggered
R13011	T16109	T16110	advmod	ectopically,expressing
R13012	T16110	T16118	advcl	expressing,triggered
R13013	T16111	T16112	preconj	either,wild-type
R13014	T16112	T16110	dobj	wild-type,expressing
R13015	T16113	T16112	cc	or,wild-type
R13016	T16114	T16115	compound	SSEE,IKKβ
R13017	T16115	T16112	conj	IKKβ,wild-type
R13018	T16116	T16112	conj	proteins,wild-type
R13019	T16117	T16118	punct	",",triggered
R13020	T16118	T16107	ccomp	triggered,found
R13021	T16119	T16120	advmod	more,RPS3
R13022	T16120	T16122	amod	RPS3,translocation
R13023	T16121	T16122	amod	nuclear,translocation
R13024	T16122	T16118	dobj	translocation,triggered
R13025	T16123	T16122	prep	than,translocation
R13026	T16124	T16126	det	the,IKKβ
R13027	T16125	T16126	compound	kinase-dead,IKKβ
R13028	T16126	T16130	nmod	IKKβ,protein
R13029	T16127	T16126	punct	(,IKKβ
R13030	T16128	T16126	appos	SSAA,IKKβ
R13031	T16129	T16126	punct	),IKKβ
R13032	T16130	T16123	pobj	protein,than
R13033	T16131	T16130	punct	(,protein
R13034	T16132	T16130	appos	Fig.,protein
R13035	T16133	T16132	nummod	7d,Fig.
R13036	T16134	T16130	punct	),protein
R13037	T16135	T16107	punct	.,found
R13038	T16136	T16138	nummod	RPS3,accumulation
R13039	T16137	T16138	amod	nuclear,accumulation
R13040	T16138	T16139	nsubj	accumulation,was
R13041	T16139	T16139	ROOT	was,was
R13042	T16140	T16141	advmod	substantially,retarded
R13043	T16141	T16139	acomp	retarded,was
R13044	T16142	T16139	prep	by,was
R13045	T16143	T16142	pcomp	infecting,by
R13046	T16144	T16143	dobj	cells,infecting
R13047	T16145	T16143	prep	with,infecting
R13048	T16146	T16149	amod	wild-type,O157
R13049	T16147	T16149	compound	E.,O157
R13050	T16148	T16149	compound	coli,O157
R13051	T16149	T16145	pobj	O157,with
R13052	T16150	T16149	punct	:,O157
R13053	T16151	T16149	appos	H7,O157
R13054	T16152	T16153	punct	(,Fig.
R13055	T16153	T16151	appos	Fig.,H7
R13056	T16154	T16153	nummod	7d,Fig.
R13057	T16155	T16151	punct	),H7
R13058	T16156	T16139	punct	.,was
R13059	T16157	T16160	prep	In,infecting
R13060	T16158	T16157	pobj	contrast,In
R13061	T16159	T16160	punct	",",infecting
R13062	T16160	T16160	ROOT	infecting,infecting
R13063	T16161	T16160	prep	with,infecting
R13064	T16162	T16163	det	either,ΔnleH1
R13065	T16163	T16161	pobj	ΔnleH1,with
R13066	T16164	T16163	cc	or,ΔnleH1
R13067	T16165	T16163	conj	ΔescN,ΔnleH1
R13068	T16166	T16160	dobj	strains,infecting
R13069	T16167	T16168	advmod	only,slightly
R13070	T16168	T16169	advmod	slightly,impaired
R13071	T16169	T16166	acl	impaired,strains
R13072	T16170	T16172	nmod	RPS3,translocation
R13073	T16171	T16172	amod	nuclear,translocation
R13074	T16172	T16169	dobj	translocation,impaired
R13075	T16173	T16169	prep	in,impaired
R13076	T16174	T16175	preconj	either,IKKβ
R13077	T16175	T16173	pobj	IKKβ,in
R13078	T16176	T16175	punct	-,IKKβ
R13079	T16177	T16175	cc	or,IKKβ
R13080	T16178	T16175	conj	IKKβ,IKKβ
R13081	T16179	T16178	punct	(,IKKβ
R13082	T16180	T16178	appos	SSEE,IKKβ
R13083	T16181	T16178	punct	),IKKβ
R13084	T16182	T16183	punct	-,expressing
R13085	T16183	T16166	acl	expressing,strains
R13086	T16184	T16183	dobj	cells,expressing
R13087	T16185	T16183	punct	(,expressing
R13088	T16186	T16188	nmod	Fig.,)
R13089	T16187	T16186	nummod	7d,Fig.
R13090	T16188	T16183	punct	),expressing
R13091	T16189	T16160	punct	.,infecting
R13092	T16190	T16191	mark	As,expected
R13093	T16191	T16198	advcl	expected,affect
R13094	T16192	T16198	punct	",",affect
R13095	T16193	T16195	compound	E.,infections
R13096	T16194	T16195	compound	coli,infections
R13097	T16195	T16198	nsubj	infections,affect
R13098	T16196	T16198	aux	did,affect
R13099	T16197	T16198	neg	not,affect
R13100	T16198	T16198	ROOT	affect,affect
R13101	T16199	T16202	det	the,translocation
R13102	T16200	T16202	amod	RPS3,translocation
R13103	T16201	T16202	amod	nuclear,translocation
R13104	T16202	T16198	dobj	translocation,affect
R13105	T16203	T16202	prep	in,translocation
R13106	T16204	T16203	pobj	IKKβ,in
R13107	T16205	T16204	punct	(,IKKβ
R13108	T16206	T16204	appos	SSAA,IKKβ
R13109	T16207	T16204	punct	),IKKβ
R13110	T16208	T16202	punct	-,translocation
R13111	T16209	T16202	acl	expressing,translocation
R13112	T16210	T16209	dobj	cells,expressing
R13113	T16211	T16209	punct	",",expressing
R13114	T16212	T16215	advmod	where,was
R13115	T16213	T16214	amod	NF-κB,signaling
R13116	T16214	T16215	nsubj	signaling,was
R13117	T16215	T16202	relcl	was,translocation
R13118	T16216	T16215	acomp	low,was
R13119	T16217	T16218	punct	(,Fig.
R13120	T16218	T16202	appos	Fig.,translocation
R13121	T16219	T16218	nummod	7d,Fig.
R13122	T16220	T16218	punct	),Fig.
R13123	T16221	T16198	punct	.,affect
R13124	T16222	T16227	advmod	Thus,is
R13125	T16223	T16227	punct	",",is
R13126	T16224	T16227	prep	during,is
R13127	T16225	T16224	pobj	infection,during
R13128	T16226	T16227	nsubj	NleH1,is
R13129	T16227	T16227	ROOT	is,is
R13130	T16228	T16229	advmod	sufficiently,potent
R13131	T16229	T16227	acomp	potent,is
R13132	T16230	T16231	aux	to,inhibit
R13133	T16231	T16229	xcomp	inhibit,potent
R13134	T16232	T16234	amod	RPS3,translocation
R13135	T16233	T16234	amod	nuclear,translocation
R13136	T16234	T16231	dobj	translocation,inhibit
R13137	T16235	T16236	advmod	even,in
R13138	T16236	T16231	prep	in,inhibit
R13139	T16237	T16236	pobj	cells,in
R13140	T16238	T16237	acl	expressing,cells
R13141	T16239	T16240	compound	constitutively-activated,IKKβ
R13142	T16240	T16238	dobj	IKKβ,expressing
R13143	T16241	T16227	punct	.,is
R13144	T16242	T16243	nsubj	We,examined
R13145	T16243	T16243	ROOT	examined,examined
R13146	T16244	T16248	mark	whether,phosphorylate
R13147	T16245	T16248	nsubj	NleH1,phosphorylate
R13148	T16246	T16248	aux	could,phosphorylate
R13149	T16247	T16248	advmod	directly,phosphorylate
R13150	T16248	T16243	ccomp	phosphorylate,examined
R13151	T16249	T16248	dobj	IKKβ,phosphorylate
R13152	T16250	T16251	advmod	thus,inhibiting
R13153	T16251	T16248	advcl	inhibiting,phosphorylate
R13154	T16252	T16255	amod	IKKβ-mediated,phosphorylation
R13155	T16253	T16255	nummod	RPS3,phosphorylation
R13156	T16254	T16255	nummod	S209,phosphorylation
R13157	T16255	T16251	dobj	phosphorylation,inhibiting
R13158	T16256	T16243	punct	.,examined
R13159	T16257	T16258	nsubj	We,performed
R13160	T16258	T16258	ROOT	performed,performed
R13161	T16259	T16258	prep	in,performed
R13162	T16260	T16261	amod	vitro,kinase
R13163	T16261	T16262	compound	kinase,assays
R13164	T16262	T16259	pobj	assays,in
R13165	T16263	T16262	advcl	using,assays
R13166	T16264	T16265	amod	immunoprecipitated,Flag-IKKβ
R13167	T16265	T16263	dobj	Flag-IKKβ,using
R13168	T16266	T16267	punct	(,K44A
R13169	T16267	T16265	appos	K44A,Flag-IKKβ
R13170	T16268	T16265	punct	),Flag-IKKβ
R13171	T16269	T16263	prep	as,using
R13172	T16270	T16269	pobj	substrate,as
R13173	T16271	T16270	cc	and,substrate
R13174	T16272	T16273	amod	recombinant,His-NleH1
R13175	T16273	T16270	conj	His-NleH1,substrate
R13176	T16274	T16263	prep	as,using
R13177	T16275	T16274	pobj	kinase,as
R13178	T16276	T16262	punct	",",assays
R13179	T16277	T16283	mark	so,obscure
R13180	T16278	T16283	mark	that,obscure
R13181	T16279	T16280	compound	IKKβ,autophosphorylation
R13182	T16280	T16283	nsubj	autophosphorylation,obscure
R13183	T16281	T16283	aux	would,obscure
R13184	T16282	T16283	neg	not,obscure
R13185	T16283	T16258	advcl	obscure,performed
R13186	T16284	T16285	amod	NleH1-induced,phosphorylation
R13187	T16285	T16283	dobj	phosphorylation,obscure
R13188	T16286	T16258	punct	.,performed
R13189	T16287	T16292	advmod	However,observe
R13190	T16288	T16292	punct	",",observe
R13191	T16289	T16292	nsubj	we,observe
R13192	T16290	T16292	aux	did,observe
R13193	T16291	T16292	neg	not,observe
R13194	T16292	T16292	ROOT	observe,observe
R13195	T16293	T16296	det	any,incorporation
R13196	T16294	T16296	amod	detectable,incorporation
R13197	T16295	T16296	compound	32P,incorporation
R13198	T16296	T16292	dobj	incorporation,observe
R13199	T16297	T16296	prep	in,incorporation
R13200	T16298	T16297	pobj	IKKβ,in
R13201	T16299	T16301	punct	(,Fig.
R13202	T16300	T16301	compound	Supplementary,Fig.
R13203	T16301	T16298	appos	Fig.,IKKβ
R13204	T16302	T16301	nummod	13,Fig.
R13205	T16303	T16301	punct	),Fig.
R13206	T16304	T16292	punct	",",observe
R13207	T16305	T16306	advmod	thus,ruling
R13208	T16306	T16292	advcl	ruling,observe
R13209	T16307	T16306	prt	out,ruling
R13210	T16308	T16309	det	this,possibility
R13211	T16309	T16306	dobj	possibility,ruling
R13212	T16310	T16292	punct	.,observe
R13213	T16311	T16313	nsubj	We,tested
R13214	T16312	T16313	advmod	then,tested
R13215	T16313	T16313	ROOT	tested,tested
R13216	T16314	T16315	det	the,hypothesis
R13217	T16315	T16313	dobj	hypothesis,tested
R13218	T16316	T16319	mark	that,alter
R13219	T16317	T16319	nsubj	NleH1,alter
R13220	T16318	T16319	aux	could,alter
R13221	T16319	T16315	acl	alter,hypothesis
R13222	T16320	T16322	det	the,substrate
R13223	T16321	T16322	compound	IKKβ,substrate
R13224	T16322	T16323	compound	substrate,specificities
R13225	T16323	T16319	dobj	specificities,alter
R13226	T16324	T16313	punct	.,tested
R13227	T16325	T16330	prep	To,performed
R13228	T16326	T16327	det	this,end
R13229	T16327	T16325	pobj	end,To
R13230	T16328	T16330	punct	",",performed
R13231	T16329	T16330	nsubj	we,performed
R13232	T16330	T16330	ROOT	performed,performed
R13233	T16331	T16330	prep	in,performed
R13234	T16332	T16333	amod	vitro,kinase
R13235	T16333	T16334	compound	kinase,assays
R13236	T16334	T16331	pobj	assays,in
R13237	T16335	T16334	advcl	using,assays
R13238	T16336	T16337	det	both,CK2
R13239	T16337	T16335	dobj	CK2,using
R13240	T16338	T16337	cc	and,CK2
R13241	T16339	T16340	compound	IKK,substrates
R13242	T16340	T16337	conj	substrates,CK2
R13243	T16341	T16340	prep	for,substrates
R13244	T16342	T16341	pobj	IKKβ,for
R13245	T16343	T16330	punct	.,performed
R13246	T16344	T16345	mark	As,expected
R13247	T16345	T16348	advcl	expected,phosphorylated
R13248	T16346	T16348	punct	",",phosphorylated
R13249	T16347	T16348	nsubj	IKKβ,phosphorylated
R13250	T16348	T16348	ROOT	phosphorylated,phosphorylated
R13251	T16349	T16348	dobj	RPS3,phosphorylated
R13252	T16350	T16351	punct	(,Fig.
R13253	T16351	T16352	compound	Fig.,7e
R13254	T16352	T16349	appos	7e,RPS3
R13255	T16353	T16352	punct	",",7e
R13256	T16354	T16352	appos	lane,7e
R13257	T16355	T16354	nummod	7,lane
R13258	T16356	T16352	punct	),7e
R13259	T16357	T16349	cc	and,RPS3
R13260	T16358	T16349	conj	GST-IκBα,RPS3
R13261	T16359	T16358	punct	(,GST-IκBα
R13262	T16360	T16358	parataxis	1-54,GST-IκBα
R13263	T16361	T16358	punct	),GST-IκBα
R13264	T16362	T16349	conj	protein,RPS3
R13265	T16363	T16362	punct	(,protein
R13266	T16364	T16365	compound	Fig.,7e
R13267	T16365	T16362	appos	7e,protein
R13268	T16366	T16365	punct	",",7e
R13269	T16367	T16368	nmod	lane,9
R13270	T16368	T16365	conj	9,7e
R13271	T16369	T16365	punct	),7e
R13272	T16370	T16362	punct	",",protein
R13273	T16371	T16348	advcl	demonstrating,phosphorylated
R13274	T16372	T16373	nsubj	it,harbors
R13275	T16373	T16371	ccomp	harbors,demonstrating
R13276	T16374	T16375	preconj	either,CK2
R13277	T16375	T16373	dobj	CK2,harbors
R13278	T16376	T16375	cc	or,CK2
R13279	T16377	T16379	compound	IKK,specificity
R13280	T16378	T16379	compound	substrate,specificity
R13281	T16379	T16375	conj	specificity,CK2
R13282	T16380	T16348	punct	.,phosphorylated
R13283	T16381	T16386	nsubj	Preincubation,reduced
R13284	T16382	T16381	prep	of,Preincubation
R13285	T16383	T16382	pobj	IKKβ,of
R13286	T16384	T16381	prep	with,Preincubation
R13287	T16385	T16384	pobj	NleH1,with
R13288	T16386	T16386	ROOT	reduced,reduced
R13289	T16387	T16389	nmod	IKKβ-mediated,phosphorylation
R13290	T16388	T16389	amod	RPS3,phosphorylation
R13291	T16389	T16386	dobj	phosphorylation,reduced
R13292	T16390	T16389	punct	",",phosphorylation
R13293	T16391	T16395	advmod	i.e.,specificity
R13294	T16392	T16395	det	the,specificity
R13295	T16393	T16395	compound	CK2,specificity
R13296	T16394	T16395	compound	kinase,specificity
R13297	T16395	T16389	appos	specificity,phosphorylation
R13298	T16396	T16395	punct	",",specificity
R13299	T16397	T16395	cc	but,specificity
R13300	T16398	T16399	neg	not,IKKβ-mediated
R13301	T16399	T16401	amod	IKKβ-mediated,phosphorylation
R13302	T16400	T16401	amod	GST-IκBα,phosphorylation
R13303	T16401	T16395	conj	phosphorylation,specificity
R13304	T16402	T16401	punct	",",phosphorylation
R13305	T16403	T16407	advmod	i.e.,specificity
R13306	T16404	T16407	det	the,specificity
R13307	T16405	T16407	nmod	IKK,specificity
R13308	T16406	T16407	compound	kinase,specificity
R13309	T16407	T16401	appos	specificity,phosphorylation
R13310	T16408	T16407	punct	(,specificity
R13311	T16409	T16407	appos	Fig.,specificity
R13312	T16410	T16409	nummod	7e,Fig.
R13313	T16411	T16407	punct	),specificity
R13314	T16412	T16386	punct	.,reduced
R13315	T16413	T16414	compound	Control,experiments
R13316	T16414	T16415	nsubj	experiments,revealed
R13317	T16415	T16415	ROOT	revealed,revealed
R13318	T16416	T16418	det	no,phosphorylation
R13319	T16417	T16418	amod	NleH1-mediated,phosphorylation
R13320	T16418	T16415	dobj	phosphorylation,revealed
R13321	T16419	T16418	cc	or,phosphorylation
R13322	T16420	T16418	conj	autophosphorylation,phosphorylation
R13323	T16421	T16418	prep	of,phosphorylation
R13324	T16422	T16421	pobj	RPS3,of
R13325	T16423	T16422	cc	or,RPS3
R13326	T16424	T16422	conj	GST-IκBα,RPS3
R13327	T16425	T16426	punct	(,Fig.
R13328	T16426	T16424	appos	Fig.,GST-IκBα
R13329	T16427	T16426	nummod	7e,Fig.
R13330	T16428	T16424	punct	),GST-IκBα
R13331	T16429	T16415	punct	.,revealed
R13332	T16430	T16434	advcl	Taken,blocks
R13333	T16431	T16430	advmod	together,Taken
R13334	T16432	T16434	punct	",",blocks
R13335	T16433	T16434	nsubj	NleH1,blocks
R13336	T16434	T16434	ROOT	blocks,blocks
R13337	T16435	T16438	det	the,specificity
R13338	T16436	T16438	compound	CK2,specificity
R13339	T16437	T16438	compound	substrate,specificity
R13340	T16438	T16434	dobj	specificity,blocks
R13341	T16439	T16438	prep	of,specificity
R13342	T16440	T16439	pobj	IKKβ,of
R13343	T16441	T16442	advmod	thus,inhibiting
R13344	T16442	T16438	acl	inhibiting,specificity
R13345	T16443	T16447	det	the,phosphorylation
R13346	T16444	T16447	amod	IKKβ-mediated,phosphorylation
R13347	T16445	T16446	nummod	RPS3,S209
R13348	T16446	T16447	nummod	S209,phosphorylation
R13349	T16447	T16442	dobj	phosphorylation,inhibiting
R13350	T16448	T16449	advmod	thus,representing
R13351	T16449	T16438	acl	representing,specificity
R13352	T16450	T16452	det	a,strategy
R13353	T16451	T16452	compound	novel,strategy
R13354	T16452	T16449	dobj	strategy,representing
R13355	T16453	T16452	prep	by,strategy
R13356	T16454	T16456	compound	E.,O157
R13357	T16455	T16456	compound	coli,O157
R13358	T16456	T16453	pobj	O157,by
R13359	T16457	T16438	punct	:,specificity
R13360	T16458	T16438	appos	H7,specificity
R13361	T16459	T16460	aux	to,alter
R13362	T16460	T16438	acl	alter,specificity
R13363	T16461	T16462	det	the,host
R13364	T16462	T16465	amod	host,response
R13365	T16463	T16465	amod	innate,response
R13366	T16464	T16465	amod	immune,response
R13367	T16465	T16460	dobj	response,alter
R13368	T16466	T16434	punct	.,blocks
R12760	T15858	T15863	advmod	Thus,required
R12761	T15859	T15861	amod	NleH1,activity
R12762	T15860	T15861	compound	kinase,activity
R12763	T15861	T15863	nsubjpass	activity,required

