Cortical tissue was taken from newborn rats and plated (after dissociation) on commercially available substrate integrated 60-electrode array dishes (Multi Channel Systems MCS GmbH, electrode diameter: 30 μs, electrode spacing: 200 or 500 μm, material: TiN) using standard procedures described elsewhere (Eytan et al., 2004) and maintained at 37°C in an atmosphere of 5% CO2 and 95% air. Experiments were performed after a 21-day incubation period, during which time a mature densely inter-connected network forms that spontaneously exhibits a characteristic bursting activity pattern (Maeda et al., 1995; Beggs and Plenz, 2003; Eytan and Marom, 2006; Plenz and Thiagarajan, 2007). The dissociated neuronal cultures develop to exhibit a rich repertoire of spontaneous spatio-temporal activity (Wagenaar et al., 2006) that may be classified by various methods, such as an order-based representation (Shahaf et al., 2008). Electrical stimulation at different electrodes, or using different amplitudes and frequencies has distinguishable effects (Marom and Shahaf, 2002).