GO-BP

Id	Subject	Object	Predicate	Lexical cue
T16692	202-217	http://purl.obolibrary.org/obo/GO_0016310	denotes	phosphorylation
T16693	699-714	http://purl.obolibrary.org/obo/GO_0016310	denotes	phosphorylation
T16694	842-857	http://purl.obolibrary.org/obo/GO_0016310	denotes	phosphorylation
T16695	1160-1175	http://purl.obolibrary.org/obo/GO_0016310	denotes	phosphorylation
T16696	1316-1331	http://purl.obolibrary.org/obo/GO_0016310	denotes	phosphorylation
T16697	1369-1384	http://purl.obolibrary.org/obo/GO_0016310	denotes	phosphorylation
T16698	1471-1486	http://purl.obolibrary.org/obo/GO_0016310	denotes	phosphorylation
T16699	1837-1852	http://purl.obolibrary.org/obo/GO_0016310	denotes	phosphorylation
T16700	1909-1924	http://purl.obolibrary.org/obo/GO_0016310	denotes	phosphorylation
T16701	2034-2049	http://purl.obolibrary.org/obo/GO_0016310	denotes	phosphorylation
T16702	3101-3116	http://purl.obolibrary.org/obo/GO_0016310	denotes	phosphorylation
T16703	3311-3326	http://purl.obolibrary.org/obo/GO_0016310	denotes	phosphorylation
T16704	3865-3880	http://purl.obolibrary.org/obo/GO_0016310	denotes	phosphorylation
T16705	3946-3961	http://purl.obolibrary.org/obo/GO_0016310	denotes	phosphorylation
T16706	4053-4068	http://purl.obolibrary.org/obo/GO_0016310	denotes	phosphorylation
T16707	4234-4249	http://purl.obolibrary.org/obo/GO_0016310	denotes	phosphorylation
T16708	468-483	http://purl.obolibrary.org/obo/GO_0016301	denotes	kinase activity
T16709	779-794	http://purl.obolibrary.org/obo/GO_0016301	denotes	kinase activity
T16710	1621-1636	http://purl.obolibrary.org/obo/GO_0016301	denotes	kinase activity
T16711	1987-2002	http://purl.obolibrary.org/obo/GO_0016301	denotes	kinase activity
T16712	1068-1087	http://purl.obolibrary.org/obo/GO_0045289	denotes	luciferase activity
T16713	1068-1087	http://purl.obolibrary.org/obo/GO_0047077	denotes	luciferase activity
T16714	1068-1087	http://purl.obolibrary.org/obo/GO_0047712	denotes	luciferase activity
T16715	1068-1087	http://purl.obolibrary.org/obo/GO_0050248	denotes	luciferase activity
T16716	1068-1087	http://purl.obolibrary.org/obo/GO_0050397	denotes	luciferase activity
T16717	2820-2835	http://purl.obolibrary.org/obo/GO_0038061	denotes	NF-κB signaling
T16718	2826-2835	http://purl.obolibrary.org/obo/GO_0023052	denotes	signaling
T16719	3611-3614	http://purl.obolibrary.org/obo/GO_0008384	denotes	IKK
T16720	3778-3781	http://purl.obolibrary.org/obo/GO_0008384	denotes	IKK
T16721	3972-3975	http://purl.obolibrary.org/obo/GO_0008384	denotes	IKK
T16722	4322-4344	http://purl.obolibrary.org/obo/GO_0045087	denotes	innate immune response
T16723	4322-4344	http://purl.obolibrary.org/obo/GO_0002227	denotes	innate immune response
T16724	4322-4344	http://purl.obolibrary.org/obo/GO_0045088	denotes	innate immune response
T16725	4322-4344	http://purl.obolibrary.org/obo/GO_0002218	denotes	innate immune response
T16726	4322-4344	http://purl.obolibrary.org/obo/GO_0045089	denotes	innate immune response
T16727	4322-4344	http://purl.obolibrary.org/obo/GO_0045824	denotes	innate immune response
T16728	4329-4344	http://purl.obolibrary.org/obo/GO_0006955	denotes	immune response

GO-MF

Id	Subject	Object	Predicate	Lexical cue
T16729	468-483	http://purl.obolibrary.org/obo/GO_0016301	denotes	kinase activity
T16730	779-794	http://purl.obolibrary.org/obo/GO_0016301	denotes	kinase activity
T16731	1621-1636	http://purl.obolibrary.org/obo/GO_0016301	denotes	kinase activity
T16732	1987-2002	http://purl.obolibrary.org/obo/GO_0016301	denotes	kinase activity
T16733	1068-1087	http://purl.obolibrary.org/obo/GO_0045289	denotes	luciferase activity
T16734	1068-1087	http://purl.obolibrary.org/obo/GO_0047077	denotes	luciferase activity
T16735	1068-1087	http://purl.obolibrary.org/obo/GO_0047712	denotes	luciferase activity
T16736	1068-1087	http://purl.obolibrary.org/obo/GO_0050248	denotes	luciferase activity
T16737	1068-1087	http://purl.obolibrary.org/obo/GO_0050397	denotes	luciferase activity
T16738	3611-3614	http://purl.obolibrary.org/obo/GO_0008384	denotes	IKK
T16739	3778-3781	http://purl.obolibrary.org/obo/GO_0008384	denotes	IKK
T16740	3972-3975	http://purl.obolibrary.org/obo/GO_0008384	denotes	IKK

GO-CC

Id	Subject	Object	Predicate	Lexical cue
T16741	621-626	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T16742	1184-1189	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T16743	1250-1255	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T16744	1505-1510	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T16745	2487-2492	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T16746	2686-2691	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T16747	2807-2812	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T16748	2953-2958	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T16749	4317-4321	http://purl.obolibrary.org/obo/GO_0018995	denotes	host

sentences

Id	Subject	Object	Predicate	Lexical cue
T14904	0-45	Sentence	denotes	NleH1 steers the IKKβ substrate specificities
T14905	46-142	Sentence	denotes	NleH1 is an autophosphorylated serine-threonine kinase, which depends on the lysine 159 (K159)9.
T14906	143-444	Sentence	denotes	To explore the mechanism by which NleH1 inhibits RPS3 S209 phosphorylation, we first performed an in vitro kinase assay with purified wild-type His-NleH1 protein and a mutant His-NleH1 (K159A) protein, confirming that NleH1 is autophosphorylated and the K159A is an NleH1 kinase-dead mutant (Fig. 7a).
T14907	445-627	Sentence	denotes	To examine whether the kinase activity is required for NleH1 to inhibit IKKβ phosphorlyation of RPS3 on S209, we ectopically expressing either wild-type or K159A NleH1 in 293T cells.
T14908	628-767	Sentence	denotes	Wild-type NleH1 expression significantly reduced TNF-induced RPS3 S209 phosphorylation, whereas the K159A mutant failed to do so (Fig. 7b).
T14909	768-858	Sentence	denotes	Thus NleH1 kinase activity is required to protect RPS3 from IKKβ-mediated phosphorylation.
T14910	859-1134	Sentence	denotes	Citrobacter rodentium is a mouse pathogen that shares pathogenic strategies with E. coli 39, most notably for our investigation, C. rodentium NleH inhibited RPS3 nuclear translocation and RPS3-dependent NF-κB luciferase activity to an extent equivalent to E. coli NleH1 (ref.
T14911	1135-1138	Sentence	denotes	9).
T14912	1139-1235	Sentence	denotes	We assayed RPS3 S209 phosphorylation in HeLa cells infected with different C. rodentium strains.
T14913	1236-1342	Sentence	denotes	In uninfected cells, TNF-treatment stimulated a ∼3.5-fold increase in RPS3 S209 phosphorylation (Fig. 7c).
T14914	1343-1456	Sentence	denotes	Such augmentation of RPS3 phosphorylation was reduced by about 60% by wild-type C. rodentium infection (Fig. 7c).
T14915	1457-1582	Sentence	denotes	However, RPS3 phosphorylation was enhanced when cells were infected with a C. rodentium strain lacking NleH (Fig. 7c, ΔnleH).
T14916	1583-1749	Sentence	denotes	We further examined the role of NleH1 kinase activity using the C. rodentium ΔnleH strain as a background on which to express either wild-type or K159A E. coli NleH1.
T14917	1750-1935	Sentence	denotes	Complementing ΔnleH mutant with wild-type NleH1 almost abolished TNF-induced RPS3 S209 phosphorylation whereas complementing with K159A failed to inhibit RPS3 phosphorylation (Fig. 7c).
T14918	1936-2050	Sentence	denotes	Collectively, these results demonstrate that NleH1 kinase activity is required to block RPS3 S209 phosphorylation.
T14919	2051-2249	Sentence	denotes	We next examined whether the inhibitory activity of NleH1 is sufficiently robust to impair the strong nuclear translocation of RPS3 trigged by the constitutively-active IKKβ (IKKβ [SSEE]) (Fig. 2d).
T14920	2250-2420	Sentence	denotes	We found that ectopically expressing either wild-type or SSEE IKKβ proteins, triggered more RPS3 nuclear translocation than the kinase-dead IKKβ (SSAA) protein (Fig. 7d).
T14921	2421-2534	Sentence	denotes	RPS3 nuclear accumulation was substantially retarded by infecting cells with wild-type E. coli O157:H7 (Fig. 7d).
T14922	2535-2702	Sentence	denotes	In contrast, infecting with either ΔnleH1 or ΔescN strains only slightly impaired RPS3 nuclear translocation in either IKKβ- or IKKβ (SSEE)-expressing cells (Fig. 7d).
T14923	2703-2854	Sentence	denotes	As expected, E. coli infections did not affect the RPS3 nuclear translocation in IKKβ (SSAA)-expressing cells, where NF-κB signaling was low (Fig. 7d).
T14924	2855-3000	Sentence	denotes	Thus, during infection NleH1 is sufficiently potent to inhibit RPS3 nuclear translocation even in cells expressing constitutively-activated IKKβ.
T14925	3001-3117	Sentence	denotes	We examined whether NleH1 could directly phosphorylate IKKβ thus inhibiting IKKβ-mediated RPS3 S209 phosphorylation.
T14926	3118-3327	Sentence	denotes	We performed in vitro kinase assays using immunoprecipitated Flag-IKKβ (K44A) as substrate and recombinant His-NleH1 as kinase, so that IKKβ autophosphorylation would not obscure NleH1-induced phosphorylation.
T14927	3328-3455	Sentence	denotes	However, we did not observe any detectable 32P incorporation in IKKβ (Supplementary Fig. 13), thus ruling out this possibility.
T14928	3456-3542	Sentence	denotes	We then tested the hypothesis that NleH1 could alter the IKKβ substrate specificities.
T14929	3543-3635	Sentence	denotes	To this end, we performed in vitro kinase assays using both CK2 and IKK substrates for IKKβ.
T14930	3636-3804	Sentence	denotes	As expected, IKKβ phosphorylated RPS3 (Fig. 7e, lane 7) and GST-IκBα (1-54) protein (Fig. 7e, lane 9), demonstrating it harbors either CK2 or IKK substrate specificity.
T14931	3805-4005	Sentence	denotes	Preincubation of IKKβ with NleH1 reduced IKKβ-mediated RPS3 phosphorylation, i.e. the CK2 kinase specificity, but not IKKβ-mediated GST-IκBα phosphorylation, i.e. the IKK kinase specificity (Fig. 7e).
T14932	4006-4122	Sentence	denotes	Control experiments revealed no NleH1-mediated phosphorylation or autophosphorylation of RPS3 or GST-IκBα (Fig. 7e).
T14933	4123-4345	Sentence	denotes	Taken together, NleH1 blocks the CK2 substrate specificity of IKKβ thus inhibiting the IKKβ-mediated RPS3 S209 phosphorylation thus representing a novel strategy by E. coli O157:H7 to alter the host innate immune response.
T134	0-45	Sentence	denotes	NleH1 steers the IKKβ substrate specificities
T135	46-142	Sentence	denotes	NleH1 is an autophosphorylated serine-threonine kinase, which depends on the lysine 159 (K159)9.
T136	143-444	Sentence	denotes	To explore the mechanism by which NleH1 inhibits RPS3 S209 phosphorylation, we first performed an in vitro kinase assay with purified wild-type His-NleH1 protein and a mutant His-NleH1 (K159A) protein, confirming that NleH1 is autophosphorylated and the K159A is an NleH1 kinase-dead mutant (Fig. 7a).
T137	445-627	Sentence	denotes	To examine whether the kinase activity is required for NleH1 to inhibit IKKβ phosphorlyation of RPS3 on S209, we ectopically expressing either wild-type or K159A NleH1 in 293T cells.
T138	628-767	Sentence	denotes	Wild-type NleH1 expression significantly reduced TNF-induced RPS3 S209 phosphorylation, whereas the K159A mutant failed to do so (Fig. 7b).
T139	768-858	Sentence	denotes	Thus NleH1 kinase activity is required to protect RPS3 from IKKβ-mediated phosphorylation.
T140	859-1134	Sentence	denotes	Citrobacter rodentium is a mouse pathogen that shares pathogenic strategies with E. coli 39, most notably for our investigation, C. rodentium NleH inhibited RPS3 nuclear translocation and RPS3-dependent NF-κB luciferase activity to an extent equivalent to E. coli NleH1 (ref.
T141	1135-1138	Sentence	denotes	9).
T142	1139-1235	Sentence	denotes	We assayed RPS3 S209 phosphorylation in HeLa cells infected with different C. rodentium strains.
T143	1236-1342	Sentence	denotes	In uninfected cells, TNF-treatment stimulated a ∼3.5-fold increase in RPS3 S209 phosphorylation (Fig. 7c).
T144	1343-1456	Sentence	denotes	Such augmentation of RPS3 phosphorylation was reduced by about 60% by wild-type C. rodentium infection (Fig. 7c).
T145	1457-1582	Sentence	denotes	However, RPS3 phosphorylation was enhanced when cells were infected with a C. rodentium strain lacking NleH (Fig. 7c, ΔnleH).
T146	1583-1749	Sentence	denotes	We further examined the role of NleH1 kinase activity using the C. rodentium ΔnleH strain as a background on which to express either wild-type or K159A E. coli NleH1.
T147	1750-1935	Sentence	denotes	Complementing ΔnleH mutant with wild-type NleH1 almost abolished TNF-induced RPS3 S209 phosphorylation whereas complementing with K159A failed to inhibit RPS3 phosphorylation (Fig. 7c).
T148	1936-2050	Sentence	denotes	Collectively, these results demonstrate that NleH1 kinase activity is required to block RPS3 S209 phosphorylation.
T149	2051-2249	Sentence	denotes	We next examined whether the inhibitory activity of NleH1 is sufficiently robust to impair the strong nuclear translocation of RPS3 trigged by the constitutively-active IKKβ (IKKβ [SSEE]) (Fig. 2d).
T150	2250-2420	Sentence	denotes	We found that ectopically expressing either wild-type or SSEE IKKβ proteins, triggered more RPS3 nuclear translocation than the kinase-dead IKKβ (SSAA) protein (Fig. 7d).
T151	2421-2534	Sentence	denotes	RPS3 nuclear accumulation was substantially retarded by infecting cells with wild-type E. coli O157:H7 (Fig. 7d).
T152	2535-2702	Sentence	denotes	In contrast, infecting with either ΔnleH1 or ΔescN strains only slightly impaired RPS3 nuclear translocation in either IKKβ- or IKKβ (SSEE)-expressing cells (Fig. 7d).
T153	2703-2854	Sentence	denotes	As expected, E. coli infections did not affect the RPS3 nuclear translocation in IKKβ (SSAA)-expressing cells, where NF-κB signaling was low (Fig. 7d).
T154	2855-3000	Sentence	denotes	Thus, during infection NleH1 is sufficiently potent to inhibit RPS3 nuclear translocation even in cells expressing constitutively-activated IKKβ.
T155	3001-3117	Sentence	denotes	We examined whether NleH1 could directly phosphorylate IKKβ thus inhibiting IKKβ-mediated RPS3 S209 phosphorylation.
T156	3118-3327	Sentence	denotes	We performed in vitro kinase assays using immunoprecipitated Flag-IKKβ (K44A) as substrate and recombinant His-NleH1 as kinase, so that IKKβ autophosphorylation would not obscure NleH1-induced phosphorylation.
T157	3328-3455	Sentence	denotes	However, we did not observe any detectable 32P incorporation in IKKβ (Supplementary Fig. 13), thus ruling out this possibility.
T158	3456-3542	Sentence	denotes	We then tested the hypothesis that NleH1 could alter the IKKβ substrate specificities.
T159	3543-3635	Sentence	denotes	To this end, we performed in vitro kinase assays using both CK2 and IKK substrates for IKKβ.
T160	3636-3804	Sentence	denotes	As expected, IKKβ phosphorylated RPS3 (Fig. 7e, lane 7) and GST-IκBα (1-54) protein (Fig. 7e, lane 9), demonstrating it harbors either CK2 or IKK substrate specificity.
T161	3805-4005	Sentence	denotes	Preincubation of IKKβ with NleH1 reduced IKKβ-mediated RPS3 phosphorylation, i.e. the CK2 kinase specificity, but not IKKβ-mediated GST-IκBα phosphorylation, i.e. the IKK kinase specificity (Fig. 7e).
T162	4006-4122	Sentence	denotes	Control experiments revealed no NleH1-mediated phosphorylation or autophosphorylation of RPS3 or GST-IκBα (Fig. 7e).
T163	4123-4345	Sentence	denotes	Taken together, NleH1 blocks the CK2 substrate specificity of IKKβ thus inhibiting the IKKβ-mediated RPS3 S209 phosphorylation thus representing a novel strategy by E. coli O157:H7 to alter the host innate immune response.

events-check-again

Id	Subject	Object	Predicate	Lexical cue	Negation	Speculation
T17472	0-5	Protein	denotes	NleH1
T17473	17-21	Protein	denotes	IKKβ
T17474	46-51	Protein	denotes	NleH1
T17475	58-76	Phosphorylation	denotes	autophosphorylated
T17476	108-115	Regulation	denotes	depends
T17477	123-133	Entity	denotes	lysine 159
T17478	177-182	Protein	denotes	NleH1
T17479	183-191	Negative_regulation	denotes	inhibits
T17480	192-196	Protein	denotes	RPS3
T17481	197-201	Entity	denotes	S209
T17482	202-217	Phosphorylation	denotes	phosphorylation
T17483	287-296	Protein	denotes	His-NleH1
T17484	318-327	Protein	denotes	His-NleH1
T17485	361-366	Protein	denotes	NleH1
T17486	370-388	Phosphorylation	denotes	autophosphorylated
T17487	397-402	Entity	denotes	K159A
T17488	409-414	Protein	denotes	NleH1
T17489	415-426	Negative_regulation	denotes	kinase-dead
T17490	487-495	Regulation	denotes	required		true
T17491	500-505	Protein	denotes	NleH1
T17492	509-516	Negative_regulation	denotes	inhibit
T17493	517-521	Protein	denotes	IKKβ
T17494	522-537	Phosphorylation	denotes	phosphorlyation
T17495	541-545	Protein	denotes	RPS3
T17496	549-553	Entity	denotes	S209
T17497	607-612	Protein	denotes	NleH1
T17498	638-643	Protein	denotes	NleH1
T17499	644-654	Gene_expression	denotes	expression
T17500	669-676	Negative_regulation	denotes	reduced
T17501	681-688	Positive_regulation	denotes	induced
T17502	689-693	Protein	denotes	RPS3
T17503	694-698	Entity	denotes	S209
T17504	699-714	Phosphorylation	denotes	phosphorylation
T17505	741-747	Negative_regulation	denotes	failed
T17506	773-778	Protein	denotes	NleH1
T17507	798-806	Positive_regulation	denotes	required
T17508	810-817	Negative_regulation	denotes	protect
T17509	818-822	Protein	denotes	RPS3
T17510	828-832	Protein	denotes	IKKβ
T17511	833-841	Positive_regulation	denotes	mediated
T17512	842-857	Phosphorylation	denotes	phosphorylation
T17513	1001-1005	Protein	denotes	NleH
T17514	1006-1015	Negative_regulation	denotes	inhibited
T17515	1006-1015	Negative_regulation	denotes	inhibited
T17516	1016-1020	Protein	denotes	RPS3
T17517	1021-1028	Entity	denotes	nuclear
T17518	1029-1042	Localization	denotes	translocation
T17519	1047-1051	Protein	denotes	RPS3
T17520	1052-1061	Regulation	denotes	dependent
T17521	1068-1078	Protein	denotes	luciferase
T17522	1079-1087	Positive_regulation	denotes	activity
T17523	1123-1128	Protein	denotes	NleH1
T17524	1150-1154	Protein	denotes	RPS3
T17525	1155-1159	Entity	denotes	S209
T17526	1160-1175	Phosphorylation	denotes	phosphorylation
T17527	1294-1302	Positive_regulation	denotes	increase
T17528	1306-1310	Protein	denotes	RPS3
T17529	1311-1315	Entity	denotes	S209
T17530	1316-1331	Phosphorylation	denotes	phosphorylation
T17531	1348-1360	Positive_regulation	denotes	augmentation
T17532	1364-1368	Protein	denotes	RPS3
T17533	1369-1384	Phosphorylation	denotes	phosphorylation
T17534	1389-1396	Negative_regulation	denotes	reduced
T17535	1466-1470	Protein	denotes	RPS3
T17536	1471-1486	Phosphorylation	denotes	phosphorylation
T17537	1491-1499	Positive_regulation	denotes	enhanced
T17538	1552-1559	Negative_regulation	denotes	lacking
T17539	1560-1564	Protein	denotes	NleH
T17540	1575-1580	Protein	denotes	ΔnleH
T17541	1615-1620	Protein	denotes	NleH1
T17542	1660-1665	Protein	denotes	ΔnleH
T17543	1701-1708	Gene_expression	denotes	express
T17544	1743-1748	Protein	denotes	NleH1
T17545	1764-1769	Protein	denotes	ΔnleH
T17546	1792-1797	Protein	denotes	NleH1
T17547	1805-1814	Negative_regulation	denotes	abolished
T17548	1815-1818	Protein	denotes	TNF
T17549	1819-1826	Positive_regulation	denotes	induced
T17550	1827-1831	Protein	denotes	RPS3
T17551	1832-1836	Entity	denotes	S209
T17552	1837-1852	Phosphorylation	denotes	phosphorylation
T17553	1896-1903	Negative_regulation	denotes	inhibit	true
T17554	1904-1908	Protein	denotes	RPS3
T17555	1909-1924	Phosphorylation	denotes	phosphorylation
T17556	1981-1986	Protein	denotes	NleH1
T17557	2006-2014	Positive_regulation	denotes	required
T17558	2018-2023	Negative_regulation	denotes	block
T17559	2024-2028	Protein	denotes	RPS3
T17560	2029-2033	Entity	denotes	S209
T17561	2034-2049	Phosphorylation	denotes	phosphorylation
T17562	2103-2108	Protein	denotes	NleH1
T17563	2135-2141	Negative_regulation	denotes	impair		true
T17564	2153-2160	Entity	denotes	nuclear
T17565	2161-2174	Localization	denotes	translocation
T17566	2178-2182	Protein	denotes	RPS3
T17567	2183-2190	Positive_regulation	denotes	trigged
T17568	2198-2219	Positive_regulation	denotes	constitutively-active
T17569	2220-2224	Protein	denotes	IKKβ
T17570	2226-2230	Protein	denotes	IKKβ
T17571	2232-2236	Positive_regulation	denotes	SSEE
T17572	2307-2316	Protein	denotes	SSEE IKKβ
T17573	2327-2336	Positive_regulation	denotes	triggered
T17574	2342-2346	Protein	denotes	RPS3
T17575	2347-2354	Entity	denotes	nuclear
T17576	2355-2368	Localization	denotes	translocation
T17577	2378-2389	Negative_regulation	denotes	kinase-dead
T17578	2390-2394	Protein	denotes	IKKβ
T17579	2396-2400	Negative_regulation	denotes	SSAA
T17580	2421-2425	Protein	denotes	RPS3
T17581	2426-2433	Entity	denotes	nuclear
T17582	2434-2446	Localization	denotes	accumulation
T17583	2465-2473	Negative_regulation	denotes	retarded
T17584	2570-2576	Protein	denotes	ΔnleH1
T17585	2580-2585	Protein	denotes	ΔescN
T17586	2617-2621	Protein	denotes	RPS3
T17587	2622-2629	Entity	denotes	nuclear
T17588	2630-2643	Localization	denotes	translocation
T17589	2654-2658	Protein	denotes	IKKβ
T17590	2663-2667	Protein	denotes	IKKβ
T17591	2669-2673	Positive_regulation	denotes	SSEE
T17592	2675-2685	Gene_expression	denotes	expressing
T17593	2675-2685	Gene_expression	denotes	expressing
T17594	2743-2749	Regulation	denotes	affect	true
T17595	2754-2758	Protein	denotes	RPS3
T17596	2759-2766	Entity	denotes	nuclear
T17597	2767-2780	Localization	denotes	translocation
T17598	2784-2788	Protein	denotes	IKKβ
T17599	2790-2794	Negative_regulation	denotes	SSAA
T17600	2796-2806	Gene_expression	denotes	expressing
T17601	2878-2883	Protein	denotes	NleH1
T17602	2910-2917	Negative_regulation	denotes	inhibit
T17603	2918-2922	Protein	denotes	RPS3
T17604	2923-2930	Entity	denotes	nuclear
T17605	2931-2944	Localization	denotes	translocation
T17606	2959-2969	Gene_expression	denotes	expressing
T17607	2985-2994	Positive_regulation	denotes	activated
T17608	2995-2999	Protein	denotes	IKKβ
T17609	3021-3026	Protein	denotes	NleH1
T17610	3042-3055	Phosphorylation	denotes	phosphorylate
T17611	3056-3060	Protein	denotes	IKKβ
T17612	3061-3065	Positive_regulation	denotes	thus		true
T17613	3066-3076	Negative_regulation	denotes	inhibiting
T17614	3077-3081	Protein	denotes	IKKβ
T17615	3082-3090	Positive_regulation	denotes	mediated
T17616	3091-3095	Protein	denotes	RPS3
T17617	3096-3100	Entity	denotes	S209
T17618	3101-3116	Phosphorylation	denotes	phosphorylation
T17619	3179-3195	Protein	denotes	Flag-IKKβ (K44A)
T17620	3225-3234	Protein	denotes	His-NleH1
T17621	3254-3258	Protein	denotes	IKKβ
T17622	3259-3278	Phosphorylation	denotes	autophosphorylation
T17623	3297-3302	Protein	denotes	NleH1
T17624	3311-3326	Phosphorylation	denotes	phosphorylation
T17625	3371-3388	Phosphorylation	denotes	32P incorporation	true
T17626	3392-3396	Protein	denotes	IKKβ
T17627	3491-3496	Protein	denotes	NleH1
T17628	3513-3517	Protein	denotes	IKKβ
T17629	3630-3634	Protein	denotes	IKKβ
T17630	3649-3653	Protein	denotes	IKKβ
T17631	3654-3668	Phosphorylation	denotes	phosphorylated
T17632	3654-3668	Phosphorylation	denotes	phosphorylated
T17633	3669-3673	Protein	denotes	RPS3
T17634	3696-3704	Protein	denotes	GST-IκBα
T17635	3782-3803	Regulation	denotes	substrate specificity
T17636	3822-3826	Protein	denotes	IKKβ
T17637	3832-3837	Protein	denotes	NleH1
T17638	3838-3845	Negative_regulation	denotes	reduced	true
T17639	3838-3845	Negative_regulation	denotes	reduced	true
T17640	3846-3850	Protein	denotes	IKKβ
T17641	3851-3859	Positive_regulation	denotes	mediated
T17642	3860-3864	Protein	denotes	RPS3
T17643	3865-3880	Phosphorylation	denotes	phosphorylation
T17644	3923-3927	Protein	denotes	IKKβ
T17645	3928-3936	Positive_regulation	denotes	mediated
T17646	3937-3945	Protein	denotes	GST-IκBα
T17647	3946-3961	Phosphorylation	denotes	phosphorylation
T17648	4038-4043	Protein	denotes	NleH1
T17649	4044-4052	Positive_regulation	denotes	mediated	true
T17650	4044-4052	Positive_regulation	denotes	mediated	true
T17651	4044-4052	Positive_regulation	denotes	mediated	true
T17652	4044-4052	Positive_regulation	denotes	mediated	true
T17653	4053-4068	Phosphorylation	denotes	phosphorylation
T17654	4053-4068	Phosphorylation	denotes	phosphorylation
T17655	4072-4091	Phosphorylation	denotes	autophosphorylation
T17656	4072-4091	Phosphorylation	denotes	autophosphorylation
T17657	4095-4099	Protein	denotes	RPS3
T17658	4103-4111	Protein	denotes	GST-IκBα
T17659	4139-4144	Protein	denotes	NleH1
T17660	4185-4189	Protein	denotes	IKKβ
T17661	4195-4205	Negative_regulation	denotes	inhibiting
T17662	4210-4214	Protein	denotes	IKKβ
T17663	4215-4223	Positive_regulation	denotes	mediated
T17664	4224-4228	Protein	denotes	RPS3
T17665	4229-4233	Entity	denotes	S209
T17666	4234-4249	Phosphorylation	denotes	phosphorylation
R13949	T17474	T17475	themeOf	NleH1,autophosphorylated
R13951	T17475	T17476	themeOf	autophosphorylated,depends
R13953	T17477	T17476	causeOf	lysine 159,depends
R13954	T17477	T17474	partOf	lysine 159,NleH1
R13957	T17478	T17479	causeOf	NleH1,inhibits
R13959	T17498	T17499	themeOf	NleH1,expression
R13960	T17481	T17482	themeOf	S209,phosphorylation
R13961	T17481	T17480	partOf	S209,RPS3
R13962	T17482	T17479	themeOf	phosphorylation,inhibits
R13963	T17499	T17500	causeOf	expression,reduced
R13964	T17485	T17486	themeOf	NleH1,autophosphorylated
R13965	T17500	T17505	themeOf	reduced,failed
R13966	T17487	T17489	causeOf	K159A,kinase-dead
R13967	T17487	T17488	partOf	K159A,NleH1
R13968	T17488	T17489	themeOf	NleH1,kinase-dead
R13969	T17501	T17500	themeOf	induced,reduced
R13970	T17492	T17490	themeOf	inhibit,required
R13971	T17503	T17504	themeOf	S209,phosphorylation
R13972	T17494	T17492	themeOf	phosphorlyation,inhibit
R13973	T17503	T17502	partOf	S209,RPS3
R13974	T17496	T17494	themeOf	S209,phosphorlyation
R13975	T17496	T17495	partOf	S209,RPS3
R13976	T17504	T17501	themeOf	phosphorylation,induced
R13977	T17508	T17507	themeOf	protect,required
R13978	T17509	T17512	themeOf	RPS3,phosphorylation
R13979	T17572	T17573	causeOf	SSEE IKKβ,triggered
R13980	T17510	T17511	causeOf	IKKβ,mediated
R13981	T17574	T17576	themeOf	RPS3,translocation
R13982	T17511	T17508	themeOf	mediated,protect
R13983	T17575	T17576	locationOf	nuclear,translocation
R13984	T17576	T17573	themeOf	translocation,triggered
R13985	T17512	T17511	themeOf	phosphorylation,mediated
R13986	T17578	T17577	themeOf	IKKβ,kinase-dead
R13987	T17578	T17579	themeOf	IKKβ,SSAA
R13988	T17580	T17582	themeOf	RPS3,accumulation
R13989	T17513	T17514	causeOf	NleH,inhibited
R13990	T17581	T17582	locationOf	nuclear,accumulation
R13991	T17582	T17583	themeOf	accumulation,retarded
R13992	T17513	T17515	causeOf	NleH,inhibited
R13993	T17516	T17518	themeOf	RPS3,translocation
R13994	T17586	T17588	themeOf	RPS3,translocation
R13995	T17587	T17588	locationOf	nuclear,translocation
R13996	T17589	T17593	themeOf	IKKβ,expressing
R13997	T17517	T17518	locationOf	nuclear,translocation
R13998	T17590	T17591	themeOf	IKKβ,SSEE
R13999	T17590	T17592	themeOf	IKKβ,expressing
R14000	T17518	T17514	themeOf	translocation,inhibited
R14001	T17519	T17520	causeOf	RPS3,dependent
R14002	T17520	T17515	themeOf	dependent,inhibited
R14003	T17521	T17522	themeOf	luciferase,activity
R14004	T17595	T17597	themeOf	RPS3,translocation
R14005	T17522	T17520	themeOf	activity,dependent
R14006	T17596	T17597	locationOf	nuclear,translocation
R14007	T17597	T17594	themeOf	translocation,affect
R14008	T17598	T17599	themeOf	IKKβ,SSAA
R14009	T17525	T17526	themeOf	S209,phosphorylation
R14010	T17598	T17600	themeOf	IKKβ,expressing
R14011	T17525	T17524	partOf	S209,RPS3
R14012	T17529	T17530	themeOf	S209,phosphorylation
R14013	T17601	T17602	causeOf	NleH1,inhibit
R14014	T17603	T17605	themeOf	RPS3,translocation
R14015	T17604	T17605	locationOf	nuclear,translocation
R14016	T17605	T17602	themeOf	translocation,inhibit
R14017	T17529	T17528	partOf	S209,RPS3
R14018	T17530	T17527	themeOf	phosphorylation,increase
R14019	T17608	T17606	themeOf	IKKβ,expressing
R14020	T17608	T17607	themeOf	IKKβ,activated
R14021	T17531	T17534	themeOf	augmentation,reduced
R14022	T17532	T17533	themeOf	RPS3,phosphorylation
R14023	T17609	T17613	causeOf	NleH1,inhibiting
R14024	T17610	T17612	causeOf	phosphorylate,thus
R14025	T17533	T17531	themeOf	phosphorylation,augmentation
R14026	T17611	T17610	themeOf	IKKβ,phosphorylate
R14027	T17613	T17612	themeOf	inhibiting,thus
R14028	T17535	T17536	themeOf	RPS3,phosphorylation
R14029	T17614	T17615	causeOf	IKKβ,mediated
R14030	T17615	T17613	themeOf	mediated,inhibiting
R14031	T17536	T17537	themeOf	phosphorylation,enhanced
R14032	T17617	T17618	themeOf	S209,phosphorylation
R14033	T17617	T17616	partOf	S209,RPS3
R14034	T17618	T17615	themeOf	phosphorylation,mediated
R14035	T17538	T17537	causeOf	lacking,enhanced
R14036	T17621	T17622	themeOf	IKKβ,autophosphorylation
R14037	T17621	T17624	themeOf	IKKβ,phosphorylation
R14038	T17539	T17538	themeOf	NleH,lacking
R14039	T17626	T17625	themeOf	IKKβ,32P incorporation
R14040	T17544	T17543	themeOf	NleH1,express
R14041	T17630	T17631	causeOf	IKKβ,phosphorylated
R14042	T17630	T17632	causeOf	IKKβ,phosphorylated
R14043	T17633	T17631	themeOf	RPS3,phosphorylated
R14044	T17633	T17635	themeOf	RPS3,substrate specificity
R14045	T17546	T17547	causeOf	NleH1,abolished
R14046	T17634	T17632	themeOf	GST-IκBα,phosphorylated
R14047	T17637	T17638	causeOf	NleH1,reduced
R14048	T17637	T17639	causeOf	NleH1,reduced
R14049	T17548	T17549	causeOf	TNF,induced
R14050	T17640	T17641	causeOf	IKKβ,mediated
R14051	T17641	T17638	themeOf	mediated,reduced
R14052	T17642	T17643	themeOf	RPS3,phosphorylation
R14053	T17643	T17641	themeOf	phosphorylation,mediated
R14054	T17549	T17547	themeOf	induced,abolished
R14055	T17644	T17645	causeOf	IKKβ,mediated
R14056	T17645	T17639	themeOf	mediated,reduced
R14057	T17551	T17552	themeOf	S209,phosphorylation
R14058	T17646	T17647	themeOf	GST-IκBα,phosphorylation
R14059	T17647	T17645	themeOf	phosphorylation,mediated
R14060	T17648	T17651	causeOf	NleH1,mediated
R14061	T17648	T17652	causeOf	NleH1,mediated
R14062	T17648	T17650	causeOf	NleH1,mediated
R14063	T17648	T17649	causeOf	NleH1,mediated
R14064	T17551	T17550	partOf	S209,RPS3
R14065	T17552	T17549	themeOf	phosphorylation,induced
R14066	T17653	T17652	themeOf	phosphorylation,mediated
R14067	T17654	T17651	themeOf	phosphorylation,mediated
R14068	T17554	T17555	themeOf	RPS3,phosphorylation
R14069	T17655	T17649	themeOf	autophosphorylation,mediated
R14070	T17656	T17650	themeOf	autophosphorylation,mediated
R14071	T17657	T17654	themeOf	RPS3,phosphorylation
R14072	T17657	T17656	themeOf	RPS3,autophosphorylation
R14073	T17658	T17653	themeOf	GST-IκBα,phosphorylation
R14074	T17658	T17655	themeOf	GST-IκBα,autophosphorylation
R14075	T17662	T17663	causeOf	IKKβ,mediated
R14076	T17555	T17553	themeOf	phosphorylation,inhibit
R14077	T17663	T17661	themeOf	mediated,inhibiting
R14078	T17665	T17666	themeOf	S209,phosphorylation
R14079	T17665	T17664	partOf	S209,RPS3
R14080	T17666	T17663	themeOf	phosphorylation,mediated
R14081	T17558	T17557	themeOf	block,required
R14082	T17558	T17563	causeOf	block,impair
R14083	T17560	T17561	themeOf	S209,phosphorylation
R14084	T17560	T17559	partOf	S209,RPS3
R14085	T17561	T17558	themeOf	phosphorylation,block
R14086	T17564	T17565	locationOf	nuclear,translocation
R14089	T17565	T17567	themeOf	translocation,trigged
R14090	T17566	T17565	themeOf	RPS3,translocation
R14093	T17567	T17563	themeOf	trigged,impair
R14095	T17569	T17567	causeOf	IKKβ,trigged
R14099	T17569	T17568	themeOf	IKKβ,constitutively-active
R14100	T17570	T17571	themeOf	IKKβ,SSEE

bionlp-st-ge-2016-reference-tees

Id	Subject	Object	Predicate	Lexical cue
T16750	0-5	Protein	denotes	NleH1
T16751	17-21	Protein	denotes	IKKβ
T16752	46-51	Protein	denotes	NleH1
T16753	177-182	Protein	denotes	NleH1
T16754	192-201	Protein	denotes	RPS3 S209
T16755	291-296	Protein	denotes	NleH1
T16756	361-366	Protein	denotes	NleH1
T16757	409-433	Protein	denotes	NleH1 kinase-dead mutant
T16758	435-442	Protein	denotes	Fig. 7a
T16759	202-217	Phosphorylation	denotes	phosphorylation
T16760	370-388	Phosphorylation	denotes	autophosphorylated
T16761	370-388	Phosphorylation	denotes	autophosphorylated
T16762	370-388	Phosphorylation	denotes	autophosphorylated
T16763	183-191	Negative_regulation	denotes	inhibits
T16764	500-505	Protein	denotes	NleH1
T16765	517-521	Protein	denotes	IKKβ
T16766	541-545	Protein	denotes	RPS3
T16767	601-612	Protein	denotes	K159A NleH1
T16768	522-537	Phosphorylation	denotes	phosphorlyation
T16769	522-537	Phosphorylation	denotes	phosphorlyation
T16770	570-580	Gene_expression	denotes	expressing
T16771	509-516	Negative_regulation	denotes	inhibit
T16772	509-516	Negative_regulation	denotes	inhibit
T16773	638-643	Protein	denotes	NleH1
T16774	677-680	Protein	denotes	TNF
T16775	689-698	Protein	denotes	RPS3 S209
T16776	758-765	Protein	denotes	Fig. 7b
T16777	644-654	Gene_expression	denotes	expression
T16778	699-714	Phosphorylation	denotes	phosphorylation
T16779	681-688	Positive_regulation	denotes	induced
T16780	669-676	Negative_regulation	denotes	reduced
T16781	773-785	Protein	denotes	NleH1 kinase
T16782	818-822	Protein	denotes	RPS3
T16783	828-832	Protein	denotes	IKKβ
T16784	842-857	Phosphorylation	denotes	phosphorylation
T16785	988-1005	Protein	denotes	C. rodentium NleH
T16786	1016-1020	Protein	denotes	RPS3
T16787	1047-1051	Protein	denotes	RPS3
T16788	1065-1078	Protein	denotes	κB luciferase
T16789	1123-1128	Protein	denotes	NleH1
T16790	1021-1028	Entity	denotes	nuclear
T16791	1029-1042	Localization	denotes	translocation
T16792	1029-1042	Localization	denotes	translocation
T16793	1006-1015	Negative_regulation	denotes	inhibited
T16794	1006-1015	Negative_regulation	denotes	inhibited
T16795	1150-1159	Protein	denotes	RPS3 S209
T16796	1160-1175	Phosphorylation	denotes	phosphorylation
T16797	1257-1260	Protein	denotes	TNF
T16798	1306-1315	Protein	denotes	RPS3 S209
T16799	1333-1340	Protein	denotes	Fig. 7c
T16800	1316-1331	Phosphorylation	denotes	phosphorylation
T16801	1294-1302	Positive_regulation	denotes	increase
T16802	1364-1368	Protein	denotes	RPS3
T16803	1369-1384	Phosphorylation	denotes	phosphorylation
T16804	1348-1360	Positive_regulation	denotes	augmentation
T16805	1389-1396	Negative_regulation	denotes	reduced
T16806	1466-1470	Protein	denotes	RPS3
T16807	1560-1581	Protein	denotes	NleH (Fig. 7c, ΔnleH)
T16808	1471-1486	Phosphorylation	denotes	phosphorylation
T16809	1491-1499	Positive_regulation	denotes	enhanced
T16810	1552-1559	Negative_regulation	denotes	lacking
T16811	1615-1627	Protein	denotes	NleH1 kinase
T16812	1729-1748	Protein	denotes	K159A E. coli NleH1
T16813	1701-1708	Gene_expression	denotes	express
T16814	1750-1776	Protein	denotes	Complementing ΔnleH mutant
T16815	1815-1818	Protein	denotes	TNF
T16816	1827-1836	Protein	denotes	RPS3 S209
T16817	1904-1908	Protein	denotes	RPS3
T16818	1926-1933	Protein	denotes	Fig. 7c
T16819	1837-1852	Phosphorylation	denotes	phosphorylation
T16820	1909-1924	Phosphorylation	denotes	phosphorylation
T16821	1909-1924	Phosphorylation	denotes	phosphorylation
T16822	1819-1826	Positive_regulation	denotes	induced
T16823	1896-1903	Negative_regulation	denotes	inhibit
T16824	1896-1903	Negative_regulation	denotes	inhibit
T16825	1805-1814	Negative_regulation	denotes	abolished
T16826	1981-1993	Protein	denotes	NleH1 kinase
T16827	2024-2028	Protein	denotes	RPS3
T16828	2029-2033	Protein	denotes	S209
T16829	2034-2049	Phosphorylation	denotes	phosphorylation
T16830	2034-2049	Phosphorylation	denotes	phosphorylation
T16831	2018-2023	Negative_regulation	denotes	block
T16832	2018-2023	Negative_regulation	denotes	block
T16833	2103-2108	Protein	denotes	NleH1
T16834	2178-2182	Protein	denotes	RPS3
T16835	2220-2224	Protein	denotes	IKKβ
T16836	2226-2230	Protein	denotes	IKKβ
T16837	2153-2160	Entity	denotes	nuclear
T16838	2161-2174	Localization	denotes	translocation
T16839	2135-2141	Negative_regulation	denotes	impair
T16840	2307-2325	Protein	denotes	SSEE IKKβ proteins
T16841	2342-2346	Protein	denotes	RPS3
T16842	2390-2394	Protein	denotes	IKKβ
T16843	2276-2286	Gene_expression	denotes	expressing
T16844	2347-2354	Entity	denotes	nuclear
T16845	2355-2368	Localization	denotes	translocation
T16846	2355-2368	Localization	denotes	translocation
T16847	2421-2425	Protein	denotes	RPS3
T16848	2426-2433	Entity	denotes	nuclear
T16849	2434-2446	Localization	denotes	accumulation
T16850	2617-2621	Protein	denotes	RPS3
T16851	2654-2667	Protein	denotes	IKKβ- or IKKβ
T16852	2669-2673	Protein	denotes	SSEE
T16853	2622-2629	Entity	denotes	nuclear
T16854	2630-2643	Localization	denotes	translocation
T16855	2674-2685	Gene_expression	denotes	-expressing
T16856	2674-2685	Gene_expression	denotes	-expressing
T16857	2608-2616	Negative_regulation	denotes	impaired
T16858	2754-2758	Protein	denotes	RPS3
T16859	2784-2788	Protein	denotes	IKKβ
T16860	2820-2825	Protein	denotes	NF-κB
T16861	2759-2766	Entity	denotes	nuclear
T16862	2767-2780	Localization	denotes	translocation
T16863	2795-2806	Gene_expression	denotes	-expressing
T16864	2743-2749	Regulation	denotes	affect
T16865	2918-2922	Protein	denotes	RPS3
T16866	2995-2999	Protein	denotes	IKKβ
T16867	2923-2930	Entity	denotes	nuclear
T16868	2931-2944	Localization	denotes	translocation
T16869	2959-2969	Gene_expression	denotes	expressing
T16870	2910-2917	Negative_regulation	denotes	inhibit
T16871	3021-3026	Protein	denotes	NleH1
T16872	3056-3060	Protein	denotes	IKKβ
T16873	3077-3081	Protein	denotes	IKKβ
T16874	3091-3095	Protein	denotes	RPS3
T16875	3096-3100	Protein	denotes	S209
T16876	3042-3055	Phosphorylation	denotes	phosphorylate
T16877	3101-3116	Phosphorylation	denotes	phosphorylation
T16878	3101-3116	Phosphorylation	denotes	phosphorylation
T16879	3066-3076	Negative_regulation	denotes	inhibiting
T16880	3066-3076	Negative_regulation	denotes	inhibiting
T16881	3082-3090	Positive_regulation	denotes	mediated
T16882	3082-3090	Positive_regulation	denotes	mediated
T16883	3179-3183	Protein	denotes	Flag
T16884	3184-3188	Protein	denotes	IKKβ
T16885	3229-3234	Protein	denotes	NleH1
T16886	3254-3258	Protein	denotes	IKKβ
T16887	3259-3278	Phosphorylation	denotes	autophosphorylation
T16888	3311-3326	Phosphorylation	denotes	phosphorylation
T16889	3311-3326	Phosphorylation	denotes	phosphorylation
T16890	3392-3396	Protein	denotes	IKKβ
T16891	3491-3496	Protein	denotes	NleH1
T16892	3513-3517	Protein	denotes	IKKβ
T16893	3603-3606	Protein	denotes	CK2
T16894	3611-3614	Protein	denotes	IKK
T16895	3630-3634	Protein	denotes	IKKβ
T16896	3649-3653	Protein	denotes	IKKβ
T16897	3669-3673	Protein	denotes	RPS3
T16898	3675-3678	Protein	denotes	Fig
T16899	3696-3719	Protein	denotes	GST-IκBα (1-54) protein
T16900	3721-3724	Protein	denotes	Fig
T16901	3771-3774	Protein	denotes	CK2
T16902	3778-3781	Protein	denotes	IKK
T16903	3654-3668	Phosphorylation	denotes	phosphorylated
T16904	3654-3668	Phosphorylation	denotes	phosphorylated
T16905	3654-3668	Phosphorylation	denotes	phosphorylated
T16906	3654-3668	Phosphorylation	denotes	phosphorylated
T16907	3822-3826	Protein	denotes	IKKβ
T16908	3832-3837	Protein	denotes	NleH1
T16909	3846-3850	Protein	denotes	IKKβ
T16910	3860-3864	Protein	denotes	RPS3
T16911	3891-3901	Protein	denotes	CK2 kinase
T16920	3851-3859	Positive_regulation	denotes	mediated
T16921	3928-3936	Positive_regulation	denotes	mediated
T16922	3928-3936	Positive_regulation	denotes	mediated
T16923	4038-4043	Protein	denotes	NleH1
T16924	4095-4099	Protein	denotes	RPS3
T16925	4103-4111	Protein	denotes	GST-IκBα
T16926	4113-4120	Protein	denotes	Fig. 7e
T16927	4053-4068	Phosphorylation	denotes	phosphorylation
T16928	4053-4068	Phosphorylation	denotes	phosphorylation
T16929	4053-4068	Phosphorylation	denotes	phosphorylation
T16930	4053-4068	Phosphorylation	denotes	phosphorylation
T16931	4072-4091	Phosphorylation	denotes	autophosphorylation
T17276	4072-4091	Phosphorylation	denotes	autophosphorylation
T17277	4072-4091	Phosphorylation	denotes	autophosphorylation
T17278	4139-4144	Protein	denotes	NleH1
T17279	4156-4159	Protein	denotes	CK2
T17280	4185-4189	Protein	denotes	IKKβ
T17281	4210-4214	Protein	denotes	IKKβ
T17282	4224-4233	Protein	denotes	RPS3 S209
T17283	4234-4249	Phosphorylation	denotes	phosphorylation
T17284	4195-4205	Negative_regulation	denotes	inhibiting
T17285	4215-4223	Positive_regulation	denotes	mediated
T16912	3923-3927	Protein	denotes	IKKβ
T16913	3937-3940	Protein	denotes	GST
T16914	3941-3945	Protein	denotes	IκBα
T16915	3972-3982	Protein	denotes	IKK kinase
T16916	3996-4003	Protein	denotes	Fig. 7e
T16917	3865-3880	Phosphorylation	denotes	phosphorylation
T16918	3946-3961	Phosphorylation	denotes	phosphorylation
T16919	3946-3961	Phosphorylation	denotes	phosphorylation
R13508	T16753	T16763	causeOf	NleH1,inhibits
R13509	T16754	T16759	themeOf	RPS3 S209,phosphorylation
R13510	T16756	T16760	themeOf	NleH1,autophosphorylated
R13511	T16757	T16761	themeOf	NleH1 kinase-dead mutant,autophosphorylated
R13512	T16758	T16762	themeOf	Fig. 7a,autophosphorylated
R13513	T16759	T16763	themeOf	phosphorylation,inhibits
R13514	T16764	T16771	causeOf	NleH1,inhibit
R13515	T16764	T16772	causeOf	NleH1,inhibit
R13516	T16765	T16768	themeOf	IKKβ,phosphorlyation
R13517	T16766	T16769	themeOf	RPS3,phosphorlyation
R13518	T16767	T16770	themeOf	K159A NleH1,expressing
R13519	T16768	T16771	themeOf	phosphorlyation,inhibit
R13520	T16769	T16772	themeOf	phosphorlyation,inhibit
R13521	T16773	T16777	themeOf	NleH1,expression
R13522	T16774	T16779	causeOf	TNF,induced
R13523	T16775	T16778	themeOf	RPS3 S209,phosphorylation
R13524	T16777	T16780	causeOf	expression,reduced
R13525	T16778	T16779	themeOf	phosphorylation,induced
R13526	T16779	T16780	themeOf	induced,reduced
R13527	T16783	T16784	themeOf	IKKβ,phosphorylation
R13528	T16786	T16791	themeOf	RPS3,translocation
R13529	T16788	T16792	themeOf	κB luciferase,translocation
R13530	T16790	T16791	locationOf	nuclear,translocation
R13531	T16790	T16792	locationOf	nuclear,translocation
R13532	T16791	T16793	themeOf	translocation,inhibited
R13533	T16792	T16794	themeOf	translocation,inhibited
R13534	T16795	T16796	themeOf	RPS3 S209,phosphorylation
R13535	T16798	T16800	themeOf	RPS3 S209,phosphorylation
R13536	T16800	T16801	themeOf	phosphorylation,increase
R13537	T16802	T16803	themeOf	RPS3,phosphorylation
R13538	T16803	T16804	themeOf	phosphorylation,augmentation
R13539	T16804	T16805	themeOf	augmentation,reduced
R13540	T16806	T16808	themeOf	RPS3,phosphorylation
R13541	T16807	T16810	themeOf	"NleH (Fig. 7c, ΔnleH)",lacking
R13542	T16808	T16809	themeOf	phosphorylation,enhanced
R13543	T16812	T16813	themeOf	K159A E. coli NleH1,express
R13544	T16814	T16825	causeOf	Complementing ΔnleH mutant,abolished
R13545	T16815	T16822	causeOf	TNF,induced
R13546	T16816	T16819	themeOf	RPS3 S209,phosphorylation
R13547	T16817	T16820	themeOf	RPS3,phosphorylation
R13548	T16818	T16821	themeOf	Fig. 7c,phosphorylation
R13549	T16819	T16822	themeOf	phosphorylation,induced
R13550	T16820	T16823	themeOf	phosphorylation,inhibit
R13551	T16821	T16824	themeOf	phosphorylation,inhibit
R13552	T16822	T16825	themeOf	induced,abolished
R13553	T16827	T16829	themeOf	RPS3,phosphorylation
R13554	T16828	T16830	themeOf	S209,phosphorylation
R13555	T16829	T16831	themeOf	phosphorylation,block
R13556	T16830	T16832	themeOf	phosphorylation,block
R13557	T16834	T16838	themeOf	RPS3,translocation
R13558	T16837	T16838	locationOf	nuclear,translocation
R13559	T16838	T16839	themeOf	translocation,impair
R13560	T16840	T16843	themeOf	SSEE IKKβ proteins,expressing
R13561	T16841	T16845	themeOf	RPS3,translocation
R13562	T16842	T16846	themeOf	IKKβ,translocation
R13563	T16844	T16845	locationOf	nuclear,translocation
R13564	T16844	T16846	locationOf	nuclear,translocation
R13565	T16847	T16849	themeOf	RPS3,accumulation
R13566	T16848	T16849	locationOf	nuclear,accumulation
R13567	T16850	T16854	themeOf	RPS3,translocation
R13568	T16851	T16855	themeOf	IKKβ- or IKKβ,-expressing
R13569	T16852	T16856	themeOf	SSEE,-expressing
R13570	T16853	T16854	locationOf	nuclear,translocation
R13571	T16854	T16857	themeOf	translocation,impaired
R13572	T16858	T16862	themeOf	RPS3,translocation
R13573	T16859	T16863	themeOf	IKKβ,-expressing
R13574	T16861	T16862	locationOf	nuclear,translocation
R13575	T16862	T16864	themeOf	translocation,affect
R13576	T16865	T16868	themeOf	RPS3,translocation
R13577	T16866	T16869	themeOf	IKKβ,expressing
R13578	T16867	T16868	locationOf	nuclear,translocation
R13579	T16868	T16870	themeOf	translocation,inhibit
R13580	T16871	T16876	themeOf	NleH1,phosphorylate
R13581	T16873	T16881	causeOf	IKKβ,mediated
R13582	T16873	T16882	causeOf	IKKβ,mediated
R13583	T16874	T16877	themeOf	RPS3,phosphorylation
R13584	T16875	T16878	themeOf	S209,phosphorylation
R13585	T16877	T16879	themeOf	phosphorylation,inhibiting
R13586	T16877	T16881	themeOf	phosphorylation,mediated
R13587	T16878	T16880	themeOf	phosphorylation,inhibiting
R13588	T16878	T16882	themeOf	phosphorylation,mediated
R13589	T16883	T16888	themeOf	Flag,phosphorylation
R13590	T16886	T16887	themeOf	IKKβ,autophosphorylation
R13591	T16886	T16889	themeOf	IKKβ,phosphorylation
R13592	T16897	T16903	themeOf	RPS3,phosphorylated
R13593	T16898	T16904	themeOf	Fig,phosphorylated
R13594	T16899	T16905	themeOf	GST-IκBα (1-54) protein,phosphorylated
R13595	T16900	T16906	themeOf	Fig,phosphorylated
R13596	T16909	T16920	causeOf	IKKβ,mediated
R13597	T16910	T16917	themeOf	RPS3,phosphorylation
R13598	T16912	T16921	causeOf	IKKβ,mediated
R13599	T16912	T16922	causeOf	IKKβ,mediated
R13600	T16913	T16918	themeOf	GST,phosphorylation
R13601	T16914	T16919	themeOf	IκBα,phosphorylation
R13602	T16917	T16920	themeOf	phosphorylation,mediated
R13603	T16918	T16921	themeOf	phosphorylation,mediated
R13604	T16919	T16922	themeOf	phosphorylation,mediated
R13605	T16923	T16927	themeOf	NleH1,phosphorylation
R13606	T16924	T16928	themeOf	RPS3,phosphorylation
R13607	T16924	T16931	themeOf	RPS3,autophosphorylation
R13608	T16925	T16929	themeOf	GST-IκBα,phosphorylation
R13609	T16925	T17276	themeOf	GST-IκBα,autophosphorylation
R13610	T16926	T16930	themeOf	Fig. 7e,phosphorylation
R13611	T16926	T17277	themeOf	Fig. 7e,autophosphorylation
R13785	T17281	T17285	causeOf	IKKβ,mediated
R13795	T17282	T17283	themeOf	RPS3 S209,phosphorylation
R13799	T17283	T17284	themeOf	phosphorylation,inhibiting
R13802	T17283	T17285	themeOf	phosphorylation,mediated

bionlp-st-ge-2016-reference

Id	Subject	Object	Predicate	Lexical cue	Negation	Speculation
T14712	58-76	Phosphorylation	denotes	autophosphorylated
T14713	108-115	Regulation	denotes	depends
T14714	123-133	Entity	denotes	lysine 159
T14715	177-182	Protein	denotes	NleH1
T14716	183-191	Negative_regulation	denotes	inhibits
T14717	192-196	Protein	denotes	RPS3
T14718	197-201	Entity	denotes	S209
T14719	202-217	Phosphorylation	denotes	phosphorylation
T14720	287-296	Protein	denotes	His-NleH1
T14721	318-327	Protein	denotes	His-NleH1
T14722	361-366	Protein	denotes	NleH1
T14723	370-388	Phosphorylation	denotes	autophosphorylated
T14724	397-402	Entity	denotes	K159A
T14725	409-414	Protein	denotes	NleH1
T14726	415-426	Negative_regulation	denotes	kinase-dead
T14727	487-495	Regulation	denotes	required		true
T14728	500-505	Protein	denotes	NleH1
T14729	509-516	Negative_regulation	denotes	inhibit
T14730	517-521	Protein	denotes	IKKβ
T14731	522-537	Phosphorylation	denotes	phosphorlyation
T14732	541-545	Protein	denotes	RPS3
T14733	549-553	Entity	denotes	S209
T14734	607-612	Protein	denotes	NleH1
T14735	638-643	Protein	denotes	NleH1
T14736	644-654	Gene_expression	denotes	expression
T14737	669-676	Negative_regulation	denotes	reduced
T14738	681-688	Positive_regulation	denotes	induced
T14739	689-693	Protein	denotes	RPS3
T14740	694-698	Entity	denotes	S209
T14741	699-714	Phosphorylation	denotes	phosphorylation
T14742	741-747	Negative_regulation	denotes	failed
T14743	773-778	Protein	denotes	NleH1
T14744	798-806	Positive_regulation	denotes	required
T14745	810-817	Negative_regulation	denotes	protect
T14746	818-822	Protein	denotes	RPS3
T14747	828-832	Protein	denotes	IKKβ
T14748	833-841	Positive_regulation	denotes	mediated
T14749	842-857	Phosphorylation	denotes	phosphorylation
T14750	1001-1005	Protein	denotes	NleH
T14751	1006-1015	Negative_regulation	denotes	inhibited
T14752	1006-1015	Negative_regulation	denotes	inhibited
T14753	1016-1020	Protein	denotes	RPS3
T14754	1021-1028	Entity	denotes	nuclear
T14755	1029-1042	Localization	denotes	translocation
T14756	1047-1051	Protein	denotes	RPS3
T14757	1052-1061	Regulation	denotes	dependent
T14758	1068-1078	Protein	denotes	luciferase
T14759	1079-1087	Positive_regulation	denotes	activity
T14760	1123-1128	Protein	denotes	NleH1
T14761	1150-1154	Protein	denotes	RPS3
T14762	1155-1159	Entity	denotes	S209
T14763	1160-1175	Phosphorylation	denotes	phosphorylation
T14764	1294-1302	Positive_regulation	denotes	increase
T14765	1306-1310	Protein	denotes	RPS3
T14766	1311-1315	Entity	denotes	S209
T14767	1316-1331	Phosphorylation	denotes	phosphorylation
T14768	1348-1360	Positive_regulation	denotes	augmentation
T14769	1364-1368	Protein	denotes	RPS3
T14770	1369-1384	Phosphorylation	denotes	phosphorylation
T14771	1389-1396	Negative_regulation	denotes	reduced
T14772	1466-1470	Protein	denotes	RPS3
T14773	1471-1486	Phosphorylation	denotes	phosphorylation
T14774	1491-1499	Positive_regulation	denotes	enhanced
T14775	1552-1559	Negative_regulation	denotes	lacking
T14776	1560-1564	Protein	denotes	NleH
T14777	1575-1580	Protein	denotes	ΔnleH
T14778	1615-1620	Protein	denotes	NleH1
T14779	1660-1665	Protein	denotes	ΔnleH
T14780	1701-1708	Gene_expression	denotes	express
T14781	1743-1748	Protein	denotes	NleH1
T14782	1764-1769	Protein	denotes	ΔnleH
T14783	1792-1797	Protein	denotes	NleH1
T14784	1805-1814	Negative_regulation	denotes	abolished
T14785	1815-1818	Protein	denotes	TNF
T14786	1819-1826	Positive_regulation	denotes	induced
T14787	1827-1831	Protein	denotes	RPS3
T14788	1832-1836	Entity	denotes	S209
T14789	1837-1852	Phosphorylation	denotes	phosphorylation
T14790	1896-1903	Negative_regulation	denotes	inhibit	true
T14791	1904-1908	Protein	denotes	RPS3
T14792	1909-1924	Phosphorylation	denotes	phosphorylation
T14793	1981-1986	Protein	denotes	NleH1
T14794	2006-2014	Positive_regulation	denotes	required
T14795	2018-2023	Negative_regulation	denotes	block
T14796	2024-2028	Protein	denotes	RPS3
T14797	2029-2033	Entity	denotes	S209
T14798	2034-2049	Phosphorylation	denotes	phosphorylation
T14799	2103-2108	Protein	denotes	NleH1
T14800	2135-2141	Negative_regulation	denotes	impair		true
T14801	2153-2160	Entity	denotes	nuclear
T14802	2161-2174	Localization	denotes	translocation
T14803	2178-2182	Protein	denotes	RPS3
T14804	2183-2190	Positive_regulation	denotes	trigged
T14805	2198-2219	Positive_regulation	denotes	constitutively-active
T14806	2220-2224	Protein	denotes	IKKβ
T14807	2226-2230	Protein	denotes	IKKβ
T14808	2232-2236	Positive_regulation	denotes	SSEE
T14809	2307-2316	Protein	denotes	SSEE IKKβ
T14810	2327-2336	Positive_regulation	denotes	triggered
T14811	2342-2346	Protein	denotes	RPS3
T14812	2347-2354	Entity	denotes	nuclear
T14813	2355-2368	Localization	denotes	translocation
T14814	2378-2389	Negative_regulation	denotes	kinase-dead
T14815	2390-2394	Protein	denotes	IKKβ
T14816	2396-2400	Negative_regulation	denotes	SSAA
T14817	2421-2425	Protein	denotes	RPS3
T14818	2426-2433	Entity	denotes	nuclear
T14819	2434-2446	Localization	denotes	accumulation
T14820	2465-2473	Negative_regulation	denotes	retarded
T14821	2570-2576	Protein	denotes	ΔnleH1
T14822	2580-2585	Protein	denotes	ΔescN
T14823	2617-2621	Protein	denotes	RPS3
T14824	2622-2629	Entity	denotes	nuclear
T14825	2630-2643	Localization	denotes	translocation
T14826	2654-2658	Protein	denotes	IKKβ
T14827	2663-2667	Protein	denotes	IKKβ
T14828	2669-2673	Positive_regulation	denotes	SSEE
T14829	2675-2685	Gene_expression	denotes	expressing
T14830	2675-2685	Gene_expression	denotes	expressing
T14831	2743-2749	Regulation	denotes	affect	true
T14832	2754-2758	Protein	denotes	RPS3
T14833	2759-2766	Entity	denotes	nuclear
T14834	2767-2780	Localization	denotes	translocation
T14835	2784-2788	Protein	denotes	IKKβ
T14836	2790-2794	Negative_regulation	denotes	SSAA
T14837	2796-2806	Gene_expression	denotes	expressing
T14838	2878-2883	Protein	denotes	NleH1
T14839	2910-2917	Negative_regulation	denotes	inhibit
T14840	2918-2922	Protein	denotes	RPS3
T14841	2923-2930	Entity	denotes	nuclear
T14842	2931-2944	Localization	denotes	translocation
T14843	2959-2969	Gene_expression	denotes	expressing
T14844	2985-2994	Positive_regulation	denotes	activated
T14845	2995-2999	Protein	denotes	IKKβ
T14846	3021-3026	Protein	denotes	NleH1
T14847	3042-3055	Phosphorylation	denotes	phosphorylate
T14848	3056-3060	Protein	denotes	IKKβ
T14849	3061-3065	Positive_regulation	denotes	thus		true
T14850	3066-3076	Negative_regulation	denotes	inhibiting
T14851	3077-3081	Protein	denotes	IKKβ
T14852	3082-3090	Positive_regulation	denotes	mediated
T14853	3091-3095	Protein	denotes	RPS3
T14854	3096-3100	Entity	denotes	S209
T14855	3101-3116	Phosphorylation	denotes	phosphorylation
T14856	3179-3195	Protein	denotes	Flag-IKKβ (K44A)
T14857	3225-3234	Protein	denotes	His-NleH1
T14858	3254-3258	Protein	denotes	IKKβ
T14859	3259-3278	Phosphorylation	denotes	autophosphorylation
T14860	3297-3302	Protein	denotes	NleH1
T14861	3311-3326	Phosphorylation	denotes	phosphorylation
T14862	3371-3388	Phosphorylation	denotes	32P incorporation	true
T14863	3392-3396	Protein	denotes	IKKβ
T14864	3491-3496	Protein	denotes	NleH1
T14865	3513-3517	Protein	denotes	IKKβ
T14866	3630-3634	Protein	denotes	IKKβ
T14867	3649-3653	Protein	denotes	IKKβ
T14868	3654-3668	Phosphorylation	denotes	phosphorylated
T14869	3654-3668	Phosphorylation	denotes	phosphorylated
T14870	3669-3673	Protein	denotes	RPS3
T14871	3696-3704	Protein	denotes	GST-IκBα
T14872	3782-3803	Regulation	denotes	substrate specificity
T14873	3822-3826	Protein	denotes	IKKβ
T14874	3832-3837	Protein	denotes	NleH1
T14875	3838-3845	Negative_regulation	denotes	reduced	true
T14876	3838-3845	Negative_regulation	denotes	reduced	true
T14877	3846-3850	Protein	denotes	IKKβ
T14878	3851-3859	Positive_regulation	denotes	mediated
T14879	3860-3864	Protein	denotes	RPS3
T14880	3865-3880	Phosphorylation	denotes	phosphorylation
T14881	3923-3927	Protein	denotes	IKKβ
T14882	3928-3936	Positive_regulation	denotes	mediated
T14883	3937-3945	Protein	denotes	GST-IκBα
T14884	3946-3961	Phosphorylation	denotes	phosphorylation
T14885	4038-4043	Protein	denotes	NleH1
T14886	4044-4052	Positive_regulation	denotes	mediated	true
T14887	4044-4052	Positive_regulation	denotes	mediated	true
T14888	4044-4052	Positive_regulation	denotes	mediated	true
T14889	4044-4052	Positive_regulation	denotes	mediated	true
T14890	4053-4068	Phosphorylation	denotes	phosphorylation
T14891	4053-4068	Phosphorylation	denotes	phosphorylation
T14892	4072-4091	Phosphorylation	denotes	autophosphorylation
T14893	4072-4091	Phosphorylation	denotes	autophosphorylation
T14894	4095-4099	Protein	denotes	RPS3
T14895	4103-4111	Protein	denotes	GST-IκBα
T14896	4139-4144	Protein	denotes	NleH1
T14897	4185-4189	Protein	denotes	IKKβ
T14898	4195-4205	Negative_regulation	denotes	inhibiting
T14899	4210-4214	Protein	denotes	IKKβ
T14900	4215-4223	Positive_regulation	denotes	mediated
T14901	4224-4228	Protein	denotes	RPS3
T14902	4229-4233	Entity	denotes	S209
T14903	4234-4249	Phosphorylation	denotes	phosphorylation
T14709	0-5	Protein	denotes	NleH1
T14710	17-21	Protein	denotes	IKKβ
T14711	46-51	Protein	denotes	NleH1
R11787	T14711	T14712	themeOf	NleH1,autophosphorylated
R11788	T14712	T14713	themeOf	autophosphorylated,depends
R11789	T14714	T14713	causeOf	lysine 159,depends
R11790	T14714	T14711	partOf	lysine 159,NleH1
R11793	T14718	T14717	partOf	S209,RPS3
R11794	T14719	T14716	themeOf	phosphorylation,inhibits
R11795	T14722	T14723	themeOf	NleH1,autophosphorylated
R11796	T14724	T14726	causeOf	K159A,kinase-dead
R11797	T14724	T14725	partOf	K159A,NleH1
R11798	T14725	T14726	themeOf	NleH1,kinase-dead
R11799	T14729	T14727	themeOf	inhibit,required
R11800	T14731	T14729	themeOf	phosphorlyation,inhibit
R11801	T14733	T14731	themeOf	S209,phosphorlyation
R11802	T14733	T14732	partOf	S209,RPS3
R11803	T14735	T14736	themeOf	NleH1,expression
R11804	T14736	T14737	causeOf	expression,reduced
R11805	T14737	T14742	themeOf	reduced,failed
R11806	T14738	T14737	themeOf	induced,reduced
R11807	T14740	T14741	themeOf	S209,phosphorylation
R11808	T14740	T14739	partOf	S209,RPS3
R11809	T14741	T14738	themeOf	phosphorylation,induced
R11810	T14745	T14744	themeOf	protect,required
R11811	T14746	T14749	themeOf	RPS3,phosphorylation
R11812	T14747	T14748	causeOf	IKKβ,mediated
R11813	T14748	T14745	themeOf	mediated,protect
R11814	T14749	T14748	themeOf	phosphorylation,mediated
R11815	T14750	T14751	causeOf	NleH,inhibited
R11816	T14750	T14752	causeOf	NleH,inhibited
R11817	T14753	T14755	themeOf	RPS3,translocation
R11818	T14754	T14755	locationOf	nuclear,translocation
R11819	T14755	T14751	themeOf	translocation,inhibited
R11820	T14756	T14757	causeOf	RPS3,dependent
R11821	T14757	T14752	themeOf	dependent,inhibited
R11822	T14758	T14759	themeOf	luciferase,activity
R11823	T14759	T14757	themeOf	activity,dependent
R11824	T14762	T14763	themeOf	S209,phosphorylation
R11825	T14762	T14761	partOf	S209,RPS3
R11826	T14766	T14767	themeOf	S209,phosphorylation
R11827	T14766	T14765	partOf	S209,RPS3
R11828	T14767	T14764	themeOf	phosphorylation,increase
R11829	T14768	T14771	themeOf	augmentation,reduced
R11830	T14769	T14770	themeOf	RPS3,phosphorylation
R11831	T14770	T14768	themeOf	phosphorylation,augmentation
R11832	T14772	T14773	themeOf	RPS3,phosphorylation
R11833	T14773	T14774	themeOf	phosphorylation,enhanced
R11834	T14775	T14774	causeOf	lacking,enhanced
R11835	T14776	T14775	themeOf	NleH,lacking
R11836	T14781	T14780	themeOf	NleH1,express
R11837	T14783	T14784	causeOf	NleH1,abolished
R11838	T14785	T14786	causeOf	TNF,induced
R11839	T14786	T14784	themeOf	induced,abolished
R11840	T14788	T14789	themeOf	S209,phosphorylation
R11841	T14788	T14787	partOf	S209,RPS3
R11842	T14789	T14786	themeOf	phosphorylation,induced
R11843	T14791	T14792	themeOf	RPS3,phosphorylation
R11844	T14792	T14790	themeOf	phosphorylation,inhibit
R11845	T14795	T14794	themeOf	block,required
R11846	T14795	T14800	causeOf	block,impair
R11847	T14797	T14798	themeOf	S209,phosphorylation
R11848	T14797	T14796	partOf	S209,RPS3
R11849	T14798	T14795	themeOf	phosphorylation,block
R11850	T14801	T14802	locationOf	nuclear,translocation
R11851	T14802	T14804	themeOf	translocation,trigged
R11852	T14803	T14802	themeOf	RPS3,translocation
R11853	T14804	T14800	themeOf	trigged,impair
R11854	T14806	T14804	causeOf	IKKβ,trigged
R11855	T14806	T14805	themeOf	IKKβ,constitutively-active
R11856	T14807	T14808	themeOf	IKKβ,SSEE
R11857	T14809	T14810	causeOf	SSEE IKKβ,triggered
R11858	T14811	T14813	themeOf	RPS3,translocation
R11859	T14812	T14813	locationOf	nuclear,translocation
R11860	T14813	T14810	themeOf	translocation,triggered
R11861	T14815	T14814	themeOf	IKKβ,kinase-dead
R11862	T14815	T14816	themeOf	IKKβ,SSAA
R11863	T14817	T14819	themeOf	RPS3,accumulation
R11864	T14818	T14819	locationOf	nuclear,accumulation
R11865	T14819	T14820	themeOf	accumulation,retarded
R11866	T14823	T14825	themeOf	RPS3,translocation
R11867	T14824	T14825	locationOf	nuclear,translocation
R11868	T14826	T14830	themeOf	IKKβ,expressing
R11869	T14827	T14828	themeOf	IKKβ,SSEE
R11870	T14827	T14829	themeOf	IKKβ,expressing
R11871	T14832	T14834	themeOf	RPS3,translocation
R11872	T14833	T14834	locationOf	nuclear,translocation
R11873	T14834	T14831	themeOf	translocation,affect
R11874	T14835	T14836	themeOf	IKKβ,SSAA
R11875	T14835	T14837	themeOf	IKKβ,expressing
R11876	T14838	T14839	causeOf	NleH1,inhibit
R11877	T14840	T14842	themeOf	RPS3,translocation
R11878	T14841	T14842	locationOf	nuclear,translocation
R11879	T14842	T14839	themeOf	translocation,inhibit
R11880	T14845	T14843	themeOf	IKKβ,expressing
R11881	T14845	T14844	themeOf	IKKβ,activated
R11882	T14846	T14850	causeOf	NleH1,inhibiting
R11883	T14847	T14849	causeOf	phosphorylate,thus
R11884	T14848	T14847	themeOf	IKKβ,phosphorylate
R11885	T14850	T14849	themeOf	inhibiting,thus
R11886	T14851	T14852	causeOf	IKKβ,mediated
R11887	T14852	T14850	themeOf	mediated,inhibiting
R11888	T14854	T14855	themeOf	S209,phosphorylation
R11889	T14854	T14853	partOf	S209,RPS3
R11890	T14855	T14852	themeOf	phosphorylation,mediated
R11891	T14858	T14859	themeOf	IKKβ,autophosphorylation
R11892	T14858	T14861	themeOf	IKKβ,phosphorylation
R11893	T14863	T14862	themeOf	IKKβ,32P incorporation
R11894	T14867	T14868	causeOf	IKKβ,phosphorylated
R11895	T14867	T14869	causeOf	IKKβ,phosphorylated
R11896	T14870	T14868	themeOf	RPS3,phosphorylated
R11897	T14870	T14872	themeOf	RPS3,substrate specificity
R11898	T14871	T14869	themeOf	GST-IκBα,phosphorylated
R11899	T14874	T14875	causeOf	NleH1,reduced
R11900	T14874	T14876	causeOf	NleH1,reduced
R11901	T14877	T14878	causeOf	IKKβ,mediated
R11902	T14878	T14875	themeOf	mediated,reduced
R11903	T14879	T14880	themeOf	RPS3,phosphorylation
R11904	T14880	T14878	themeOf	phosphorylation,mediated
R11905	T14881	T14882	causeOf	IKKβ,mediated
R11906	T14882	T14876	themeOf	mediated,reduced
R11907	T14883	T14884	themeOf	GST-IκBα,phosphorylation
R11908	T14884	T14882	themeOf	phosphorylation,mediated
R11909	T14885	T14888	causeOf	NleH1,mediated
R11910	T14885	T14889	causeOf	NleH1,mediated
R11911	T14885	T14887	causeOf	NleH1,mediated
R11912	T14885	T14886	causeOf	NleH1,mediated
R11913	T14890	T14889	themeOf	phosphorylation,mediated
R11914	T14891	T14888	themeOf	phosphorylation,mediated
R11915	T14892	T14886	themeOf	autophosphorylation,mediated
R11916	T14893	T14887	themeOf	autophosphorylation,mediated
R11917	T14894	T14891	themeOf	RPS3,phosphorylation
R11918	T14894	T14893	themeOf	RPS3,autophosphorylation
R11919	T14895	T14890	themeOf	GST-IκBα,phosphorylation
R11920	T14895	T14892	themeOf	GST-IκBα,autophosphorylation
R11921	T14899	T14900	causeOf	IKKβ,mediated
R11922	T14900	T14898	themeOf	mediated,inhibiting
R11923	T14902	T14903	themeOf	S209,phosphorylation
R11924	T14902	T14901	partOf	S209,RPS3
R11925	T14903	T14900	themeOf	phosphorylation,mediated
R11791	T14715	T14716	causeOf	NleH1,inhibits
R11792	T14718	T14719	themeOf	S209,phosphorylation

test2

Id	Subject	Object	Predicate	Lexical cue	Negation	Speculation
T14525	0-5	Protein	denotes	NleH1
T14526	17-21	Protein	denotes	IKKβ
T14527	46-51	Protein	denotes	NleH1
T14528	123-133	Entity	denotes	lysine 159
T14529	177-182	Protein	denotes	NleH1
T14530	183-191	Negative_regulation	denotes	inhibits
T14531	192-196	Protein	denotes	RPS3		true
T14532	197-201	Entity	denotes	S209
T14533	202-217	Phosphorylation	denotes	phosphorylation
T14534	287-296	Protein	denotes	His-NleH1
T14535	318-327	Protein	denotes	His-NleH1
T14536	361-366	Protein	denotes	NleH1
T14537	370-388	Phosphorylation	denotes	autophosphorylated
T14538	409-414	Protein	denotes	NleH1
T14539	415-426	Negative_regulation	denotes	kinase-dead
T14540	487-495	Positive_regulation	denotes	required
T14541	500-505	Protein	denotes	NleH1
T14542	509-516	Negative_regulation	denotes	inhibit
T14543	517-521	Protein	denotes	IKKβ
T14544	522-537	Phosphorylation	denotes	phosphorlyation
T14545	541-545	Protein	denotes	RPS3
T14546	549-553	Entity	denotes	S209
T14547	570-580	Gene_expression	denotes	expressing
T14548	607-612	Protein	denotes	NleH1
T14549	638-643	Protein	denotes	NleH1
T14550	644-654	Gene_expression	denotes	expression
T14551	669-676	Negative_regulation	denotes	reduced
T14552	681-688	Positive_regulation	denotes	induced
T14553	689-693	Protein	denotes	RPS3
T14554	694-698	Entity	denotes	S209
T14555	699-714	Phosphorylation	denotes	phosphorylation
T14556	773-778	Protein	denotes	NleH1
T14557	798-806	Positive_regulation	denotes	required
T14558	810-817	Negative_regulation	denotes	protect
T14559	818-822	Protein	denotes	RPS3
T14560	828-832	Protein	denotes	IKKβ
T14561	833-841	Positive_regulation	denotes	mediated	true
T14562	842-857	Phosphorylation	denotes	phosphorylation
T14563	1001-1005	Protein	denotes	NleH
T14564	1006-1015	Negative_regulation	denotes	inhibited
T14565	1016-1020	Protein	denotes	RPS3
T14566	1021-1028	Entity	denotes	nuclear		true
T14567	1029-1042	Localization	denotes	translocation
T14568	1047-1051	Protein	denotes	RPS3
T14569	1052-1061	Regulation	denotes	dependent
T14570	1068-1078	Protein	denotes	luciferase
T14571	1123-1128	Protein	denotes	NleH1
T14572	1150-1154	Protein	denotes	RPS3
T14573	1155-1159	Entity	denotes	S209
T14574	1160-1175	Phosphorylation	denotes	phosphorylation
T14575	1271-1281	Positive_regulation	denotes	stimulated
T14576	1294-1302	Positive_regulation	denotes	increase
T14577	1306-1310	Protein	denotes	RPS3
T14578	1311-1315	Entity	denotes	S209
T14579	1316-1331	Phosphorylation	denotes	phosphorylation
T14580	1348-1360	Positive_regulation	denotes	augmentation
T14581	1364-1368	Protein	denotes	RPS3	true
T14582	1369-1384	Phosphorylation	denotes	phosphorylation
T14583	1389-1396	Negative_regulation	denotes	reduced
T14584	1466-1470	Protein	denotes	RPS3
T14585	1471-1486	Phosphorylation	denotes	phosphorylation
T14586	1491-1499	Positive_regulation	denotes	enhanced
T14587	1552-1559	Negative_regulation	denotes	lacking
T14588	1560-1564	Protein	denotes	NleH
T14589	1575-1580	Protein	denotes	ΔnleH
T14590	1615-1620	Protein	denotes	NleH1		true
T14591	1660-1665	Protein	denotes	ΔnleH
T14592	1701-1708	Gene_expression	denotes	express
T14593	1743-1748	Protein	denotes	NleH1
T14594	1764-1769	Protein	denotes	ΔnleH
T14595	1792-1797	Protein	denotes	NleH1
T14596	1805-1814	Negative_regulation	denotes	abolished	true
T14597	1815-1818	Protein	denotes	TNF
T14598	1819-1826	Positive_regulation	denotes	induced
T14599	1827-1831	Protein	denotes	RPS3
T14600	1832-1836	Entity	denotes	S209	true
T14601	1837-1852	Phosphorylation	denotes	phosphorylation
T14602	1896-1903	Negative_regulation	denotes	inhibit	true
T14603	1904-1908	Protein	denotes	RPS3
T14604	1909-1924	Phosphorylation	denotes	phosphorylation
T14605	1981-1986	Protein	denotes	NleH1
T14606	2006-2014	Positive_regulation	denotes	required	true
T14607	2018-2023	Negative_regulation	denotes	block
T14608	2024-2028	Protein	denotes	RPS3	true
T14609	2029-2033	Entity	denotes	S209
T14610	2034-2049	Phosphorylation	denotes	phosphorylation	true
T14611	2103-2108	Protein	denotes	NleH1
T14612	2135-2141	Negative_regulation	denotes	impair	true
T14613	2153-2160	Entity	denotes	nuclear
T14614	2161-2174	Localization	denotes	translocation
T14615	2178-2182	Protein	denotes	RPS3
T14616	2183-2190	Positive_regulation	denotes	trigged
T14617	2198-2219	Positive_regulation	denotes	constitutively-active
T14618	2220-2224	Protein	denotes	IKKβ
T14619	2226-2230	Protein	denotes	IKKβ
T14620	2276-2286	Gene_expression	denotes	expressing
T14621	2307-2316	Protein	denotes	SSEE IKKβ
T14622	2327-2336	Positive_regulation	denotes	triggered
T14623	2342-2346	Protein	denotes	RPS3
T14624	2347-2354	Entity	denotes	nuclear
T14625	2355-2368	Localization	denotes	translocation
T14626	2378-2389	Negative_regulation	denotes	kinase-dead
T14627	2390-2394	Protein	denotes	IKKβ
T14628	2396-2400	Negative_regulation	denotes	SSAA
T14629	2421-2425	Protein	denotes	RPS3
T14630	2426-2433	Entity	denotes	nuclear
T14631	2434-2446	Localization	denotes	accumulation
T14632	2465-2473	Negative_regulation	denotes	retarded
T14633	2570-2576	Protein	denotes	ΔnleH1
T14634	2580-2585	Protein	denotes	ΔescN
T14635	2608-2616	Negative_regulation	denotes	impaired
T14636	2617-2621	Protein	denotes	RPS3
T14637	2622-2629	Entity	denotes	nuclear
T14638	2630-2643	Localization	denotes	translocation
T14639	2654-2658	Protein	denotes	IKKβ
T14640	2663-2667	Protein	denotes	IKKβ
T14641	2669-2673	Negative_regulation	denotes	SSEE
T14642	2675-2685	Gene_expression	denotes	expressing
T14643	2743-2749	Regulation	denotes	affect
T14644	2754-2758	Protein	denotes	RPS3
T14645	2759-2766	Entity	denotes	nuclear
T14646	2767-2780	Localization	denotes	translocation
T14647	2784-2788	Protein	denotes	IKKβ
T14648	2790-2794	Negative_regulation	denotes	SSAA
T14649	2796-2806	Gene_expression	denotes	expressing
T14650	2878-2883	Protein	denotes	NleH1
T14651	2910-2917	Negative_regulation	denotes	inhibit
T14652	2918-2922	Protein	denotes	RPS3
T14653	2923-2930	Entity	denotes	nuclear
T14654	2931-2944	Localization	denotes	translocation
T14655	2959-2969	Gene_expression	denotes	expressing
T14656	2970-2985	Positive_regulation	denotes	constitutively-
T14657	2985-2994	Positive_regulation	denotes	activated
T14658	2995-2999	Protein	denotes	IKKβ
T14659	3021-3026	Protein	denotes	NleH1
T14660	3042-3055	Phosphorylation	denotes	phosphorylate
T14661	3056-3060	Protein	denotes	IKKβ
T14662	3066-3076	Negative_regulation	denotes	inhibiting
T14663	3077-3081	Protein	denotes	IKKβ
T14664	3082-3090	Positive_regulation	denotes	mediated
T14665	3091-3095	Protein	denotes	RPS3
T14666	3096-3100	Entity	denotes	S209
T14667	3101-3116	Phosphorylation	denotes	phosphorylation
T14668	3179-3195	Protein	denotes	Flag-IKKβ (K44A)
T14669	3225-3234	Protein	denotes	His-NleH1
T14670	3254-3258	Protein	denotes	IKKβ
T14671	3259-3278	Phosphorylation	denotes	autophosphorylation
T14672	3297-3302	Protein	denotes	NleH1
T14673	3303-3310	Positive_regulation	denotes	induced
T14674	3311-3326	Phosphorylation	denotes	phosphorylation
T14675	3392-3396	Protein	denotes	IKKβ
T14676	3491-3496	Protein	denotes	NleH1
T14677	3503-3508	Regulation	denotes	alter
T14678	3513-3517	Protein	denotes	IKKβ
T14679	3630-3634	Protein	denotes	IKKβ
T14680	3649-3653	Protein	denotes	IKKβ
T14681	3654-3668	Phosphorylation	denotes	phosphorylated
T14682	3669-3673	Protein	denotes	RPS3
T14683	3696-3704	Protein	denotes	GST-IκBα
T14684	3822-3826	Protein	denotes	IKKβ
T14685	3832-3837	Protein	denotes	NleH1
T14686	3838-3845	Negative_regulation	denotes	reduced
T14687	3846-3850	Protein	denotes	IKKβ
T14688	3851-3859	Positive_regulation	denotes	mediated
T14689	3860-3864	Protein	denotes	RPS3
T14690	3865-3880	Phosphorylation	denotes	phosphorylation
T14691	3923-3927	Protein	denotes	IKKβ
T14692	3928-3936	Positive_regulation	denotes	mediated
T14693	3937-3945	Protein	denotes	GST-IκBα
T14694	3946-3961	Phosphorylation	denotes	phosphorylation
T14695	4038-4043	Protein	denotes	NleH1
T14696	4044-4052	Positive_regulation	denotes	mediated
T14697	4053-4068	Phosphorylation	denotes	phosphorylation
T14698	4072-4091	Phosphorylation	denotes	autophosphorylation
T14699	4095-4099	Protein	denotes	RPS3
T14700	4103-4111	Protein	denotes	GST-IκBα
T14701	4139-4144	Protein	denotes	NleH1
T14702	4185-4189	Protein	denotes	IKKβ
T14703	4195-4205	Negative_regulation	denotes	inhibiting
T14704	4210-4214	Protein	denotes	IKKβ
T14705	4215-4223	Positive_regulation	denotes	mediated
T14706	4224-4228	Protein	denotes	RPS3
T14707	4229-4233	Entity	denotes	S209
T14708	4234-4249	Phosphorylation	denotes	phosphorylation
R11659	T14528	T14527	partOf	lysine 159,NleH1
R11660	T14529	T14530	causeOf	NleH1,inhibits
R11661	T14532	T14533	themeOf	S209,phosphorylation
R11662	T14532	T14531	partOf	S209,RPS3
R11663	T14533	T14530	themeOf	phosphorylation,inhibits
R11664	T14536	T14539	themeOf	NleH1,kinase-dead
R11665	T14536	T14537	themeOf	NleH1,autophosphorylated
R11666	T14538	T14539	themeOf	NleH1,kinase-dead
R11667	T14542	T14540	themeOf	inhibit,required
R11668	T14544	T14542	themeOf	phosphorlyation,inhibit
R11669	T14546	T14545	partOf	S209,RPS3
R11670	T14546	T14544	themeOf	S209,phosphorlyation
R11671	T14548	T14547	themeOf	NleH1,expressing
R11672	T14549	T14550	themeOf	NleH1,expression
R11673	T14550	T14551	causeOf	expression,reduced
R11674	T14552	T14551	themeOf	induced,reduced
R11675	T14554	T14555	themeOf	S209,phosphorylation
R11676	T14554	T14553	partOf	S209,RPS3
R11677	T14555	T14552	themeOf	phosphorylation,induced
R11678	T14558	T14557	themeOf	protect,required
R11679	T14559	T14562	themeOf	RPS3,phosphorylation
R11680	T14560	T14561	causeOf	IKKβ,mediated
R11681	T14561	T14558	themeOf	mediated,protect
R11682	T14562	T14561	themeOf	phosphorylation,mediated
R11683	T14563	T14564	causeOf	NleH,inhibited
R11684	T14565	T14567	themeOf	RPS3,translocation
R11685	T14566	T14567	locationOf	nuclear,translocation
R11686	T14567	T14564	themeOf	translocation,inhibited
R11687	T14568	T14569	causeOf	RPS3,dependent
R11688	T14569	T14564	themeOf	dependent,inhibited
R11689	T14573	T14574	themeOf	S209,phosphorylation
R11690	T14573	T14572	partOf	S209,RPS3
R11691	T14578	T14577	partOf	S209,RPS3
R11692	T14578	T14579	themeOf	S209,phosphorylation
R11693	T14579	T14576	themeOf	phosphorylation,increase
R11694	T14580	T14583	themeOf	augmentation,reduced
R11695	T14581	T14582	themeOf	RPS3,phosphorylation
R11696	T14582	T14583	themeOf	phosphorylation,reduced
R11697	T14582	T14580	themeOf	phosphorylation,augmentation
R11698	T14584	T14585	themeOf	RPS3,phosphorylation
R11699	T14585	T14586	themeOf	phosphorylation,enhanced
R11700	T14587	T14586	causeOf	lacking,enhanced
R11701	T14588	T14587	themeOf	NleH,lacking
R11702	T14590	T14592	themeOf	NleH1,express
R11703	T14591	T14592	themeOf	ΔnleH,express
R11704	T14593	T14592	themeOf	NleH1,express
R11705	T14595	T14596	causeOf	NleH1,abolished
R11706	T14597	T14598	causeOf	TNF,induced
R11707	T14598	T14596	themeOf	induced,abolished
R11708	T14600	T14601	themeOf	S209,phosphorylation
R11709	T14600	T14599	partOf	S209,RPS3
R11710	T14601	T14598	themeOf	phosphorylation,induced
R11711	T14603	T14604	themeOf	RPS3,phosphorylation
R11712	T14604	T14602	themeOf	phosphorylation,inhibit
R11713	T14607	T14606	themeOf	block,required
R11714	T14609	T14608	partOf	S209,RPS3
R11715	T14609	T14610	themeOf	S209,phosphorylation
R11716	T14610	T14607	themeOf	phosphorylation,block
R11717	T14613	T14614	locationOf	nuclear,translocation
R11718	T14614	T14612	themeOf	translocation,impair
R11719	T14614	T14616	themeOf	translocation,trigged
R11720	T14615	T14614	themeOf	RPS3,translocation
R11721	T14618	T14616	causeOf	IKKβ,trigged
R11722	T14618	T14617	themeOf	IKKβ,constitutively-active
R11723	T14619	T14616	causeOf	IKKβ,trigged
R11724	T14619	T14617	themeOf	IKKβ,constitutively-active
R11725	T14620	T14622	causeOf	expressing,triggered
R11726	T14621	T14620	themeOf	SSEE IKKβ,expressing
R11727	T14623	T14625	themeOf	RPS3,translocation
R11728	T14624	T14625	locationOf	nuclear,translocation
R11729	T14625	T14622	themeOf	translocation,triggered
R11730	T14627	T14626	themeOf	IKKβ,kinase-dead
R11731	T14627	T14628	themeOf	IKKβ,SSAA
R11732	T14629	T14631	themeOf	RPS3,accumulation
R11733	T14630	T14631	locationOf	nuclear,accumulation
R11734	T14631	T14632	themeOf	accumulation,retarded
R11735	T14633	T14635	causeOf	ΔnleH1,impaired
R11736	T14634	T14635	causeOf	ΔescN,impaired
R11737	T14636	T14638	themeOf	RPS3,translocation
R11738	T14637	T14638	locationOf	nuclear,translocation
R11739	T14638	T14635	themeOf	translocation,impaired
R11740	T14639	T14641	themeOf	IKKβ,SSEE
R11741	T14640	T14641	themeOf	IKKβ,SSEE
R11742	T14640	T14642	themeOf	IKKβ,expressing
R11743	T14644	T14646	themeOf	RPS3,translocation
R11744	T14645	T14646	locationOf	nuclear,translocation
R11745	T14646	T14643	themeOf	translocation,affect
R11746	T14647	T14649	themeOf	IKKβ,expressing
R11747	T14647	T14648	themeOf	IKKβ,SSAA
R11748	T14650	T14651	causeOf	NleH1,inhibit
R11749	T14652	T14654	themeOf	RPS3,translocation
R11750	T14653	T14654	locationOf	nuclear,translocation
R11751	T14654	T14651	themeOf	translocation,inhibit
R11752	T14658	T14655	themeOf	IKKβ,expressing
R11753	T14658	T14657	themeOf	IKKβ,activated
R11754	T14659	T14662	causeOf	NleH1,inhibiting
R11755	T14661	T14660	themeOf	IKKβ,phosphorylate
R11756	T14663	T14664	causeOf	IKKβ,mediated
R11757	T14664	T14662	themeOf	mediated,inhibiting
R11758	T14666	T14665	partOf	S209,RPS3
R11759	T14666	T14667	themeOf	S209,phosphorylation
R11760	T14667	T14664	themeOf	phosphorylation,mediated
R11761	T14670	T14671	themeOf	IKKβ,autophosphorylation
R11762	T14672	T14673	causeOf	NleH1,induced
R11763	T14674	T14673	themeOf	phosphorylation,induced
R11764	T14676	T14677	causeOf	NleH1,alter
R11765	T14680	T14681	causeOf	IKKβ,phosphorylated
R11766	T14682	T14681	themeOf	RPS3,phosphorylated
R11767	T14687	T14688	causeOf	IKKβ,mediated
R11768	T14688	T14686	themeOf	mediated,reduced
R11769	T14689	T14690	themeOf	RPS3,phosphorylation
R11770	T14690	T14688	themeOf	phosphorylation,mediated
R11771	T14691	T14692	causeOf	IKKβ,mediated
R11772	T14693	T14694	themeOf	GST-IκBα,phosphorylation
R11773	T14694	T14692	themeOf	phosphorylation,mediated
R11774	T14695	T14696	causeOf	NleH1,mediated
R11775	T14697	T14696	themeOf	phosphorylation,mediated
R11776	T14698	T14696	themeOf	autophosphorylation,mediated
R11777	T14699	T14698	themeOf	RPS3,autophosphorylation
R11778	T14699	T14697	themeOf	RPS3,phosphorylation
R11779	T14700	T14697	themeOf	GST-IκBα,phosphorylation
R11780	T14700	T14698	themeOf	GST-IκBα,autophosphorylation
R11781	T14701	T14703	causeOf	NleH1,inhibiting
R11782	T14704	T14705	causeOf	IKKβ,mediated
R11783	T14705	T14703	themeOf	mediated,inhibiting
R11784	T14707	T14708	themeOf	S209,phosphorylation
R11785	T14707	T14706	partOf	S209,RPS3
R11786	T14708	T14705	themeOf	phosphorylation,mediated

PMC:3062687 / 19647-23992 JSONTXT

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PMC:3062687 / 19647-23992 JSON TXT